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Development of a spectral library is a prerequisite for the higher order classification of satellite data and hyperspectral image analysis to map any ecosystem with rich diversity. In this study, sampling methodology, collection of field and laboratory spectral signatures and post-processing methodologies were investigated for developing an exclusive spectral library of mangrove species using hyperspectral spectroscopic techniques. Canopy level field spectra and leaf level laboratory spectra were collected for 34 species (25 true and 9 associated mangroves) from two different mangrove ecosystems of the Indian east coast. Post-processing steps such as removal of water vapour absorption bands, correction of drifts which occur due to the thermal properties of the instrument during data collection and smoothing of spectra for its further utilisation were applied on collected spectra. The processed spectra were then compiled as spectral library. [ABSTRACT FROM AUTHOR]