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Measuring the methane to carbon dioxide ratio (CH4/CO2) from animals could be useful to predict CH4 yield when dry matter intake (DMI) cannot be measured. The objectives were to (1) evaluate the relationship of CH4/CO2 with CH4 yield; (2) compare the CH4/CO2 of 10–60 simulated spot-samples with the CH4/CO2 calculated with data from 48-h of respiration chamber measurements. The DMI and CH4 and CO2 emissions measured every 5–6 min in respiration chambers from a previous experiment with 70 lambs fed ryegrass substituted with 0%, 25%, 50%, 75% and 100% of forage rape were retrieved. Emission data were used to perform simulations of 10, 20, 30, 40, 50 and 60 randomly selected spot-samples per lamb. The CH4/CO2 of 20 or more spot-samples was useful to predict CH4 yield and detect differences between dietary treatments, while the precision of the prediction increased when increasing the number of spot-samples up to 50 samples per lamb. Twenty spot-samples were sufficient to obtain accurate CH4/CO2 estimates; however, variance decreased (precision improved) with an increasing number of spot-samples per lamb up to 50 spot-samples. The CH4/CO2 of 10–60 simulated spot-samples explained 61% to 66% of variation in the 48-h measured CH4 yield. [ABSTRACT FROM AUTHOR]

Light intensity plays a critical regulatory role in determining plant yield and quality, and energy consumption in plant factory with artificial light (PFAL). The effects of different light intensities (200, 300, 400, 500, and 600 µmol·m−2·s−1) of light-emitting diodes (LED) red and blue light with ratio 4:1 on growth, quality, nitrogen and amino acid metabolisms of ryegrass were investigated. The results showed the tiller number and shoot fresh weight in ryegrass were increased, while plant height and stem diameter remained stable with the improvement of light intensity. The highest levels of soluble protein, free amino acid, and DPPH free radical clearance rate in photon flux density (PPFD) at 500 µmol·m−2·s−1, and then significantly decreased with light intensity increased. The highest soluble sugar content was found at PPFD of 600 µmol·m−2·s−1, while malondialdehyde (MDA) content remained stable in all treatments. As the light intensity increased, nitrate content decreased, while nitrate reductase (NR) activity and the ammonium content increased. As light intensity was increased from 200 to 600 µmol·m−2·s−1, nitrite reductase (NiR) activity first decreased and then increased. Moreover, glutamate and cysteine contents increased first and then decreased, while glutamate synthase (GOGAT), glutamine synthase (GS), glutamate dehydrogenase (GDH), and cysteine synthases (CS) activities increased continuously with light intensity. Light intensity was significantly correlated with the parameters of growth, quality, nitrogen and amino acid metabolisms in ryegrass. It was recommended that PPFD at 400–500 µmol·m−2·s−1, photoperiod of 16 h/d was suitable for ryegrass high-efficient production in PFAL with relatively low energy cost. [ABSTRACT FROM AUTHOR]

Based on the growth-promoting effect of plant growth promoting bacteria on plants and the mobilization of Cd by citric acid, an experiment was designed in which the combined treatment of Bacillus megaterium and citric acid promoted ryegrass to repair Cd-contaminated soil. This study aimed to evaluate the effects of different treatments on the antioxidant enzyme activity, photosynthesis intensity, Cd accumulation, and rhizosphere cadmium migration under cadmium contamination conditions. And the soil morphology and structure changes were studied by infrared spectroscopy FourierTransformInfrared(FT-IR) and scanning electron microscope Energy Dispersive Spectrometer(SEM-EDS) before and after different treatments. The results show that the combined treatment of Bacillus megaterium and citric acid significantly improved the oxidative stress defense and plant photosynthesis and increased of rye biomass. rye biomass 1.28 times higher than CK treatment. Joint treatment significantly increased the amount of shoot accumulation of Cd, 2.31 times higher than CK treatment, increased the migration and accumulation of cadmium. FTIR and SEM-EDS also showed that the organic constituents such as O-H, C-O and C-N in soils as a major mechanism for mobilization of the heavy metal Cd. Thus, the combined treatment of Bacillus megaterium and citric acid can promote plant growth, improve the damage to ryegrass caused by single organic acid addition, and improve the plant extraction efficiency, which is a feasible way to repair Cd-contaminated soil through activated extraction system. The novelty of this study is the combined application of bacteria and chelating agents to ryegrass to improve phytoremediation efficiency. Bacillus giganosus has a good role in promoting the growth of ryegrass. As citrate, a small molecule chelate, can activate heavy metal cadmium and detoxify heavy metals, so it was selected. This study revealed in detail the response of ryegrass to the heavy metal Cd after exogenous addition of Bacillus gigansus and citrate, which is important for the application of cadmium removal by phytoremediation. Exogenous Bacillus megaterium has a pro-growth effect on ryegrass. The combined treatment of Bacillus megaterium and citric acid significantly improved oxidative defense mechanisms in ryegrass to alleviate Cd stress in plants. FTIR and SEM-EDS showed that the joint treatment significantly improved the soil nutrient content. Combined application of citric acid and Bacillus megaterium showed the highest removal rate of cadmium. [ABSTRACT FROM AUTHOR]

Studies with dairy cows receiving mixed ration and grazing annual pastures, which have low tiller density, are scarce. This study examined the effect of partial replacement of a total mixed ration (TMR) with annual temperate pasture. Treatments were ad libitum TMR (TMR100), 75% ad libitum TMR + ryegrass (Lolium multiflorum 'Maximus') (pTMR75), and 50% ad libitum TMR + ryegrass (pTMR50). Twelve multiparous Holstein and F1 Jersey x Holstein cows were divided into six groups (experimental units), taking account of milk production (26.6 ± 4.55 kg/day), days-in-milk (129 ± 50.8) and body weight (546 ± 30.6 kg). Treatments were compared using two 3 x 3 Latin square arangements, comprising three 21-day periods (measurements during final 5 days). Herbage DM intake increased from 4.8 kg/day with pTMR75 to 6.7 kg/day with pTMR50. Total DM intake decreased from 19.4 kg/day (TMR100), to 18.1 and 15.8 kg/day (pTMR75 and pTMR50, respectively). Milk production, energy corrected milk production (ECM), and milk fat content were similar between treatments, averaging 25.6, 28.4 kg/day and 44.6 g/ kg, respectively. Ryegrass pastures were able to replace up to 50% of TMR offered to mid lactation dairy cows with no adverse effects on ECM production. [ABSTRACT FROM AUTHOR]

