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Epichloe endophytes (Clavicipitaceae) infect pooid grass genera worldwide but predominantly in the Northern Hemisphere, but appear to be rare in native grasses of the Southern Hemisphere. Because of benefits that hosts may receive from the symbiosis, Epichloe endophytes have been extensively studied and are considered important components of sustainable agriculture. There are only a few studies available on the incidence of endophyte infection in grasses of the Southern Hemisphere and most grass species have never been examined. Here we report on a survey of native grasses of New Zealand including 25 endemic or indigenous species. We sampled up to 10 plants per species at different sites from both the North and South Island of New Zealand and examined tissues microscopically for endophyte infection. Overall, only two species were found to be infected, Poa matthewsii (Matthew's bluegrass) and Dichelachne micrantha (short-hair plume grass). Based on analyses of tefA and tubB genes, both endophytes were found to be interspecific hybrids. The endophyte of the new host D. micrantha was previously described as Epichloe australiensis, while the endophyte of P. matthewsii is a new species named here E. novae-zelandiae. The new species is a hybrid derived from E. amarillans, E. bromicola and E. typhina subsp. poae. Alkaloid analyses in planta suggested that E. novae-zelandiae can produce small amounts of peramine, early pathway indole-diterpenes and ergot alkaloids, but no lolines or lolitrems. Target specific primers suggested the presence of genes for ergot alkaloids and peramine, but genes of only early pathway steps for the other alkaloids. Furthermore, genes for both mating type idiomorphs (MTA and MTB) were present, a single copy of MTA and two copies of MTB. Endophytes of native grasses may provide a genetic resource that could be exploited for developing pasture grass cultivars with improved performance. [ABSTRACT FROM AUTHOR]

Awe is a complex emotion composed of an appraisal of vastness and a need for accommodation. The purpose of this study was to develop a robust state measure of awe, the Awe Experience Scale (AWE-S), based on the extant experimental literature. In study 1, participants (N = 501) wrote about an intense moment of awe that they had experienced and then completed a survey about their experience. Exploratory factor analysis revealed a 6-factor structure, including: altered time perception (F1); self-diminishment (F2); connectedness (F3); perceived vastness (F4); physical sensations (F5); need for accommodation (F6). Internal consistency was strong for each factor (α ≥.80). Study 2 confirmed the 6-factor structure (N = 636) using fit indices (CFI =.905; RMSEA =.054). Each factor of the AWE-S is significantly correlated with the awe items of the modified Differential Emotions Scale (mDES) and Dispositional Positive Emotion Scale (D-PES). Triggers, valence, and themes associated with awe experiences are reported. [ABSTRACT FROM AUTHOR]

We compared Seligman’s PERMA model of well-being with Diener’s model of subjective well-being (SWB) to determine if the newer PERMA captured a type of well-being unique from the older SWB. Participants were 517 adults who completed self-report measures of SWB, PERMA, and VIA character strengths. Results from four analytic techniques suggest the factor underlying PERMA is capturing the same type of well-being as SWB. Confirmatory factor analysis yielded a latent correlation of r = 0.98 between SWB and PERMA. Exploratory structural equation modeling found two highly related factors (r = 0.85) that did not map onto PERMA and SWB. SWB and PERMA factors showed similar relationships with 24 character strengths (average correlation difference = 0.02). Latent profile analyses yielded subgroups of people who merely scored high, low, or mid-range on well-being indicators. Our findings suggest that while lower-order indicators SWB and PERMA have unique features, they converge onto a single well-being factor. [ABSTRACT FROM AUTHOR]

Lysergyl peptide synthetase 1 catalyzes the assembly of toxic ergopeptines from activated D-lysergic acid and three amino acids. The gene encoding this enzyme in the endophytic fungus Neotyphodium lolii was analyzed and compared to a homologous gene from the ergot fungus Claviceps purpurea . Each gene contained two introns, which were found in the same relative position within two modules of the gene. The 5′ ends of the two genes were unusually divergent. Signature sequences determining substrate specificity were similar in adenylation domains that recognized identical amino acids but differed within the adenylation domain for the amino acid that varies between the major ergopeptines of the two fungi. Homologues were detected in several related endophytic fungi; the tall fescue endophyte Neotyphodium coenophialum contained a divergent, second copy of the gene. Our results provide new information on the structure and distribution of this important peptide synthetase involved in ergot alkaloid biosynthesis. [ABSTRACT FROM AUTHOR]

Presents information on a study which examined the attitudes and beliefs held by middle school teachers and their students about teaching and learning geometry using Geometer's Sketchpad in a student-centered environment. Methodology; Results and discussion; Conclusions.

Investigates the effects of eighth grade students' working memory capacity, preference for amount of instruction, spatial problem-solving ability and school mathematics grades on achievement measures. Recall of factual information; Conceptual understanding of students.

The article argues the method in investigating the role of domain-general mechanisms to obtain domain-specific expertise is complete. It suggests that domains of expertise are different depending on the physical and mental abilities it relies. It also discusses that the ability to acquire expertise is constrained by general cognitive ability. It also discusses that the research has an incomplete understanding of the relationship of the domain general learning mechanisms to skill acquisition. It also suggests to investigate domains of expertise on a wider range to gain a complete understanding of the mechanisms affecting expert performance

\nHighlights Prior work suggests that differences in brain morphology and task-evoked neural activity may underlie extraordinary creative achievement. Here, we extend these findings by focusing on resting blood oxygen level–dependent (rsBOLD) functional connectivity differences between eminent creators from diverse fields of expertise and a “smart” comparison group of age- and education-matched non-eminent thinkers. Participants underwent rsBOLD imaging, along with a high-resolution structural brain scan, and completed a series of demographic and behavioral measures. The results revealed significant differences in rsBOLD connectivity between the eminent and noneminent thinkers. Eminent thinkers showed increased connectivity between (a) the right central operculum and the bilateral parietal operculum, right supramarginal gyrus, and left precentral gyrus; (b) the right precentral gyrus and the right postcentral gyrus; (c) the left parietal operculum and the right central operculum and the right sensorimotor cortex; and (d) the posterior cingulate gyrus and the right precentral gyrus. In contrast, they showed decreased connectivity between (a) the posterior cingulate gyrus and the left supracalcarine cortex and (b) between the left occipital fusiform gyrus and the left frontal operculum. These results suggest that extraordinary creativity may be associated with measurable differences in functional connectivity, particularly in areas that support creative functions. This study examined functional connectivity as measured by resting blood oxygen level–dependent MRI in individuals with exceptionally high creativity. Eminent creators from diverse fields were compared to an age- and education-matched control group. Eminence was assessed based on their Creative Achievement Questionnaire scores. Extraordinarily creative individuals showed increased functional connectivity between the left and right hemispheres and between frontoparietal and sensorimotor regions. These findings help elucidate the neurophysiological mechanisms of exceptionally high creativity. This study examined functional connectivity as measured by resting blood oxygen level–dependent MRI in individuals with exceptionally high creativity.Eminent creators from diverse fields were compared to an age- and education-matched control group.Eminence was assessed based on their Creative Achievement Questionnaire scores.Extraordinarily creative individuals showed increased functional connectivity between the left and right hemispheres and between frontoparietal and sensorimotor regions.These findings help elucidate the neurophysiological mechanisms of exceptionally high creativity. [ABSTRACT FROM AUTHOR]