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AbstractIn the short-season winter environment of India and Bangladesh, lentil growth and seed yield are significantly hindered by foliar blight caused by Stemphylium botryosum. As the international germplasm pool lacks a resistance source, the study aims to develop a mutant population to identify a high-yielding mutant resistance against the pathogen. A gamma-irradiated population was developed based on its GR50 dose of 248.8 Gy. The screening of almost 130,000 M2 plants identified a tolerant lentil mutant, MM216. The multi-location trials revealed that MM216 showed an impressive and robust resistance; the selected mutant line could be recommended as a donor in the lentil breeding program against the pathogen globally. A 100 g seed was exposed to a GR50 dose to develop the M1 population. At maturity, at least 100 M2 seeds of each 1300 M1 plant were harvested individually. So, almost 130,000 M2 plants were screened in the disease hot spot. The selected mutants were advanced to M7 by screening in the field and challenged in controlled conditions with the pure pathogen isolate. A resistance mutant, MM216, with a per cent disease index (PDI) of <10, was identified where the mean of the check varieties, WBL 77, was >55. The resistance ability was confirmed further in controlled conditions. The fungal and plant DNA ratio was almost negligible in the tolerant mutant, whereas it was 0.17 in WBL77 at 196 h post-inoculation. The selected mutant did not display any yield penalty, but there was a delay in flowering by a week compared to WBL77. [ABSTRACT FROM AUTHOR]

Lentils (Lens culinaris Medik.), the earliest legumes grown by humans, are essential food sources in many countries. This review summarizes the nutrient profile, phytochemical profile, sensory properties, bioaccessibility, along with health benefits of lentils. Lentils are not only rich in protein, carbohydrates, and fats but various bioactive substances, especially flavonoids and phenolic acids. However, their bioavailability is poor due to the presence of certain antinutritional factors, containing tannins, phytic acid, protease inhibitors as well as lectins. Specific information for the bioaccessibility and bioavailability of phenolics and minerals in lentils is listed. Besides, several conventional along with novel techniques that used to improve bioaccessibility and bioavailability of lentils, such as soaking, dehulling, thermal processing, isoelectric precipitation, pulsed electric field, and high-pressure processing are introduced as well. In addition, various potential health benefits are reviewed, including anti-inflammatory, anticancer, antidiabetic, and anti-obesity properties. Thereby, increased consumption and utilization of lentils as functional foods are considered the key to combating certain chronic diseases in the human body. Lastly, some suggestions are put forward for the future direction of lentils. [ABSTRACT FROM AUTHOR]

The study aimed at examining the effect of Iron (Fe) management strategies on the redistribution of Fe fractions in soil and on the Fe bioavailability and phytic acid (PA) content in lentil grains. A pot experiment was conducted in Completely Randomized Design with 8 treatments replicated thrice. The treatments were Control (no fertilizer); Recommended dose of fertilizers (RDF) i.e. 20:40 (N: P2O5); RDF + Fe (basal application @10 kg/ha); RDF + Fe (foliar@ 1% (w/v) solution); RDF + Fe (basal + foliar); RDF + FeSB (biopriming with Fe solubilizing bacteria (Enterobacter cloacae strain BAU3) @ 5 mL/kg seed (1 x109 cfu mL−1)); RDF + Fe (seed nutripriming with 1% (w/v) solution); and RDF + (biopriming + nutripriming). Fe source for the treatments was Fe-EDDHA chelate. Higher Fe release from these fractions was observed under biopriming + nutripriming with water soluble Fe of 21.09 and 20.82 mg kg−1, respectively for the biopriming + nutripriming and sole biopriming treatments; and organically bound Fe of 230.95 and 211.36 mg kg−1 for the same treatments. These fractions contributed toward higher Fe uptake which was highest under biopriming + nutripriming. Values were statistically significant for biopriming and nutripriming and basal + foliar treatment. Lowest PA values in lentil grains as well as PA/Fe ratio were estimated under basal + foliar and biopriming + nutripriming treatments. Combined basal and foliar application of Fe esp. in chelated form and use of seed priming strategies have immense potential in enhancing the Fe bioavailability in grains from soil, seed or foliage. [ABSTRACT FROM AUTHOR]

Because reactive oxygen species are involved in the regulation of biological rhythms, we hypothesized that intra-annual variability of seed progeny quality at low doses of ionizing radiation (LDIRs) would differ from that of background plants. We conducted 12 consecutive experiments using the roll culture method by germinating seeds (monthly for 3 weeks) of six herbaceous plant species (Bromus inermis, Geum aleppicum, Plantago major, Rumex confertus, Silene latifolia, and Taraxacum officinale) growing under conditions of chronic radiation in the East Ural Radioactive Trace (EURT). We assessed physiological (seed viability and abnormality frequency) and biochemical (low-molecular-weight antioxidants, LMWAs) parameters of seedlings. Total absorbed dose rates of maternal plants (TADRplants) and seed embryos (TADRseeds) in the EURT exceeded background levels by 1–3 and 1–2 orders of magnitude, respectively. Nonlinear dependencies on TADR were mainly characteristic of physiological and biochemical parameters. For most populations of the studied species (B. inermis, G. aleppicum, R. confertus, and S. latifolia), seedling survival and root length decreased in the autumn–winter period, while the frequency of abnormal seedlings increased. The content of LMWAs could be ranked as R. confertus > B. inermis > G. aleppicum > S. latifolia, in good agreement with the presence of anthocyanin pigmentation in the plants. The lowest synthesis of antioxidants in seedlings was observed in winter. A high LMWA content promoted growth and reduced the frequency of abnormal seedlings. These results underscore a multistage nature of the impact of LDIRs on intra-annual biological rhythms in plants. High heterogeneity in reference group 'wild grasses' and diversity of their radiobiological effects should help to develop methods of radiation protection for natural ecosystems and facilitate approaches used by the International Commission on Radiological Protection. Absorbed dose rates for six plant species in the East Ural Radioactive Trace (EURT) area range from 0.11 to 73.89 µGy h−s (plants) and 0.11 to 6.88 µGy h−s (seed embryos). Intra-annual rhythms of physiological and biochemical parameters in the EURT zone differ from those in background seedlings. Plants in the EURT area exhibit a wide range of trait variability, asynchrony of the manifestation of the effects, nonlinear dose–response relations, and hormesis. A high content of low-molecular-weight antioxidants (LMWAs) is associated with low frequency of developmental abnormalities and high viability of seed progeny. [ABSTRACT FROM AUTHOR]

To provide an updated summary of recent advances in the application of gamma irradiation to elicit secondary metabolism and for induction of mutations in plant cell and organ cultures for the production of industrially important specialized metabolites (SMs). Research on the application of gamma radiation with plants has contributed a lot to microbial decontamination of seeds, and the promotion of physiological processes such as seed germination, seedling vigor, plant growth, and development. Various studies have demonstrated the influence of gamma rays on the morphology, physiology, and biochemistry of plants. Recent research efforts have also shown that low-dose gamma (5–100 Gy) irradiation can be utilized as an expedient solution to alleviate the deleterious effect of abiotic stresses and to obtain better yields of plants. Inducing mutagenesis using gamma irradiation has also evolved as a better option for inducing genetic variability in crops, vegetables, medicinal and ornamentals for their genetic improvement. Plant SMs are gaining increasing importance as pharmaceutical, therapeutic, cosmetic, and agricultural products. Plant cell, tissue, and organ cultures represent an attractive alternative to conventional methods of procuring useful SMs. Among the varied approaches the elicitor-induced in vitro culture techniques are considered an efficient tool for studying and improving the production of SMs. This review focuses on the utilization of low-dose gamma irradiation in the production of high-value SMs such as phenolics, terpenoids, and alkaloids. Furthermore, we present varied successful examples of gamma-ray-induced mutations in the production of SMs. [ABSTRACT FROM AUTHOR]