Bioconversion of plant material with white-rot fungi has demonstrated improvements in in vitro digestibility, however, effects on rumen fermentation parameters and methane (CH4) production, when included as a dietary ingredient, are not clear. The aim of this study was to assess changes in rumen fermentation parameters and in vitro CH4 production by dietary inclusion of ryegrass-fescue hay biodegraded with P. ostreatus (H-PO) during 14 days of solid fermentation. The experiment used 2 diets with contrasting acid detergent fibre (aADFom) content which were added with 0, 3 and 6% H-PO. Substrates were incubated 3 times with sampling at 24 h. Results were analysed by ANOVA, and compared with Tukey's test and polynomial contrasts. Inclusion of H-PO at concentrations of 3 and 6% resulted in increased in vitro dry matter (DM) disappearance (IVDMD) by 10 and 12% respectively, whereas high concentrations of aADFom reduced IVDMD by 10% as compared to low aADFom. Methane production (mg/g DM incubated) and yield (mg/g DM degraded) were reduced by increasing aADFom and P. ostreatus biodegraded hay concentrations in the diets (p < 0.001) whereas concentrations of total short-chain fatty acids were not affected by the inclusion of biodegraded hay (p = 0.080) or aADFom concentration (p = 0.076). Increasing concentrations of biodegraded hay resulted in increased acetate (p < 0.001), and reduced propionate proportions (p = 0.021) but did not affect butyrate (p = 0.233). In conclusion the use of biodegraded hay as a pre-treated ingredient improved the utilisation of low and high fibre diets by increasing the in vitro disappearance, and decreasing CH4 emissions, without increasing the propionate proportion. The use of hay biodegraded with P. ostreatus (H-PO) improves the digestibility of ruminant diets. The use of H-PO decreases methane, and increases acetate production in in vitro conditions. The use of H-PO as an ingredient in ruminant diets could improve the sustainability of livestock systems. [ABSTRACT FROM AUTHOR]

Ctenopharyngodon idella (grass carp) is one of the most widely produced herbivorous species worldwide. However, forage feeding is not well studied in this species. This work aimed to evaluate the effect of feeding a diet based on Lolium multiflorum (ryegrass) on the growth performance and fatty acid composition in the meat of juvenile C. idella. For this purpose, juveniles were fed two different diets for 120 days: i) grains and their by-products (GD) and ii) Lolium multiflorum (PD). At 60 and 120 days of feeding, the weight gain, specific growth rate, feed conversion rate, protein efficiency, viscerosomatic index and condition factor were determined. The fatty acid profile and lipid nutritional indices of the meat were measured at 120 days. The GD group exhibited higher weight gain, specific growth rate and condition factor than the PD group. The GD group also had a higher conversion rate at the expense of greater fat content and elevated viscerosomatic index. The greater viscera development may explain the higher weight gain in the GD group. Healthy fatty acids, such as Alpha-linolenic, eicosapentaenoic, docosapentaenoic and docosahexaenoic fatty acids, were more elevated in the PD group while linoleic fatty acid (LA) was lower. Hence, LA induced a decrease in the n-6/n-3 ratio. In addition, hypercholesterolemic and atherogenetic saturated fatty acids were lower in the PD. This variation reduced the atherogenic, thrombogenicity and peroxidability indices. Therefore, better nutritional quality in the meat compensated for the lower growth of the fish on the PD. [ABSTRACT FROM AUTHOR]

Phytostabilization on sulfidic mine tailings is not yet commonly implemented in the field, making it a crucial area of focus for future reclamation efforts. This greenhouse study aimed to explore cost-effective amendments on metal(loid) immobilisation and identify grass species candidates for phytostabilization. This research found that (1) wheatgrass (Pascopyrum) and ryegrass (Lolium multiflorum) are suitable candidates for phytostabilization, and (2) it is cost-effective to reuse wastes of bauxite residue and compost to promote phytostabilization effectiveness. Compost should be carefully evaluated for phytostabilizing Zn in tailings since the Accumulation Factor and Translocation Factor of all selected species are large than 1. [ABSTRACT FROM AUTHOR]

Pasture seed samples that were harvested around 1917 in New Zealand were analysed for purity, Epichloë endophyte and insects. Samples consisted of 13 packets of seed varying from 2.2 to 15.0 g and comprising, Chewings fescue (Festuca rubra L.), cocksfoot (Dactylis glomerata L.), oat (Avena sativa L.), perennial ryegrass (Lolium perenne L.) and red clover (Trifolium pratense L.). These findings show that in the period prior to modern seed testing laboratories only four of the 13 samples (31%) could possibly have met today's seed certification standards as it relates to seed purity. When compared with published purities and weed content of seed of the era these samples appear typical. Endophyte testing on the four ryegrass seed samples and the ryegrass contaminants found in the Chewings fescue and cocksfoot samples showed sample-to-sample variation in endophyte infection from nil to 91%. One cocksfoot sample, most likely harvested from the Banks Peninsula, contained an Argentine stem weevil. Subsequent checking of entomological collection records also revealed specimens being present in the Wellington region from 1916. Together these occurrences precede the earliest published record for the species in New Zealand by ten years and suggest that it may have been widespread at that time. [ABSTRACT FROM AUTHOR]

Plantain (Plantago lanceolata L.) is a forage that produces secondary metabolites with one, aucubin, known to inhibit soil nitrification. This study aimed to quantify the exudation of aucubin and catalpol by plantain root systems in a hydroponic experiment; evaluate the effect of aucubin on nitrogen (N) leaching and nitrous oxide (N2O) emissions from urine applied to plantain and ryegrass-white clover swards; and compare N losses from a ryegrass-white clover sward after urine from cows fed plantain and cows fed ryegrass-white clover was applied. Nitrate ( NO 3 − ) leaching and N2O losses were measured in a lysimeter experiment. Catalpol, but not aucubin, was exudated by plantain roots. N2O emissions were decreased by plantain swards and by ryegrass-white clover swards to which aucubin was also applied. Aucubin had no effect on NO 3 − leaching. Urine from cows grazing plantain had no effect on N2O emissions, and N leaching when compared to the urine from cows grazing ryegrass-white clover with the same N content. We conclude the plantain sward and the aucubin applied to the ryegrass-white clover sward decreased N2O emissions via mechanisms in the soil separate from the decreased emissions resulting from the lower N concentration of urine derived from cows grazing plantain. [ABSTRACT FROM AUTHOR]

The productivity of perennial ryegrass (Lolium perenne L.) is constrained by summer water deficit. We hypothesised that selection for desirable water relations traits might partially mitigate this constraint. Genotypes of a 1970s, and two recent ryegrass cultivars were grown in 55 cm tall pots for three months and exposed to moisture deficit for four weeks, then 66 plants were assessed for shoot (SDW) and root (RDW) dry weight, leaf elongation rate (LER), specific water use (SWU), leaf osmotic potential (OP), OP change, leaf relative water content (RWC), and gravimetric soil moisture. Among the 66 genotypes, SWU ranged from 935 ± 19 to 1754 ± 74 g H2O g–1 DM. In principal component (PC) analysis, PC1 indicated greater growth and lower SWU from greater soil water extraction as the common trait association among genotypes tested and ANOVA of PC scores indicated that recent cultivars outperformed the 1970s cultivar. PC2 indicated water saving by decreased LER and SDW, with increased RDW indicative of summer dormancy. PC3 associated more negative OP with improved SWU and RWC. This experiment demonstrates not only genotypic diversity for SWU but also diversity in trait associations determining SWU, which should be considered when investigating SWU, both in ecology and agronomy research. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate forage mass, morphological, and chemical composition of diploids and tetraploids Italian ryegrass cultivars in hydromorphic soil. Eight ryegrass cultivars were evaluated in a two-years completely randomised trial with four plots per cultivar. Forage production was recorded, and samples were collected to evaluate morphological and chemical composition. The contrasts methodology was used to verify differences between diploid and tetraploid cultivars. Compared to tetraploids, the diploids had the greatest number of cuts and the largest accumulation of forage in the second year, highlighting the better adaptation of cultivars in hydromorphic soils. Tetraploid ryegrass increased leaves and reduced stem and inflorescence proportion relative to diploid ryegrass. Tetraploid cultivars had lower DM, organic matter, and acid detergent fibre content than diploid cultivars. However, there was no significant difference between cultivars associated with their ploidy for the neutral detergent fibre and estimated total digestible nutrients. Additionally, only in the second year, tetraploid had lower crude protein content than diploid ones. Tetraploid ryegrass showed be less tolerant to periods of water stress due to flooding, characteristic of hydromorphic soils, resulting in less forage production than diploid ryegrass. [ABSTRACT FROM AUTHOR]

