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A field survey was conducted for the sampling of Acetyl-CoA carboxylase (ACCase) inhibitor resistance littleseed canarygrass, a major weed of wheat, from Punjab, Pakistan in 2014 for confirmation of resistance. The surveyed regions encompassed four different cropping systems including rice–wheat, maize–wheat, cotton–wheat and mixed cropping. Dose–response assay was conducted for confirmation of resistance. Efficacy of herbicide mixtures including clodinafop–propargyl, metribuzin, pinoxaden and sulfosulfuron at a range of doses was investigated to manage littleseed canarygrass. Results revealed that all populations were resistant to fenoxaprop except PM-BWL-2. The higher level resistance (6.5) was found in populations collected from rice–wheat cropping system. The tested herbicide mixtures at 75% and 100% of the recommended dose of each mixture component provided the effective control of resistant littleseed canarygrass. Mixtures at 50% provided more than 80% control and reduced growth and seed production potential of surviving plants. The confirmation of ACCase inhibitor resistance as the first case of herbicide resistance in Pakistan, leads us to discourage use of ACCase inhibitor herbicides alone. However, herbicide mixtures at 75% and 100% of the recommended dose are suggested to manage this weed for sustainable wheat production in the surveyed cropping systems. [ABSTRACT FROM AUTHOR]

The aim of the present research was to modify wheat gluten by binding methionine to gluten proteins to develop bread for celiac disease (CD) patients. The highest protein content, wet gluten content, dry gluten content and sodium dodecyl sulphate-sedimentation value were shown by the wheat variety AARI-11, therefore, it was selected for gluten modification. The bound methionine to gluten proteins was found increasing along the reaction time as the reaction proceeds and at a maximum near to 60 minutes and then it starts decreasing. The lowest immunoreactivity of the modified gluten was obtained near to 60 min of reaction at pH 10. The results for immunoglobulin A (IgA) index showed that the serum of each patient had positive IgA index to gliadins from unmodified gluten, but just sera of two patients had positive IgA index to gliadins from modified gluten and when these proteins were digested, the sera of no patient's serum had positive IgA reactivity. Among physical characteristics of breads 2 hours after baking, the specific volume of the modified gluten containing bread (4.13 ± 0.14 cm3/g) was lower than the control bread (4.59 ± 0.21 cm3/g). However, bread made with modified gluten had higher specific volumes than other gluten-free breads. Texture of the modified gluten was also affected by modification. Finally, the gluten content in the modified gluten bread was 79 ppm which is under the limits set by the Codex Alimentarius for food with reduced gluten content should have from 20 to 100 ppm. The study concludes that the incorporation of steric immensity into gluten proteins in order to shun immune recognition is the most promising approach to acquire wheat-based products that are tolerated by CD patients. [ABSTRACT FROM AUTHOR]

The present study was conducted to evaluate the phytochemical profile of green grasses including barley and wheat. Green grasses were procured from Ayub Agricultural Research Institute, Faisalabad, Pakistan. All the phytochemical parameters were determined through standard methods with slight modifications. The explored parameters include proximate analysis, mineral profile, vitamin profile, and especially antioxidant profile (total phenolic contents (TPCs), free radical scavenging activity, flavonoids, and phenolic acids). The results portrayed that there was a significant difference between the proximate parameters, vitamin, and minerals profile of both barley and wheatgreen grasses. The antioxidant profile - antioxidant activity (283.5%) and free radical scavenging activity (99.29%) - of barley grass was much higher than wheatgrass. The TPCs (203.52 µmol of GAE/g) and flavonoids (161.12 μmol/L) were much higher in wheatgrass as compared to barley grass. Barley grass showed maximum contents of phenolic acids including benzoic acid (73.84 µg/g), caffeic acid (86.61 µg/g), gallic acid (6.33 µg/g), syringic acid (170.46 µg/g), p-hydroxybenzoic acid (42.52 µg/g), and Ferulic acid (1560.3 µg/g). Conclusively, barley grass showed higher antioxidant potential. [ABSTRACT FROM AUTHOR]

Einkorn (Triticum monococcum L. ssp. monococcum) is an ancient diploid wheat species with many useful traits and used as a wheat gene discovery model. In this research, a total of 41 diploid and tetraploid wheat accessions were tested using simple sequence repeat (SSR) markers. A total of 33 genotypes of T. monococcum along with four genotypes each of tetraploid wheat (T. dicoccon and T. durum) were used as plant material. The analysis utilized 10 polymorphic markers, including a total number of 41 alleles with an average frequency of 4.1 alleles per locus during exploration of the level of genetic variations. Various diversity analyses, which are the effective number of alleles (Ne), gene diversity (h), Polymorphic Information Content (PIC), and Shannon's information index (I), were performed for 10 'A' genome wheat SSR markers. The results showed a narrow variation in einkorn genotypes, supported by Analysis of Molecular Variance (AMOVA), with 66% maximum variation in all genotypes. The structure analysis divided the whole germplasm into two populations. A dendrogram was constructed to determine the genetic similarities using the unpaired group method with arithmetic averages (UPGMA), which separated tetraploid wheat from other genotypes/accessions. Principal Coordinate Analysis (PCoA) co-supported the clustering of UPGMA and structure by differentiating the diploid and tetraploid wheat. These findings will help understand the genetic relationships among these wheat accessions and their use in breeding programs in the future works. [ABSTRACT FROM AUTHOR]

The present project was undertaken with the key objective to find out relationship of HMW and LMW glutenin sub-units and immunological test with bread making quality. The antibody response assessed through animal modelling by using rabbits as test animals showed significant variation towards high molecular weight glutenin (HMG) and low molecular weight glutenin (LMG) of different spring wheat varieties. A significant and positive relationship of bread sensory attributes with antibody response against HMG was observed. The mastication (r=0.64), texture (r=0.69), taste (r=0.72), aroma (r=0.68), grain (r=0.71) and total score (r=0.83) showed highly significant association with antibody response against HMG. The loaf volume correlated linearly with that of HMW antibody response (r=0.51). The LMG antibody response was negatively correlated with flour characteristics and sensorial attributes with non significant level. [ABSTRACT FROM PUBLISHER]

The purpose of this paper is to provide a comprehensive overview of wheat protein characteristics and of enzyme-linked immunosorbent assay (ELISA) technique for wheat quality characteristics determination. Literature review is to report the identification of low and high molecular weight subunits of glutenins with ELISA. Immunological assay has been reported to be used as an alternative method to reduce the test time and cost. ELISA is an immunological technique involving an enzyme (a protein that catalyses a biochemical reaction) to detect the presence of an antibody or an antigen in a test sample. Currently available knowledge on immunochemical characteristics of wheat proteins is insufficient. That's why this review is unique in its comprehensive nature for immunochemical characteristics of wheat proteins. [ABSTRACT FROM AUTHOR]