Pulses are edible seeds belonging to the Leguminosae family for human consumption and consist of various species such as common beans (Phaseolus vulgaris L.), peas (Pisum sativum), lentils (Lens culinaris), chickpeas (Cicer arietinum L.), and faba beans (Vicia faba). Pulses are sustainable sources of nutritional compounds, especially containing almost twice the protein content compared to cereal grains. In addition to becoming an excellent source of macronutrients and micronutrients, they are abundant in phytochemicals, containing bioactive compounds with potential health benefits resulting from various phenolic compounds, as well as antinutritional compounds (e.g. phytic acid, enzyme inhibitors, lectins, saponins), which have received widespread concern by researchers. More essentially, various processing approaches for consumption purposes will result not only in enhanced nutritional and sensory characteristics in pulses but also in affected phytochemicals contents and their bioavailability. In this review, the nutritional and phytochemical compositions of pulses will be first introduced, followed by different common-applied processing methods (thermal and non-thermal), along with their impacts and pulse storage effects on the content of corresponding phytochemicals. Furthermore, through food processing and digestion, the bioaccessibility and bioavailability of pulse phytochemicals will be improved, thus releasing more health benefits, and expressing pharmacological functions in the human body. [ABSTRACT FROM AUTHOR]

The objective of the study was to investigate the effects of sole applications of biofertilizers and phosphorus as well as their integrated use in lentil. The field experiment was conducted to study the effect of phosphorus (0, 15, 20 and 25 kg P2O5 ha−1) and biofertilizer inoculation treatments [uninoculated control, Mesorhizobium, Mesorhizobium + RB-1 (Pseudomonas argentinensis) and Mesorhizobium + RB-2 (Bacillus aryabhattai)] on nutrient uptake, nutrient use efficiency and residual nutrient status of soil after the harvest of chickpea. Mesorhizobium + RB-1 recorded the highest uptake of nitrogen (73.7, 59.0 and 132.7 kg ha−1 in grain, straw and total, respectively), phosphorus (8.29, 11.34 and 19.63 kg ha−1 in grain, straw and total) and potassium (23.48, 43.36 and 66.84 kg ha−1 in grain, straw and total) as well as partial factor productivity (113.1 kg grain kg−1 P2O5). Application of 25 kg P2O5 ha−1 recorded the highest phosphorus concentration (0.39% in grain and 0.26% in straw), phosphorus uptake (8.35, 12.32 and 20.67 kg ha−1 in grain, straw and total, respectively) and phosphorus status in soil after crop harvest (18.7 and 16.7 kg P ha−1 in 0–15 and 15–30 cm soil layers, respectively). Partial factor productivity was recorded the highest with the application of 15 kg P2O5 ha−1 (132.2 kg grain kg−1 P2O5). It can be concluded that dual applications of biofertilizers [Mesorhizobium + RB-1 (Pseudomonas argentinensis)] and 25 kg P2O5 ha−1 improve nutrient uptake by the crop and build up of nutrients in the soil after the harvest of the crop. [ABSTRACT FROM AUTHOR]

The present investigation reports the development and characterization of a clay-polymer composite loaded with urea and Trichoderma harzianum (UTCPC) to enhance soil moisture and nitrogen availability and manage wilt-nematode complex in lentil grown in residual moisture after rainfed rice. The Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) characterization proved successful synthesis of UTCPC. The bentonite clay (8% w w−1) and Th concentration (10% w w−1) were optimized based on the maximum spore count of the UTCPC and viability of the Th spores at one month of storage at room temperature. The incubation and pot culture experiments were laid out in a completely randomized design (CRD) with six N levels replicated thrice in combinations with UTCPC. Under incubation experiment, the treatment of 100% N through farmyard manure (FYM) along with UTCPC application (T3) resulted in the highest soil moisture (18.5%) and lowest nitrate (NO3-N) content (42%) at 6 and 10 days after incubation, respectively, suggesting its sustained moisture-retaining and slow nitrate (NO3-N) release properties of the UTCPC. Under pot experiment, the treatment T3 expressed the highest soil moisture (28.8%) and NO3-N (17.6 kg ha−1) content even at 60 days after sowing. The disease severity index of all the treatments comprising UTCPC with or without nutrient application was significantly lower than the untreated soil. The lowest population of Fusarium spp. (13.8%) and Meloidogyne spp. (8%) were observed in T3. Therefore, UTCPC can be considered as an eco-friendly agri-input in enhancing moisture retention and sustaining nutrient release to the soil as well as providing biocontrol agents to manage Fusarium-Meloidogyne complex in lentil. [ABSTRACT FROM AUTHOR]

The present study investigated the effect of sole as well as combined foliar application of zinc (Zn) and urea at flowering and pod formation stages and soil application of Zn on symbiotic parameters, growth, yield, and economics of lentil. Field experiments were conducted at two locations (Ludhiana and Faridkot, northern India) during the winter season of 2018–19 and 2019–20 in randomized complete block design. The experiment comprised of 11 treatments [control (no soil/foliar application of ZnSO4 and no urea foliar application), soil Zn (25 kg ZnSO4 ha−1), foliar application of sole Zn (0.5% ZnSO4) or urea (2%) and combined Zn + urea at flowering, pod formation, or flowering + pod formation stages] and three replications. Zn and urea fertilization significantly improved the grain yield and related traits and economics in both the years of study at both the locations. The treatment of combined foliar application of Zn + urea at flowering + pod formation stages recorded the highest symbiotic parameters, chlorophyll content, growth and yield attributes, grain yield (29.5% higher than control), gross returns (29.5% higher), net economic returns (56.2% higher), and benefit cost ratio. The combined foliar application of Zn + urea can be done to improve growth, productivity, and economics of lentil and, therefore, it can help in upliftment of economic status of farmers. [ABSTRACT FROM AUTHOR]

Bronze Age agriculture in Europe is marked by the adoption of new crops, such as broomcorn millet (Panicum miliaceum), broad bean (Vicia faba) and gold-of-pleasure (Camelina sativa). Yet, at a regional level, it is sometimes unclear when, where and why these crops are adopted and whether they were all adopted at the same time. Croatia is one such region where archaeobotanical research is limited, making it difficult to discuss Bronze Age agriculture and diet in more detail. The discovery of a burnt-down house with crop stores at Kalnik-Igrišče provides a unique archaeobotanical assemblage and snapshot of late Bronze Age agriculture (1000–800 BC). From the carbonised plant remains discovered at Kalnik-Igrišče we see a dominance in the crops broomcorn millet, barley (Hordeum vulgare), free-threshing wheat (Triticum aestivum/durum/turgidum) and broad bean. Emmer (Triticum dicoccum), spelt (Triticum spelta), and lentil (Lens culinaris) were also found, suggesting they were probably minor crops, while spatial analysis indicates distinct crop storage areas within the building. Overall, these finds support the adoption and integration of these new crops within northern Croatia by the late Bronze Age, while highlighting implications for seasonal strategies, risk management, and cultural dietary choice. [ABSTRACT FROM AUTHOR]

The genus Mentha spp. is an aromatic herb from the family 'Lamiaceae'. It is extensively predominant in temperate and sub-temperate regions of the world. The essential oil of this species is enriched with broad aroma constituents extensively utilized in food, beverages, flavor, cosmetics, perfumery, and pharmaceutical enterprises. With the global menthol market size estimated to be worth USD 765 million in 2022, India (accompanied by China and Brazil) is the world's primary manufacturer, consumer, and exporter of Mentha oil. Despite prominent global demand, the crucial bottleneck in mint cultivation is the need for more superior commercial cultivars. Predominant vegetative propagation mode with difficulties in manual emasculation, differential blooming times, sterile/sub-sterile hybrids, and low seed viability are the primary containment in creating genetic variability by classical breeding approaches. Therefore, genetic complications encountered in conventional breeding have led the breeders to apply mutation breeding as an alternative crop improvement approach in Mentha spp. These attempts at mutation breeding have produced some distinctive mutants as genetic pools for plant breeding programs, and some novel mutant mint cultivars have been made available for commercial cultivation. The prime strategy in mutation-based breeding has proven an adept means of encouraging the expression of recessive genes and producing new genetic variations. The present review comprises a significant contribution of mutation breeding approaches in the development of mutant mint species and its effects on physiological variation, photosynthetic pigment, essential oil content and composition, phytochemical-mediated defense response, pathogen resistivity, and differential expression of genes related to terpenoid biogenesis. Development and diversification have led to the release of varieties, namely Todd's Mitcham, Murray Mitcham, Pranjal, Tushar, and Kukrail in M. piperita L., Mukta, and Pratik in M. cardiaca Baker, Neera in M. spicata L., Kiran in M. citrata Ehrh., and Rose mint in M. arvensis L. that have revolutionized and uplifted mint cultivation leading to economic gain by the farmers and entrepreneurs. [ABSTRACT FROM AUTHOR]

