1. Degradation of aromatic compounds and degradative pathway of 4-nitrocatechol by Ochrobactrum sp. B2.
- Author
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Zhong, Qiuzan, Zhang, Haiyan, Bai, Wenqin, Li, Mei, Li, Baotong, and Qiu, Xinghui
- Subjects
BIODEGRADATION ,SOIL degradation ,PLASMIDS ,CHROMATOGRAPHIC analysis ,ORGANIC cyclic compounds ,BENZENE - Abstract
The potential capacity of a soil methyl parathion-degrading bacterium strain, Ochrobactrum sp. B2, for degrading various aromatic compounds were investigated. The results showed B2 was capable of degrading diverse aromatic compounds, but amino-substituted benzene compounds, at a concentration up to 100 mg L-1 in 4 days. B2 could use 4-nitrocatechol (4-NC) as a sole carbon and energy source with release of nitrite ion. The pathway for 4-NC degradation via 1,2,4-benzenetriol (BT) and hydroquinone (HQ) formation in B2 was proposed based on the identification and quantification of intermediates by gas chromatography-mass spectrometry (GC-MS), and high performance liquid chromatography (HPLC). Degradation studies carried out on a plasmid-cured derivative showed that the genes for 4-NC degradative pathway was plasmid-borne in B2, suggesting that B2 degrades both p-nitrophenol and 4-NC by enzymes encoded by genes on the same plasmid. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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