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This study evaluated five grape cultivars, viz. Cardinal, Prima, Victoria, Regal Seedless, and Sultanine, grown at four sites in the semi-arid Saïs region of Morocco. The research aimed to assess how the region’s climatic conditions influence grape growth and quality, to identify cultivars suitable for local golden raisin production. Pomological and physico-chemical characteristics were measured, including fruit size, seed and skin weight, juice yield, and soluble solids. The Sultanine cultivar proved advantageous, with the longest bunch length (27.96 cm), heaviest bunch weight (651.39 g), highest total soluble solids (25.50° Brix), and highest sugars (18.11%). Victoria, a white grape cultivar, had the largest berry diameter (2.15 cm) and highest ascorbic acid content (22.23 mg/100 g). The study highlights Sultanine’s exceptional quality and suitability for the Saïs region, making it an ideal candidate for high-quality local sultanas. There is potential to boost production and reduce Morocco’s dependence on imported golden raisins. [ABSTRACT FROM AUTHOR]

"Les raisins de la domination: Une histoire sociale de l'alcool en Tunisie à l'époque du Protectorat (1881–1956)" by Nessim Znaien is a book that explores the production, commercialization, and consumption of alcoholic beverages in Tunisia during the French protectorate era. The author analyzes the attitudes of public authorities towards alcohol consumption in a Muslim society and examines various sources such as administrative records, diplomatic archives, court records, and hospital archives. The book is divided into three parts, each focusing on different chronological periods, and provides insights into the development and changes in alcohol production and consumption during the protectorate era. The author also discusses the social and cultural implications of alcohol consumption in colonial Tunisia. [Extracted from the article]

In this study, the trace elements lead (Pb), arsenic (As), cadmium (Cd), nickel (Ni), copper (Cu), zinc (Zn) and iron (Fe) quantity have been investigated using graphite furnace atomic absorption spectrometry (GFAAS) and sulphur dioxide (SO2) residual by iodometric titration method in raisin samples in different regions of Iran. The results showed that the concentration of Cu, Zn and Fe were in the range of 0.053–0.212 mg/kg, 0.048–0.170 mg/kg and 0.541–0.843 mg/kg, respectively, whereas Cd, Ni, As and Pb concentrations in the samples were less than detection limit (LOD). The SO2 residual in raisin samples was between 30 and 843.33 mg/kg. The LOD of Cu, Fe, Zn, Cd, Pb, Ni, and As were 0.022 mg/kg, 0.5 mg/kg, 0.047 mg/kg, 0.04 mg/kg, 0.019 mg/kg, 0.024 mg/kg and 0.05 mg/kg, respectively. The Monte Carlo simulation results showed that the indexes of target hazard quotient (THQ) due to ingestion of SO2 residues estimated lower than the safe level (THQ < 1) in all samples, except in the Raisin-B and Raisin-D samples. Accordingly, to ensure consumer health and improve international trade, continuous control and adaptive regulation of sulphur dioxide residues and trace elements in dried fruit are recommended. [ABSTRACT FROM AUTHOR]

Including grape pomace in goat diets presents a potentially valuable strategy for enhancing the sustainability of goat farming and mitigating environmental risks. This work aimed to investigate the impact of dietary dried grape pomace (DGP) on milk yield, milk composition and milk fatty acid (FA) profile in goats. Gas chromatography was utilised to obtain an extensive profile of FA in milk fat. Dry matter intake, as well as milk yield and composition were not altered by dietary DGP. Despite the predominance of linoleic acid (LA, 18:2 cis-9,cis-12) in the lipids of DGP and the presence of phenolic compounds, its inclusion in the diet did not negatively affect the quantitatively main groups of FA. Furthermore, there was no significant impact on butyric, caproic, caprylic, and capric acids, the major 18:1 trans isomers, including vaccenic acid (18:1 trans-11), LA, α-linolenic acid (18:3 n-3), and the long chain polyunsaturated n-6 and n-3 FA. However, rumenic acid in milk fat was significantly reduced from 0.36 to 0.30 g/100 g of total fatty acid methyl esters. This study supports that up to 6%, DGP can be safely included in conventional dairy goat diets without compromising the production results or altering to great extent the milk FA profile. Milk changes due to dried grape pomace (DGP) consumption were assessed in goats. Milk yield increased without changes in milk composition following DGP consumption. Some minor FA contents were modified by DGP. DGP had no impact on most FA related to milk fat's nutritional properties. [ABSTRACT FROM AUTHOR]

This study was carried out to determine the effect of dietary flaxseed meal (FSM) supplemented with dried tomato pomace (DTP) and dried grape pomace (DGP) on performance, egg quality, biochemical parameters traits and antioxidant status of laying hens. Birds (1825 ± 87 g of body weight) were divided into 12 dietary groups with six replicates per group (eight birds per replicate), under a completely randomized design with factorial arrangement 2 × 3 × 2 consisted of two levels of DTP (0 and 15%), three FSM levels (0, 4 and 8%) and two levels of DGP (0 and 5%). As a result of this study, there were no significant differences in egg production and weight as well in feed conversion ratio (FCR) among treatments (p > 0.05). Feeding of DGP reduced significantly feed intake and egg mass when compared to control group (p < 0.05). There was no effect (p > 0.05) of dietary treatment on shell thickness and strength, shape index, Haugh unit and egg specific gravity. Hens consuming 15% DTP and 5% DGP revealed a significantly higher yolk color compared to the other dietary treatments (p < 0.05). Moreover, there was no difference among dietary treatments in terms of serum low-density lipoprotein cholesterol (LDL) and high-density lipoprotein cholesterol (HDL) cholesterol, atherogenic index, triglycerides, total cholesterol levels (p > 0.05). Serum antioxidant parameters as glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA), total superoxide dismutase (TSOD) and total antioxidant capacity (T-AOC) were not influenced by treatments (p > 0.05). Based on findings, FSM and DTP supplements did not significantly impact most of hens' performance indicators and egg quality parameters, whereas significant improvements were observed by feeding of 15% DTP and 5% DGP on egg traits, in particular on egg-yolk color that plays a key-role in consumer's choice. However, the supplementation of FSM and DTP or DGP even in laying hen diet is still controversial and further research is needed. [ABSTRACT FROM AUTHOR]