Emerging buds of Narcissus pseudonarcissus were found to accumulate the alkaloid haemanthamine (1) at high concentrations, exceeding that of narciclasine (2), the most abundant constituent in bulbs of the plant. A phytoactivity screening assay demonstrated the novel phytotoxicity of haemanthamine against Raphanus sativus (radish), Lactuca sativus (lettuce), Triticum aestivum (red wheat), Solanum lycopersicum (tomato), Cucumis sativus (cucumber), Ipomoea (Morning glory), and Lens culinaris (lentil). Haemanthamine (1) phytotoxicity was found to exceed that of the commercial herbicide glyphosate and less toxic than narciclasine (2). [ABSTRACT FROM AUTHOR]

Agronomic biofortification with iron (Fe) may be a viable approach to combat the widespread deficiency of Fe in humans. Thus, the present study was conducted to examine the effect of Fe and urea foliar application on Fe biofortification in lentil (Lens culinaris Medik.). The field experiments were conducted at two locations viz. Ludhiana and Faridkot, India. Different treatment combinations of foliar spray of sole Fe (0.5% of FeSO4.7 H2O), sole urea (2% urea), and combined application of Fe and urea at flowering, pod formation, and flowering + pod formation stages along with unsprayed control were applied to lentil. The results of the study revealed that the combined foliar application of Fe and urea at flowering + pod formation stages was the most effective in enhancing grain yield, Fe and zinc (Zn) concentration in plants (at flowering as well as pod formation stage), stover and grain (whole and split). The highest Fe concentration in whole (36% higher over control) as well as split (32.9% higher over control) grain and protein content (21% higher over control) was obtained with foliar application of Fe + urea at flowering + pod formation stages. Conclusively, the combined foliar application of 0.5% FeSO4.7 H2O and 2% urea at flowering + pod formation stages may prove to be a feasible option for increasing grain yield and enrichment of lentils with Fe and Zn to combat micronutrient malnutrition in human populations. [ABSTRACT FROM AUTHOR]

An experiment was conducted to evaluate the impacts of exogenous application of synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) on lentil (Lens culinaris M.) growth, yield, and nutritional parameters in the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh. Six treatments of 2,4-D (T0 = 0; T1 = 0.3; T2 = 0.5; T3 = 0.8; T4 = 1; and T5 = 1.2 ppm 2,4-D) with three replications were used in this study. Data on growth, yield, and nutritional parameters were collected and analyzed. The response of exogenous application of 2,4-D was significant in improving the performance of all the growth and yield contributing parameters as compared to untreated control plants. Exogenous application of 1 ppm 2,4-D was found to be effective for increasing plant height, branch number, root length, root-shoot fresh, and dry weight, nodule number, flower number, pod number, pod length, number of grains plant-1, 100-grain weight, and yield. Exogenous 2,4-D treatment significantly reduced the maturity time of lentil grain. The lowest date of maturity was found at 0.8, 1.0, and 1.2 ppm 2,4-D treatments. In this study, results showed a significant increase of different macro- (N, P, K) and micro- (Fe, Mn, Zn) nutrient content, uptake, and protein content in lentil grains with 1 ppm 2,4-D. It has been also revealed that the minimum requirement of 2,4-D to produce 80% lentil yield was 0.83 ppm. As a result, it was determined that the exogenous application of 2,4-D treatments is promising for enhancing the growth and productivity of lentil. [ABSTRACT FROM AUTHOR]

The popular Joha rice cultivar Kon Joha, cultivated throughout the state, was used to induce mutations. Kon Joha is short-grain aromatic rice with excellent cooking quality. The cultivar is tall and late maturing with an average yield of less than 2 tons ha−1 and is susceptible to lodging The investigation through M1 to M3 generation aimed at improving the morpho-agronomic traits of the popular Joha rice cultivar Kon Joha. The experiments were carried out during the Sali season (winter rice) from 2017 to 2019 at Instruction-cum-Research (ICR) Farm of Assam Agricultural University, Jorhat (Assam). Dry uniform seeds of Kon Joha were subject to irradiation with 100–400 Gy of gamma rays from a 60Co source. The M1 generation used a randomized complete block design with four replications during Sali 2017. A total of 5998 M1 plant progenies were subject to screening in the M2 during Sali 2018. The M3 included 662 morpho-agronomic variants raised in the plant rows during Sali 2019, and 66 mutants were confirmed. The M1 of Kon Joha registered a reduction in germination, seedling height, pollen/spikelet fertility and plant survival at 400 Gy. All the traits showed highly significant differences among the doses in the M2 generation. The shift in trait means was in both directions as influenced by the genotype and mutagen dose. The 66 mutants exhibited significant differences for all traits in the M3 generation. Fifty mutants were shorter than the parent Kon Joha. The GCV and PCV estimates were high (>20%) for grain yield, biological yield, productive tillers, filled grains, and average panicle weight. All the traits except panicle length exhibited high heritability coupled with high genetic advance, suggesting the predominance of additive gene action and the effectiveness of simple selection. Grain yield showed a significant positive correlation with plant height, panicle length, filled grains, spikelet fertility, the average panicle weight and harvest index in the mutant population. Thus, mutation induction in Kon Joha proved useful in inducing desirable changes in plant architectural traits. The study further emphasized the short stature high yielding mutants with strong aroma for wide-scale testing in the state. [ABSTRACT FROM AUTHOR]

Lotus corniculatus L. (bird's foot trefoil, BFT) is a valuable perennial legume forage species due to its high nutritive value, persistence under grazing, and condensed tannin content that improves ruminant production and prevents bloating. However, it is less preferred by farmers compared with other perennial forage legumes such as alfalfa because of slow germination, slow establishment and low seedling vigor. This study was conducted to determine whether X-ray seed priming could improve these deficiencies. Seeds of L. corniculatus cv. 'AC Langille' were irradiated at 0, 100, and 300 Gy. Non-irradiated and irradiated seeds were sown on Murashige and Skoog/Gamborg medium under in vitro conditions and cultured for 21 days. Germination percentage, mean germination time (MGT), germination rate index, length of shoot and root, fresh and dry weight of shoot and root, dry matter ratios of shoot and root, water content of shoot and root, and seedling vigor index were measured. The results of this study demonstrated that X-ray seed priming significantly increased the germination percentage of L. corniculatus, increased the germination rate and thereby shortened the MGT, and improved seedling growth. However, X-ray pretreatment also decreased seedling shoot and root biomass. In this study, it is reported for the first time that X-ray seed pretreatment has the potential to address important seedling establishment issues in L. corniculatus. [ABSTRACT FROM AUTHOR]

Two new phenylpropanoids (1 and 2) and one new isoflavone glycoside (3), along with nine known compounds (4 − 12), were isolated from the pod of Ceratonia siliqua L. Their chemical structures were elucidated based on extensive spectroscopic analyses (1 D and 2 D NMR, UV, IR, and HRESIMS) and compared with the literature data. In addition, all isolated compounds were evaluated in vitro for inhibitory activity against acetylcholinesterase (AChE). Compounds 4, 5, and 12 showed inhibitory activity against acetylcholinesterase (AChE) with IC50 values ranging from 15.0 to 50.2 μM. [ABSTRACT FROM AUTHOR]

To verify the hypothesis about the preservation of signs of radiation-induced genomic instability at the flowering stage of the chamomile plant after pre-sowing seed irradiation, the interaction of dose-dependent changes in the level of DNA damage and stimulation of antioxidant production. The study was carried out on two genotypes of chamomile, Perlyna Lisostepu variety and its mutant, using pre-sowing seed radiation exposure at dose levels 5–15 Gy. Studies of the rearrangement of the primary DNA structure of under different doses were studied on plant tissues at the flowering stage using – ISSR and RAPD DNA markers. Dose-dependent changes relative to the control of the amplicons' spectra were analyzed using the Jacquard similarity index. Antioxidants such as flavonoids and phenols were isolated from pharmaceutical raw materials (inflorescences) using traditional methods. Preservation of multiple DNA damages at the stage of plant flowering under pre-sowing seed irradiation at low doses was confirmed. It was found that the largest rearrangements of the primary DNA structure of both genotypes, manifested in reduced similarity with the control spectra of amplicons, were observed under irradiation dose levels 5–10 Gy. There was a tendency to approach this indicator to the control under 15 Gy dose, which means increasing efficiency of the reparative processes. The relationship between the polymorphism of the primary structure of DNA by ISSR-RAPD-markers in different genotypes and the nature of its rearrangement under radiation exposure was shown. Dose dependences of changes in the specific content of antioxidants were non-monotonic with a maximum at 5–10 Gy. Comparison of dose dependences of changes in the coefficient of similarity of the spectrum of amplicons between irradiated and control variants with nonmonotonic dose curves in the specific content of antioxidants allowed to suggest that there was the antioxidant protection stimulation under the doses corresponding to low efficiency of repair processes. The decrease in the specific content of antioxidants followed the restoration of the genetic material normal state. The interpretation of the identified phenomenon has been based on both known connection between the effects of genomic instability and the increasing yield of the reactive oxygen species and general principles of antioxidant protection. [ABSTRACT FROM AUTHOR]