Combining several cultivars within species in seed mixtures is often advocated as a means for improving crop or pasture performance. Herbage accumulation (HA) of perennial ryegrass-white clover pastures sown with a single cultivar representing different functional types of perennial ryegrass was compared with the HA of cultivar mixtures over three years in two contrasting environments (Waikato, dryland; Canterbury, irrigated) under dairy cattle grazing. Functional types were: mid-season flowering diploid, late-season diploid, and late-season tetraploid. Mixture treatments (n = 4 at each site) included all binary combinations plus the three-way combination. Mixture HA and the mean HA of the constituent cultivars did not differ for any of the mixture combinations in either environment, apart from winter in Waikato where the mid-diploid plus tetraploid mix out-yielded the mean of the individual cultivars and tended to increase total annual HA. The total annual HA of the mixtures was between 1.0 and 2.3 t DM/ha/year less than the HA of the highest-yielding individual cultivar included in the mixture in six of the eight mixture-environment combinations. While some benefits of mixtures for controlling grazing outcomes were observed, sowing individual high-performing cultivars is a better overall strategy than mixing cultivars to cover a spectrum of traits. [ABSTRACT FROM AUTHOR]

Muck from slurry shield tunnels (MSST) is a complex mixture. Directly transporting this muck to dumping grounds not only increases the costs but also raises environmental concerns due to its unusual properties. In this study, a formula has been studied that can turn the MSST into an environment friendly soil. The treatment process can realize the harmless disposal and effective utilization of MSST. In the laboratory study, three different types of growing media (MSST, MSST with 1% fly ash, and local garden soil) were used to grow ryegrass in a series of pot experiments, and the seed germination, chlorophyll content, root characteristics, root activity and physical and chemical properties of the growing media were examined. The results showed that MSST and MSSTF (MSST with added fly ash) can be used as a soil matrix for ryegrass because they exhibited a technical performance comparable to that of local garden soil (LGS), and the physicochemical parameters of the soil matrix were enhanced compared with the standard values of CJT 340–2011. Although the additive (fly ash) and different types of substrates can affect plant growth and the properties of harvested soils, the level of variation decreases with the stage of growth. As waste recycling is the primary objective of this work, MSSTF is the ideal substrate to use. The treatment process is easy to handle, and represents a sustainable option and an environmentally friendly, beneficial use of MSST.Implications: Muck discharged from slurry shield tunnel construction has put great pressure on the environment and construction cost because of a large number shield tunnels emerging in cities. Directly transporting the discharged muck waste to dumping site not only increases the machinery and labor costs but also raises environmental concerns. Therefore, we explore the way of recycling the discharged muck from slurry shield tunnel as a growth medium for landscaping which was proved to be feasible by a series of pot experimental study. Recycling the discharged muck from shield tunnel as the growth medium for landscaping instead of using purchased raw materials not only can dramatically reduce project costs but also can obviously minimizing the consequent environment pollution. Such technology can contribute to sustainable developments and cleaner construction of infrastructures. [ABSTRACT FROM AUTHOR]

The objective was to determine the effect of grazing fodder beet versus ryegrass-dominant pasture from mid-to-late gestation on dam and offspring performance under practical farming conditions. Twin-bearing ewes were randomly allocated to grazing regimes of either fodder beet plus ad libitum ryegrass/clover hay (FB; n = 100) or ryegrass-dominant pasture plus ad libitum ryegrass/clover hay (RG; n = 100) with three replicates per group, from pregnancy day (P) 100–110 to birth. At P135, 10 ewes per treatment were randomly selected from each replicate, slaughtered and back fat and eye muscle depth and fetal body weight and composition were measured. Samples were evaluated for selected blood metabolites. Ewe BW and BCS change and lamb growth were measured, and lamb survival from birth to weaning calculated. Compared with RG, FB ewes had lower ADG and overall lower BCS resulting from increased mobilisation of both fat and muscle. Lambs born to FB-ewes had lower BW and postnatal growth rates and increased mortality from birth to weaning (29% versus 12%). Body composition and blood parameters indicated that FB compared to RG ewes were subject to undernutrition. This study provides new insights into the consequences of FB grazing regimes, as an alternative to ryegrass pasture, on animal performance. [ABSTRACT FROM AUTHOR]

Forage yield is traditionally measured by manual harvesting, drying, and weighing and has multiple uses, including plant breeding and pasture management. The goal of this paper was to determine the accuracy of unmanned aerial vehicle (UAV)-based prediction of ryegrass percentage cover, vegetation volume, and dry matter (DM) yield in Autumn from 300 rectangular 1.5 m2 plots at a height of 20 m above ground level, compared to the current manual method. The secondary goal was to evaluate the UAV-based method for the determination of dry matter yield from five different ryegrass cultivars. A photogrammetry-based technique combined with a spectral method to determine the soil level was used to determine the percentage cover and vegetation volume of ryegrass plots, which were then used to obtain calibration curves to predict DM yield per plot. Calibration curves were obtained for five different ryegrass cultivars, with concordance between calibration curves for four of the five cultivar populations. The relationship between predicted forage volume (m3) and measured DM (g per 1.5 m2 plot) for ryegrass Populations 1,2,4,5 had an R2 = 0.61. Population 3 was different to Populations 1,2,4,5, with a two-fold difference in DM yield for the same forage volume. This demonstrated that 61% of the variance in DM yield can be explained by forage volume determined by a UAV-based photogrammetry method. We also further tested the methodology from 70 rectangular 2.4 m2 ryegrass plots in a Spring trial. The relationship between the predicted DM yield per 2.4 m2 plot (based on the average predictions from forage volume and forage area models) and measured DM yield per plot had an R2 = 0.66. UAVs can therefore increase the acquisition of field data for research studies and the management of pasture in grazed farm systems. [ABSTRACT FROM AUTHOR]

Although plant growth-promoting fungi can greatly accelerate the ryegrass bioaccumulation of cadmium (Cd), the underlying mechanisms are not yet well documented. Therefore, we performed a 20-days hydroponic experiment to investigate the effects of Aspergillus niger TL-F2 (A. niger TL-F2) and Aspergillus flavus TL-F3 (A. flavus TL-F3) on accumulation/subcellular distribution of Cd by annual ryegrass Dongmu 70 at different Cd concentrations (0, 2.5, and 5 mg L−1). Results indicated that both fungal strains promoted ryegrass biomass/growth by about 60%. Furthermore, we found that ryegrass roots (17.8–37.1 μg pot−1) had a significantly higher capability for Cd uptake than the shoots (1.66–5.45 μg pot−1) (p < 0.05). Of total Cd in ryegrass plants, 44–67% was in soluble form, 24–37% was in cell wall, and 8.5–25.5% was in organelles. Compared with non-fungus ryegrass, cell wall and soluble Cd fractions in fungus-inoculated roots increased and decreased by 13.5–44% and 21.5–26.4%, respectively. Besides, fungus inoculation generally increased the content of cell wall and soluble Cd fractions in ryegrass shoots. Altogether, the study concludes that inoculation of fungus in ryegrass is a promising approach to improve phytoremediation of Cd contaminated environments. Novelty statement Previous study by Han et al. (2018) examined the resistance of ryegrass plant to Cd stress after its inoculation with Aspergillus aculeatus. In this study, using a hydroponic experiment, we examined the effects of co-application of two species of Aspergillus fungi. i.e. A. niger TL-F2 and A. flavus TL-F3 on ryegrass growth/biomass, Cd absorption by ryegrass shoots and roots, and subcellular distribution of Cd in ryegrass roots and shoots. [ABSTRACT FROM AUTHOR]

