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This study aimed to analyze changes in growth parameters and secondary metabolites by intermittently applying ozonated water (OW) to the roots of red mustard, leaf mustard, and kale, grown in a nutrient film technique hydroponic system. During the period when the plants were cultivated for 3 weeks, OW at concentrations of 1 and 3 μL·L− 1 was flushed for 0, 1, 2, 4, and 8 minutes, twice a week, for a total of six treatments. In red mustard, the 1 μL·L− 1 OW treatment for 2 minutes resulted in the most significant increases in shoot fresh weight, shoot dry weight, leaf area, and an elevated shoot/root ratio. For leaf mustard, both the 1 and 3 μL·L− 1 treatments led to notable improvements in shoot fresh weight, total glucosinolate (GSL) content, and shoot/root ratio. During the two concentrations of OW, kale exhibited substantial significant increases in total GSL content, total anthocyanin content, total phenolic content, and shoot/root ratio. These results suggest that flushing OW to the plant roots in hydroponics systems, particularly at a concentration of 1 μL·L− 1 applied for 2 minutes, can effectively enhance shoot growth, secondary metabolite synthesis, and the shoot/root ratio in Brassicaceae plants. [ABSTRACT FROM AUTHOR]

Leafy vegetables are very healthy for human beings having high contents of phyto- and physico-chemicals and are low in fat and calories. Mountainous regions worldwide have diverse agroclimatic conditions conducive for the cultivation of different leafy vegetables. Present research study assessed six leafy vegetables for their composition at fifteen different agroecological sites. Fresh leaves of six leafy vegetables, i.e. alfalfa, water clover, spinach, leaf mustard, pennywort, and fenugreek, were collected from the mountainous region. Procured leaf samples were brought to analytical laboratory and were subjected to physicochemical and phytochemical analysis, i.e. moisture content, pH, TSS, ascorbic acid, carotenoids, phenolic content, flavonoids, and antioxidant potential. Among all the sampling sites and vegetables, a significant variation was observed for the physicochemical and phytochemical parameters. In samples procured from different agroecological sites, the better retention of quality parameters was observed among the leaf sample collected from fifteen different agroecological sites. The highest values were recorded for moisture content in spinach at site 1, for pH in leaf mustard at sites 4, 5 and 6, for TSS in spinach at sites 6 and 9, for ascorbic acid in alfalfa at site 6, for total phenolics in spinach at site 5, for carotenoid in leaf mustard at site 5, for flavonoids in fenugreek at site 5, and for antioxidant in spinach at site 6. It can be concluded that mountainous regions are rich in agroecological conditions suitable for producing leafy vegetables carrying healthful constituents for the consumption by human beings. [ABSTRACT FROM AUTHOR]

Black, brown, and white mustards are important spice and oil crops cultivated worldwide. In this study, the effect of mustard lines in terms of growth and yield under spring sown conditions during 2 years (2020 and 2021) was investigated under semi-arid climatic conditions in Ankara, Turkey. Fifteen lines and one standard were used in this study. The experiment was carried out according to the randomized block trial design with four replications. The results demonstrated statistically significant lines × years interaction for plant height, number of branches, number of capsules, number of seeds, thousand-seed weight, seed yield and crude oil yield of black, brown, and white mustard lines. The days to emergence, 4–6 leaves rosette stages, 50% flowering, 50% encapsulation, and harvest varied according to species and years. The better production year was 2021, when the highest seed yield (3080 kg ha−1) and oil yield (612 kg ha−1) were determined from the Brassica juncea-Bj3 line, while the lower production year was 2020, when the lowest seed yield (572 kg ha−1) and oil yield (133 kg ha−1) were detected for the B. nigra-Bn5 line. The B. juncea-Bj3 line for crude oil yield (612 kg ha−1) and the Sinapis alba-Sa5 line for seed yield (2282 kg ha−1) appeared to be superior in performance compared to the other lines and standards. The lines can be further exploited for breeding programs through selection and hybridization. Considering the yield data of the lines, it was detected that the lines of Brassica juncea, Sinapis alba and Brassica nigra have an important potential as a raw material resource for different industries. HIGHLIGHTS: The experimental results showed the agronomic potential of growing Brassica species under spring conditions. The yield characteristics of 15 Brassica lines were significantly affected by the environmental situation. The Brassica juncea-Bj3 and Sinapis alba-Sa2 lines were the superior lines and had a high potential for breeding new Brassica cultivars. [ABSTRACT FROM AUTHOR]

In order to maximize yield of Indian mustard (Brassica juncea (L.) Czernj. & Cosson) by optimizing sowing dates and nutrient management, a field experiment was conducted at Rajasthan Agricultural Research Institute, Jaipur, India during 3 consecutive rabi seasons (2012–13 to 2014–15). The experiment was laid out in a split plot design with four replications. The two main plot treatments comprised date of sowing (D1: IV week of October; D2: II week of November) and four sub-plot treatments comprised nutrient applications (T1: RD-N40P60K40; T2: RD-N40P60K40+RDgypsum250; T3: RD-N40P60K40+RDgypsum250+Zn and T4: RD-N40P60K40+RDgypsum250+Zn + Fe). Result showed that the seed germination, seedling growth and seedling vigor index were better in D1 sowing over D2 sowing under control as well treated conditions. Chlorophyll content increased with the application of sulfur, zinc and iron during both the sowings, with greater magnitude in D2 sowing. Seed yield and its contributing factors viz. days to flowering, plant height, shoot length, leaf area; primary branches per plant, number and length of siliqua were significantly and variably affected by different nutrient combinations. Application of T3 significantly enhanced the seed and biological yield (23.13 and 74.03 q ha−1) (16.35% and 20.63%) over T1, respectively, and T3 was statistically at par with T4. T3 also provided the highest benefit:cost (B:C) ratio (2.14) and net returns (₹ 38168 ha−1). Thus, sowing of Indian mustard in the month of October and application of RD-N40P60K40+RDgypsum250+Zn (T3) is recommended to maximize its seed yield along with higher B:C ratio and returns under semi-arid conditions of India. [ABSTRACT FROM AUTHOR]

Global warming delays the harvest of kharif crops, which delay the subsequent sowing of Indian mustard, especially in northwestern India and also causes dry winters that exacerbate challenges for the timely sowing of rabi-crops. To check this, a field experiment was conducted during the Rabi-seasons of 2018–19 and 2019–20 to assess the influence of sowing dates, irrigation, and fertilizer levels under late-sown conditions. A split-plot design was used with two sowing dates, two irrigation levels and four fertilizer levels (87.5%, 100%, 112.5% and 125% RDF; Recommended-fertilizers-dose). Crop sown in the 4th week of November significantly reduced seed (46.79 and 38.77%) and stover yield (43.72 and 41.62%) as compared to the 2nd week of November sowing during 2018–19 and 2019–20, respectively. Uptake of NPK by seed and stover was higher with the 2nd week of November sowing. The 2nd week of November sowing resulted in significant increase in water productivity by 63.72% and Benefit cost ratio (B:C) by 75.45% over the 4th week of November sowing. Irrigation at flowering stage increased the seed yield, water productivity and B:C by 11.94%, 1.72% and 9.17%, respectively, as compared to No post-sown irrigation. The biological yield of the Indian mustard increased with every increase in fertilizer doses but the response was significant up to 112.5% RDF (90 kg N, 33.75 kg P2O5 and 22.5 kg K2O ha−1). Application of 112.5% RDF increased the seed, stover yield, water productivity and B:C by 16.17%, 9.29%, 15.66% and 11.27%, respectively, over 87.5% RDF. [ABSTRACT FROM AUTHOR]