The re-release of golden lion tamarins (Leontopithecus rosalia), from zoos into the wild, is considered to be an ex situ conservation success story. However, zoo-born golden lion tamarins have lower survival rates than their wild-born offspring, potentially due to deficient foraging and locomotion 'survival skills' acquired in captivity. The current study aimed to evaluate whether a puzzle feeder suspended and baited with different items could promote increased foraging and general activity in tamarins living in a zoo setting. A pair of tamarins at Woodland Park Zoo received the puzzle feeder in three conditions in an ABCACB experimental reversal design over a period of three months: an empty feeder and feeders baited with either fruit (raisins) or insects (mealworms). Food type affected behavior; time spent climbing increased when the feeder contained insects compared to fruit. Subjects interacted with the baited puzzle feeder device significantly more than the empty feeder, and use was highest within the first 30 minutes of exposure. Our results suggest that the puzzle feeders plus food were effective in increasing foraging and general activity. [ABSTRACT FROM AUTHOR]

Drying is one of the best food preservation methods, especially for grapes. This study aimed to determine the fate of pesticides after grapes are being subjected to different drying methods, namely, sun-, oven-, shade-, and alkaline-drying, which are employed in raisin production. Pesticide residues were determined by gas chromatography–tandem mass spectrometry (GC–MS/MS). Washing before drying decreased the diazinon, ethion, phosalone, penconazole, and hexaconazole levels in the grape samples by 52.04%, 6.75%, 9.99%, 56.54%, and 20.97%, respectively. In the raisins, the concentration of all the pesticide residues increased based on wet weight, and this result was due to dehydration; by contrast, the concentrations of the pesticides decreased based on dry weight. The sun-drying method demonstrated the greatest efficiency in residue removal wherein it decreased the diazinon, ethion, phosalone, penconazole, and hexaconazole levels by 76.65%, 68.40%, 69.55%, 72.74%, and 82.19% (dry weight), respectively. On the contrary, the shade-drying method demonstrated the least efficiency in reducing the residue levels (19.57%–42.55%). The evaluation of the processing results showed pesticide residue level in the raisins prepared using the investigated drying methods was higher than the maximum residual limits (MRL). This finding indicates that it is necessary to consider the pre-harvest interval in pesticide application in grape. [ABSTRACT FROM AUTHOR]

Ochratoxin is a potent human toxin and suspected carcinogen metabolite generated by Penicillium spp. and Aspergillus spp. The current systematic meta-analysis review was conducted for the first time on the prevalence of ochratoxin A (OTA) in dried grapes and grape-derived products including sultana, currant, raisin, pekmez, and grape juice. All studies published up to December 2019 were searched in PubMed, Scopus, and Web of Science databases. The references of published papers were collected in order to investigate the prevalence of OTA in dried grapes and grape-derived products. The degree of heterogeneity between the studies was evaluated by the I2 heterogeneity statistic. The random effects model was used to calculate the pooled prevalence in subgroups such as country and type of grape products. The search retrieved 725 studies, 39 of which were included in this meta-analysis. According to reviewed reports, the global pooled prevalence of OTA was estimated as 42% (95% CI: 32%, 52%) for currants, 36% (95% CI: 20%, 52%) for grape juice, 39% (95% CI: 28%, 50%) for pekmez, 58% (95% CI: 48%, 68%) for raisins, and 27% (95% CI: 22%, 33%) for sultana. The rank of grape-based products based on mean concentration of OAT was in order of pekmez > raisin > sultana > currant > grape juice. The lowest and highest prevalence of OTA in different studied samples were related to grape juice and pekmez, respectively. The results of this meta-analysis can be useful in different risks management models, and can help health strategists and different industries to reduce the prevalence of OTA in grape-based products. [ABSTRACT FROM AUTHOR]

Grapevine cultivation is of historic importance arround the Mediterranean basin. The Spanish Levant is an area of notable grapevine diversity, where ancient varieties destined for producing wine, table grapes and raisins can still be found. For some of these neglected varieties, however, no complete description has ever been made; current legislation does not, therefore, allow their commercial use. The botanical characterization of old varieties is an important step toward their identification and can also help to clarify synonymies and homonymies, problems very commonly encountered with this crop. The present work provides ampelographic descriptions of seven genotypes for which microsatellite marker profiles were already available, and discusses the possible origins of their names, as well as instances of synonymy and homonymy. Seven accessions of ancient grapevine genotypes from the Spanish Levant were subjected to in situ ampelographic analysis following official methods. The characteristics of their leaves, bunches and berries were recorded. The ampelographic descriptions made in the present work provide insight into the history of cultivation of the examined genotypes and would pave the way for their inclusion in the Spanish national catalog, which would allow their commercial use. The information gathered on the possible origin of these genotypes' names, and instances of synonymy and homonymy, enriches our knowledge of this recovered germplasm. The present results contribute toward our understanding of Europe's grapevine genetic diversity, and highlight the need to conserve it. [ABSTRACT FROM AUTHOR]

Growing interests in natural and organic meat products have led to increasing application of fruits and vegetables, containing antimicrobial and antioxidant components as natural preservatives, in processed meats. The aim of this study was assessed the utilization of raisin as a natural preservative in goat meat (chevon), and thereby the impacts on quality parameters of restructured chevon jerky and its stability were evaluated. Incorporating 15% raisin increased (P <.01) darkness (lower CIE L*), but decreased (P <.01) redness and yellowness (lower CIE a* and b*) of chevon jerky. Furthermore, all instrumental color values of experimental jerky decreased (P <.01) over a 10-week storage period. Compared with jerky prepared with or without sodium nitrite (NaNO2), jerky prepared with raisin had significantly higher shear values, but lower water activity, pH and aerobic microbial counts. Furthermore, as the storage time progressed, pH values decreased (P <.01) in all jerky types, whereas the aerobic counts significantly increased. Either raisin or NaNO2 added to jerky decreased (P <.05) thiobarbituric acid reactive substances (TBARS) values, which were not changed over the 10-week storage. Raisin jerky scored lower (P <.01) in the sensory properties of color and overall acceptability than the cured jerky. However, texture and flavor sensory characteristics of raisin jerky were not differentiated from the cured jerky by the sensory panel. Consequently, raisin has potential to use as a natural preservative in processed meats and it may be possible to substitute raisins for nitrites in processed meats. [ABSTRACT FROM AUTHOR]