Keywords: Clover; grass; legumes; forage; alfalfa; herbicide resistance; buttercup; chicory; proximal hyperspectral sensing EN Clover grass legumes forage alfalfa herbicide resistance buttercup chicory proximal hyperspectral sensing 249 251 3 05/17/22 20220801 NES 220801 In this special issue of NZJA, we have collected papers that focus on new science in the discipline of plant sciences. Ranunculus acris control in dairy pasture - a comparison of herbicides, plant growth promoters, a bioherbicide and pregraze mowing. Clover, grass, legumes, forage, alfalfa, herbicide resistance, buttercup, chicory, proximal hyperspectral sensing Continuing the legume theme, Gerard et al. ([7]) present an interesting data set comparing yields of eight perennial clover cultivars under field conditions over two years. [Extracted from the article]

Take-all, caused by the fungal pathogen Gaeumannomyces graminis var. tritici (Ggt) is an important root disease of cereals and grasses. An increasing occurrence of very light seeds in some New Zealand perennial ryegrass (Lolium perenne L.) seed crops may have been associated with Ggt, but any Ggt effects on grass seed yield were unknown. A glasshouse experiment, using prairie grass (Bromus wildenowii Kunth) as a model system was used to investigate the effect of Ggt on seed yield in the presence and absence of moisture stress (drought), and to determine whether strains of the fungal symbiont Trichoderma atroviride would allow the plant to better cope with both the biotic and abiotic stresses. Ggt significantly reduced seed yield by reducing the number of seeds per plant, while moisture stress significantly reduced all seed yield components. There was a significant interaction between Ggt and moisture stress because the moisture stress induced seed yield loss was greater in the presence of Ggt than in the absence of Ggt. In the absence of both abiotic and biotic stress, Trichoderma treatment had no effect on seed yield. However, Trichoderma significantly reduced Ggt root infection and this led to an increased seed yield. In the presence of Ggt and moisture stress, Trichoderma further increased seed yield by around double the increase for individual stress. Whether similar responses can be obtained in the field is currently being investigated. [ABSTRACT FROM AUTHOR]

Sandy soil is typically poor in organic matter. When areas with sandy soil are used for pasture prior to soybean cultivation, the availability of nitrogen (N) for plants is limited even further. Our objective was to evaluate if inoculation combined with N fertilization increases N content in the soil and N supply to soybean plants in post-pasture and post-soybean areas. Four experiments were conducted in degraded post-pasture and post-soybean areas in a sandy soil (Rhodustult), between 2016 and 2018, in Brazil. Treatments used a combination of inoculant (zero, 4, 8 and 12 rates ha−1) and mineral N (0, 25, 50, and 100 kg ha−1) with four replicates. It was using the soybean cultivar TMG 7062 IPRO. The post-soybean area had higher N content in the soil than the post-pasture area; 33, 32, and 21% for total N, microbial biomass nitrogen (MBN), and inorganic nitrogen (IN), respectively. Soybean shoots from the post-soybean area also had greater accumulations of N (86 kg ha−1 of N). Increased N fertilization linearly raised levels of N in the soil (total N, MBN, and IN). In both areas, the effect of inoculation was restricted by the increase of total N in the soil. Inoculation combined with moderate rates of N in soybeans (50 kg ha−1) increased levels of N in the soil and N supply to plants in post-pasture areas. In post-soybean areas, inoculation alone fulfilled the N soybean need. [ABSTRACT FROM AUTHOR]

Nutrient-use efficiency in pasture-crop rotations is not well understood. The hypothesis of this research is that nitrogen (N) cycled from the corn crop to the following pasture is not enough to supply the N demand of the pasture, independent of pasture height management. Our goal was to assess the carryover effect of N-fertilization from corn crop to a mixed pasture of black oat and ryegrass managed with two sward heights in an integrated crop-livestock system. The experimental design was a factorial arrangement (2 × 2) of sward height (10 and 25 cm) and N pasture fertilization: 0 kg N ha−1 (representing the carryover of N-fertilization from the corn crop) and 200 kg N ha−1. Accumulated forage dry matter and N concentration increased by 62% and 40%, respectively, in the pasture-fertilized compared with fertilization only in the previous crop phase. The residual effect of N applied to the previous corn crop was not enough to adequately meet the N requirements of the subsequent pasture, thereby reducing forage dry matter accumulation compared with the pasture-fertilized system. Our data suggest that fertilization of the pasture phase is key to better nutrient use efficiency in integrated crop-livestock systems. [ABSTRACT FROM AUTHOR]

The objective of this study was to investigate the relationship between plasma amino acid (AA) concentration and urinary nitrogen (N) concentration of dairy heifers offered cut plantain or perennial ryegrass-white clover pasture indoors. Twelve Jersey-Friesian heifers aged 9–10 months were equally assigned to two dietary treatments (plantain vs. perennial ryegrass-white clover pasture) in a completely randomised design. Urinary N concentration was lower in plantain fed heifers (1.4 g/kg) than in perennial ryegrass-white clover pasture fed heifers (3.4 g/kg). Feeding cut plantain or perennial ryegrass-white clover pasture did not have an effect on either plasma essential AA, non-essential AA or total AA. There was no association found between plasma AA concentration and urinary N concentration in the present study. Therefore, the urinary N concentration reduction in plantain fed heifers compared with perennial ryegrass-white clover pasture cannot be attributed by the differences in plasma AA. [ABSTRACT FROM AUTHOR]

Lolium perenne L., a common plant perennial ryegrass from Europe, northern Africa and Asia, was selected to produce biomass under field conditions. The biomass of this nonfood crop was from a highly contaminated agricultural soil by potentially toxic metals (Cd, Pb and Zn) with the aim of maintaining the agricultural vocation of these soils. In this perspective and in view of the metal concentration (Cd, Pb, Zn, macro- and micro-nutrients), the biomass of ryegrass was considered as a bio 'ore' resource and was used as the starting material for the preparation of contemporary biosourced catalysts. The heterogeneous catalyst from the current work was successfully used in the synthesis of Moclobemide, used to treat anxiety and major depressive episodes. Through this original approach, Moclobemide was synthesized in one step, in 80% yield under solvent-free conditions. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate nutrient digestibility, nitrogen balance, and purine derivative excretion from growing sheep fed on ryegrass silage (S) or ryegrass hay (H) cut at two maturity stages (21 and 35 d). In an in vivo trial, 32 Suffolk × Merino growing sheep (22 ± 2 kg) were used in a completely randomised design with eighth replications in a 2 × 2 factorial arrangement. Four diets were used containing 75% forage (S at 21 or 35 d and H at 21 or 35 d) and 25% concentrate. In an in vitro trial, a completely randomised design was used for gas production parameters. Contents of N and NDF were higher in H (30 and 674 g/kg DM) than in S (26 and 411 g/kg DM). Animals fed on S had higher digestibility (P <.008) of DM and OM (661 and 715 g/kg) than H at 35 d (557 and 596 g/kg). In vitro gas production was lower (P <.04) in H at 35 d. In vitro DM disappearance at 96 h was lower (P <.03) for H at 35 d. Excretion of allantoin (mmol/day) in urine was higher (P <.001) in S (7.91 and 3.95) than H (5.01 and 3.03) at 21 and 35 d respectively. Overall, compared to ryegrass hay, ryegrass silage cut at 21 d can be an advantageous feeding strategy for growing sheep without negative effects in nutrient intake, N-balance, and purine derivatives. Contents of N and NDF were higher in hay than in silage form ryegrass. Animals fed on silage had higher digestibility of DM and OM. In vitro gas production was lower in hay cut at 35 days. [ABSTRACT FROM AUTHOR]