The current debates in the area of researchers' positionalities criticize the notion of the 'insider/outsider' dichotomy and favour the idea of a fluid inbetweener position. However, these narratives foreground researchers' perspectives and often ignore participants' agency in constructing a researcher's positionality in the field. In this paper, as an early career researcher, I analyze my journey with my own positionalities in ethnographic research in a rural community in Bangladesh. Adopting a Critical Realist ontological standpoint, I argue that positionalities are co-constructed by researcher and participant and are products of complex interactions between their agencies and the social structure. I illustrate how reflexivity, taking both my and the participants' views into account, facilitated my movement towards a position where the participants expose their habitual behaviour (not hesitating to offer their day-to-day food – mash potato) rather than providing superficial information (as they do to a guest, for whom they will at least fry an egg – a special arrangement – for dinner). [ABSTRACT FROM AUTHOR]

The article discusses the challenges faced by alternative food networks as they grow in size and attract potential buyers. It highlights the success story of Timeless Seeds, a group of renegade farmers in Montana who chose to grow legumes and employ agroecological farming practices. Timeless Seeds developed an underground marketing network that bypassed conventional food chains and built a multi-million dollar business. Recently, Timeless Seeds was acquired by Mad Agriculture, a nonprofit aiming to improve the ecosystem for farmers transitioning to regenerative organic farming. The article emphasizes the importance of safeguarding the interests of farmers and maintaining the integrity and values of alternative food businesses. [Extracted from the article]

CPG's wastewater from phosphate ore processing was characterized and its phytotoxicity before and after physicochemical treatment inside washing units was investigated for the first time. Mineralogical, spectral, physicochemical and microbiological characterizations showed that these wastewaters are relatively alkaline due to the high calcium and phosphorus contents. In fact, numerous minerals mainly fluorapatite, clinoptilolite and gypse were identified. In addition to its high salinity (EC = 9890 µS cm−1), the BOD5/COD ratio is equal to 0.949, which allows for the estimation that this effluent is potentially biodegradable. The microbiological characterization revealed the presence of revivable aerobic bacteria as; total coliforms (TC), fecal coliforms (FC), Pseudomonas, anaerobic sulfite bacteria (ASR), and fungi (F), whereas a complete absence of Salmonella, Vibrio cholera (CV), and Helminth egg (HE) was noted. In the second part, the influence of varying concentrations of phosphate wastewater was studied on radish, lentil, wheat and fenugreek seeds. Phosphate effluents were provided at 10%, 25%, 50%, 75% and 100%. Results were compared with a control group of the same seeds, irrigated with tap water. Based on several germination tests, plant irrigated with treated or untreated effluent exhibited a prominent reduction in growth traits at all four harvests. Except plant treated with treated effluent at 25% which generally induced no significant phytotoxicity to all species and which can play a fertilizer role. The adverse effects occurred with effluent-dose dependent manner. The high toxicity of untreated effluent compared to the treated effluent was inferred from the results. Nevertheless, the treated ffluent is still toxic, except at very low concentrations acting as a fertilizer. [ABSTRACT FROM AUTHOR]

Many aspects have come to light for sustainable agriculture system to maintain soil status. Integrated nutrient management (INM) is a concept which combines the use of organic and inorganic sources of nutrients to fulfil plant nutrient requirement and for growing crops in a sustainable way which ultimately maintains soil health as well as to supply nutrient in appropriate amount which consider social, ecological, and economic impacts. This review particularly shows the positive impact in agricultural production of lentil as well as its importance and need in agriculture production. INM practices have some beneficial impacts on growth and yield parameters of lentil. The nodule formations of legumes crop have good impact on nutrient management. Lastly, this review paper also defines about the relation of biofertilizers on crop production and its effect on different parameters of lentil production which can be combined with other nutrient management strategies to be considered as an improved technology. [ABSTRACT FROM AUTHOR]

Lentil is often exposed to drought stress during the reproductive phase in arid and semi-arid regions. Shifting the sowing date of lentil plants from spring to fall prevents the reproductive phase from exposure to drought stress. Nevertheless, winter frosts limit lentil productivity in the fall sowing. Twenty lentil genotypes were studied in two locations (Mashhad and Jolgeh Rokh, Iran) during 2018–2019. The results showed a 100% survival in all genotypes in Mashhad due to the lack of extreme cold. While the temperature of −12.4°C in Jolgeh-Rokh in February resulted in variable plant survival rates; the highest survival rates were observed in MLC103 (45.2%). In Mashhad, plant height and the number of lateral branches of genotypes were ~15 and 52%, respectively, greater than those in Jolgeh-Rokh. The greatest biological and grain yield was observed in MLC33 and MLC70, respectively, in Mashhad and Jolgeh-Rokh. However, none of the genotypes could produce more than 1000 kg ha−1 grain yield, which might be due to the Ascochyta blight disease in Mashhad and extreme cold in Jolgeh-Rokh. Cluster grouping showed that the MLC409 and MLC70 were selected in cold regions, while the MLC33, MLC47, MLC409, and MLC454 were recommended for temperate climates. [ABSTRACT FROM AUTHOR]

Germination of seeds is a complex process linked to various molecular and biochemical changes that increase the nutritional value. This research has studied the impact of germination on the enzymatic and non-enzymatic antioxidant activities of five domestic staple legumes from Pakistan: chickpea, mung bean, soybean, lentil, and peanut. The maximum moisture content was recorded for chickpea, i.e., 11.7 ± 0.05 g 100 g−1 followed by lentil, soybean, peanut, and mung bean as 9.65 ± 0.02, 8.07 ± 0.01, 7.6 ± 0.01, and 7.31 ± 0.01 g 100 g−1, respectively. The overall augmentation in catalase activity during germination (days 1 to 4) was maximum for lentils, i.e., 76.22%, and ascorbate peroxidase activity for peanuts, i.e., 62.09%. The soybean showed a clear difference from the 1st to 4th days of germination for peroxide activity among all selected legumes with a 37.29% increase. A maximum change in superoxide dismutase was noticed in mung bean. The mung bean also had the highest variation in total flavonoid contents, while total phenolic contents were higher in the chickpea. The radical scavenging activity varied higher in peanuts, while the ascorbic acid content was maximum for soybeans. Among all selected legumes, non-germinated chickpeas showed a lower DPPH value, i.e., 1.15 ± 0.03%, whereas the highest DPPH value was observed in lentils (69.84 ± 0.05%). Thus, the incorporation of ready-to-eat (RTE) germinated legumes with high antioxidant activity would be a contemporary addition to the everyday meal. [ABSTRACT FROM AUTHOR]

Aflatoxins are types of mycotoxins that can cause acute and chronic toxicity in human. In general, there are four types of aflatoxinsB1, B2, G1 and G2, which among them aflatoxin B1 is the most potent carcinogen in animal species and causes liver cancer. The goal of this study was to evaluate of aflatoxin concentration in legumes samples marketed in Tehranbya high-performance liquid chromatography (HPLC). Fifty samples of legumes including peas, red beans, lentils, mung bean and cotyledons (n = 10 of pea, n = 10 of red bean, n = 10 of lentils, n = 10 of mung bean and n = 10 of cotyledons) were obtained from different regions (bulk legumes) of Tehran city in Iran and different packaging brands that had the highest supply in Tehran stores and were transported to the laboratory under appropriate conditions to measure aflatoxin (B1, B2, G1, G2 and total). Considering the limit of detection (LOD) of the current study, the aflatoxin level of all samples of peas, red beans, lentils, mung bean and cotyledons samples was lower than LOD. Based on the results, it can be concluded that the consumption of legumes in Tehran city by consumers can be safe, but more research is needed to avoid potential risks, and also routine and periodic monitoring of aflatoxin levels in legumes is recommended. [ABSTRACT FROM AUTHOR]