Abstract\nNOVELTY STATEMENTThe combined microbial-plant remediation has increasingly been used to remediate heavy metal-contaminated soil. Some microorganisms could enhance phytoremediation efficiency by solubilizing heavy metal and improve plant growth by producing phytohormones in the heavy metal contaminated soils. In the present study, a strong cesium (Cs)-tolerant fungal strain Paecilomyces lilacinus was identified from soil microorganisms contaminated with Cs, and the enrichment conditions for Cs were optimized. Furthermore, the effects of the A10 fermentation solution on the growth of Indian mustard (Brassica juncea L.) seedlings were investigated. The results indicated that the optimal combination of factors consisted of a culture temperature of 28 °C, pH7.0, initial concentration of Cs at 5.91 g·L−1. The maximum enrichment of Cs in the A10 was up to 75.36 mg·g−1 DW. In addition, the enrichment of Cs in Indian mustard was significantly enhanced by the application of the A10 fermentation solution, and the growth of Indian mustard was promoted under Cs stress. The present study has expanded the repertoire of microbial resources available for facilitating the Cs contaminated soil, thereby enhancing its applicability in the phytoremediation strategies.Recently, cesium (Cs) pollution has become a global environmental problem. Although much attention has been paid to combined microbe-plant remediation, there is still a lack of microbial strains with efficient Cs adsorption capacity to assist phytoremediation. The present study identified a new Cs accumulation fungus that effectively enhances the growth of Brassica juncea L. under Cs stress. In a word, the findings of this study have expanded the repertoire of microbial resources available for facilitating the Cs contaminated soil, thereby enhancing its applicability in the phytoremediation strategies. [ABSTRACT FROM AUTHOR]

Light-emitting diodes have been beneficial for plant growth, especially for microgreens in countries with low radiation, greenhouses, and chambers. However, information about how plants respond to additional light-emitting diode lighting after 12 hours of sunlight exposure in tropical areas is limited. This study examines the effects of long-duration light-emitting diodes on mustard greens in Kien Giang province, Vietnam (105°14'33.21" E; 9°91'41.13" N). The trials were conducted in the greenhouse for two growing seasons with a completely randomized design, including 0, 2, 4, and 6 hours of assembled light-emitting diodes lighting and 4 hours of commercial light-emitting diodes lighting after 6 pm, with three replications. Results showed that weather factors in season 2 favorably influenced mustard's growth. At 4 hours of lighting, commercial light-emitting diodes showed a higher chlorophyll index, flowering time, flowering rate, leaf number, and absolute growth rate of leaf number but less leaf and fresh shoot weight than assembled light-emitting diodes. Four hours of assembled light-emitting diodes positively affected most mustard growth, while 6 hours of assembled light-emitting diodes were superior for flowering aspects. Adding 4 hours of light-emitting diodes could increase leafy yield and shorten the harvesting time of mustard greens grown in greenhouses under tropical conditions. [ABSTRACT FROM AUTHOR]

In this study, the Geo-accumulation index (Igeo), Human Health Risk Assessment (HRA), and Ecological Risk Index (ERI) were utilized to examine the risks associated with the soils at the DaeyangYeongseong mine. Brassica juncea and Raphanus sativus were employed in the ecological toxicity test. In all soil samples, the mean Igeo value of arsenic measured 3.15, and cadmium measured 6.63, indicating a very high level of heavy metal contamination. The carcinogenic risk of cadmium and arsenic for adults was 4.30×10−3 and 1.43×10−5, respectively. For children, these values were 3.92 × 10−2 and 1.33 ×10−4, exceeding the acceptable level (1×10−6). In all soils, cadmium showed extremely high ecological risk levels, and arsenic had extremely high risk levels in 34.8% of the total area. This was also confirmed in toxicity assessments using plants. Therefore, arsenic and cadmium were found to be the main causes of soil contamination and ecological risk. [ABSTRACT FROM AUTHOR]

AbstractPhytoextraction is an attractive method to extract excess soil phosphorus (P) builds up from the soils. The crops potential to extract an enriched P in soil is still lacking. Therefore, four crops were grown in three different soils with variable P levels (0 to 200 mg P/kg soil) and screened for P acquisition potentials. Total P accumulation in roots and shoot was high with enriched P levels in all soils, for example in soil A at 200 mg P/kg soil, the values were 0.44% for cucumber (Cucumis sativus), 0.40% for sunflower (Helianthus annus), and ∼0.30% for cabbage (Brassica oleracea) and 0.20% for Indian mustard (Brassica juncea). The cucumber and sunflower showed a greater biomass production (>6.0 g/pot) with enriched P levels and exhibited more prominent root characteristics, suggests more favorable for P acquisition from the P enriched soils. More prominent enhanced phosphomonoesterase and phytase activities in these crops possibly contributing to their P accumulation ability under high soil P conditions. This study shows that, cucumber and sunflower can generate high dry matter content and can accumulate greater quantity of P from the soils through different mechanisms and they can be potential candidates for phytoextraction of P from the soil P enriched areas. [ABSTRACT FROM AUTHOR]

Broomrape (Phelipanche ramosa) is an obligate root parasitic plant that is known to infest many important crops. It is emerging as a serious biotic threat for Brassica juncea cultivation in India. There is very limited information available about broomrape species and its infestation in B. juncea. Hence, the present study encompassed a comprehensive five-year field survey spanning from 2018 to 2023 of 47 different B. juncea fields in the states of Rajasthan and Haryana in India. The level of broomrape infestation varied from season to season and field to field. In the B. juncea fields, broomrape with blue/violet color corolla, along with occasionally white color type were also noticed. The hairy anther, corolla shape and use of mitochondrial DNA-specific molecular markers revealed that both these broomrape types belong to Phelipanche ramosa. Scanning electron microscopy (SEM) analysis showed that P. ramosa seeds have varying shapes and seed coat have fibrillar periclinal cell wall. In addition to all the known host species for P. ramosa till date, we also identified a new host, a wild Brassicaceae weed species; Lepidium didymum in Haryana. The presence of an alternative weed host may help P. ramosa survive even in the absence of major crop host species. Overall, the present study revealed some insights into the P. ramosa-B. juncea host-parasite complex and its effect on B. juncea which could be further investigated in more detail. [ABSTRACT FROM AUTHOR]

Microbial community in the rhizosphere of plant significantly influences growth and development of a plant. As the soil vary in microbial composition due to interaction of soil with plant roots and its exudates, the role of soil in shaping microbial diversity of a crop rhizosphere is least studied. In present study, Mustard (Brassica juncea cv Pusa Mehak) was grown in three different soil viz. Vertisols, Inceptisols and Alfisols, and the bacteria were isolated form root tissues and rhizoplane to study their plant growth promoting (PGP) attributes and diversity using BOX-Polymerase chain reaction (PCR) fingerprinting technique, as influenced by soil types. Form threes soil orders, total 37 isolates were obtained of which, 19 could grow on nitrogen free medium, 6 solubilized P from tricalcium phosphate, 2 isolate solubilized potassium from glauconite and 8 had zinc solubilizing ability with the highest zinc solubilization index of 3.84 by isolate VRB-7. Based on the fingerprint analysis, differences occurred in the endophytic bacteria of same host grown in different soil types suggesting influence of soil types in colonization of microbes in rhizoplane and endorhizosphere. The common organisms obtained from host grown in different soil types indicating host genotype driven assembly of bacteria. Similarity in the isolates on rhizoplane and endorhizosphere indicated endophytic colonization of the isolates capable of firmly adhering to the rhizoplane. The findings of this study will help in formulation of crop specific bioinoculant for specific soil types and edaphic properties. [ABSTRACT FROM AUTHOR]