In New Zealand, a commonly sown pasture combination is perennial ryegrass with white clover. The hydrothermal time model (HTT) was used to quantify germination under different temperature (T) and water potential (WP) conditions for white clover 'Nomad' and perennial ryegrass 'Stellar AR1'. Seeds were germinated in vitro under a range of T × WP combinations, and HTT models were fitted to germination time course data. Rapid germination of white clover under optimum T and WP was reflected by a much lower value for its HTT constant compared with perennial ryegrass (10.8 vs 80.7 MPa °C d). However, the lower base water potential for perennial ryegrass (−1.29 vs −0.495 MPa) and a higher threshold temperature for thermoinhibition (21.8 vs 18.3°C), meant that predicted perennial ryegrass germination was more resilient in warm dry conditions. HTT models can be used to assist decisions about the timing of perennial ryegrass and white clover sowing. [ABSTRACT FROM AUTHOR]

We tested whether selection of ryegrass for reduced root hair length and density would reduce root competition on white clover for phosphate (P). A Rhizosheath Selection Tool was used to select two ryegrass populations with contrasting root hair traits. These were grown at five soil P levels in a glasshouse experiment and rhizosheath trait selection had no effect on growth or shoot P concentrations. The ryegrass populations were also grown with white clover in soils with Olsen P12 or 19 mg L−1. In the Olsen P12 soil, clover grown with the smaller rhizosheath ryegrass had significantly heavier shoots and contained more P than clover grown with the larger rhizosheath ryegrass. In the Olsen P19 soil differences in ryegrass rhizosheath had no effect on clover growth. The results showed that under the experimental conditions, grass-clover competition for soil P could be modified by the rhizosheath traits of ryegrass. [ABSTRACT FROM AUTHOR]

Ryegrass is the primary pasture crop in New Zealand; however, despite this importance, gains in yield through conventional breeding have been marginal. Other significant crops, particularly maize and rice, have benefited from hybrid breeding programmes whereby heterosis has been exploited. Ryegrass presents many challenges to breeders wishing to produce F1 hybrid progeny on a large scale such as self-incompatibility, inbreeding depression, and a lack of genetic tools for creating and maintaining male-sterile lines for crossing. Many of these challenges have been overcome in other species, providing a basis for addressing them in ryegrass. With the advent of new technologies such as CRISPR gene editing and the genome sequence for ryegrass, F1 hybrid production is now possible on a large scale. Regulations in New Zealand surrounding new genetic tools are a hurdle which must be addressed to allow the agricultural industry in New Zealand to apply these advanced technologies. [ABSTRACT FROM AUTHOR]

In this work, the thermally modified attapulgite was prepared by high temperature modification and analyzed by scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and specific surface area test (BET). The results showed that the crystal phase structure of the thermally modified attapulgite was not changed compared with the attapulgite, but the content of the adsorption water and zeolite water was reduced, the surface crystal bundle structure was more obvious and the specific surface increased. The adsorption capacity of attapulgite to Cd could be improved after thermal modification, and the adsorption mechanism was mainly through the coordination of hydroxyl and silica hydroxyl with Cd. Added different doses of thermally modified attapulgite combined with ryegrass to remediate low and high concentrations of Cd-contaminated soil showed that the thermally modified attapulgite could reduce the longitudinal migration of Cd in the soil layer and the risk of groundwater pollution, but increase the biomass of ryegrass and lower the concentration of Cd in the shoot of ryegrass. It could significantly improve the total Cd extracted from the ryegrass both in the low and high contamination pollution soils when the thermally modified attapulgite dosage were 20 g·kg−1. The mechanism of the thermally modified attapulgite combined with ryegrass was mainly increased the soil pH and surface adsorption of Cd by thermally modified attapulgite, thereby reduced the content of exchangeable Cd in soil, improved the activity of urease and catalase in the soil and the diversity of soil microflora, so as to reduced the malondialdehyde (MAD) content in ryegrass and the stress of Cd on ryegrass, increased ryegrass biomass. Added thermally modified attapulgite could effectively solve the problem that ryegrass could not grow normally in soil with high pollution of Cd. And the total amount of Cd extracted from ryegrass increased by increasing the biomass of ryegrass per unit area. [ABSTRACT FROM AUTHOR]

Epichloë fungal endophytes that systemically colonise cool-season grasses have become an internationally well-researched symbiosis. While sexual species of Epichloë can cause choke disease of grass seed heads, asexual forms are symptomless endophytes that colonise embryos to become seed transmitted. In mutualistic associations, the grass benefits through greater tolerance of abiotic and biotic stresses. In New Zealand, at least 12 species of naturalised and native grasses have been documented as infected with a range of asexual or sexual Epichloë. The impact of endophyte-infected ryegrass and tall fescue has been significant for pastoral farmers, with Epichloë enhancing pasture performance. Production of bioactive secondary metabolites reduces herbivory by invertebrate pests but some are also toxic to grazing animals. Selected strains of Epichloë have been utilised to reduce livestock problems while retaining improved pasture performance. Documented Epichloë-infected native grasses are rare, justifying greater efforts of discovery, description and conservation as taonga (a treasure) for Māori and all New Zealanders. Given their ecological success in New Zealand ecosystems, along with economic and environmental benefits, there are good prospects to further develop both endemic and naturalised Epichloë-grass associations that may benefit other cool-season grasses and potentially deliver new beneficial properties. [ABSTRACT FROM AUTHOR]

This experiment investigated the feasibility of using herb-clover mixes to enable early weaning of lambs at ∼60 days of age. Over two years, twin lambs weighing more than 16 kg were allocated to three treatments: ryegrass pastures until 90 days-of-age, herb-clover mix until 90 days-of-age or weaned at ∼60 days of age onto herb-clover mix. In 2014, lambs offered the herb-clover mix, weaned at 60 or 90 days, were heavier at 90 days than lambs grazing on pasture with their dams. In 2015, lambs that weaned early onto the herb-clover mix were lighter at the same age as lambs that remained with their dam, but they still achieved a commercial weight. Ewes weaned early were heavier at weaning than ewes suckling lambs on ryegrass pasture in both years. Under some conditions, early weaning of lambs onto a herb-clover mix can be an effective alternative to conventional weaning on ryegrass pasture. [ABSTRACT FROM AUTHOR]