This study investigated the reduction of hexavalent chromium (Cr(VI)) with sulfur dioxide (SO2) and adsorption of Cr(VI) onto dried grape pulp carbonized with sulfuric acid. Cr(VI) reduction capacities of SO2 were determined. The filtrate was titrated with NaOH solution after shaking and filtering the carbonized material to retain unreacted sulfuric acid (H2SO4). Simple washing recovered 25–38% of the experimental acid at low concentrations. The carbonized material was washed twice with distilled water and then dried at 105 °C and weighed. The carbonized material had a yield of 56.6% (grape pulp/sulfuric acid ratios of 1:2), and the lower the H2SO4 content, the better the yield, suggesting that the higher the acid content, the lower the Cr(VI) content per unit grape pulp. Cr(VI) reduction capacities were 219.5, 195.3, and 190.9 mg Cr(VI)/g-H2SO4 for the grape pulp/sulfuric acid ratios of 1:1, 1:2, and 1:3, respectively. Novelty statement: A carbonaceous material was obtained from grape pulp by carbonizing with concentrated sulfuric acid. The main objective of this study was to evaluate gas, liquid, and solid products or co-products obtained during carbonization process for hexavalent chromium treatment in aqueous solutions. In this context, (a) hexavalent chromium reduction capability of the gas evolved during carbonization was determined, (b) characterization of unreacted acid recovered by washing the carbonized product left after carbonization step was done, (c) carbonaceous adsorbent obtained was characterized and (d) hexavalent chromium adsorption characteristics of carbonaceous material obtained was determined. Reduction and adsorption mechanisms of hexavalent chromium were investigated. A waste recycling method was proposed. The effects of sulfuric acid on carbonization were assessed. The structures and chemical compositions of a carbonized material were evaluated. The carbonized material is a cost-effective porous adsorbent for a clean environment. [ABSTRACT FROM AUTHOR]

The sensory characteristics, texture profiles, and volatile compounds were investigated for grape raisins dehydrated by pulsed vacuum drying (PVD) and shade drying (SD) from three varieties (Thompson Seedless, Flame Seedless, and Munage). Results indicated that raisins dried from PVD were less brown, possessed stronger fruity aroma and flavor. Texture profile analysis revealed that samples dried by PVD displayed lower hardness and higher springiness, cohesiveness and resilience than the SD ones. In terms of the volatiles analysis, 34 compounds were identified including 7 alcohols, 7 aldehydes, 5 acids, 3 esters, 2 ketones, 5 terpenoids, 1 pyrazine, 2 furfurals, and 2 aromatic compounds. Raisins dried by PVD displayed higher relative content of esters and terpenoids, which contributes to the floral and fruity aroma. The drying methods were well differentiated according to the principal component analysis. The current findings indicate that PVD is an alternative promising technology for production of high-quality raisins. [ABSTRACT FROM AUTHOR]

Jerez de la Frontera and its wines enjoy an unquestionable reputation. The expansion of wine production and consumption in the Early Modern period was made possible by developments that began at the end of the Middle Ages, on which this paper focuses. Our aim is to analyze two products of the vine, raisins and wine, which had distinctive identifying characteristics in the period studied. It was in the fifteenth century that Jerez de la Frontera, according to its socioeconomic situation, became the second most important city of the Kingdom of Seville, due in part to the production of wine and the thriving community of merchants, as well as the activities associated with its pier (called El Portal) and the port at Bahía de Cádiz. The present study centers on the period from the fifteenth century through the turn of the sixteenth to examine the place of raisins and wines from Jerez in commercial circuits along the Mediterranean Sea and the north–south routes of the Atlantic Ocean. [ABSTRACT FROM AUTHOR]

The availability of powdery mildew-resistant raisin accessions will lower grower production costs and enhance the environment through reduced fungicide use. To achieve this objective, backcrossing has been employed with Vitis romanetii as the source of non-race-specific powdery mildew resistance. Initial crosses of powdery mildew-resistant F1 hybrid B36-45 with seeded raisin cultivars 'Rangspray' and 'Raisin de Palestine' yielded seedless powdery mildew-resistant first-generation backcross selections C87-41 and C87-106, which were used to develop second-generation backcross populations. Principal component analyses consistently identified 'wrinkle,' 'meatiness,' 'product attractiveness' and 'skin persistence' as being the quality characteristics most important in discriminating among powdery mildew-resistant raisin accessions and commercial raisin cultivars. Raisin quality ratings were much improved across most evaluated characters in second-generation backcross families as compared with parental powdery mildew- resistant accessions C87-41 and C87-106. After two generations of backcrossing, powdery mildew-resistant raisin selections were identified with product quality characteristics similar to those of commercial raisin cultivars. [ABSTRACT FROM AUTHOR]

Processing and conservation of grapes by suitable techniques has been a major challenging issue for a long time. Optimization of drying and pretreatment operations of this fruit have been extensively studied. However, in order to achieve the production of high-quality raisins and reach consumers' acceptance, special attention for quality attributes should be taken into account. Quality characteristics of grapes such as color, texture, vitamins, phytochemicals, aroma profile and microbial stability are of paramount importance since they could vary throughout the dehydration procedure and would directly determine quality perception and consumer choice. This paper presents a comprehensive review of the physicochemical, nutritional and microbiological characteristics of dried grapes as affected by the drying process. In addition, it investigates the changes of different grapes quality attributes (mainly nutritional and aromatic proprieties) during processing, which enables professionals and scientists to better choose and optimize grape processing to deliver the highest raisin quality to consumers. [ABSTRACT FROM AUTHOR]

We determined the effect of different storage systems and packaging on the quality of ‘Sultana Seedless’ raisins. The fruit were packed in plastic boxes and kept under controlled atmosphere (1% O2and 3% CO2) at 0°C and 75–85% RH for 12 months (CA), packed in vacuum bags and kept under normal atmosphere (21% O2–0.03% CO2) (NA) and packed in thin plastic bags + carton boxes and kept under ambient condition (semi-refrigeration), at 0°C and 90% ± 5 RH for 12 months (AC). Fruit kept under CA or NA had acceptable fruit quality in terms of general appearance and taste for 10 months, whereas fruit quality was unacceptable at that time under AC. Fruit under NA had less weight loss than fruit under the other conditions. Differences in skin colour were relatively small, apart from the h° values. Concentrations of ochratoxin A were generally higher with AC. External appearance and taste were better with CA and NA. As a result, raisins can be stored for 10 months with good quality in vacuum package in NA (0°C and 90% ± 5 RH) and CA (1% O2–3% CO2) at 0°C and 75–85% RH. [ABSTRACT FROM PUBLISHER]