Arid and semi-arid regions are having huge potential for agricultural production but also have limitations of salinity and alkalinity. By mediating rhizospheric environment with the application of organic and inorganic nutrient sources, Indian mustard yield could be enhanced in these areas. For this, a field experiment was conducted to study the effect of organic (FarmYard Manure and vermicompost) and inorganic (urea) amendments with irrigation water of different sodium absorption ratios (SAR; 6, 10, 20 and 30). The experiment was laid out in a split-plot design (SPD) with 24 treatment combinations keeping different SAR in main plots and organic and inorganic nitrogen sources in sub plots. Experiment results revealed that increasing SAR levels reduced the availability of major plant nutrients, i.e. nitrogen, phosphorus and potassium. The soil organic carbon mineralization rate and Indian mustard seed yield were also deceased with increasing levels of SAR. Application of nitrogen through farm yard manure (FYM) and vermicompost decreased the electrical conductivity (EC), pH, and enhanced the cation exchange capacity (CEC) of soil solution. Application of 50% recommended dose of nitrogen (RDN) through urea and 75% through vermicompost produced maximum seed and stover yield of Indian mustard. Thus, supply of recommended dose of nitrogen as inorganic (50%) and organic (75%) sources improved the soil health and Indian mustard yield of arid lands with sodic irrigation water. The use of organic sources in place of sole application of N fertilizer may be a potential management option in sodic groundwater irrigated areas for enhancing mustard crop yield. [ABSTRACT FROM AUTHOR]

In this study, the morphological and physiological responses of Brassica juncea to the stresses of Cadmium (Cd) and trichlorfon (TCF), and the phytoremediation potential of B. juncea to Cd and TCF were investigated under hydroponics. Results showed that Cd exhibited strong inhibition on biomass and root morphology of B. juncea as Cd concentration increased. The chlorophyll a fluorescence intensity and chlorophyll content of B. juncea decreased with the increased Cd concentration, whereas the malondialdehyde and soluble protein contents and superoxide dismutase activity increased. TCF with different concentrations showed no significant influence on these morphological and physiological features of B. juncea. The biomass and physiological status of B. juncea were predominantly regulated by Cd level under the co-exposure of Cd and TCF. B. juncea could accumulate Cd in different plant parts, as well as showed efficient TCF degradation performance. A mutual inhibitory removal of Cd and TCF was observed under their co-system. The present study clearly signified the physiological responses and phytoremediation potential of B. juncea toward Cd and TCF, and these results suggest that B. juncea can be used as an effective phytoremediation agent for the Cd-TCF co-contamination in water. [ABSTRACT FROM AUTHOR]

Rapeseed, the second-most-important vegetable oil source, is cultivated in various areas of India where both groundwater and soil are contaminated with fluoride (F–). Furthermore, the frequent use of F– contaminated groundwater for irrigation leads to accumulation of F– in surface and sub-surface soil. The study aims to compare the morphological and biochemical changes in Brassica juncea L., the variations in its fatty acids (FAs) composition and oil yield, under two regimes of F– contaminated soils: (i) pre-contaminated soil (Tr) and (ii) irrigation with F– contaminated water (Ir). The level of F– (µg g−1) in the plant tissues (root, leaf, and grain) was significantly higher in Ir_10 (18.3, 14.7, and 2.8, respectively) than in Tr_10 (4.3, 2.6, and 0.77, respectively), while the oil yield was significantly lower with Ir_10 (19.5%) than with Tr_10 (44.9%). The phytoremediation potential of F– by Brassica juncea L. is greater in Tr regime than in the Ir regime. The erucic acid content (%), which is detrimental to cardiac health, increased to 67.37% (Ir_10) and 58.3% (Tr_10) from 57.73% (control). Thus, the present study shows that irrigation with F– contaminated water results in greater toxicity and accumulation in plants and is not safe for human health. Irrigation with F– contaminated water results in a greater accumulation of F– in mustard than cultivated on pre-contaminated soil. The level of erucic acid in mustard oil enhances against F– exposure. [ABSTRACT FROM AUTHOR]

Leaf mustard (Brassica juncea L.) is a variety of mustard; however, a thorough evaluation of its salt tolerance and screening the reliable traits for evaluating salt tolerance is required. This study was designed to evaluate the responses of five leaf mustard cultivars which were selected from 146 varieties to 0, 50, 100, 150, and 200 mmol L−1 NaCl at the germination and seedling stages. Compared with the control, NaCl treatments significantly decreased hypocotyl length, radicle length, fresh weight, and chlorophyll content in a few cultivars, while low NaCl concentration triggered slight increase in the three germination indexes. The relative water content was not significantly different among the control and treatments. Compared with the control, NaCl treatments, especially 200 mmol L−1 NaCl, significantly increased root/shoot, chlorophyll a/b (Chl.a/b), electrical conductivity (EC), proline content, and malondialdehyde content (MDA). Principal component analysis (PCA) identified root length, hypocotyl length, and shoot weight as the most significant traits for screening inter-accession variability of leaf mustard in the future. Based on the membership function values (MFV) of 16 traits, cultivar C (Big Leaf Oil Mustard 381) exhibited stronger salt tolerance than other cultivars. In contrast, cultivars B (Lilong Kejia Mustard) and D (Lace Sweet Mustard 353) were sensitive to salt stress among the five mustard varieties. [ABSTRACT FROM AUTHOR]

Unrestricted disposal of tannery solid waste (TSW) into agricultural soils has resulted in the contamination of heavy metals (HMs) such as chromium (Cr) cadmium (Cd), Copper (Cu), and Zinc (Zn) along with the severe potential to degrade the environmental quality around the world. In the present study, a combined phyto- and myco-remediation strategy was evaluated to enhance the growth, ionic contents, and phytoextraction potential of Brassica juncea and Vigna radiata for HMs from TSW-contaminated soil. A pot experiment was conducted in the greenhouse using single or combined inoculation of Trichoderma pseudokoningii (Tp) and Aspergillus niger (An) in B. juncea and V. radiata under TSW-contaminated soil at different doses (0, 50, and 100%). The results showed that the growth parameters of both B. juncea and V. radiata were severely affected under 50 and 100% TSW treatment. The combined inoculation of both the fungal species ameliorated the positive impacts of 50 and 100% TSW application on growth and ionic contents accumulation in B. juncea and V. radiata. The combined application of An + Tp at 100% TSW enhanced the shoot length (87.8, 157.2%), root length (123.9, 120.6%), number of leaves (184.2, 175.0%), number of roots (104.7, 438.9%), and dry weight (179.4, 144.8%) of B. juncea and V. radiata, respectively as compared to control with any fungal treatment at 100% TSW. A single application of An at different doses of TSW enhanced the metal concentration in B. juncea, whereas Tp increased the concentration of the metals in V. radiata. The concentration of Cr in roots (196.2, 263.8%), shoots (342.4, 182.2%), Cu in roots (187.6, 137.0%), shoots (26.6, 76.0%), Cd in roots (245.2, 184.6%), shoots (142.1, 73.4%), Zn in roots (73.4, 57.5%), shoots (62.9, 57.6%), in B. juncea were increased by the application of An at 50 and 100% treatment levels of TSW, respectively compared to control (C). Moreover, the HMs (Cr, Cu, Cd, and Zn) uptake was also improved under 50 and 100% TSW with the combined inoculation of Tp + An in both B. juncea and V. radiata. In conclusion, the combined inoculation of Tp + An was more effective in metal removal from TSW-treated soil. Limited studies have been conducted on filamentous fungi systematically under metal-contaminated sites for their diversity, metal tolerance, and their potential in enhancing the phytoremediation potential of different crop plants. In the present study, single and/or combined inoculation of fungal strains was found effective in alleviating different metals stress in tannery solid waste contaminated soil by improving defense mechanisms and plant growth due to the association between fungal strains and plants. The combined application of both fungal strains had an additive effect in enhancing the bioaccumulation capacity of B. juncea and V. radiata compared to their single inoculation. [ABSTRACT FROM AUTHOR]

