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To explore the effect of baicalin on human nucleus pulposus cells (NPCs) in response to interleukin (IL)-1β stimulation. Viability of NPCs was measured by cell counting kit-8 (CCK-8) assay. TUNEL staining assay and flow cytometry were performed to detect apoptotic cell death of NPCs. Western blot analysis was conducted to detect the expression levels of proteins. Enzyme-linked immunosorbent assay (ELISA) was applied for the determination of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), and IL-6. Oxidative stress indicators including reactive oxygen species (ROS) production, malondialdehyde (MDA) level, and superoxide dismutase (SOD) activity were measured. Baicalin attenuated IL-1β-caused cell viability reduction and apoptosis in NPCs. IL-1β-induced increase in Bax expression and decrease in Bcl-2 expression were attenuated by baicalin treatment. IL-1β-induced production of iNOS, COX-2, IL-6, and TNF-α in NPCs was inhibited by baicalin treatment. Baicalin treatment reversed IL-1β-induced increase in ROS production and MDA level, as well as decrease in SOD activity. Furthermore, baicalin treatment elevated the expression levels of Col II and Aggrecan and downregulated the expression levels of MMP3, MMP13, and ADAMTS5 in IL-1β-induced NPCs. A total of 402 related targets of baicalin and 134 related targets of intervertebral disk degeneration were found, and nine intersection targets were screened out. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that mitogen-activated protein kinase (MAPK) pathway was found to be involved in the effects of baicalin. Baicalin exhibited protective effects on IL-1β-caused cell viability reduction, apoptosis, oxidative stress, inflammation, and extracellular matrix degradation in NPCs. In addition, we found c-Jun N-terminal kinase (JNK) and p38 MAPK pathways as targets of baicalin through bioinformatic analysis. [ABSTRACT FROM AUTHOR]

The research of augmented reality physical tasks for human-computer collaboration is still an attractive dynamic research field. In recent years, AR Instruction supporting collaborative assembly has been developed, which can transfer graphic annotation between man and machine. However, there is little research on high precision manual assembly. This paper proposes a high-precision cognitive AR representation (M-AR) for manual operation guidance, which aims to transform a high-precision assembly relationship into a series of visual information to meet the user's cognition of high-precision task intention. Using this representation, we conducted a within-subject case study to compare it with original AR representation (original AR) and low-precision cognitive AR representation. The results showed that there were significant differences in execution time and operation errors. The user's subjective feedback also showed that M-AR supporting cognitive feedback can significantly improve a user's cognitive experience in a physical task. [ABSTRACT FROM AUTHOR]

Nucleus pulposus (NP) apoptosis is mainly charged for the pathological process of Intervertebral disc degeneration (IVDD). Our previous study revealed that Resveratrol (RSV) combined with 17β-estradiol (E2) was more effective in cutting down IL-1β induced NP cell apoptosis via PI3K/AKT pathway. The present study further evaluated the effect of RSV and E2 in the anti-apoptosis process of IVDD. Human nucleus pulposus (NP) cells culture system and IL-1β inducing apoptosis model were constructed in this research. RSV and E2 were used to inhibit apoptosis. FACS (Fluorescence-activated cell sorting) and CCK-8 (Cell Counting Kit-8) assays were respectively used to determine apoptotic incidence and cell viability of NP cells. Quantitative RT-PCR was used to determine expression of target genes in mRNA level, and western blot analysis was performed to detect the changes of related protein expression. RSV combined with E2 attenuated IL-1β-induced cell apoptosis and recovered cell viability. Blockers for mTOR and GSK-3β abated the effect of RSV and E2. RSV combined with E2 obviously increased activated P-mTOR and P-GSK-3β, which contributes to the downregulation of caspase-3. Activated P-NF-kappa B was not involved in the anti-apoptosis process of RSV and E2. Combination of Resveratrol and 17β-estradiol efficiently resisted IL-1β induced apoptosis of NP cell, mainly through PI3K/AKT/mTOR/caspase-3 and PI3K/AKT/GSK-3β pathway. [ABSTRACT FROM AUTHOR]

Research on Augmented Reality (AR)/Mixed Reality (MR) remote collaboration for physical tasks remains a compelling and dynamic area of study. AR systems have been developed which transmit virtual annotations between remote collaborators, but there has been little research on how haptic feedback can also be shared. In this paper, we present a Virtual Reality (VR)-Spatial Augmented Reality (SAR) remote collaborative system that provides haptic feedback with tangible interaction between a local worker and a remote expert helper. Using this system, we conducted a within-subject user study to compare two interfaces for remote collaboration between a local worker and expert helper, one with mid-air free drawing (MFD) and one with tangible physical drawing (TPD). The results showed that there were no significant differences with respect to performance time and operation errors. However, users felt that the TPD interface supporting passive haptic feedback could significantly improve the remote experts' user experience in VR. Our research provides useful information on the way for gesture- and gaze-based multimodal interaction supporting haptic feedback in AR/MR remote collaboration on physical tasks. [ABSTRACT FROM AUTHOR]