Objectives:Although grape has been recently the topic of many investigations,Maviz(a kind of dried one) has remained neglected. The aim of this study was to assess anti-Alzheimer activity ofMaviz. Methods:To reach this goal, total phenolic content (TPC) of ethanolic (Eth) and aqueous (Aq) extracts were determined and radical scavenging activity was assayed by 2,2-diphenyl-1-picrylhydrazyl. Chemical compositions of each extract were also determined via GC-Mass. Behavioral changes were studied via passive avoidance and Morris water maze in Aβ-induced model of Alzheimer's disease. Catalase (CAT) and superoxide dismutase (SOD) determination were also done on rats’ hippocampus. Results:The results showed that seed Eth extract has a high level of TPC and radical scavenging activity. However, this extract had surprisingly no effect on memory and CAT and SOD activities. In contrast, fruit Aq and Eth extracts (containing furfurals as major compounds) inhibited memory impairment (P < 0.001) and elevated brain levels of CAT and SOD(P < 0.05). Conclusion:It seems thatMaviznon-phenolic compounds-most probably 5-hydroxymethylfurfural and other similar derivatives-are responsible for these actions. [ABSTRACT FROM AUTHOR]

Magnetic resonance imaging (MRI) is a non-invasive imaging technique that can visualise samples’ interior by using the signal coming from mobile protons. The aim of this study was to examine the effects of oil content and peanut/raisin addition on cake quality and to illustrate the power of MRI in analysis of moisture and oil distribution. For this purpose, MR images were acquired with a spin echo sequence and relaxation times T1and T2, and moisture content and firmness of cakes were measured. High oil cakes (HOC) had higher moisture content and lower firmness than low oil cakes (LOC). However, addition of raisin/peanut did not affect the firmness of cakes significantly. In MR images, HOC cake crumb, owing to its higher oil content, displayed larger signal intensities. Signal acquired from different slices demonstrated an increase in moisture content from crust to centre of the cakes. Peanut and raisin signals were suppressed in fat and water suppression sequences, respectively. Significant correlation between transverse relaxation time (T2a) and oil content (R2= 0.99) was found. Moreover, longitudinal relaxation time (T1) was found to be strongly correlated with moisture content (R2= 0.99). The results demonstrated MRI’s power as an accurate and non-invasive analysis method in baking. [ABSTRACT FROM AUTHOR]

The biochemical characterization of polyphenol oxidase (PPO) in the white grape Bianchetta Trevigiana is addressed in this research to fulfil the requirement for raisin varieties with high concentrations of phenolic compounds and low PPO activity not only to avoid enzymatic browning but also for maintaining the nutritional value of raisins. Spectrophotometric assays and electrophoresis were used to study this ancient variety exhibiting low PPO activity (only cathecolase activity) which reached at the low pH value of grape berry and was about 15% of the maximum enzymatic activity measured. In addition, the visualization of the active isoforms of grape PPO after 2-DE separation of proteins was a key step for further studies using MALDI-TOF-MS and bioinformatic tools. Based on the thermal inactivation profiles, heating treatment at 70°C may be considered a way to inhibit Bianchetta Trevigiana PPO activity by preserving also its high phenolic content. Therefore PPO characterization may be proposed as a marker for identifying potential high value raisin varieties. [ABSTRACT FROM PUBLISHER]

During 2012–2014, 170 samples of export quality raisins were collected from different vendors in Pakistan. The collected samples were analysed for the presence of aflatoxins (AFs) and Ochratoxin A (OTA) contamination using high-performance liquid chromatography technique. The limit of detection and limit of quantification of AFs/OTA were 0.12/0.10 and 0.36/0.30 µg kg−1, respectively. Only 5% of the samples were contaminated with AFs, ranging 0.15–2.58 µg kg−1with a mean of 0.05 ± 0.26 µg kg−1. None of the raisin samples exhibited AFs contamination above the maximum limit (ML = 4 µg kg−1) as set by the European Union (EU). About 72% of the samples were contaminated with OTA, ranging 0.14–12.75 µg kg−1with a mean of 2.10 ± 1.9 µg kg−1. However, in 95.3% of the tested samples, OTA level was lower than the ML of 10 µg kg−1as regulated by the EU. Apparently, a strict and continuous monitoring plan, including regulatory limits, improves food safety and quality for all types of commodities. [ABSTRACT FROM PUBLISHER]

Four raisin (Vitis viniferaL.) varieties, Chriha, Razeki, Assli, and Meski, were evaluated for total phenolic content, total o-diphenol content, total flavonoid content, total condensed tannin, total carotenoid content, and total anthocyanin content. Antioxidant potential was assessed by three assays: 2,2-Diphenyl-1-Picrylhydrazyl and 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical scavenging capacity and ferric reducing power. Individual phenolic profiles were determined by high-performance liquid chromatography. The results revealed that the four raisin varieties had considerable phenolic content and antioxidant activity. Chriha had the highest total phenolic content (534.2 mg/g dry weight) while Meski had high total condensed tannin (208.6 mg CEQ/g dry weight), TAC (137 mg/100 g dry weight), total o-diphenol content (115.8 mg/g dry weight), total flavonoid content (93 mg CEQ/g dry weight), and total carotenoid content (33 mg/100 g dry weight). There were significant differences in phenolic content among the four varieties (p< 0.05). Meski had the highest 2,2-Diphenyl-1-Picrylhydrazyl scavenging capacity, while Chriha had adequate reducing power and 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid scavenging capacity. The individual phenolic compounds (2.96–6.54 mg/g dry weight) were variety-dependent. [ABSTRACT FROM AUTHOR]

This study analyzed the aromatic composition and established the soluble carbohydrate profiles of the Chriha, Razeki, Assli, and Meski varieties of Tunisian raisin (Vitis viniferaL.). A total of 80 compounds obtained by headspace solid phase micro-extraction were detected by gas chromatography-mass spectrometry. Non-terpene hydrocarbon derivatives were the major compounds of the Chriha and Assli varieties (35.8 and 26.3%, respectively). The Razeki variety accumulated 25.5%. apocarotenoids. Oxygenated non-terpene derivatives, including esters, alcohols, aldehydes, acids, ketones, and others were the most abundant (57.5%) in the Assli variety. The presence and amounts of volatiles were different among varieties and might be responsible, in part, for the aroma and taste of raisins. The concentration of total sugars was highest in the Razeki variety and lowest in the Chriha variety. The content of individual sugars differed quantitatively among the varieties in this study. The Razeki variety had the best fruit quality with higher contents of individual and total sugars compared to the other three varieties. [ABSTRACT FROM AUTHOR]