The objective of this study was to quantify amino acid (AA) profiles in plasma collected from dairy heifers grazing swards containing ryegrass/clover, chicory and plantain in autumn and spring. The study comprised a 28-day autumn trial and a 21-day spring trial using 56 Friesian x Jersey heifers. Total AA concentrations ranged from 2849 to 3165 μM in autumn and from 3195 to 3454 μM in spring, with no treatment effect. Season affected essential AA (EAA) and total AA concentrations. Total plasma AA concentrations were lower in autumn (2965 μM) than in spring (3303 μM). Based on these results, feeding dairy heifer's either pure chicory, binary mixture or pure plantain does not affect EAA and total AA of Friesian x Jersey heifers. Further, no relationship was established between AA and weight gain of heifers in this study. [ABSTRACT FROM AUTHOR]

Acidification is one of the recommended treatments to reduce NH3 emissions from animal slurry. Economically, the sulphuric acid is the most feasible agent to lower slurry pH. This study explains the impact of acidified slurry on soil and crop in a leaching trial. Pot experiments with ryegrass and cereal crops were used with 15 and 45 m3 ha−1 acidified slurry application. The effect of acidified slurry on ryegrass and maize yield was positive, increasing the yield by 40% and 20%, respectively, compared to unacidified slurry. There was a significant effect of acidified slurry on soil pH when large amount of acidified slurry (45 m3 ha−1) was used. Soil pH decreased by 0.1 units. The smaller amount of acidified slurry (15 m3 ha−1) had non-significant effect. Thus, for environmental protection purposes, it is necessary to ensure that the nutrient amounts applied with slurry, in particular easily soluble nitrogen and sulphur, correspond to the needs of the plants. Particular attention must be paid to Ca-poor soils. Sulphate ions are physically negatively absorbed in the soil, facilitating their availability in and leaching from the soil. With sulphate anions, an equivalent amount of Ca and Mg cations is always leached from the soil. [ABSTRACT FROM AUTHOR]

The study was designed to investigate nitrogen and carbon contents, nitrogen use efficiency, and antioxidants of a tolerant accession PI231578 (TOL) and a susceptible PI306292 (SUS) of perennial ryegrass subjected to 7.5 mM N (control) and 0.75 mM N (low) for 6, 12, and 20 d in a growth chamber. The SUS had decreased plant height, dry matter, shoot carbon content and showed more reductions in leaf chlorophyll and shoot N content at 6, 12 d or 20 d of low N. An increased N use efficiency was greater in the shoots of SUS during the low N treatments and in the roots of TOL at 20 d. Shoot soluble protein content (TSP) was unaffected in TOL but decreased in SUS at 20 d of low N, whereas shoot catalase activity decreased in SUS. Root superoxide dismutase and peroxidase activities increased in TOL at 20 d of low N. [ABSTRACT FROM AUTHOR]

The aim of this research was to assess the potential of using perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) forages containing plantain (Plantago lanceolata L.) (PRGWC + Plantain) to mitigate nitrate (NO3−) leaching in grazed grassland. Lysimeter studies were carried out to determine NO3− leaching losses from the PRGWC + Plantain forage, compared with the standard perennial ryegrass and white clover forage (PRGWC) under three different irrigation conditions (flood, rotorainer and pivot). Fresh dairy cow urine (700 kg N ha−1) was applied to these lysimeters in early summer (December) and late summer (February). Nitrogen (N) dynamics and the abundance of ammonia oxidising bacteria (AOB) and archaea (AOA) were also determined in companion mini-plots alongside the lysimeters. Nitrate leaching losses from the PRGWC + Plantain forage were 82% lower than those from the PRGWC forage when urine was applied in December and 74% less when urine was applied in February (P < .05). This was possibly due to the release of biological nitrification inhibitors by plantain which slowed down the nitrification process and this, combined with a lower drainage volume, was largely responsible for the lower NO3− leaching losses in the PRGWC + Plantain forages. The three different irrigation schemes did not significantly affect NO3− leaching losses under the two forage types. These results demonstrate the potential of using PRGWC + Plantain forages as an effective mitigation option for NO3− leaching in grazed grasslands. [ABSTRACT FROM AUTHOR]

The present study was carried out to find suitable spatial geometry of Melia dubia (an industrially important fast growing tree) for Cymbopogon martinii by assessing its growth, physiology, herbage, and essential oil yield and major essential oil constituents. Sole cropping (C. martinii) and silvi-medicinal systems viz., M. dubia (2x2 m)+C. martinii, M. dubia (3x3 m)+C. martinii, M. dubia (3x2 m)+C. martinii, M. dubia (4x4 m)+C. martinii and M. dubia (4x2 m)+C. martinii resulted significant (p ≤ 0.05) effect on growth, herbage yield, essential oil content and yield and major essential oil constituents of C. martinii. Height growth, number tillers, plant spread and physiological parameters were better under M. dubia (2X2 m)-C. martinii silvi-medicinal system. Total fresh, dry herbage and essential yield was higher under M. dubia (2X2 m)-C. martinii system compared to wider spatial geometries and sole cropping. Oil yield was higher under silvi-medicinal systems compared to sole cropping system. The essential oil recovery was also higher under M. dubia compared to sole cropping of C. mrtinii. Growth and yield attributes were higher at 2nd harvest compared to 1st and 3rd harvest. Geranyl acetate percentage was higher under closer spacing of M. dubia and it was less in sole crop. However, geraniol content was higher in sole crop and wider spatial geometries of M. dubia. [ABSTRACT FROM AUTHOR]

Pasture mitigation strategies are needed to reduce the negative effects of nitrate (NO3−) leaching from intensive pastoral livestock production systems. We explored the capability of pasture grasses with different cool season (winter-early spring) growth rates to reduce NO3− leaching and increase N uptake. Following a single urine application of 700 kg N ha−1, NO3− leaching loss and N uptake by Italian ryegrass and four perennial ryegrass cultivars of varying seasonal growth (heading dates from -7 to +20 days), either undersown with/without Italian ryegrass, were quantified in a 12 month lysimeter study. Italian ryegrass cv. Tabu had lower NO3−-N leaching loss and drainage, and greater N uptake and DM yield than perennial ryegrass varieties. Italian ryegrass total N leaching loss (143 kg N ha−1 yr−1) was 46-33% less (P < .05) than early and mid-season maturing Tyson, Arrow and AberDart perennial ryegrass. Italian ryegrass total N uptake (463 kg N ha−1) was 1.2-1.4 times greater than all other grass sward types. Overall, perennial ryegrass pure swards had similar N uptake and N leaching. Late-season maturing One 50 perennial ryegrass showed significantly reduced (P < .05) total N leaching loss. Undersowing Italian ryegrass into established perennial ryegrass swards resulted in lower NO3− leaching and greater N uptake than non-undersown perennial ryegrass swards, though not significant. Results confirm Italian ryegrass is a useful pasture grass option for reducing annual N leaching loss. Cool season perennial ryegrass varieties appear not to influence soil N uptake and leaching loss. [ABSTRACT FROM AUTHOR]

This study investigated the effects of three forage treatments [herbal plantain (PL), ryegrass-white clover pasture (RW) and root crop fodder beet (FB)] on ex-vivo rumen fluid fermentations. Quantitative real-time Polymerase Chain Reaction (qPCR) and PCR-single-stranded conformation polymorphism (PCR-SSCP) analyses were undertaken for aliquots collected from the fermentations. The FB treatment had the highest volatile fatty acid (VFA) and lowest pH compared with other treatments. The RW culture that had a higher fibre content, had higher Fibrobacter succinogenes(F. succinogenes) and anaerobic fungi levels when compared with the FB and PL. Protozoans were however the most abundant microorganisms in the FB cultures, and they had the highest water-soluble carbohydrate (WSC) content. The abundance of F. succinogenes increased in the RW fermentation up to 12 h, and the abundance of anaerobic fungi increased in the RW fermentation up to 24 h. In all the treatments, no effect was found on the overall abundance of bacteria. The findings confirmed that changes in rumen microbial community and fermentation products are partly related to the WSC and fibre content of two novel forages (i.e. PL and FB) increasingly used in animal production. [ABSTRACT FROM AUTHOR]