The RADARSAT Constellation Mission (RCM) can acquire imagery in Compact Polarimetric (CP) mode. With this new mode, and the increased revisit with three satellites, RCM can contribute to operational crop monitoring at national scales. The four Stokes (S0, S1, S2 and S3) and three m-chi decomposition (surface, double bounce, volume) parameters were used to identify crops (pasture/forage, barley, wheat, canola, flaxseed, peas, lentils) with a Random Forest classifier. The Stokes and m-chi parameters delivered maps of similar accuracies (95% overall accuracy) and were only slightly less accurate than a classification using optical satellite imagery (97%). To understand why Stokes parameters worked well in classifying crops, scattering responses for wheat, canola, lentils and peas were plotted on the Poincaré sphere. These responses were interpreted in the context of the degree of polarization and were related to crop phenology. These plots revealed that early and late in the season the polarized component of the scattered wave remained circular. However, in the active season when crop structure was changing, scattered waves became more elliptically polarized. Although the amount of polarized scattering was lower mid-season, the change in ellipticity was helpful in separating crop types. [ABSTRACT FROM AUTHOR]

Consumption of legumes has long been linked to their nutritional and medicinal benefits. Vigna unguiculata (L.) Walp. (Cowpea) is a legume plant in the Fabaceae family and is a rich source of nutrients also is known for its beneficial effects for diseases treatment. In terms of phytochemicals analysis and bioactivities evaluations the major research has focused on the Cowpea seeds, whereas leaves and pods are remained understudied. Herein we have highlighted leaves methanolic extract phytochemicals identification, antimicrobial, and antioxidant activity assessment. Cowpea leaves methanolic extract Liquid Chromatography-Mass Spectrometry (LC-MS) analysis first time revealed the presence of α-hederin, which is a putative novel SARS-COV-2 inhibitor and Zearlenone mycotoxin. Leaves methanolic extract exhibited strong activity against Streptococcus pyogens and Candida albicans. The Cowpea leaves extract is a potent DPPH inhibitor with an IC50 of 62.04 ± 0.08 μg/mL. The bioactive compounds identification in this work supports the plant's nutritional and medicinal uses. [ABSTRACT FROM AUTHOR]

In this paper, the phytochemical analysis of the seeds of a new Iranian Plantago ovata Forssk. population exemplar is reported. This phytochemical analysis was carried out by means of hydroalcoholic maceration, column chromatography, NMR and MS analyses and led to the isolation of sixteen compounds belonging to five different classes of natural compounds. After comparison with previous analyses, a clear difference about their whole phytochemical patterns could be observed in some terms. A possible explanation of this was generally given, too. [ABSTRACT FROM AUTHOR]

Proso millet is a potential crop for food, nutritional security, and sustainable agriculture, particularly in the context of climate change. It is one of the traditional millet crops in cultivation in Tamil Nadu and India. Self-pollinated nature of this crop makes evolutionary process a terminal one and creating variability to broaden the genetic base is important. The objective of the study was to optimize mutagenic dose of gamma mutagen, document types of mutations and identify mutants for high grain yield (GY) and fodder yield (FY), hence to determine the feasibility of gamma mutagenesis in proso millet crop improvement. A mutation breeding program with 10 doses of gamma irradiation, i.e. 100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 Gy were imposed on seeds of variety ATL 1, a popular variety in India. Roll paper method, tray method and field evaluation were carried out to determine the LD50 and GR50 doses. Based on viable mutation frequency in M2 generation, mutagenic effectiveness and efficiency was estimated. Targeted selection for yield and yield contributing traits was carried out in M2, M3 and M4 generation to identify high yielding mutants. The LD50 and GR50 doses of gamma were estimated to be 418 Gy and 542 Gy, respectively. Based on results from probit analysis, mutagenic effectiveness, mutagenic efficiency and mutation spectrum, the optimum treatment dose of gamma was concluded to be 400 Gy. High frequency, i.e. 10.96% of phenotypic mutants was identified in the M2 generation. The broad range of mutants identified in M2 generation had mutations for plant height, plant habit, panicle shape, compactness, and length, days to 50% flowering (DFF), lodging resistance, tillering, leaf phenotype, apiculus color, culm branching, stem and leaf hairiness, sheath pigmentation, seed color and shape and seed coat attachment. Eight high yielding mutant families were isolated in M4 generation. The mean single plant GY and FY of these mutants ranged between 25 to 51 g and 40 to 68 g respectively while in control it was 15 g and 30 g, respectively. The wide spectrum and high frequency of mutations both for qualitative and quantitative traits suggest mutation induction as a promising method for creating novel variations in proso millet. The high yielding mutants identified can be utilized for varietal development both for grain and fodder purpose after further stability and quality evaluations in the advanced generations. [ABSTRACT FROM AUTHOR]

Soil-test crop response (STCR) approach is aiming to precise adjustment of fertilizers under varying soil-test values for targeted-yield, presently gaining recognitions for sustainable nutrient management. The study aimed to envisage the precision scale of STCR targeted-yield models in maize-lentil rotation in alkaline Fluvisol under sole-fertilizer and integrated nutrient management (with farmyard manure). Fertility-gradient, test-crop diagnostic, and validation trials were conducted following STCR technical program. Soil fertility gradient and variable fertilizer doses had caused a large variation in grain yield of maize (0.99–4.94 t ha−1, CV = 30%) and lentil (1.11–2.02 t ha−1, CV = 13%). Lentil had increased potential to utilize soil nutrient pools; in contrast, maize was highly responsive to fertlizer nutrients. Multivariate-regression analysis revealed that fertilizers N, P, and S had a strong and direct influence on maize yield; where fertilizer-P was the primary yield-determining variable for lentil. Both sole-fertilizer and integrated STCR-based treatments attained the targeted-yields in maize. However, STCR treatments could not attain the targeted-yields in lentil (17–29% deviation), but had yield advantage over the blanket recommended fertilizer dose. Hence, in tropical-alkaline soils, integrated STCR targeted-yield approach could be the sustainable nutrient management option(s) for improving the productivity of the maize-lentil system. [ABSTRACT FROM AUTHOR]

Grain legumes are indispensable sources of plant-based protein, predominantly grown for their edible seeds. They are characterized with their exclusive property of symbiotic nitrogen fixation in root nodules in association with Rhizobium bacteria. Balanced supply of micronutrients has been manifested to be as important as macronutrients for extensive growth and development of grain legumes coupled with improved Rhizobial activities. Among those micronutrients, molybdenum (Mo) is of prime concern. It acts as a cofactor for nitrogenase and nitrate reductase enzymes, which are directly involved in biological fixation and subsequent assimilation of nitrogen in legumes. Thus, Mo plays a crucial role in nitrogen metabolism and protein synthesis. It also facilitates diverse physiological and biochemical processes including photosynthesis as well as carbohydrate and sulfur metabolisms. Optimization of Mo nutrition is invariably linked with accelerated phyto-availability of other essential nutrients to crops. Nevertheless, positive effects of Mo fertilizers tend to be more pronounced under acid soils where plant available forms of Mo are negligible. Research efforts have proven the dynamic significance of Mo in magnification of physiological growth, yield potential and nutritional qualities of miscellaneous grain legumes including chickpea, lentil, pea, grass pea, red gram, black gram, green gram, common bean, soybean, groundnut, cowpea, cluster bean, horse gram and hairy vetch. Studies have also shown that, application of Mo is cost-effective owing to its minimum dosage applied. Therefore, the recommendation for Mo nutrition is a definite step forward for profitable cultivation of grain legume crops while maintaining the fertility status of soil. [ABSTRACT FROM AUTHOR]