The Asian honeybee (Apis cerana) occurs across the south, southeast and east Asia. The species is utilised in apicultural practices. But the available documents about their floral resources, especially pollen sources, are limited. However, it is a mandatory requirement in a sustainable honeybee management programme. In the present study, we conducted palynological analyses of pollen loads and field surveys to determine pollen sources for the honeybees in West Bengal, India. The honeybee species is regarded as a generalist forager with significant floral constancy supported by homogenous pollen loads with diverse pollen types (here 58). Important pollen-contributed plants were Acacia auriculiformis, Brassica juncea, Cocos nucifera, Eucalyptus sp., Neolamarckia cadamba, Peltophorum pterocarpum, Sesamum indicum and Tridax procumbens. In the semi-urban area of Midnapore town, honeybees mainly depend on planted trees and weeds for pollen sources. While in rural agricultural belts, they also visited wild trees and several cultivated crops. The bee-visited plants are diverse types regarding plant habits, flowering phenology and floral characteristics. These data will be helpful in the management of Asian honeybees, providing pollination services to cultivated crops and thereby enhancing crop yield. [ABSTRACT FROM AUTHOR]

Study was aimed to evaluate the effect of rhizobacteria on physiological attributes, agro-morphological-traits and yield of mustard under rainfed, one irrigation and two irrigation, at two different agro-climatic locations. At both locations, plant height, yield and physiological attributes – relative water content, membrane stability index and chlorophyll content – were adversely affected under rainfed condition; single irrigation had beneficial effect on these parameters. Two irrigations did not have similar impact at both locations. Inoculation with all the rhizobacteria improved yield, yield components and most of the assayed physiological attributes. The highest seed yield at Location I (2410 kg ha−1) and Location II (2130 kg ha−1) was obtained due to the inoculation with rhizobacteria Bacillus casamancensis MKS 6 and Bacillus sp. MRD 17, respectively; there was an increase in mustard seed yield by 650 kg ha−1 and 430 kg ha−1 compared with uninoculated treatment at Locations I and II, respectively. Correlation analysis indicated strong positive correlation between most of the plant biometric traits and plant physiological attributes. Hence, due to the application of rhizobacteria, plantphysiological attributes were improved, as a result, crop growth and yield were enhanced. Thus, these rhizobacterial strains can be used as bioinoculants to alleviate the adverse effects of drought and improve crop productivity. [ABSTRACT FROM AUTHOR]

Brassica juncea and B. napus species are predominantly self-pollinated. Parchment paper or muslin cloth bags are used for controlled selfed or cross-seed production. We evaluated three non-woven synthetic pollination control bags (PCBs) for two years as replacement for the commonly used PCBs. The new synthetic PCBs were fully pollen proof since the molecular marker analysis of the random seeds set on two cytoplasmic male sterile (CMS) lines isolated with PCBs were all maternally originated. DWB03 bags returned 47% significantly more seeds per bud than the parchment paper bags. Larger DWB03 bags showed 38% higher mean seed yield (g/plant) than the muslin cloth bags. Re-used DWB03 bags were statistically on par with new bags for the seed yield per plant. On average, DWB03 bags produced 57% more hybrid seed set on CMS lines than the muslin cloth bags. The micro-climate in DWB03 bags appeared to be more conducive for good seed set as compared to the outside and muslin cloth bags. Non-woven PCBs offer better options for replacing paper or muslin cloth bags in the breeding of Brassicas. [ABSTRACT FROM AUTHOR]

Soil columns were designed to investigate the vertical migration of Cd in Indian mustard (IM) and tall fescue (TF). The TF biomass was greater than the IM biomass, and the Cd content in IM was higher in the shoots but lower in the roots than that in TF. Both IM and TF released N and absorbed P and K during outdoor growth, differing from the results of the previous experiment in which plants were grown in greenhouses. TF was more absorbent and had less upward attraction than IM. The IM soil was more favorable for Cd precipitation than was the TF soil. Leaching remained the dominant effect, with only 2.28–3.40% and 2.65–3.90% of Cd absorbed by IM and TF, respectively. The present study on the vertical migration of Cd provides new insights into the phytoremediation mechanisms of IM and TF. Vertical migration rate of Cd in soil was calculated. Cd precipitation in IM soil was greater and more excellence than TF soil. TF was more absorbent and had less upward attraction than IM. Leaching remained the dominant effect with only small absorb. [ABSTRACT FROM AUTHOR]

Lack of genomic resources in sunn hemp (Crotalaria juncea L.), a leguminous bast fiber crop, is one of the major constraints for germplasm characterization and genetic analysis. We identified non-redundant sets of a variety of molecular markers from the de novo flower-bud transcriptome of sunn hemp. These molecular markers included 1683 simple sequence repeats, 4759 intron length polymorphism markers primarily from protein-coding genes, and 3309 single nucleotide polymorphism allele-specific markers. All of these marker resources have been integrated into a simple database for easy access. To evaluate marker efficiency and polymorphism information content of the sampled primers, a subset of SSR and intron length polymorphism markers were validated in sunn hemp germplasm accessions to obtain average PIC values of 0.26–0.32. The SSR genotyping profiles also aided in the genetic distance matrix-based clustering of sunn hemp germplasm accessions. The ability of these molecular markers to successfully amplify in cross-genera bast fiber crops further demonstrated their effectiveness in genotyping. Thus, the current study describes the identification of previously undisclosed endogen)ous molecular markers in sunn hemp, which have potential applications in genetic studies and breeding in sunn hemp for bast fiber improvement. [ABSTRACT FROM AUTHOR]

Understanding foraging preferences of honey bees (Apis mellifera L.) is essential to maneuver pollinator movement to enhance outcrossing and hence the crop yields. In this communication, we report the role of floral volatile organic compounds (VOCs) in structuring foraging decisions of honey bees. Supercritical fluid extraction of VOCs, coupled with gas chromatography-mass spectrometry, revealed 75 VOCs. Of these, 19 were strong attractants and 8 were strong repellants. Moreover, we detected significant, but variable, species- or genome-level differences in floral scents. Honey bees avoided Brassica juncea cv. PBR357 (0.81 bee visits/sq m/min) and B. carinata cv. PC5 (0.77 bee visits/sq m/min), which possessed peak concentrations of antifeedants or repellents (dibutyl phthalate and 9-octadecenoic acid) in their floral volatiles. B. napus cvs. GSC6 and GSC7 attracted the highest number of bee visitations (1.07 and 0.99 bee visits/sq m/min, respectively), as these possessed VOCs with honey-fruity odor in high amounts and negligible levels of antifeedants. Our results highlight that variability in floral reward chemistry should be considered when breeding crops that are frequently visited upon by bee pollinators to support bee pollinators, in general, and honey industry, in particular. [ABSTRACT FROM AUTHOR]

Study of environmental detoxification of heavy metals in flood & drain aquaponic system based on biofloc technology (BFT) has been carried out. In this system used Tilapia (Oreochromis niloticus) and Samhong Mustard (Brassica juncea L). This system consists of fish pond tank (FPT), mechanical filter tank (MFT), biological filter tank (BFT), storage tank (ST), and hydroponic plant circuits (HPC), and supported by a recirculation aquaculture system (RAS) and aeration to supply oxygen. Based on research conducted, obtained Cu (0.0036–0.02260) mg/L, Fe (0.5269–1.1013) mg/L, and Zn (0.0215–0.2581) mg/L. Although only Cu concentrations do not exceed the quality standard, the growth of Tilapia and Samhong Mustard is fast, as well as high fish survival (> 95%). The high concentration of Fe and Zn, presumably because it comes from fish food. Research on the application of biofloc technology in aquaponics systems on heavy metals has not been widely carried out, so the beginning of this research needs to be followed up with a more detailed study. [ABSTRACT FROM AUTHOR]

Research on the use of Biofloc Technology (BFT) in Flood & Drain (F&D) Aquaponic Systems has been carried out, to improve water quality parameters (pH, temperature, DO, COD, and BOD. In this system used Tilapia (Oreochromis niloticus) and Samhong Mustard (Brassica juncea L). This system consists of Fish Pond Tank (FPT), Mechanical Filter Tank (MFT), Biological Filter Tank (BFT), Storage Tank (ST), and Hydroponic Plant Circuits (HPC), and supported by a recirculating aquaculture system (RAS) and aeration to supply oxygen. Based on research conducted, obtained pH (7.7–8.7), temperature (25–30)°C, DO (5.5–7.7)mg/L, COD (9.638–25.9493)mg/L, BOD (0.4816–4.6408)mg/L. The results of this study were compared with Indonesian Government Regulations No.82 of 2001) and compromised water quality (Sommerville et al., 2014; Sallenave, 2016; Anonymous 2014). The results were pH, temperature, DO, COD, and BOD did not exceed the quality standard limits. Optimal water quality in this BFT system greatly supports the growth and survival of fish, plants, and bacteria, so the application of biofloc in an F&D aquaponic system needs to be done. [ABSTRACT FROM AUTHOR]