The occurrence of ochratoxin A (OTA) in dried grapes was surveyed in this study. Sixty-six samples of dried grapes (40 currants, 16 sultanas and 10 raisins) were collected from dried grapes factories in Hamadan province, Iran, from October 2012 to March 2013. High-performance liquid chromatography (HPLC) was used to determine OTA in these samples. OTA was detected in 23 (57.5%) currants, 10 (62.5%) sultanas and 6 (60%) raisins samples. Levels in five samples exceeded the Institute of Standards and Industrial Research of Iran (ISIRI) maximum level of 5 µg/kg. However, OTA content in none of the samples exceeded the maximum limit prescribed in the European Union (EU) regulations, which is 10 µg/kg. The obtained data contribute to information on OTA levels in Iranian dried grapes. [ABSTRACT FROM PUBLISHER]

Just as the type and duration of physical activity can have variable effects on the glucose levels and other cardiometabolic parameters among patients with type 2 diabetes mellitus (T2DM), so can the types of foods have variable effects as well. This 12-week randomized study of 51 study participants evaluated the impact of routine consumption of dark raisins versus alternative processed snacks on glucose levels and other cardiovascular risk factors among patients with type T2DM. In this study, compared to alternative processed snacks, those who consumed raisins had a significant 23% reduction in postprandial glucose levels (P= 0.024). Also compared to snacks, those who consumed raisins had a 19% reduction in fasting glucose and 0.12% reduction in hemoglobin A1c, although these latter findings did not achieve statistical significance. Regarding blood pressure, compared to alternative processed snacks, those who consumed raisins had a significant 8.7 mmHg reduction in systolic blood pressure (P= 0.035) (7.5% [P= 0.031]) but did not experience a significant reduction in diastolic blood pressure. Compared to alternative processed snacks, those who consumed raisins did not have a significant improvement in body weight, body mass index, waist circumference, fasting insulin, homeostatic model assessment of insulin resistance, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol (HDL), triglyceride, or non-HDL cholesterol levels. Overall, these data support raisins as a healthy alternative compare to processed snacks in patients with T2DM. [ABSTRACT FROM AUTHOR]

‘Thompson Seedless’ grapes grafted on five rootstocks and own-rooted vines were evaluated for yield, fruit composition, and raisin recovery percentage. Biochemical constituents were analyzed in fresh grapes before drying into raisins and also in raisins. Among different rootstocks, raisin recovery was highest in ‘Thompson Seedless’ grapes grafted on 110 R followed by those on ‘Dogridge’, 1103 P and 99 R. Significant differences were observed in total proteins, reducing sugars, phenol contents, and polyphenol oxidase (PPO) activity in both fresh grapes and raisins made from all rootstock scion combinations. Increase in the content of proteins and reducing sugars was observed in raisins compared to fresh grapes. However, there was a reduction in phenolic concentrations in raisins compared to fresh grapes. PPO activity was highest in ‘Thompson Seedless’ grapes grafted on ‘Dogridge’, while it was least in 110 R. [ABSTRACT FROM AUTHOR]

Despite the existing vertically coordinated (specifications contracting) relationship between the raisin producers from Eksteenskuil and their buyer, the farmers still face relatively high transaction costs. High transaction costs may cause the farmers to lose their share in the highly profitable fair-trade market for raisins and consequently decrease the contribution of raisin production to the livelihoods of the farmers from Eksteenskuil. The aim of this paper is to investigate whether a higher degree of vertical coordination will be more appropriate to economise the transaction costs faced by the farmers in order to allow them to continue benefitting from the fair-trade initiative. Based on the application of the frameworks of Mahoney (1992) and Peterson et al. (2001), a higher degree of vertical coordination in the form of a relation-based strategic alliance will be more appropriate than specifications contracting. The complementarity between the farmers and their buyer, created by the fair- trade initiative, contribute to the viability of a more vertically coordinated strategy. Similar incentives for private sector-buyers to procure from emerging farmers may create a similar degree of complementarity, and hence an incentive for such buyers to enter into vertically coordinated relationships with emerging farmers. [ABSTRACT FROM PUBLISHER]

The presence of ochratoxin A (OTA), fumonisin B2(FB2) and black aspergilli in raisins from Western Greece regions (Messinia, Corinthia, Achaia, Ilia and Zante Island) was investigated in relation to the different geographic and climatic conditions in the 2011 growing season. The biseriate speciesAspergillus niger“aggregate” andA. carbonariuswere mainly identified. The population ofA. niger“aggregate” species occurred in all raisin samples at colony-forming units (CFU) concentrations significantly higher (mean 2.2 × 105CFU g−1homogenate) than those ofA. carbonariuspopulation (mean 4.9 × 103CFU g−1homogenate), which occurred in 80% of the raisin samples. OTA was found in 73% of the samples at levels ranging from 0.1 µg kg−1to 98.2 µg kg−1, with the highest level occurring in a raisin sample from Ilia that also contained the highest level ofA. carbonarius. The European Union legal limit for OTA was exceeded in 15% of the raisin samples. FB2was found in 29% of the raisin samples at levels ranging from 7.1 µg kg−1to 25.5 µg kg−1, with 20% of the samples co-occurring with OTA. Principal-component analysis was applied to levels of mycotoxins, fungal contamination, geographical data and environmental conditions recorded in the harvesting (August) or drying (September) period. Principal-component analysis clearly indicated a good direct correlation of rainfall and relative humidity with OTA andA. carbonariuscontamination. A lack of clustering was observed whenA. nigerand FB2contamination were considered. This is the first report on the co-occurrence of the mycotoxins OTA and FB2in dried vine fruits from Greece. [ABSTRACT FROM AUTHOR]

To determine the genetic basis for loss of fumonisin B2 (FB2) biosynthesis in FB2-nonproducing Aspergillus niger and A. awamori strains, we developed multiplex PCR primer sets to amplify fragments of eight fumonisin biosynthetic pathway (fum) genes. Fragments of all eight fum genes were amplified from FB2-producing A. niger and A. awamori strains; from FB2-nonproducing strains four amplification patterns arose in which one or more fum gene fragments were absent. Southern hybridization analysis of strains yielding patterns 2 and 3 confirmed that loss of FB2 production in A. awamori is associated with gene deletions within the fumonisin biosynthetic gene cluster. In addition, we observed a fifth multiplex amplification pattern in which all eight fum gene fragments appeared. Reverse transcription-PCR analysis of strains yielding pattern 5 showed that the expression of at least one fum gene was reduced relative to expression in FB2-producing A. niger. This suggests that in these strains loss of FB2 production is a result of structural or regulatory mutations that alter gene expression or function. These results demonstrate a diversity of genotypes within FB2-nonproducing A. niger and A. awamori populations and provide tools useful for identifying certain non-toxigenic strains for industrial or ecological applications. [ABSTRACT FROM AUTHOR]