The aim of present study is bread enrichment by adding germinated lentil flour to Sangak bread. In this study, the dried germinated lentil flour was replaced with 7% moisture at four levels of 0, 5, 10, and 20% by weight in wheat flour. High germinated lentil flour content caused the hardness of the bread texture was increased significantly. Farinograph test revealed that water absorption, development time of the dough, and the softness degree of the dough increased by adding the lentil flour but the stability of the dough was constant. Sensory evaluation showed that control treatment and the 5% treatment have the most score of qualitative number. Increasing the germinated lentils flour in bread treatments led to increasing the amount of protein, iron, fiber, and ash content in treatments. In the 20% treatment, vitamins B3 and B6 were observed to be 26.6% and 70% of the initial amount, respectively. [ABSTRACT FROM AUTHOR]

Light is considered as an environmental signal that controls plants growth and development. Sufficient duration, quality and wavelength are mainly responsible for switching on/off many physiological processes. The aim of this study was to examine the role of gamma aminobutyric acid (GABA) shunt pathway specific response in wheat (Triticum aestivum) and lentil (Lens culinaris) seedlings to blue LED laser (intensity= 1.48 W/cm2) and high intensity invisible ultraviolet light (intensity= 3.99 W/cm2) treatments separately, with respect to: seed germination, seedling growth, oxidative damage in term of reactive oxygen substances accumulation, GABA accumulation level, total proteins and total carbohydrates level and glutamate decarboxylase gene (GAD) expression. Data showed a remarkable changes in proteins content, carbohydrates content, chlorophyll level, dry and fresh weight, GABA accumulation, MDA level, and GAD m-RNA level under blue LED laser and high intensity invisible ultraviolet light treatments; separately in the both wheat and lentil cultivars. There were no significant differences of root emergence in all cultivars under all treatments. The concentrations of MDA increased in all cultivars under blue LED laser and high intensity invisible ultraviolet light treatments with increasing exposure durations. GABA levels increased and correlated significantly in all cultivars as the exposure duration of blue LED laser and high light intensity increased. Significant decrease in chlorophyll a and b contents in Um Qayes, Jordan1 and Jordan2 cultivars under all laser and high intensity invisible ultraviolet light treatments. A significant difference was found in proteins content in all cultivars in response to laser and high intensity invisible ultraviolet light treatments. Carbohydrates content in all cultivars used in this study were significantly decreased under laser treatments. The transcription level of GAD increased significantly under blue LED laser and high intensity invisible ultraviolet light in all cultivars used in this study. GAD expression was high in Hurani75 cultivar in agreement with the low level of GABA accumulation under high intensity invisible ultraviolet light at 60 min which strongly suggested that GABA was catabolized to enter another pathways (C:N balance) that was confirmed by increase in higher level of carbohydrates and proteins beside reduction in MDA content at the same exposure duration. On other hand, GAD expression in Jordan 2 was lower in response to laser treatment under the same exposure duration. The high increase of GAD gene expression explains the high accumulation of GABA level under both treatments. Results indicated that GABA shunt is a key metabolic pathway that allows wheat and lentil to adapt blue LED laser and high intensity invisible ultraviolet light stressful treatments. [ABSTRACT FROM AUTHOR]

In the current study, use of cost effective nitrogen source in the production of protease was aimed by using Bacillus subtilis ZBP4 which is an indigenous strain capable of producing various enzymes and industrially important metabolites. Lentil flour (LF) was used for this purpose, and enzyme production from LF was compared with some other nitrogen sources including casein, yeast extract, peptone from meat, skim milk powder, whey powder, and soy bean flour. Enzyme production in 48 h with LF (863 ± 21.4 U/mL) was lower than only yeast extract (2344 ± 24 U/mL) and soy bean flour (1424 ± 67.6 U/mL). On the other hand, it was considerably higher than all of the other nitrogen sources. Effect of LF concentration (5–20 g/L) was determined by using only LF as nitrogen source and the highest enzyme production was obtained at 20 g/L concentration (1000 ± 29.2 U/mL). Effects of pH (5–9) and temperature (30–45 °C) were also determined and the optima were found as pH 8 and 30 °C. As a consequence, LF can be a candidate as a nitrogen source for protease production by Bacillussubtilis ZBP4. [ABSTRACT FROM AUTHOR]

Colletotrichum lentis is an important pathogen of lentils in Canada causing the disease anthracnose. Fungicide applications are an important tool for the control of this disease and, historically, QoI fungicidegdels have been used intensively. Reduced field efficacy of QoI fungicides on C. lentis has been reported recently and could be linked to the presence of QoI resistant isolates. In this study, the cytochrome b gene of C. lentis was sequenced, and the mutation G143A was identified as the causal resistance mechanism. Furthermore, a sensitive and reliable molecular biological assay was developed for the quantitative detection of the G143A mutation by pyrosequencing in field samples. [ABSTRACT FROM AUTHOR]

To study the distribution of 210Po concentrations in various parts of spice plants cultivated by conventional farming in Nilgiris, South India and estimate effective radiation dose received. Parts of plant such as leaf, fruit, seed, flower bud and rhizome of selected spice plants cultivated at various locations in the study area have been collected for estimation of 210Po concentration. Organic materials in known mass of processed samples have been removed by chemical method. 210Po concentration in the digested sample has been electrostatically deposited on pre-polished silver disks at constant temperature. ZnS(Ag) detector-based alpha counting system with minimal detectable value 3.2 mBq has been used for estimation of 210Po activity concentration. Leaves of Curcuma longa have registered a high concentration of 45.6 ± 4.5 Bq kg−1 of 210Po and rhizome of Allium sativum has registered a low value of 4.7 ± 0.8 Bq kg−1. Leaves of spice plants have registered higher concentration of 210Po than their fruits, seeds, flower buds or rhizomes. Soil to plant transfer factor of 210Po has been found to vary from 0.13 for rhizome of A. sativum to 2.23 corresponding to leaf of C. longa. Concentration of 210Po estimated in leaves and mean leaf areas of spice plants has been found to have a correlation coefficient of 0.83, which indicates that absorption of 210Po deposited on surface area of leaves is the main source of this isotope in plant parts. Consumption of various parts of spice plant does not pose significant radiological risk as effective dose received by the general public as 210Po has been found to vary from 6.34 to 46.05 µSv y−1, less than the value recommended by ICRP. [ABSTRACT FROM AUTHOR]

Mungbean yellow mosaic India virus (MYMIV) is a serious constraint in the mungbean which is a potential source of easily digestible high-quality proteins, fibers, minerals, and vitamins in Asian countries. Developing resistant cultivars is the most cost-effective, eco-friendly, and sustainable approach to protect mungbean from MYMIV damage. Mutation breeding provides a quick and cost-effective way of developing resistance as lack of genetic variability is the biggest bottleneck for other traditional breeding tools. Outstanding but MYMIV-sensitive varieties of mungbean, viz., MH 2-15 and MH 318 were mutagenized through various individual and combined doses of gamma-rays and Ethyl methanesulfonate (EMS) and evaluated in M2 and M3 generations for the appearance of resistance reactions. This was subsequently validated through marker-assisted genotyping using previously reported Yellow Mosaic Disease (YMD) linked markers. The phenotyping in M3 generation yielded 64 MYMIV resistant mutants whereas, marker-assisted genotyping identified the 22 mutants with true resistance. Markers YR4, CYR1, and CEDG180 were found associated with MYMIV resistance whereas, DMB-SSR158 did not show any amplification. Among identified resistant mutants, ten lines exhibited at par and two revealed a little higher seed yield over controls. The mutagenesis created significant variability in MYMIV resistance as well as seed yield per plant. YR4, CYR1, and CEDG180 are found to be linked with the MYMIV loci in the mungbean and could be utilized for MYMIV resistance breeding. Mutant M-37 from MH 2-15 and M-104 from MH 318 exhibited more seed yield along with MYMIV resistance which upon further validation can be released as a variety. The induced mutagenesis integrated with powerful emerging molecular and next-generation sequencing (NGS) tools would be highly helpful in breeding mungbean for durable resistance against threatening MYMIV. [ABSTRACT FROM AUTHOR]

