1. CNA-loaded PLGA nanoparticles improve humoral response against S. aureus -mediated infections in a mouse model: subcutaneous vs. nasal administration strategy.
- Author
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Genta, Ida, Colonna, Claudia, Conti, Bice, Caliceti, Paolo, Salmaso, Stefano, Speziale, Pietro, Pietrocola, Giampiero, Chiesa, Enrica, Modena, Tiziana, and Dorati, Rossella
- Subjects
COLLAGEN-binding proteins ,STAPHYLOCOCCUS aureus infections ,MICE ,POLYLACTIC acid ,NANOPARTICLES ,SUBCUTANEOUS infusions ,INTRANASAL medication ,THERAPEUTICS - Abstract
The aim of this work was the assessment of the “in vivo” immune response of a poly(lactide-co-glycolide)-based nanoparticulate adjuvant for a sub-unit vaccine, namely, a purified recombinant collagen-binding bacterial adhesion fragment (CNA19), againstStaphylococcus aureus-mediated infections. “In vivo” immunogenicity studies were performed on mice: immunisation protocols encompassed subcutaneous and intranasal administration of CNA19 formulated as nanoparticles (NPs) and furthermore, CNA19-loaded NPs formulated in a set-up thermosetting chitosan-β-glycerolphosphate (chitosan-β-GP) solution for intranasal route in order to extend antigen exposure to nasal mucosa. CNA19 loaded NPs (mean size of about 195 nm, 9.04 ± 0.37μg/mg as CNA19 loading capacity) confirmed as suitable vaccine for subcutaneous administration with a more pronounced adjuvant effect (about 3-fold higher) with respect to aluminium, recognised as “reference” adjuvant. CNA19 loaded NPs formulated in an optimised thermogelling chitosan-β-GP solution showed promising results for eliciting an effective humoral response and a good chance as intranasal boosting dose. [ABSTRACT FROM PUBLISHER]
- Published
- 2016
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