A pot experiment was designed to assess the effects of different rootstocks on cadmium (Cd) accumulation and the growth of the post-grafting generations of Brassica juncea var. megarrhiza. Four treatments, including ungrafted and B. juncea var. megarrhiza as the scion grafted on the rootstocks of Brassica napus, Brassica chinensis and Brassica campestris respectively, were analysed. The three grafting treatments decreased the content of Cd in the first post-grafting generations of B. juncea var. megarrhiza but increased the length of roots, tuberous roots and leaves, and tuberous root diameters, as well as the biomass of the various organs and edible parts. In addition, the grafted rootstocks could enhance the level of photosynthetic pigments and the activity of peroxidase but reduce the activities of superoxide dismutase and catalase and the soluble protein content of the first post-grafting generations of B. juncea var. megarrhiza. The different rootstocks grafts could also decrease the available concentration of Cd in the soil but increase the soil pH. Therefore, the grafting could decrease the accumulation of cadmium in the post-grafting generation of B. juncea var. megarrhiza, and the graft on B. campestris was the most effective. [ABSTRACT FROM AUTHOR]

White rust caused by Albugo candida, an oomycete pathogen, is a devastating disease of Brassica juncea (Indian mustard) worldwide. There is a need to screen virulent white rust isolates to challenge the developed white rust–resistant B. juncea cultivars to screen their resistance potential. The current study explores pathogenic and biochemical response of Indian mustard to white rust isolates collected from three different geographic locations of India. The observations refine our understanding of the disease severity in India. Disease progression and biochemical responses were studied in the cotyledonary as well as true leaf stage of the B. juncea cultivar Varuna at different time points. The biochemical findings highlight the fluctuation of significant biochemical parameters such as total proteins, sugars, and phenols, superoxide dismutase, and hydrogen peroxide during the A. candida infection in B. juncea. [ABSTRACT FROM AUTHOR]

Interspecific hybridisation can fuse different genomes into a hybrid that could derive possible a novel germplasm for vegetable improvement. Tuber mustard (Brassica juncea Coss. var. crassicaulis Chen et Yang, AABB) and kohlrabi (Brassica oleracea L. var. caulorapa, CC) belong to different species of stem vegetables in Brassica. In present study, interspecific hybridisation between tuber mustard and purple kohlrabi was conducted successfully. Through ovary culture, four putative interspecific hybrids were obtained and three of them were identified as true hybrids by morphology, cytology and molecular analysis with the hybrid acquisition frequency of 0.68%. After chromosome doubling, one hybrid doubled completely as allohexaploidy (AABBCC) with 54 chromosomes, seven hybrids (ABC) with 27 ones non-doubled, and four hybrids doubled partially as the chimeras with above two types of chromosome. The allotriploids exhibited low fertility with vigour pollen rate of 7.54% and low seed-setting rates of 0.06, however, their allohexaploids exhibited high fertility with vigour pollen rate of 51.74% and high seed-setting rate of 5.88. This allohexaploid hybrid showed unique phenotypes with the normal bigger anther, better developed pods, larger expanding stem than the allotriploid ones. The above two types of hybrids will act as new germplasm of Brassica stem vegetables in breeding programme. [ABSTRACT FROM AUTHOR]

Soil mercury (Hg) contamination has long been a problem that threatens the environment and organisms. Thus, exploring the migration and transformation of Hg from soil to vegetable is vital. In this study, different Hg solutions were employed in greenhouse experiments. The Hg contents in soil and vegetable samples were determined by inductively coupled plasma – mass spectrometry. The results showed that the Hg accumulation depended on its concentration and form. A greater accumulation of inorganic Hg was observed in the roots (2.2–27.4 mg/kg) than in the aerial parts (0.6–6.0 mg/kg). A similar trend was also found in the methyl mercury (MeHg) and ethyl mercury (EtHg) irrigated groups. The MeHg contents were 0.7–8.0 μg/kg in roots and 0.1–3.7 μg/kg in aerial parts. The EtHg concentrations were 0.5–3.3 μg/kg in roots and 0.3–1.1 μg/kg in aerial parts. The translocation factors of inorganic Hg and organic Hg for both vegetables in most treatments were less than 0.5, which suggested that Hg primarily accumulated in the roots. The biological concentration factors of inorganic Hg were less than 0.4, and those for MeHg and EtHg were between 0.4 and 0.95, which indicated that the vegetables were more likely to absorb organic Hg from the soil. The methylation of inorganic Hg occurred in the roots. The potential hazards of Hg on food safety and human health must be considered in regions with serious Hg pollution. [ABSTRACT FROM AUTHOR]

Biochar can enhance the phytoremediation of copper-contaminated soils by improving soil quality and increasing plant growth. However, the impact of biochar varies with the biomass feedstock and soil condition. Our study investigated the effect of biochar from orange bagasse-OBB and coconut husk-CHB and two copper concentrations (0.17 mg kg−1-CLS soil; 100 mg kg−1- CTS soil) on plant growth, copper uptake, and physiological response of Brassica juncea. The low- and high-Cu soils were also tested without biochar. We evaluated plant biomass, plant Cu, N and P, chlorophyll content, and chlorophyll's transient fluorescence. Plant growth was meager without biochar, indicating that the high Cu concentration was not the only limiting factor. Biochar (OBB and CHB) increased shoot mass by 300–574% and root mass by 50–2900%, and improved chlorophyll content and photosynthetic activity by 6–16%. Both biochars were efficient in the low-Cu soil as they increased plant biomass, shoot copper concentration, and translocation factor. In the high-Cu soil, both biochars increased plant biomass and copper uptake and reduced shoot copper concentration and translocation factor. The CHB and OBB removed 342% and 783% more Cu from the contaminated soil than the Control; therefore, the OBB was proven to be the best choice for phytoremediation. Novelty statement Our study showed that the orange bagasse biochar can be successfully applied for the phytoremediation of copper-contaminated soils using Brassica juncea. The orange bagasse biochar was effective regardless of the copper level in the soil, removing twice as much copper as the coconut biochar; therefore, it can speed up the process and reduce the time needed to clean up the site. Biochar significantly improved the plant's physiological response Biochar increased plant growth and copper uptake in the contaminated soil Translocation factor was increased in the clean soil and reduced in the contaminated soil Biochar from orange bagasse is more effective than coconut husk for phytoremediation [ABSTRACT FROM AUTHOR]

The aim of the research is the dissipation of Phenanthrene in sodic soil in the presence of PGPR by two mucilagenous plant species (Trigonella foenum-graceum and Brassica juncea). To evaluate of affectivity of bioremediation in the presence of local sodic soil was artificially contaminated with the known different concentration of Phenanthrene separately to analyze the dissipation of Phenanthrene. Soil samples were taken after the 30 days of incubation in the presence of plant species and PGPR. Gas Chromatography result clearly indicate that Trigonella foenum-graceum (99%) is more efficient plant over the Brassica juncea (96%) is further indicate that PGPR having synergistic effect on phytoremediation of Phenanthrene. Trigonella foenum-graceum is more efficient plant species over the Brassica juncea to dissipate Phenanthrene contaminated sodic soil. PGPR play synergistic effect on phytoremediation. [ABSTRACT FROM AUTHOR]