Thermal properties of lentil (Lens culinaris) and chickpea (Cicer arietinum L.) were determined experimentally and with predictive mechanistic models as functions of temperature and moisture content (four levels). Thermal conductivity (k), specific heat (Cp), and density (ρ) of the samples were evaluated using a line heat source probe, differential scanning calorimeter (DSC), and pycnometer, respectively. Except for Cp which was measured at a temperature range of 30 to 90°C, other properties were measured at room temperature. Specific heat of the samples increased linearly with moisture content (MC) and temperature, ranging from 0.824 to 2.433 kJ/kg K and 0.444 to 2.067 kJ/kg K for lentil and chickpea, respectively. Thermal conductivity increased with MC in all samples with its values ranging from 0.161 to 0.191 W/m K for lentil and 0.160 to 0.227 W/m K for chickpea. However, thermal conductivity values of flours were higher at lower MC levels when compared to seeds at higher MC levels. Thermal diffusivity, (0.159 × 10−6 to 0.221 × 10−6 m2/s and 0.163 × 10−6 to 1.175 × 10−6 m2/s for lentil and chickpea flours, respectively) was calculated from known values of k, Cp, and density (ρ), with its values decreasing as the MC levels increased. Thermal properties data from our experiments did not fit into the components-based mechanistic models. Models generated in this study have good significance (p<.05 and R2 ≈ 1), meaning they can be used for prediction of these properties, as well as modeling and simulation of thermal behavior of pulses during conventional or radio frequency (RF) heating. [ABSTRACT FROM AUTHOR]

The seeds and oil yield and profile/levels of fatty acids, tocopherols and phytosterols in seed oils of twelve Japanese quince (Chaenomeles japonica) genotypes were studied. The seeds and oil yield ranged from 3.8 to 5.7% w/w fresh fruit, and 10.9 to 14.6% w/w dry weight seeds, respectively. The range of three predominated fatty acids C16:0, C18:1 and C18:2 in the seed oil of twelve Japanese quince genotypes were 8.1–9.8, 37.5–48.1, and 40.1–50.3%, respectively. α-Tocopherol and β-sitosterol were the main minor lipophilic compounds detected in all investigated genotypes. The percentage of predomination of α-tocopherol and β-sitosterol in each investigated genotype was very similar and amounted to 97–99% of total tocopherols and 76–80% of total phytosterols, respectively. The range of total content tocopherols and phytosterols in 12 genotypes of Japanese quince were 91.9–129.3 and 7830–14166 mg/100 g oil, respectively. [ABSTRACT FROM AUTHOR]

Momordica charantia is a medicinal plant which is widely used in different traditional medicinal systems to treat several diseases. We have identified the differential distribution of phytomedicinally important metabolites in the pericarp, skin and seeds of M. charantia fruit via NMR spectroscopy. Multivariate statistical analysis showed a clustering of the metabolic profiles of seeds and pericarp, and their clear separation from the metabolic profile of the skin. The total phenolic and flavonoid content of the fruit extracts were estimated via bioassays, the radical scavenging activity was estimated via in vitro DPPH and ABTS assays and an inhibitory activity test of α-glucosidase was also performed. The pericarp and seeds contained significant amounts of phenolic compounds and flavonoids, indicating that they are a good source for antioxidants. The skin contained a significantly higher amount of phytosterols such as Charantin and momordicine, which are known to correlate with antidiabetic activity. [ABSTRACT FROM AUTHOR]

Management of broomrape (Orobanche crenata Fosk.) that causes important damages on lentil production becomes a veritable concern in the Mediterranean region. Eighty lentil accessions were evaluated for resistance to O. crenata under field and controlled conditions. Both genotypes ILL6415 and ILL7723 expressed the highest resistance level under field and pot experiment with low Orobanche infestation and relatively high seed yield (50.1 g m−2). Such resistance was associated with physiological and biochemical changes in metabolites profiling. In total, 109 and 115 metabolites were identified in the lipophilic phase of both ILL6415 and ILL7723, respectively, against only 92 metabolites recorded for susceptible check Zaaria. Significant differences were observed in metabolite concentrations (fatty acids, sterols alkanes) between roots and shoots of susceptible and resistant infested plants. Accumulation of α-linolenic acid and arachidic acid was more pronounced in the resistant genotypes ILL6415, ILL7723 which could be associated with resistance pathways involved in the resistance to O. crenata. [ABSTRACT FROM AUTHOR]

Bioremediation using microorganisms have become innovative efficient tools for detoxifying a wide variety of urban and industrial wastewaters. In this study, we investigated for the first time the microbial detoxification of phosphate processing wastewaters (PPWW) by using the indigenous bacterium Serratia rubidaea NCTC12971, having numerous plant growth promoting traits. In fact, the isolated strain exhibited an attractive capacity of phosphate solubilization, siderophore production as well as indole acetic acid (IAA) secretion. Additionally, the strain has significant antifungal effect against Alternaria alternata. The monitoring of the bacterial growth at increasing concentrations of heavy metals showed minimum inhibitory concentrations (MICs) of 8, 6, 3 and 3 mg L−1, respectively for Pb, Cd, Cu and Zn. The 10-days treatment of PPWW by Serratia rubidaea significantly decreases its salinity in terms of electrical conductivity (EC), its chemical oxygen demand (COD), its biochemical oxygen demand (BOD5) and its total organic carbon (TOC). Regarding heavy metals removal, the detoxification rates were 94.4, 91.5, 85, and 66.5%, for Pb, Cd, Cu, and Zn, respectively. Moreover, the biological treatment of the PPWW declines its phytotoxicity toward lentils (Lens culinaris) and chickpea (Cicer arietinum) seeds germination. Hence, with the PPWW, the germination indices (GI) of chickpea (Cicer arietinum) and lentils (Lens culinaris) were 16.6 and 10%, respectively. While, with the biological treated (BTPPWW), the GI were significantly increased to approximately 76.6% for both seeds mentioned. Such results strengthened the potential use of Serratia rubidaea NCTC12971 for the establishment of biotechnological process aiming to minimize the harmful effects of PPWW as well as its use as plant growth promoting bacteria (PGPB). This study is the first report investigating the potential use of indigenous bacteria in the bio-treatment of wastewaters discharged from the Tunisian phosphate laundries units. Serratia rubidaea NCTC12971 was selected as a potent applicant for heavy metals detoxification. Serratia rubidaea NCTC12971 was characterized by its plant growth promoting traits and antifungal activity against Alternaria alternata. [ABSTRACT FROM AUTHOR]

Under cool and wet conditions, high levels of botrytis grey mould (BGM) can occur on lentil in western Canada. Identification of local Botrytis isolates from lentil based on conidial morphology (N = 74) and sequence analysis (N = 5) indicated that B. cinerea was the predominant cause of BGM in this region. Eight field experiments at two sites (Saskatoon, SK and Outlook, SK) over two seasons were conducted to evaluate the optimal timing of the fungicide boscalid for the control of BGM in the lentil cultivar 'CDC Grandora'. Weather conditions limited disease development in one year, but moderate levels were observed in four experiments in the other year. In all experiments, a single application at mid-flower in early-seeded experiments and at early flower in late-seeded experiments was as efficacious as a double or triple application of boscalid. There were no differences among double application treatments, and at Outlook they were all as efficacious in lowering BGM as the triple application. Despite some differences in BGM severity among fungicide applications, none of the treatments had an effect on yield or seed infection, and there was no correlation between petal colonization by B. cinerea and BGM disease severity under the study conditions. [ABSTRACT FROM AUTHOR]

Salinity is a major abiotic stress limiting plants growth and development. As silicon (Si) has been stated to alleviate salt stress in some plant species, effects of Si on Lens culinaris Medik under salinity were investigated in this study. The effects of four levels of Si in nutrient solution (0, 0.1, 0.25, and 0.5 mM) on the plant growth and physiological parameters under salinity (0, 40, and 80 mM of NaCl) were considered in a factorial experiment. Salinity decreased dry weight, stem length, leaf area, chlorophyll and anthocyanin content, potassium (K) and protein concentration, K to sodium (Na) ratio and root Si content, while increased carotenoids content. Si, especially at 0.1 mM concentration in nutrient solution, increased root and shoot length, plant dry weight and leaf area. However, higher concentrations of Si often had a negative impact on most of the characteristics. Also, some levels of Si treatment significantly increased leaf chlorophyll, carotenoids and anthocyanin, K, Si and protein content but decreased the amount of plant Na content under salinity. In overall, it seems that, the use of low levels of Si is capable to reduce harmful effects of salinity on L. culinaris and improve plant resistance to salt stress. [ABSTRACT FROM AUTHOR]

