1. Preparation, Identfication, and Activity Assay of Lamprey ( Lampetra japonica ) Natural Intelectins.
- Author
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Su, Peng, Zheng, Zhen, Pang, Yue, Xue, Zhuang, Gou, Meng, Han, Yinglun, Liu, Ge, Zan, Qi, and Li, Qingwei
- Subjects
LAMPREYS ,NATURAL immunity ,IMMUNE response ,LAMPETRA ,AGAROSE ,POLYACRYLAMIDE gel electrophoresis - Abstract
Intelectins play an important role in innate immune response. In a previous study, lamprey inteletins purified by galactose-Sepharose were inactive and insoluble. Herein, we provided a simple and effective method to purify natural intelectins from the serum of lamprey (Lethenteron japonicum) using proteinG agarose. SDS-PAGE, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and mass spectrometry (MS) were used to analyze the purified proteins. The purified proteins were identified to be lamprey serum lectin and intelectinB. The activity analysis results indicated that the proteins had certain extent agglutination activity. The effective method will be useful to study their immune functions and molecular mechanisms. [ABSTRACT FROM PUBLISHER]
- Published
- 2015
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