1. Cytoprotective Effects of Triphlorethol-A Against Formaldehyde-Induced Oxidative Damage and Apoptosis: Role of Mitochondria-Mediated Caspase-Dependent Pathway.
- Author
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Rui Zhang, In Kyung Lee, Kyoung Ah Kang, Mei Jing Piao, Ki Cheon Kim, Bum Joon Kim, Nam Ho Lee, Jeong-Yun Choi, Jinhee Choi, and Jin Won Hyun
- Subjects
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FORMALDEHYDE , *TOXICITY testing , *DNA adducts , *PROTEINS , *APOPTOSIS , *MITOCHONDRIA , *ADENOSINE triphosphate , *JNK mitogen-activated protein kinases - Abstract
The toxicity of formaldehyde (HCHO) has been attributed to its ability to form adducts with DNA and proteins. Triphlorethol-A, derived from Ecklonia cava, was reported to exert a cytoprotective effect against oxidative stress damage via an antioxidant mechanism. The aim of this study was to examine the mechanisms underlying the triphlorethol-A ability to protect Chinese hamster lung fibroblast (V79-4) cells against HCHO-induced damage. Triphlorethol-A significantly decreased the HCHO-induced intracellular reactive oxygen species (ROS) production. Triphlorethol-A prevented increased cell damage induced by HCHO via inhibition of mitochondria-mediated caspase-dependent apoptosis pathway. Triphlorethol-A diminished HCHO-induced mitochondrial dysfunction, including loss of mitochondrial membrane action potential (Δψ) and adenosine triphosphate (ATP) depletion. Furthermore, the anti-apoptotic effect of triphlorethol-A was exerted through inhibition of c-Jun NH2-terminal kinase (JNK), which was enhanced by HCHO. Our data indicate that triphlorethol-A exerts a cytoprotective effect in V79-4 cells against HCHO-induced oxidative stress by inhibiting the mitochondria-mediated caspase-dependent apoptotic pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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