Intercropping medicinal plants with grain legumes provides an excellent opportunity for small-scale farmers to generate more income and move towards sustainable cropping systems. The effect of arbuscular mycorrhizal fungi (AMF) inoculation on yield, quality and nutrient uptake of isabgol and lentil, when grown as monocrops or in various intercropping ratios, were evaluated. Inoculation with AMF resulted in positive effects on AMF root colonisation, yield components and seed yields (significant for lentils only) of both crops, especially in the intercropping systems, as well as in the nutrient uptake in isabgol. The highest seed yields of isabgol (743 kg ha−1) and lentil (684 kg ha−1) were obtained when these species were grown as monocrops and inoculated with AMF. The highest mucilage content (20.9%) and swelling factor (16.9 ml) of the isabgol seeds were found when the two secies were grown in the intercropping ratio of 2I:2L (2 rows isabgol: 2 rows lentil) and treated with AMF. Regarding the composition of the isabgol seed mucilage, the highest concentrations of galacturonic acid (9.0%), galactose (11.8%), glucuronic acid (18.3%), and xylose (39.0%) were recorded in intercropping with AMF treatment. AMF inoculation also increased soil basic microbial respiration, soil substrate-induced respiration and soil microbial biomass carbon. Overall, the highest land equivalent ratio (LER = 1.67) was recorded in the 4I:2L intercropping pattern with AMF inoculation. The results indicated that intercropping of isabgol and lentil together with AMF inoculation was an effective system to increase the use efficiency of resources for smallholder farmers in semi-arid regions. [ABSTRACT FROM AUTHOR]

Coagulation performance of different seeds' extracts, namely, (Bean, Lima bean, Lentil, and Fenugreek) were evaluated using synthetic turbid water. The coagulation active agents were extracted from the crushed seeds using different types of solvents like sodium chloride and sodium hydroxide solutions with different molarities. Batch coagulation experiments were conducted to evaluate the activity of the extracted bio-coagulants for water decontamination. The coagulation effectiveness of the seeds' extracts has been compared with that of alum. The highest % turbidity removal using Fenugreek was 98% while that of alum was 85%. Thus, using Fenugreek as plant-based natural coagulants is a promising alternative toward sustainable water clarification. [ABSTRACT FROM AUTHOR]

Pulses are a major source for plant-based proteins, with over 173 countries producing and exporting over 50 million tons annually. Pulses provide many of the essential nutrients and vitamins for a balanced and healthy diet, hence are health beneficial. Pulses have been known to lower glycemic index (GI), as they elicit lower post prandial glycemic responses, and can prevent insulin resistance, Type 2 diabetes and associated complications. This study reviews the GI values (determined by in vivo methodology) reported in 48 articles during the year 1992-2018 for various pulse type preparations consumed by humans. The GI ranges (glucose and bread as a reference respectively) for each pulse type were: broad bean (40 ± 5 to 94 ± 4, 75 to 93), chickpea (5 ± 1 to 45 ± 1, 14 ± 3 to 96 ± 21), common bean (9 ± 1 to 75 ± 8, 18 ± 2 to 99 ± 11), cowpea (6 ± 1 to 56 ± 0.2, 38 ± 19 to 66 ± 7), lentil (10 ± 3 to 66 ± 6, 37 to 87 ± 6), mung bean (11 ± 2 to 90 ± 9, 28 ± 1 to 44 ± 6), peas (9 ± 2 to 57 ± 2, 45 ± 8 to 93 ± 9), pigeon peas (7 ± 1 to 54 ± 1, 31 ± 4), and mixed pulses (35 ± 5 to 66 ± 23, 69 ± 42 to 98 ± 29). It was found that the method of preparation, processing and heat applications tended to affect the GI of pulses. In addition, removal of the hull, blending, grinding, milling and pureeing, reduced particle size, contributed to an increased surface area and exposure of starch granules to the amylolytic enzymes. This was subsequently associated with rapid digestion and absorption of pulse carbohydrates, resulting in a higher GI. High or increased heat applications to pulses were associated with extensive starch gelatinization, also leading to a higher GI. The type of reference food used (glucose or white bread) and the other nutrients present in the meal also affected the GI. [ABSTRACT FROM AUTHOR]

To increase the size of the flowers for easy plucking, flower yield, pyrethrins content (%), and elite mutant selection in pyrethrum. To increase pyrethrum production and acclimatize in north Indian plain condition, a genetic improvement program was undertaken to widen the range of variations for size and yield of flowers and pyrethrins content (%) in pyrethrum crop. Pyrethrum seeds of the variety Avadh were irradiated with gamma rays at 20 to 300 Gy doses in Gamma chamber 5000 (cobalt-60 research irradiator). Observations gathered visually in M1 based on vigor, synchronization of flowering, and flower's size. Out of 90 M2 families, 20 mutants were raised in M3 along with the check-in preliminary evaluation trial. The four promising mutants, 1 (20 Gy-3), 7 (40 Gy-5), 10 (40 Gy-8), 14 (60 Gy19-10) was grown for four years in a bench-scale trial (randomized block design, replicated thrice) to test the yield performance and selection of high yielding elite mutant (s). It has been found that pyrethrum is sensitive to gamma rays irradiation and produced a high range of qualitative and quantitative variations. After massive screening over four years, two promising mutants for high dry flower yield and pyrethrins content, namely 7 (40 Gy-5), and 10 (40GY-8) were isolated. The mutagenesis changed traits mean in positive or negative directions. Pyrethrum plant is highly sensitive to gamma irradiation and produced a high range of variability in the qualitative and quantitative traits. The mutagenesis changed the mean of traits in both positive and negative directions. Due to mutagenic efficacy, two mutants 7 (40 Gy-5), and 10 (40GY-8) were expressed high performance for pyrethrin percent i.e., 87.23 and 59.78% improvement over the check variety 'Avadh', with synchronous flowering. These two mutants are in the pipeline for release as a variety for cultivation in the North Indian plains. [ABSTRACT FROM AUTHOR]

Root rot is a concern in the pea growing areas of Canada and elsewhere in the world, since it can cause significant yield loss. Fusarium spp. are considered major pea root rot pathogens. In this study, the pathogenicity and host range of Fusarium species collected from earlier surveys of commercial pea fields in Alberta were evaluated. Forty-five isolates belonging to six Fusarium species were selected for pathogenicity testing under greenhouse conditions. Fusarium solani f. sp. pisi and F. avenaceum were the most aggressive species, followed by F. oxysporum, F. culmorum and F. redolens. Due to their ability to cause severe disease on pea, F. solani f. sp. pisi and F. avenaceum were chosen for further testing on other common rotational crops grown in Alberta, and 10 cereal, oilseed and pulse crops were inoculated with isolates of those species. Both F. solani f. sp. pisi and F. avenaceum caused significant disease symptoms on pea, chickpea, dry bean and faba bean, but not on cereal crops, soybean, green lentil or canola when compared with their respective non-inoculated controls. Fusarium avenaceum, but not F. solani f. sp. pisi, caused significant root rot symptoms on red lentil, although disease severity was low. New information on the host range of these root rot pathogens will be valuable for choosing appropriate disease management strategies. [ABSTRACT FROM AUTHOR]

Pulses are nutrient-dense foods that have for a long time been empirically known to have beneficial effects in human health. In the last decade, several studies have gathered evidence of the metabolic benefits of pulse intake. However, it remains unclear at what amounts these effects may be attained. This study aimed to systematically review the scientific outputs of the last two decades regarding health benefits of pulse consumption and the amounts necessary for positive outcomes to be achieved. A PubMed search including keywords [("dietary pulses", "pulses", "legumes", "grain legumes", "bean", "chickpea", "pea", "lentil", "cowpea", "faba bean", "lupin") and ("inflammation", "inflammatory markers", "C-reactive protein", "blood lipids", "cholesterol", "cardiometabolic health", "cardiovascular disease", "diabetes", "glycaemia", "insulin", "HOMA-IR", "body weight", "body fat", "obesity", "overweight", "metabolome", "metabolic profile", "metabolomics", "biomarkers", "microbiome", "microbiota", "gut")] was performed. Only English written papers referring to human dietary interventions, longer than one day, focusing on whole pulses intake, were included. Most of the twenty eligible publications reported improvements in blood lipid profile, blood pressure, inflammation biomarkers, as well as, in body composition, resulting from pulse daily amounts of 150 g (minimum-maximum: 54-360 g/day; cooked). Concerns regarding methodological approaches are evident and the biochemical mechanisms underlying such effects require further investigation. [ABSTRACT FROM AUTHOR]