1. Identification of opportunistic parasites and helminth ova in concentrated water samples using a hollow-fibre ultrafiltration system.
- Author
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Rangel-Martínez, Cecilia, Jiménez-González, Diego Emiliano, Martínez-Ocaña, Joel, Romero-Valdovinos, Mirza, Castillo-Rojas, Gonzalo, Espinosa-García, Ana Cecilia, López-Vidal, Yolanda, Mazari-Hiriart, Marisa, and Maravilla, Pablo
- Subjects
WATER pollution ,WATER sampling ,PARASITES ,HELMINTHS ,ULTRAFILTRATION ,OVUM ,POLYMERASE chain reaction - Abstract
In the present study, we applied an ultrafiltration process to detectBlastocystisspp.,Cryptosporidium parvum,Giardia lambliaand helminth ova during a dry season with cold and warm periods. Samples of wastewater, reuse water for the irrigation of green areas and groundwater were collected on the campus of the National Autonomous University of Mexico. Physicochemical parameters were measured.Blastocystisspp. and its subtypes (ST) were identified using polymerase chain reaction (PCR) and indirect immunofluorescence (IFA);G. lambliaandC. parvumwere identified using IFA, and helminth ova were searched for using flotation/sedimentation and microscopic observation.BlastocystisST3,C. parvumandG. lambliawere detected in wastewater and reuse water, but onlyG. lambliawas detected in all of the samples of reuse water. Helminth ova were not detected. Statistical analysis showed a dependency between total dissolved solids and protozoa. Our data suggest thatG. lambliadetection can be performed as part of reuse water quality assessments for irrigation systems. [ABSTRACT FROM PUBLISHER]
- Published
- 2015
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