The effects of sunn hemp and Mexican sunflower extracts were evaluated on the development of Meloidogyne incognita on two vegetables, Amaranthus cruentus and Solanum macrocarpon in the screenhouse in 2017. Seedlings of each vegetable were inoculated with 3000 eggs of M. incognita per pot. There were five treatments; sunn hemp and Mexican sunflower extracts at 20 and 40 g leaf tissues/L water for each and a non-treated control. The treatments were laid out in a randomized complete block design in four replications. Data were collected on nematode counts, egg masses, developmental stages and gall-index. Extracts of sunn hemp and Mexican sunflower at 40 g leaf tissues/L water, significantly reduce nematode number, egg mass and gall-index in comparison with plants treated at 20 g leaf tissue/L water and non-treated control plants. Plants treated at lower rate were not different from the non-treated control plants in all parameters measured. The results showed that sunn hemp or Mexican sunflower at 40 g leaf tissue/L could be used in managing nematode pest. [ABSTRACT FROM AUTHOR]

Plant productivity relies on nitrogen (N) fertilization. However, excessive use of N-fertilizers in agriculture is detrimental to soil quality, environment and health. Hence, development of novel approaches for enhanced agricultural productivity while sustaining the environmental quality is indispensable. Agricultural sustainability with improved plant yield can be attained by employing nutrient formulation (NF) containing sub-optimal level of N along with plant growth promoting rhizobacteria (PGPR). Present study describes the development of NFs containing PGPR (Cedecea davisae RS3 and Klebsiella pneumoniae RS26) and varying N levels, by general and central composite rotatable design (CCRD) based response surface methodology (RSM) approaches and effect of their application on growth and yield of mustard plant. In general approach, plant application of NF supplemented with PGPR and sub-optimum level of N (0.5 mM) resulted in highest seed yield (5.76 g plant−1). Further CCRD based RSM optimization of PGPR and N dosages and their application on plants enhanced the seed yield to 8.20 g plant−1. The results suggest that RSM based optimization of NF could be beneficial for enhanced plant growth and yield leading to agricultural sustainability. [ABSTRACT FROM AUTHOR]

Biological and behavioural characteristics of Diaeretiella rapae were studied in a laboratory using Lipaphis erysimi as a host. The total developmental period of the parasitoid was longer in early instars as compared to that in later instars. Host age at the time of oviposition affected the oviposition period, post-oviposition period and fecundity of the parasitoid. Survival of the immatures was higher in older than the younger instars. The sex ratio for the parasitoid was found to be female-biased. Adult longevity of D. rapae was longer for females when compared to that of males. The mean longevity of adult females of the parasitoid was longer while parasitizing nymphs of younger age groups (1–2 days) compared to that of older age groups (4–5 days). L. erysimi reared on mustard was found to be the best host for D. rapae based on high parasitisation (%), female-biased sex ratio and the shortest time required by the parasitoid for completion of the total developmental period. The parasitoid showed a higher preference towards second instar nymphs of L. erysimi, both in choice and no-choice tests. The per capita parasitisation and search efficiency of the parasitoid decreased with an increase in parasitoid density. Logistic regression exhibited type-II functional response for the parasitoid D. rapae. Overall, D. rapae appeared to be a potential biocontrol agent of L. erysimi that would supplement its biological control in oilseed brassicas. [ABSTRACT FROM AUTHOR]

Selenium (Se), a metalloid typically natural in origin, is also present in coal washery by-products such as fly-ash stockpiles. The removal of Se in coal washery by-products can be achieved through various bio-physico-chemical processes. This study investigated the phytoremediation of Se from post coal process wastes using Brassica juncea species. The selected plant species were grown in coal process wastes enriched with either a growth soil mix or hydroponic substrates. Successful Se extraction (48% and 28%) was achieved from both mixes. The tested plant species also accumulated other heavy metals (Arsenic, Cadmium, Copper, Lead) along with selenium in the plant biomass. [ABSTRACT FROM AUTHOR]

The SQUAMOSA promoter binding protein (SBP)-box consists of a family of transcription factors unique to plants and mediates important roles in plant growth and development. We identified 57 SBP genes in the Brassica juncea (swollen-stem mustard) genome, and analyzed the structural characteristics, evolutionary relationships, as well as their involvement in regulation and response to environmental stress. By the help of phylogenetic analysis, the SBP genes were divided into seven subgroups (G1-G7). Interestingly, most members of the same group shared conserved gene structure and motifs as well as gene duplications. This suggests that their functions are redundant highlighting evolutionary relatedness. Identification of the cis-acting elements of the promoter revealed multiple regulatory elements that responded to phytohormone or abiotic stresses. In addition, data from GO annotation indicated that BjuSBPs play a role in biological processes. Further interrogation of the expression of BjuSBPs in different stages of swollen-stem mustard showed spatiotemporal expression patterns. On the other hand, quantitative real-time polymerase chain reaction (qRT-PCR) showed that the expression levels of SBP genes at the development stage correlated with their transcriptome data but treatment with phytohormones affected their expression levels. Our study showed that the SBP-box responds to phytohormone signals and the genes might confer different functions in the development of the mustard plant. Our data lay the foundation for further studies evaluating the regulatory mechanisms of SBP-box family of genes. [ABSTRACT FROM AUTHOR]

Selecting (inter-varietal) Brassica juncea for tolerance to metal-contamination has been proposed as a strategy to develop superior genotypes for phytoextraction of lead (Pb) through selection and breeding techniques. To understand the differences among accessions of a single species to Pb accumulation, a pot experiment was conducted with three B. juncea accessions under levels of Pb added to the soil (0, 90, 180, and 540 mg kg−1). The duration of the growth period was 100 d. Pb concentration levels did not affect the flowering of B. juncea accessions. Plant height, shoot dry matter, and root dry matter were reduced linearly when soil Pb concentration increased to 540 mg kg−1. A significant interaction between Pb concentration levels and accessions was observed for Pb concentration in shoots and roots, indicating genotypic variation in Pb absorption. The concentration of Pb in shoots in accession PI 180266 was 51% higher compared to accessions PI 649105 and PI 432379 when soil Pb concentration increased to 540 mg kg−1. It can be concluded that the B. juncea accessions differed significantly in Pb uptake, and the selection of tolerant cultivars might be helpful for Pb phytoremediation of contaminated soils. [ABSTRACT FROM AUTHOR]

Extraction of oil from wild mustard seeds with a yield of 34.0% w/w, followed by transforming it to biodiesel fuel via KOH-catalyzed transesterification with methanol assisted by a co-solvent with applying dry washing for purifying the as-produced methylic esters using the activated carbon which was prepared from mustard de-oiled residue was the major target of this investigation. Impact of reaction parameters on co-solvent transesterification of wild mustard oil (WMO) with methanol, like the molar ratio of methanol/WMO, amount and kind of co-solvent, the KOH amount, co-solvent/methanol % v/v, temperature and the duration, were inspected. The excellent conditions that resulted in the production of maximum biodiesel yield (97.88 ± 1.50%, 96.89 ± 1.0%purity) was accomplished using 6:1 methanol/WMO molar ratio, 1:1 hexane/methanol (% v/v), 0.80% KOH w/w, a temperature of 50°C for 45 minutes and (4.0% w/w) of the activated carbon for purifying the obtained unpurified methylic esters. Properties of the as-produced biodiesel were analyzed in accordance with ASTM D6751 typical test methods and found fulfilled the biodiesel typical criteria. The 1H NMR and FTIR spectroscopy analysis guaranteed transformation of the WMO to methylic esters. It was concluded that co-solvent methanolysis is more effective, time saving and helpful as it could lessen the optimal conditions necessary for attaining the conversion compared with the usual process. [ABSTRACT FROM AUTHOR]

