1. Separation and nanoencapsulation of antitumor peptides from Chinese three-striped box turtle ( Cuora trifasciata ).
- Author
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He, Shengjie, Mao, Xinliang, Zhang, Ting, Guo, Xiaolei, Ge, Yazhong, Ma, Chungwah, and Zhang, Xuewu
- Subjects
PEPTIDES ,TURTLES ,TRYPSIN ,CANCER cells ,COMPUTER simulation - Abstract
Chinese three-striped box turtle (Cuora trifasciata), as a freshwater turtle, is used as a tonic food. The purpose of this study was to isolate peptides with cancer growth inhibition activity from trypsin-digested hydrolysates of turtle proteins. The results demonstrated that two fractions T1 and T2 exhibited good inhibition on HepG-2 and MCF-7 cancer cells, with an inhibition of 70.65–89.1%, at 500 μg/mL. Subsequently, three peptides were identified from T1 and T2, including RGVKGPR (T1–1), KLGPKGPR (T1–2), and SSPGPPVH (T2–1). By database search, T2–1 was a completely new peptide; its inhibition activity on MCF-7 cancer cells was the best, up to 70.02% at 500 μg/mL. Then, T1 and T2–1 were nanoencapsulated by chitosan. After nanoencapsulation, the inhibition percentages were 50.23% for the nanoencapsulated T1 on HepG-2 and 46.82% for the encapsulated T2–1 on MCF-7. The release experiment indicated that the encapsulated peptides could be slowly released in simulated gastrointestinal juice. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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