Irrigated, diverse pastures were managed under normal (grazed to 3.5 cm) or lax (grazed to 5 cm allowing ryegrass seed head development) grazing intensity, with or without mowing (to 3.5 cm), in spring. On the subsequent grazing rotation in summer, an experiment was conducted to investigate the carry-over effects of previous management on herbage regrowth and milk production. Nine groups of three Friesian × Jersey dairy cows each were randomly allocated to three replicates of three treatments: normal grazing (Norm); previously lax managed pastures (Lax); previously lax managed pastures that were pre-graze mown (Mow). Herbage in Mow treatments had a higher ME (p < 0.05) than Lax and Norm (11.7, 11.3 and 11.4 MJ ME/kg DM, respectively). There was no difference in dry matter intake (18 ± 0.30 kg DM/cow/d) or MS production (1.85 ± 0.02 kg MS/cow/d) among treatments. Results of this study indicated that milk production was not altered by grazing management. [ABSTRACT FROM AUTHOR]

The objective of this study was to assess live weight gain, animal behaviour and N excretion in the urine of dairy heifers fed herbs during autumn and spring. Sixty heifers were allocated into five dietary treatments: 100% ryegrass-white clove pasture (100RG/WC); 50% RG/WC + 50% chicory (50CH); 75% RG/WC + 25% chicory (25CH); 75% RG/WC + 25% plantain (25PL); and 50% RG/WC + 50% plantain (50PL). The 100RG/WC had the highest (5.0 times/6 h) and lowest (2.5 times/6 h) urination frequency compared with other treatments in autumn (averaged 3.5 times/6 h) and spring (averaged 4.5 times/6 h), respectively. The heifer LWG in spring was higher in 25CH (1.36 kg/day) than other treatments (averaged 1.10 kg/day). Urinary N concentration was 0.18% and 0.23% in autumn and spring, respectively, across treatments, with no difference between treatments. The study demonstrated inconsistent results of use of chicory and plantain as a mitigation tool to reduce the environmental impact of heifer rearing systems in autumn and spring. [ABSTRACT FROM AUTHOR]

At present, the remediation for organic pollutants and heavy metals co-contaminated soils is a challenge which needs to be broken through. In this study, alkyl polyglucoside (APG), citric acid (CA), and nitrilotriacetic acid (NTA) were chosen to enhance the phytoremediation of pyrene and Pb co-contaminated soils by perennial ryegrass. Through the comparison of the results with different applications, it could be found that the application of NTA was beneficial to the growth of perennial ryegrass, the underground and aboveground biomass were increased by 172.9-236.1% and 61.9-142.8%, respectively, meanwhile, photosynthetic activity of perennial ryegrass was affected positively. More importantly, the combined application of APG and NTA maximally promoted the accumulation and translocation of Pb (BF/TF, 0.44/0.61) and dissipation of pyrene (71.6%). These results indicated that the combined application of APG and NTA could be promising for future practical application of phytoremediation. However, the optimal dosage ratio of APG and NTA for phytoremediation needs to be further researched. [ABSTRACT FROM AUTHOR]

Surface spills of water produced from hydraulic fracturing can expose soil and groundwater to organics such as BTEX and naphthalene (BTEX&N) as well as high concentrations of salt. As an alternative to soil excavation, we evaluated the effectiveness of BTEX&N soil remediation using 2 grasses present in Colorado. Perennial ryegrass and foxtail barley were grown separately in pots in the greenhouse and exposed to salt or a synthesized produced-water slurry containing relevant levels of salt and BTEX&N. Plant biomass was measured 14 days post-spill, and levels of BTEX&N were quantified using GC/MS for soil, roots, and shoots at day 7 and 14 post-spill. Foxtail barley shoot growth was limited by BTEX&N, whereas perennial ryegrass shoot growth was enhanced by salt but not BTEX&N. While BTEX&N in soil associated with foxtail barley mainly decreased over time, the soil associated with perennial ryegrass mainly saw an increase in BTEX&N with time. However, further research is needed to determine the fate of BTEX&N within grasses and soil. [ABSTRACT FROM AUTHOR]

Biochar used in this study was prepared from wheat straw and enriched with 10% solutions of chemically pure salts: ammonium sulfate [(NH4)2SO4] and magnesium sulfate (MgSO4 7H2O). The aim of this study was to determine the growth response of Perennial ryegrass L. as well as identify changes in chemical and biological properties of sandy soil after the application of biochar. A significant increase in the amount of Perennial ryegrass L. biomass was determined after amending the soil with unenriched biochar and biochar enriched with (NH4)2SO4. The highest immobilization of available forms of Mg was observed for biochar enriched with (NH4)2SO4. The highest dehydrogenase activity was determined in the soil with biochar enriched with MgSO4 7H2O. [ABSTRACT FROM AUTHOR]

Herbage accumulation (HA) data from four sites were combined to (1) address the hypothesis that adjustments to the New Zealand Forage Value Index to account for grass-clover interactions are not required and (2) explore the main effects of clover and nitrogen (N) fertiliser treatments on pasture productivity. In summer and autumn, pastures based on mid-season heading ryegrass cultivars consistently supported a greater clover content than pastures based on late heading cultivars. However, the clover ×  cultivar interaction was significant in only 2 of 17 HA data sets available for analysis. Thus, the hypothesis is supported. Compared with ryegrass monocultures, mixtures yielded an additional 2.4 (in the Low-N treatment) and 1.2 (High-N treatment) t DM/ha/year. Apparent N transfer from clover to grass was 79 (Low N) and 27 (High N) kg N/ha/year, derived from estimated N fixation efficiencies of 66 (Low N) and 50 (High N) kg N/t clover DM grown. [ABSTRACT FROM AUTHOR]

Concentrations of boron, cobalt, copper, iron, manganese, molybdenum, selenium and zinc were measured in eight perennial ryegrass cultivars grown at four sites in New Zealand (Waikato, Manawatu, Canterbury and Southland). Herbage samples were collected from pure ryegrass plots, grown with two nitrogen fertiliser rates, with three sites sampled in autumn (April/May 2013) and all four sites in spring (October/November 2013). There were significant differences among the cultivars for most of the elements, but consistent patterns were not obvious due to interactions involving site, season and nitrogen fertiliser treatment. It is concluded that breeding ryegrass for variation in micronutrient concentrations is unlikely to be productive. [ABSTRACT FROM AUTHOR]

Concentrations of nitrogen, phosphorus, potassium, calcium, magnesium, sulphur and sodium were measured in eight perennial ryegrass cultivars grown at four sites in New Zealand (Waikato, Manawatu, Canterbury and Southland). For the macronutrient analyses, herbage samples were collected from pure ryegrass plots, grown with two nitrogen fertiliser rates, with three sites sampled in autumn (April/May) and all four sites in spring (October/November) of 2013. There were significant differences among the cultivars for all elements except potassium, extending the results of earlier research to contemporary commercial cultivars. It is suggested that breeding ryegrass for lowered shoot phosphorus concentration should be prioritised because of the economic and environmental benefits to be gained from farming with reduced phosphate fertiliser requirements. [ABSTRACT FROM AUTHOR]

