Search

An effective high–speed counter–current chromatography (HSCCC) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the Tibetan medicinal plantPedicularis longifloravar.tubiformis(Klotzsch) P. C. Tsoong. With a two-phase solvent system composed of chloroform–methanol–water (8:4:5, v/v), 40 mg of extract was separated to yield luteolin (12.5 mg), apigenin (9.6 mg), and chrysoeriol (4.8 mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these three components were identified by1H NMR and13C NMR. [ABSTRACT FROM PUBLISHER]

A new phenyl glycoside, 2-(3'-O-β-D-glucopyranosyl)benzoyloxygentisic acid (1), along with seven known glycosides 2-8 was isolated from Tibetan herbal medicine Lomatogonium rotatum. The structures of the compounds were elucidated by spectroscopic methods including 1D and 2D NMR techniques and MS data. [ABSTRACT FROM AUTHOR]

Both Agriophyllum squarrosum and Artemisia halodendron play essential roles in the vegetation rehabilitation process in Horqin desert. Responses of germination to temperature, water potential, and burial depth in the two species were studied experimentally. The results showed that: (1) seeds of the two species could germinate with high final percentages (> 67%) at prevailing surface soil (0-5 cm) temperatures from April to July. However, Agriophyllum squarrosum had more efficient mechanisms to ensure that seeds germinate quickly at prevailing May temperatures but slowly at the temperatures of April and July, in comparison to Artemisia halodendron; (2) Artemisia halodendron had higher final germination percentages than Agriophyllum squarrosum when water potential was low (p < .0.01); and (3) seedlings of Artemisia halodendron emerged well only at a depth less than 1 cm and did not emerge at all when the burial depth reached 4 cm, while those of Agriophyllum squarrosum emerged with a final percentage of 21.6% even at the depth of 4 cm. These different characteristics of seed germination and seedling emergence could partially explain the phenomenon that Agriophyllum squarrosum always precedes Artemisia halodendron in establishing on moving sand dunes in Horqin desert. [ABSTRACT FROM AUTHOR]

We evaluated peripheral nerve regeneration using biodegradable genipin-cross-linked gelatin nerve conduits (GGCs) with three different cross-linking degrees, 24, 36 and 51%. Biocompatibility and biodegradability of the GGC and its efficiency as a guidance channel were examined based on the repair process of a 10-mm gap in the rat sciatic nerve. From this pilot study we concluded that GGCs with a mean cross-linking degree of 36% can ensure nerve regeneration with a more mature structure, as demonstrated by better developed epineural and perineural organisation and axonal development, as well as better-recovered electrophysiology with a relatively positive sciatic functional index and a shorter latency of the muscle action potential curve. Regenerated nerves in the GGCs with mean cross-linking degrees of 24 and 51% were less favourable, due to irritation caused by degradation material and compression by the remaining tube walls, respectively. [ABSTRACT FROM AUTHOR]

First convergent synthesis (±)-glyflavanone-A (1), a novel pyranoflavanone natural product, starting from phloroacetophenone and p-anisaldehyde is described. [ABSTRACT FROM AUTHOR]