Selenium (Se) is an essential micronutrient for humans but is toxic when consumed in excess through the food chain, such as vegetables. Therefore, it is imperative to understand the relationship between the bioavailability of Se in soil and its uptake in edible parts of vegetables. This study investigated Se bioavailability of Brassica juncea in six representative Chinese soils treated with different concentrations of exogenous selenate fertilizer (0–2 mg·kg−1) by comparing diffusive gradients in thin-films (DGT) and chemical extraction methods. The correlation coefficients between the Se uptake by Brassica juncea and soil available Se determined by different extraction methods was as follows: DGT > KCl > Water > EDTA > KH2PO4 > NaHCO3 extractions. In addition, soil properties were correlated between Brassica juncea and soil Se concentrations determined by chemical extraction methods, while the DGT method was independent of soil properties. DGT was more suitable for the measurement of Se thresholds for Udic Ferrisols, Mollisols, Stagnic Anthrosols, Fluviogenic Loamy Paddy soil, Silty Loam soil, and Calcaric Regosols with values of 373.34, 648.68, 436.29, 589.84, 295.35, and 464.95 μg·L−1, respectively. Thus, DGT may be an effective method for the prediction and evaluation of Se bioavailability to Brassica juncea in different soil types. [ABSTRACT FROM AUTHOR]

A Phytoremediation experimental set up was established by spiking the soil with varying concentrations of mercury (Hg) (Treatment: T1:10; T2:50; T3:100; T4:500 and T5:1,000 mg Hg/kg soil). Hg removal ability of the Indian mustard plant was determined after 30, 60 and 90 days of exposure. Hg accumulation trend in second and third month of exposure was root > leaf > stem, while for the 1st month it was root > stem > leaf. The highest percentage of Hg accumulation (81%) and glutathione (14 mg/kg) was observed in the plants of T4 and T5 treatment, respectively at 90 days of exposure indicating a high level of Hg stress tolerance. At 90 days of exposure the chlorophyll a content in leaves grown on Hg-free soil (control soil) was 1.8, 2.4, 2.8, 3.6 and 4.4 fold higher than T1, T2, T3, T4 and T5 treatment respectively. With increase in exposure duration, translocation factor decreased whereas bioconcentration factor increased signifying Hg is mainly accumulated in the roots. The study suggests that Brassica juncea can withstand under high Hg contamination and can show great potential to phytostabilize Hg when grown under 100 mg/kg of soil Hg without showing any significant detrimental effect on the plant. [ABSTRACT FROM AUTHOR]

Lipaphis erysimi Kaltenbach is one of the economically important pests of Indian mustard Brassica juncea. Plant defense against these pests include impairing digestive processes in the insect gut. Thus, for an effective pest management, it is imperative to understand the digestive enzymes present in the pest. The present study involves the characterization of mustard aphid α-amylase and the efficacy of a potent insecticidal agent, RiD (Rorippa indica defensin) against it. The total protein of L. erysimi was analysed to determine the α-amylase activity present in it. The relative α-amylase activity in the total protein was assayed by the dinitrosalicylic acid (DNS) procedure, using 1% soluble starch as substrate and was observed to be 2.32 ± 0.025 U/mL of the total protein. The optimum temperature for α-amylase activity was found to be 35 °C and optimum pH was recorded as 6.5. Presence of RiD showed a decrease in the α-amylase activity in a non-competitive way. Moreover, cryo-sections of the aphid midgut also revealed bound RiD in the confocal analysis. [ABSTRACT FROM AUTHOR]

Flowering time is an important agricultural trait of the oil crop Brassica juncea (B. juncea), as accelerated flowering enables avoidance of terminal drought leading to increased yields. One gene known to control flowering time is TERMINAL FLOWER 1 (TFL1), which belongs to a family of phosphatidylethanolamine binding proteins, which can either repress or promote flowering time. Here, a TFL1 homolog, named BjTFL1, has been isolated from B. juncea, which shared 95% amino acid identity with TFL1 from Arabidopsis thaliana. Sequence analysis predicts the BjTFL1 protein contains the ligand-binding site, conserved motifs and other amino acid residues that are critical for TFL1 function. Confirming this as a functional TFL1 orthologue, overexpression of BjTFL1 under the control of the constitutive 35S promoter in Arabidopsis delayed flowering time. As a proof-of-concept to investigate its utility to shorten flowering time, an RNAi construct containing a partial sequence of BjTFL1 was transformed into Arabidopsis. Transcript analysis demonstrated the downregulation of endogenous AtTFL1. Moreover, the RNAi BjTFL1 transgenic lines were early flowering and had fewer rosette and cauline leaves compared to wild-type. Therefore, this BjTFL1 RNAi transgene could be used as a biotechnological tool to reduce flowering time in Brassica juncea in a bid to improve agricultural performance. [ABSTRACT FROM AUTHOR]

In this study, different soil Pb concentrations [24 (control), 80, 136, 362, and 1150 mg kg−1] were used to analyze the tolerance threshold and accumulation potential of Brassica juncea L. in a pot experiment under greenhouse conditions. In addition to growth and Pb accumulation, the following contamination indices were calculated: transfer coefficient (TC), translocation factor (TF), and tolerance index (TI). Growth and Pb accumulation were determined at 60 days after emergence. The Pb concentrations were determined using the flame atomic absorption spectrometry (FAAS). The plant height was affected by soil Pb contamination, and it decreased from 1.37 to 0.83 m when the soil Pb concentration increased from 24 (control) to 1150 mg kg−1, respectively. The Pb concentration in the shoots and roots increased as the Pb concentration in the soil increased, reaching 94 mg kg−1 in shoots and 783 mg kg−1 in roots when was grown under 1150 mg kg−1 of Pb. TF was <1 at all levels of contamination. The TI values suggested that B. juncea presented Pb tolerance in Pb contaminated soils. Our findings indicate that B. juncea has the potential to accumulate Pb in soil under tropical conditions. [ABSTRACT FROM AUTHOR]

A field experiment was conducted to evaluate the effect of sulfur fertilization and irrigation scheduling on mustard hybrids in Varanasi, India. Experiment comprised 18 treatment combination involving three irrigation scheduling (0.4, 0.6 and 0.8 IW/CPE) and two hybrids (NRCHB-506 and PAC 432) as main plot treatment and three sulfur (S) levels (0, 30 and 60 kg S ha−1) as sub-plot treatment in split-plot design replicated thrice during Rabi season (Oct–March) of 2015–16 and 2016–17. Statistical analysis of the results revealed that individually irrigation scheduling at 0.8 IW/CPE, mustard variety 'PAC 432ʹ and sulfur application at 60 kg ha−1 reported to have maximum plant height, number of primary and secondary branches, dry matter accumulation, number of siliquae plant−1, number of seeds siliqua−1, siliqua length and seed yield. Similar results were also obtained in relation to dry matter heat use efficiency (DM-HUE) at different stages and seed yield HUE. However, the variation in most of the parameters observed with either 0.6 or 0.8 IW/CPE and application of 30 or 60 kg S ha−1 was found non-significant during the course of the trial. In terms of interaction, mustard variety 'PAC 432ʹ irrigated at 0.8 and 0.6 IW/CPE and fertilized with 60 or 30 kg S ha−1 proved significantly superior over other treatments and recorded the highest plant height, better yield constituents and maximum yield, while the lowest values for the same were recorded in 'NRCHB-506ʹ irrigated at 0.4 IW/CPE with no sulfur application during both the years of experimentation. [ABSTRACT FROM AUTHOR]

