1. Identification of anti-CD16a single domain antibodies and their application in bispecific antibodies.
- Author
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Zhao, Yining, Li, Yumei, Wu, Xiaoqiong, Li, Li, Liu, Jiayu, Wang, Yanlan, Liu, Yue, Li, Qing, and Wang, Zhong
- Subjects
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BISPECIFIC antibodies , *KILLER cells , *CANCER cells , *TUMOR growth , *IMMUNOGLOBULINS - Abstract
CD16a (FcγRIIIa) mediates the antibody dependent cellular cytotoxicity (ADCC) and is important for anti-tumor activities of many therapeutic antibodies. Bispecific antibody targeting natural killer (NK) cells has been studied for cancer therapy. In this work, anti-CD16a single-domain antibodies were identified from hCD16a immunized camel. Bispecific antibodies are then constructed by fusing these single domain antibodies with an anti-CEA single domain antibody. These bispecific antibodies can recruite NK cells to kill CEA-positive tumor cells, and inhibit tumor growth in vivo, suggesting that these anti-CD16a single domain antibodies are powerful tools to engaging NK cells for cancer therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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