This paper describes how the core treatments in the national experimental design (all combinations of with/without clover and high/low nitrogen (N)) were implemented at an irrigated Canterbury site and monitored for three years. There was a consistent N × clover interaction, caused mainly by substantially lower yields in the Low N without clover treatment compared with all other treatments. At the phenotypic contrast level, the main effect of perennial ryegrass heading date on white clover content was significant in four of the nine seasonal data sets available: on these occasions, mixtures based on mid-season heading cultivars had higher clover content than later season-heading cultivars. However, this difference was not sufficient to cause any clover × cultivar interactions in herbage accumulation. Hence, the hypothesis that relative yield rankings of ryegrass cultivars do not differ when ryegrass is grown in monoculture or in mixture with white clover is supported. [ABSTRACT FROM AUTHOR]

The implementation and monitoring of the treatments in the core experiment (eight perennial ryegrass cultivars grown under four combinations of plus/minus clover and high/low nitrogen (N)) at a dryland Waikato site is described. The N x clover interaction was significant in 10 of the 17 seasonal or total annual herbage accumulation (HA) data sets available, caused by substantially lower annual HA in the low N minus clover treatment compared with all other treatments. Five significant scaling-type clover × cultivar interactions in HA were evident. In all cases, HA was greater in plus clover than minus clover treatments, but more so for some cultivars than others. However, the interactions were not consistent across seasons, and different cultivars or phenotypic contrast groups were involved in many cases. The hypothesis that relative HA rankings of ryegrass cultivars do not differ when ryegrass is grown in monoculture versus mixtures with white clover is supported. [ABSTRACT FROM AUTHOR]

This experiment, implemented at a Southland site representative of the lower South Island, New Zealand, and monitored for three full years from 2012/2013 to 2015/2016, was one of four throughout New Zealand that investigated the yield and nutritive value rankings of perennial ryegrass cultivars sown with or without white clover and with high (225 kg N/ha/annum) or low (50 kg N/ha/annum) application rates of nitrogen fertiliser. High inputs of nitrogen (N) fertiliser increased annual total dry matter (DM) yields and seasonal yield in 12 out of 18 measurement occasions. Adding white clover increased both annual dry matter yield and seasonal yield in 7 out of 18 measurement occasions. An interaction between clover and N input occurred on 10 occasions. Over time, the low N minus clover treatment became progressively lower yielding compared with the other three treatments. Ryegrass cultivar by clover interactions occurred in the latter half of the experiment. When analysed using ryegrass characteristics, early- and late-heading ryegrass cultivars produced similar yields when sown without clover, but late-heading ryegrass cultivars had a higher yield than early-heading pastures when sown with white clover (P < .001). Tetraploid ryegrasses had lower yields than diploid ryegrasses when sown without clover (P = .001). This difference was reduced when white clover was included. There was no significant interaction with ryegrass morphology when sown with white clover, as dense ryegrass cultivars always had a higher yield than open ryegrass cultivars (P = .005). Interactions occurred between N input levels and ryegrass cultivar. These occurred in early spring or late spring of each year. Analysis by ryegrass characteristics identified consistent interactions. Ryegrass cultivars with a late-heading date were higher yielding than early-heading cultivars at low N inputs (P = .029), but they were not different at high N inputs. Diploid and tetraploid ryegrass cultivars were similar with low N inputs, but diploid cultivars out-yielded tetraploid cultivars at high N inputs (P = .005). Significant interactions between N inputs and white clover addition affected herbage nutritive value but this did not extend to consistent interactions with ryegrass cultivar. Invertebrate pest numbers were generally low and unlikely to have influenced clover/ryegrass interactions or cultivar ranking. Results from this site suggest that ranking of ryegrass cultivars needs to account for both the inclusion of clover and variation in N fertiliser input. [ABSTRACT FROM AUTHOR]

The development of economic indexing systems for ranking cultivars of forage species requires new knowledge of factors that may influence the scaling of agronomic data collected in controlled, small-plot evaluation trials to whole farm systems. In the case of perennial ryegrass indexing, one such knowledge gap is the effect of growing clover with ryegrass on the relative rankings of different ryegrass cultivars. The objective of the investigation reported in this series was to test whether the relative yield rankings of perennial ryegrass (Lolium perenne L.) cultivars representing the range in phenotypes now available to New Zealand farmers as a result of recent breeding differed when those cultivars were grown in monocultures (as per standard protocols for cultivar yield trialling systems such as the National Forage Variety Trials) versus mixtures of grass and clover. This paper presents the rationale for the investigation by reviewing developments in perennial ryegrass breeding and evaluation in the context of grass-clover relationships in grazed pasture systems. The papers that follow report results from a multi-year, multi-site investigation comparing the yield, nutritive value and other productivity-related variables of eight perennial ryegrass cultivars grown with or without white clover (monoculture versus mixture respectively) at two levels of nitrogen fertiliser. Key considerations in the design of the experiment on which the investigation was based are discussed. [ABSTRACT FROM AUTHOR]

A farm systems experiment comparing five pasture-based dairy systems was conducted in south-western Australia over 4 years on an annual ryegrass:subterranean clover sward. Fertiliser N inputs (0, 0.5, 1, 1.5 and 2 kg N ha−1day−1) and stocking rate (SR; 1.25, 1.50, 1.75, 2.0 and 2.25 milking cows ha−1) increased concurrently for each treatment (F1–F5, respectively). By design, milk yield per cow did not differ between treatments; therefore, the response was measured in terms of changes in herbage dry matter (DM) utilisation and nutritive characteristics, as well as differences in feed input and imported forage required. Total annual pasture utilisation (t DM ha−1) increased from treatment F1–F3 (5.1, 5.9 and 7.3 t DM−1ha−1, respectively), but responses beyond treatment F3 were not significant. Mean total N response efficiency was 9, 13, 8 and 7 kg DM per kg applied N for F2–F5, respectively, while mean marginal N response efficiency was 9, 17, −2 and 3 kg DM per kg applied N for F2–F5, respectively. The most efficient use of N fertiliser was achieved on treatment F3, where annual pasture utilisation levels in excess of 7 t DM ha−1yr−1were consistently demonstrated under the management strategies outlined, substantially greater than the local industry average. [ABSTRACT FROM AUTHOR]

This study was undertaken to study the impact of adding <75 μm elemental sulfur (ES) on P availability from a range of <250 μm ground rock phosphates (RP) namely; Minjingu (Mi) from Tanzania, Khouribga from Morocco (Mo) and Duchess (D) from Australia. Italian ryegrass (Lolium multiflorum, Thumpa tetraploid) grown in a glasshouse was used as the test crop and tops were harvested 5 times over 27 weeks. Co-granulating RP and 10.7% ES yielded 30–70% more ryegrass tops than RP alone, with the greatest effect with Mi. Fertilizer P recovery in the tops from the RP was 5.2% with D and 6.5% and 7.8% from Mi and Mo, respectively. ES addition increased this by 51% from Mo, 98% from D and 194% from Mi. Co-granulation of RP with ES has been shown to be an effective means of releasing plant available P to crops from RP with minimal fertilizer processing. [ABSTRACT FROM AUTHOR]