Background: We examined the metabolic, performance and gastrointestinal (GI) effects of supplementation with a natural food product (raisins) compared to a commercial product (sport chews). Methods: Eleven male (29.3 ± 7.9 yrs; mean and SD) runners completed three randomized trials (raisins, chews and water only) separated by seven days. Each trial consisted of 80-min (75%VO2max) treadmill running followed by a 5-km time trial (TT). Heart rate (HR), respiratory exchange ratio (RER), blood lactate, serum free fatty acids (FFA), glycerol and insulin, plasma glucose and creatine kinase, GI symptoms and rating of perceived exertion (RPE) were recorded every 20-min. We employed a within-subject two-way analysis of variance (ANOVA) for repeated measures with a Fisher’s post hoc analysis to determine significant differences. Results: VO2, HR, lactate, glycerol and RPE did not differ due to treatment. Average plasma glucose was maintained at resting levels (5.3 ± 0.4 mmol•L-1) during the sub-maximal exercise bout (5.9 ± 0.6, 5.7 ± 0.6 and 5.5 ± 0.5 mmol•L-1for chews, raisins and water respectively), and was significantly higher with chews than water only. RER and % of non-protein macronutrient oxidation derived from carbohydrate was highest with chews, followed by raisins and water was the lowest (74.4 ± 6.4, 70.0 ± 7.0 and 65.1 ± 8.7% for chews, raisins and water respectively) during the submaximal exercise period. Serum FFA was higher in the water treatment versus both raisins and chews at 80 min of sub-maximal exercise. Serum insulin was higher with the chews than both raisins and water (5.1 ± 2.0, 3.1 ± 0.8, 1.9 ± 0.6 uU•ml-1for chews, raisins and water respectively). Plasma creatine kinase, corrected for baseline values, for the last 40 min of the sub-maximal exercise bout, was higher with raisins compared to other treatments. The TT was faster for both carbohydrate supplements (20.6 ± 2.6, 20.7 ± 2.5, 21.6 ± 2.7 min for raisin, chews and water respectively). GI disturbance was mild for all treatments. Conclusion: Raisins and chews promoted higher carbohydrate oxidation and improved running performance compared to water only. Running performance was similar between the raisins and chews, with no significant GI differences. [ABSTRACT FROM AUTHOR]

The aim of this research was to determine the changes in chemical and microbiological qualities of ready-to-eat intermediate-moisture raisins during storage. Raisins were immersed into hot (98±1°C) glucose solutions (10% w/v and 20% w/v) containing 1% (w/v) potassium sorbate to inhibit mould and yeast growth. Raisins were kept in glucose solutions until the moisture content became 20%, 25% and 30%, respectively. The reconstituted raisins were then stored at ambient temperature (23±3°C) and 50-60% relative humidity for 8 months in polyethylene/polyamide (PE/PA) packaging material. The changes of moisture content, water activity, pH values, total titratable acidity, total sugar contents, Hunter L, a and b color values, total mesophylic aerobic bacteria counts, and mould and yeast counts were determined during storage at 2-month intervals. The results of chemical analysis showed that changes in the total titratable acidity, total mesophylic aerobic bacteria, and mould and yeast counts of the products were increased significantly (P<0.05). However, the pH values, total sugar contents, and Hunter L, a, and b color values were decreased during the storage period (P<0.05). [ABSTRACT FROM AUTHOR]

Currants and Sultanas (Vitis vinifera L.) are dried vine products produced in Greece and used broadly in the Mediterranean diet. We aimed to investigate the gastric cancer preventive activity of methanol extracts obtained from currants from three different origins in Greece (Vostizza, Nemea, and Messinia) as well as methanol extracts obtained from Sultanas cultivated in the island of Crete as to inhibition of cell proliferation, induction of apoptosis, and inhibition of inflammation. All extracts from 500 μg dried raisins studied suppressed cell proliferation, significantly those obtained from Sultanas from Crete and currants from Nemea. Flow cytometric analysis of Annexin-V labeled cells indicated that Cretan Sultana, Nemea, and Messinia currants at 500 μg dried product/ml medium significantly induced cell death. All extracts from 500 μg dried raisins statistically decreased protein and mRNA levels of ICAM-1 in TNF-alpha stimulated cells. Measurement of IL-8 protein levels and quantification for IL-8 mRNA showed no significant decrease. These results indicate that the methanol extracts from currants, rich in phenolic compounds, exhibit cancer preventive efficacy by limiting cell proliferation, inducing cell death, and suppressing ICAM-1 levels in AGS cells. [ABSTRACT FROM AUTHOR]

In order to discourage the use of chemicals in raisin processing, the effect of microwave and pulsed electric field (PEF) pretreatments on the drying rate and other quality parameters like color, total soluble solids, bulk density, appearance, and market quality were compared with that of chemically pretreated raisins dried at 65°C. The untreated and pretreated samples had a statistically significant difference in drying rate (P < 0.05). The drying rate of chemically pretreated raisins was the highest when compared to others. The results showed that the PEF and microwave-treated samples had a significantly high Total Soluble Solids (TSS), along with good appearance and market quality. [ABSTRACT FROM AUTHOR]

Botanicals are used in many countries for medicinal and general health-promoting purposes. Numerous natural occurrences of mycotoxins in botanicals and dried fruits have been reported. Aflatoxins or ochratoxin A (OTA) have been found in botanicals such as ginseng, ginger, liquorice, turmeric, and kava-kava in the USA, Spain, Argentina, India, and some other countries, while fumonisins have been found in medicinal wild plants in South Africa and in herbal tea and medicinal plants in Turkey. Zearalenone was identified in ginseng root. Dried fruits can be contaminated with aflatoxins, OTA, kojic acid, and, occasionally, with patulin or zearalenone. One main area of concern is aflatoxins in dried figs; bright greenish yellow fluorescence under ultraviolet light is associated with aflatoxin contamination. OTA in dried vine fruits (raisins, sultanas, and currants) is another concern. There are also reports of aflatoxins in raisins and OTA in dried figs, apricots, dried plums (prunes), dates, and quince. Maximum permitted levels in the European Union include 4 µg kg-1 for total aflatoxins in dried fruit intended for direct consumption and 10 µg kg-1 for OTA in dried vine fruit. This review discusses the occurrence of mycotoxins in botanicals and dried fruits and analytical issues such as sampling, sample preparation, and methods for analysis. Fungal contamination of these products, the influence of sorting, storage, and processing, and prevention are also considered. [ABSTRACT FROM AUTHOR]