Soilborne diseases are the most economically important problem for ornamental nursery producers in the southeastern United States. The use of cover crops selected based on their biofumigant activity to improve soilborne disease management in woody ornamental production was assessed. Replicated pot bioassays were established as greenhouse trials in sterilized clay loam soil which had pre-existing populations of Rhizoctonia solani or Phytophthora nicotianae. Selected Brassica crops were seeded directly into the soil and flowering cover crops were incorporated 15 cm deep into the same pots and covered with polyethylene for 2 or 4 weeks. Volatile compounds released during the biofumigation process were collected at different time intervals. Soil type and moisture affected ITC release. Hydrangea or viburnum rooted cuttings were grown in the biofumigated (2 or 4 weeks) and non-biofumigated control pots and root rot disease severity was evaluated at the end of each bioassay. Yellow mustard (Sinapis alba, 'White gold'), turnip (Brassica rapa, 'Purple top forage'), arugula (Eruca vesicaria ssp. sativa, 'Astro'), Mighty mustard (B. juncea, 'Pacific gold'), rape (B. napus, 'Dwarf essex'), mustard green (B. carinata, 'Amara') and brown mustard (B. juncea, 'Kodiak') cover crops were effective in suppressing R. solani and P. nicotianae. Similar disease suppression was observed whether biofumigation was performed for 2 or 4 weeks. Phytotoxicity was not observed on viburnum and hydrangea woody ornamental plants after either the 2 or 4 weeks biofumigation period with any of the tested cover crops. Viburnum and hydrangea grown in mustard green-, arugula- and turnip-incorporated soil had significantly higher whole plant and root fresh weights compared with the inoculated, non-biofumigated control plants. Although mustard green and arugula are not used currently as commercial biofumigation cover crops, they also showed promise for controlling soilborne pathogens of woody ornamental plants under greenhouse conditions. [ABSTRACT FROM AUTHOR]

A pot experiment (Net House) study was conducted in the Department of Soil Science and Agriculture Chemistry, Institute of Agricultural Science, B.H.U. Varanasi to find out the effect of organic amendments in mustard grown in chromium (Cr)-contaminated soil during 2015–16 and 2016–17. There were five levels of Cr (Control, 20, 40, 60 and 80 mg/Kg soil) with and without three organic amendments (viz. Vermicompost (VC), FYM, and Sewage Sludge (Sl)) @ 5 t/ha, 10 t/ha and 20 t/ha, respectively. The maximum number of siliqua/plant (333 and 354), length of siliqua (5.43 and 5.53), number of seeds per siliqua (12.11 and 12.39), dry matter accumulation (50.76 and 54.65g/pot), biological yield (266.51 and 273.34g/pot), seed yield (62.63 and 66.96g/pot), stover yield (203.88 and 206.38g/pot), and harvest index (23.50 and 24.5%) were recorded when Cr-contaminated soil is treated with organic amendments mainly with vermin compost during 2015–16 and 2016–17. It is reported that significant nutrient uptake (N, P, S, K, Fe, Cu, Zn, and Mn) also increased with the application of organic amendments. It is reported that yield attributing characters are improved by the application of VC followed by farm yard manure then sewage Sl. [ABSTRACT FROM AUTHOR]

In the present study, ecological control of P. xylostella was examined in two steps, primary (using trap cropping) and supplementary (using Bt applications). Perimeter trap cropping was applied at 500-m2 plots of cauliflower using trap crops (Indian, field and white mustards, Chinese cabbage, sweet alyssum, pot marigold). Random sampling was performed to estimate the populations of P. xylostella adults on trap crops, P. xylostella pre-adults on main crops, percentage parasitism, damage intensity and crop yield. There was significant differences between treatments for adults, pre-adults, preference and reduction indices, linear correlation and regression between pest attraction to trap crop and pest population reduction on main crop, percentage parasitism and damage intensity, but not for crop yield. The minimum Bt application was happened on Indian mustard treatment (two times). The present study showed that it is practical to ecologically control the diamondback moth using a combination of trap cropping and Bt spray. [ABSTRACT FROM AUTHOR]

Identifying genotypes of high nitrogen utilization efficiency (NUtE) under contrasting N supply is a way of improving N economy of Indian mustard (Brassica juncea Czern & Coss) under different soil fertility. A field experiment was conducted with 22 Indian mustard genotypes grown under normal and limited N supply conditions to investigate the genotypic variations of NUtE. The genotypes ranking to NUtE was different under normal and limited N supply, however, seven genotypes (RH 1222-28, RH 1301, RH 1117, RH 601, RH 1053, NRCDR 2, RH 1441) were identified as N-responder under normal N supply, and N-efficient under limited N supply. The lowest seed yield reduction (<20%) under limited N supply was recorded in RH 601 (15%), RH 1301 (17%), RH 1215 (17%), and DRMRIJ 31 (18%) compared to normal N supply. Among these genotypes, DRMRIJ 31 and RH 1215 were the highest seed yielder (>3.0 t/ha) under normal N supply and RH 601 (>2 t/ha) under limited N supply. The linear regression analysis showed significant and positive relationship between N uptake and seed yield. Whereas, N uptake and utilization efficiency showed negative trend and decreased with increase in N uptake beyond 120 kg N/ha under normal N supply, and 60 kg N/ha under limited N supply. NUtE was positively correlated with seed yield under both normal as well as limited N supply. These results may be used to identify the key associated traits, and to isolate target genes for developing varieties/hybrids to harness better N economy. [ABSTRACT FROM AUTHOR]

Electrokinetic-enhanced phytoremediation is an effective technology to decontaminate heavy metal contaminated soil. In this study, we examined the effects of electrokinetic treatments on plant uptake and bioaccumulation of U from soils with various U sources. Redistribution of uranium in soils as affected by planting and electrokinetic treatments was investigated. The soil was spiked with 100 mg kg–1 UO2, UO3, and UO2(NO3)2. After sunflower and Indian mustard grew for 60 days, 1 voltage of direct-current was applied across the soils for 9 days. The results indicated that U uptake in both plants were significantly enhanced by electrokinetic treatments from soil with UO3 and UO2(NO3)2. U was more accumulated in roots than in shoots. Electrokinetic treatments were effective on lowering soil pH near the anode region. Overall, uranium (U) removal efficiency reached 3.4–4.3% from soils with UO3 and uranyl with both plants while that from soil with UO2 was 0.7–0.8%. Electrokinetic remediation treatment significantly enhanced the U removal efficiency (5–6%) from soils with UO3 and uranyl but it was 0.8–1.3% from soil with UO2, indicating significant effects of U species and electrokinetic enhancement on U bioaccumulation. This study implies the potential feasibility of electrokinetic-enhanced phytoremediation of U soils with sunflower and Indian mustard. [ABSTRACT FROM AUTHOR]

In this paper, Ba 0. 2 Sr 0. 8 deposition method with spin coating technique in order to improve its dielectric properties. This study was to find out how the effect of mustard chlorophyll exposure on Ba 0. 2 Sr 0. 8 TiO 3 films was analyzed using x-ray diffraction and micro-Raman spectroscopy based on crystal structure and the stability of the film phase BST doping mustard chlorophyll. The annealing process is carried out as a high temperature heating step which aims to restore the physical and crystalline properties of the film which may change during the film growth process. The specified annealing temperature is 850 °C with a holding time of 8 h. The effect of chlorophyll solidification was observed by applying variations of chlorophyll mustard concentration, namely 0%, 2.5%, and 5%. The crystal structure type and film lattice parameters were confirmed using X-Ray Diffraction with the measurement angle starting from an angle of 10° to 80° with an angle increase of 0.02°. [ABSTRACT FROM AUTHOR]

Landfill gases are produced due to biodegradation of organic fraction of municipal solid waste (MSW) when water comes into contact with buried wastes under prevailing anaerobic conditions. To minimize the percolation of water in landfills, the conventional approaches such as clay capping is still practised in India. As landfill gas extraction system is an expensive technique,'Phytocapping' to mitigate landfill gases and to minimize percolation of water into the landfill can be one of the attractive alternatives. The present study aimed to determine efficiency of a laboratory-scale phytocap in terms of methane emissions, heavy metals' remediation and plant–soil interactions in Indian climatic conditions using six native plants in five planters (30 cm × 30 cm × 25 cm) with 5 kg of MSW. Overall methane oxidation due to vegetation was observed throughout the vegetated planters when compared to non-vegetated planters. Root zone methane concentrations were also monitored for the plant species, with the highest reduction occurring in root zones of Agave sisalana and Bambusa sulfurea and highest trace elements' remediation potential was observed in Brassica juncea and Helianthus annus plants. 96–98% of CH4 oxidation and 85–89% of heavy metals remediation was achieved through the study. [ABSTRACT FROM AUTHOR]