Lead and copper levels in various types of Turkish raisins, collected from the most important production centers, were determined by electrothermal atomic absorption spectrometry. Samples were principally the products of 2005; however, two different and important raisin types produced between 2003 and 2006 were also analyzed. To investigate the source of pollution, the lead and copper content of all samples were separately determined after successive treatment with water, then with acetone and, finally, complete decomposition in a HNO3/H2SO4/H2O2 mixture. Metal levels in raisin seeds were also determined. The results were statistically evaluated, indicating that the raisins taken from different regions and years contained a mean (range) of 0.056 (0.012-0.359) mg kg-1 lead and 2.542 (0.770-4.706) mg kg-1 copper. Lead concentrations in Turkish raisins were significantly lower than those found in a previous study. [ABSTRACT FROM AUTHOR]

The drying of grapes is a more complex process compared to the dehydration of other agricultural materials due to the necessity both of a pretreatment operation prior to drying and further processing operations after drying. To date, most researchers have concentrated on pretreatment and drying where the primary effects of pretreatment on drying have been evaluated. It seems appropriate that a review should now be presented to critically examine all the corresponding published data in the literature and to compare the conditions used in these studies in order to highlight the grape drying process by considering the important aspects of both the pretreatment and post-drying operations. The result of this study will be useful in identifying the future research directions needed for grape drying and also to facilitate the development of an analytical framework for the design of novel drying equipment. [ABSTRACT FROM AUTHOR]

One hundred samples of dried fruits (20 dried raisins, 20 walnuts, 20 peanuts, 20 dried figs and 20 pistachios) and 20 samples of rice purchased from retail shops in the Rabat and Salé area in Morocco were analysed for ochratoxin A (OTA) by immunoaffinity clean-up (IAC) and liquid chromatography (LC) with fluorescence detection. The limit of quantification (LOQ) (S/N = 10:1) of OTA was 0.02 ng g-1 in rice, 0.03 ng g-1 in pistachio, peanut and walnut, and 0.03 ng g-1 in dried raisins and dried figs. The incidences of occurrence of OTA in dried raisins, walnuts, peanuts, dried figs and rice were 30, 35, 25, 65 and 90%, respectively. Analytical results showed that pistachio samples contained no detectable OTA, but concentrations ranged from 0.02 ± 0.01 to 32.4 ± 2.10 ng g-1 in rice, from 0.10 ± 0.05 to 2.36 ± 0.75 in peanut, from 0.03 ± 0.01 to 1.42 ± 0.45 in dried figs, from 0.05 ± 0.02 to 4.95 ± 0.02 in dried raisins, and from 0.04 ± 0.01 to 0.23 ± 0.05 in walnuts. The results also showed that 15% of the total number of rice samples analysed exceeded the 2002 regulatory limit set by European Union regulations for cereals. This is the first report on the occurrence of OTA in dried fruits and rice available in Morocco. [ABSTRACT FROM AUTHOR]

The effect of pH (2–10) on growth and ochratoxin A (OTA) production by 12 Aspergillus niger aggregate strains was studied in two culture media: Czapek yeast autolysate agar (CYA) and yeast extract sucrose agar (YES), over 30 days. The strains were selected to include different sources, different reported abilities to produce OTA and different ITS-5.8S rDNA RFLP patterns. YES was a better culture medium than CYA for OTA production. In this medium, OTA was produced from pH 2 or 3 to 10 depending on the strain. The results show the ability of A. niger aggregate strains not only to grow, but also to produce OTA over a wide pH range. The results will lead to a better understanding of the role of A. niger aggregate strains in the OTA contamination of several food commodities. [ABSTRACT FROM AUTHOR]

Raisin juice concentrate is a natural sweetener in syrup or paste form and it is produced from second-grade dry raisins by leaching them with water. Dried raisin juice, although is easier to handle and has more potential applications than the syrup, is not available in the market. In the present study, raisin juice powder was produced with a lab-scale spray dryer. The problem of stickiness in the drying chamber was overcome through the use of 21 DE, 12 DE, and 6 DE maltodextrins as drying aid agents. For each type of maltodextrin, the dryer operating conditions and the minimum concentration of maltodextrin in the feed, necessary for successful powder production, were determined. The maximum ratio of (raisin juice solids)/(maltodextrin solids) achieved was 67/33 and was made possible with the use of 6 DE maltodextrin. The inlet and outlet drying air temperatures were 110 and 77°C respectively, while the feed contained 40% w/w total solids. The physical and sensory properties of all powders produced were determined and found to be satisfactory, the only exception being their high hygroscopicity. [ABSTRACT FROM AUTHOR]

Effects of ohmic pretreatment on the drying rates of seedless red grapes and adsorption isotherms of the raisins produced were investigated. The treated grape samples were heated in a medium containing 2% citric acid to a final medium temperature of 60°C using an alternating current at 14 V/cm held strength and 30 Hz, 60 Hz, and 7.5 kHz Frequencies. Results of this study reveal that the drying rate of the grapes was significantly increased by the ohmic pretreatment. especially at low electrical frequencies. The effect of the ohmic pretreatment on equilibrium moisture content ot the raisins produced was evident at 0.75 or higher water activities hut there was no or limited effect at low to moderate water activities. [ABSTRACT FROM AUTHOR]

Between 1998 and 2000, 151 samples of raisins and sultanas and two samples of currants were collected from retail outlets across Canada and analysed for ochratoxin A. Samples were extracted with methanol-sodium bicarbonate, and the extracts were cleaned-up by immunoaffinity column chromatography. Ochratoxin A was quantified by liquid chromatography with fluorescence detection. The minimum quantifiable level was 0.1 ng g-1. Ochratoxin A was present, above the minimum quantifiable level, in 67 (79%) of 85 samples of raisins, in 39 (59%) of 66 samples of sultanas, and in both samples of currants. The overall mean level of ochratoxin A was 1.8 ng g-1 in both the raisins and sultanas, and 2.8 ng g-1 in the currants. [ABSTRACT FROM AUTHOR]

A basic extraction procedure for analysis of ochratoxin A (OTA) in currants and raisins is described, as well as the occurrence of OTA and a control of heterogeneity of the toxin in samples bought for two small marketing surveys 1999/2000 and 2001/02. Most samples in the surveys were divided into two subsamples that were individually prepared as slurries and analysed separately. The limit of quantification for the method was estimated as 0.1 μg kg -1 and recoveries of 85, 90 and 115% were achieved in recovery experiments at 10, 5 and 0.1 μg kg -1 , respectively. Of all 118 subsamples analysed in the surveys, 96 (84%) contained ochratoxin A at levels above the quantification level and five samples (4%) contained more than the European Community legislation of 10 μg kg -1 . The OTA concentrations found in the first survey were in the range < 0.1-19.0 μg kg -1 with a median concentration of 0.9 μg kg -1 . In the 2001/02 study, the range was < 0.1-34.6 μg kg -1 with a median of 0.2 μg kg -1 . Big differences were often achieved between individual subsamples of the original sample, which indicate a wide heterogeneous distribution of the toxin. Data from the repeatability test as well as recovery experiments from the same slurries showed that preparation of slurries as described here seemed to give a homogeneous and representative sample. The extraction with the basic sodium bicarbonate-methanol mixture used in the surveys gave similar or somewhat higher OTA values on some samples tested in a comparison with a weak phosphoric acid water-methanol extraction mixture. [ABSTRACT FROM AUTHOR]

The current use of sulfur dioxide (SO2) fumigation to bleach Sultana raisins has a number of limitations including poor absorption into the product and air pollution problems. A study was conducted to improve the sulfitation process by use of SO2 solutions as an alternative. A factorial design was used to evaluate the effect of concentration, temperature and raisin/solution ratio on the absorption of SO2 by raisins. It was found that the most important variables were concentration and temperature of the used solutions. The amount of SO2 absorbed by the raisins was time dependent, but the absorption rate declined after the first 15 min of contact. The use of SO2 solutions was found to be more efficient than fumigation with raisins retaining from 9–21% of applied SO2 in solution compared to 3–5% of SO2 applied as a gas. Higher final SO2 tissue concentrations could be achieved in the liquid application method compared to the gas method. There were no negative consequences of the proposed solution-based system with respect to post-treatment drying time and the storability of the raisins. [ABSTRACT FROM AUTHOR]

Residues of the synthetic pyrethroid insecticide cypermethrin in fresh grapes of cv. Thomson Seedless and in raisins were studied. The commercial formulation Cymbush® 10% EC was applied according to the label onto the typical seedless variety and on a seed-producing clone. Samples were collected at time zero and at 15 or 21 days post-application. The raisins were produced either by submersion for 3 min in an aqueous solution of 3% potassium carbonate and 1% ethyl oleate, and dried for 15 days under direct sunlight or were dried without chemical processing. A gas chromatographic method was used for the analysis of residues. The residues in the grapes at the recommended preharvest interval (15 days) ranged from 0.23 to 0.41 mg kg[sup -1], whereas those in raisins ranged from 0.25 to 0.46 mg kg[sup -1]. [ABSTRACT FROM AUTHOR]

Graphite-furnace atomic absorption spectrometric analysis of raisins imported in 1993-95 from different countries into Canada showed that raisins from Turkey had unusually high lead levels. The Turkish raisins (n=18) contained a mean (range) of 0.93 (0.056- 3.1)mg kg[sup -1] lead, whereas five samples from Australia, South Africa, Iran, Mexico and Chile contained a mean of 0.0085 (0.005-0.010)mg kg[sup -1]. Acid-washing studies showed that most of the lead in the Turkish raisins was on the surface of the fruit. The impact of eating the raisins on the dietary intake of lead was estimated for Canadians of different ages and sexes. For example, eating raisins from Turkey would increase the dietary intake of lead by 1-4-year-old children from 0.97 to ∼2.2 μg kg[sup -1] body weightday[sup -1]. The source of the lead was traced to use of a copper fungicide contaminated with high lead levels. Currently, lead levels in raisins imported from Turkey are low and approach levels in raisins from other countries. Uncontaminated raisins contain ∼0.01mg lead kg[sup -1], and a maximum tolerance for lead in raisins of 0.1mg kg[sup -1] is achievable irrespective of the type of raisin or country of origin. Therefore, consideration should be given to proposing this level as a maximum tolerance for lead in raisins. [ABSTRACT FROM AUTHOR]

Tur key ranks fourth in the world in vine yard acre age with 567,000 hect ares, and 3,700,000 met ric tons be ing pro duced an nually. Fresh ta ble grapes ac count for 25% of this pro duc tion while 17.5% is seedless rai sin grapes and 15% is seeded rai sin grapes. Of the re main ing produc tion 2.5% is used for wine and co gnac with the re main ing 40% be ing uti lized in juice, jelly, and other pro cessed prod ucts. Nearly all of the seed less rai sin grapes pro duced in the Aegean Region are dried nat u rally on the ground or on wire fences af ter dip ping into a potash (K2CO3) and ol ive oil so lu tion. Tur key, which is one of the lead ing seed less rai sin grape ex porting countries, has many prob lems with dry ing and mar ket ing. In this pa per, thedry ing meth ods used in Tur key and their effects on mar ket ing both in foreign and do mes tic mar kets along with op timal dry ing methods are discussed. [ABSTRACT FROM PUBLISHER]

A method is described for the determination of concentrations of the mycotoxin ochratoxin A in dried vine fruits (currants, raisins and sultanas) using acidic methanolic extraction,immunoaffinity chromatography clean-up and HPLC determination. The limit of detection was estimated as 0.2mug/kg, and recoveries of 63-77% were achieved at 5mug/kg. HPLC-mass spectrometric confirmation of the identity of ochratoxin was obtained. Ochratoxin A and aflatoxins were determined in 60 samples of retail dried vine fruits purchased in the United Kingdom. Ochratoxin A was found in excess of 0.2mug/kg in 19 of 20 currant, 17 of 20 sultana and 17 of 20 raisin samples examined, an overall incidence of 88% . The maximum level found was 53.6mug/kg. No aflatoxin was found in any sample analysed, using a method with a detection limit of 0.2mug/kg for each of aflatoxin B1, B2, G1 and G2. [ABSTRACT FROM AUTHOR]