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Matrix solid phase dispersion (MSPD) is a rapid and flexible sample pretreatment method that accomplishes sample disruption, extraction, filtering and purification in one step and is especially suitable for solid, semisolid and viscous samples. More than 30 years have elapsed since it was first proposed, and there are many published reviews. However, there is no review on the use of MSPD to extract active compounds from natural products. In this review, the latest developments from 2015–2021 in MSPD microextraction techniques, including sorbents, elution solvents and assisted MSPD extraction, along with their applications in natural products, are systematically discussed and classified according to the structure of the active constituents, which mainly refer to phenolic acids, flavonoids, alkaloids, anthraquinones and terpenes. [ABSTRACT FROM AUTHOR]

A variety of devices used in daily life and biomedical field will generate magnetic fields with different parameters, raising concern about their influences on people's physiological functions. Multiple experimental works have been devoted to the influences of magnetic fields on circadian rhythms, yet the findings were not always consistent due to the differences in magnetic field parameters and experimental organisms. Also, clear regulatory mechanisms have not been found. By systematizing the major achievements in research on magnetic and circadian rhythms based on magnetic flux density and analyzing the potential mechanisms of the magnetic fields affecting circadian rhythms, this review sheds light on the effects of magnetic fields on circadian rhythms and the potential applications in biomedicine. [ABSTRACT FROM AUTHOR]

Hallyu tourism has emerged as a theme for tourism research. Generally, existing studies just define Hallyu as the popular culture of South Korea (hereafter Korea) and then explain how Hallyu helps attract inbound tourists to Korea. There is no clear definition for Hallyu tourism. This research letter intends to close this gap by providing an illustration of Hallyu tourism as the amalgamation of film tourism, celebrity-induced tourism, food tourism and culture tourism. Existing studies related to the impacts of Hallyu on inbound tourists to Korea usually use questionnaire for data collection. To contribute to the literature, this paper empirically examines the impacts of Hallyu on inbound tourists to Korea with time-series data. The long-run relationship between Hallyu and inbound tourists to Korea is examined. This paper uses the exports of the content industry of Korea as the proxy for the expansion of Hallyu. To capture the mentioned long-run relationship, cointegration analysis taking into account of small sample size with only 15 annual observations is utilized. The findings conclude that Hallyu attracts inbound tourists and inbound tourists from Asia to Korea. [ABSTRACT FROM AUTHOR]

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Chemotherapy is currently the main treatment for unresectable or advanced postoperative gastric cancers. However, its efficacy is negatively affected by the occurrence of chemoresistance, which severely affects patient prognosis. Recently, dysregulation in autophagy has been suggested as a potential mechanism for chemoresistence, and long noncoding RNA (lncRNA) also shows its regulatory role in cancer drug resistance. Using RNA sequencing, we found that lncRNA EIF3J-DT was highly expressed in drug-resistant gastric cancer cells. In-vitro and in-vivo experiments showed that EIF3J-DT activated autophagy and induced drug resistance in gastric cancer cells by targeting ATG14. Bioinformatics and experimental results showed that EIF3J-DT regulated the expression of ATG14 through direct binding to enhance stabilization of ATG14 mRNA and via blocking the degradation of ATG14 mRNA through competitively binding with microRNA (miRNA) MIR188-3p. Therefore, EIF3J-DT increased the expression of ATG14, contributing to activation of autophagy and chemoresistance. Furthermore, it was confirmed that EIF3J-DT and ATG14 were highly expressed in gastric cancer patients resistant to chemotherapy, and this was closely associated with patient prognosis. In conclusion, EIF3J-DT is involved in the regulation of autophagy and chemoresistance in gastric cancer cells by targeting ATG14. It may be a suitable new target for enhancing chemosensitivity and improving prognosis. Abbreviations: 3-MA: 3-methyladenine; 5-Fu: 5-fluorouracil; ATG: autophagy related; C-CASP3: cleaved caspase 3; C-CASP7: cleaved caspase 7; C-PARP: cleaved PARP; CQ: chloroquine; CR: complete response; DIG: digoxigenin; ESR1: estrogen receptor 1; FBS: fetal bovine serum; FISH: fluorescence in situ hybridization; IHC: immunohistochemistry; ISH: in situ hybridization; lncRNA: long noncoding RNA; miRNA: microRNA; MUT: mutant; NC: negative control; OXA: oxaliplatin; PBS: phosphate-buffered saline; PD: progressive disease; PFA: paraformaldehyde; PR: partial response; qPCR: quantitative polymerase chain reaction; RAPA: rapamycin; SD: stable disease; TEM: transmission electron microscopy; WT: wild type. [ABSTRACT FROM AUTHOR]

Currently, studies have shown that anti-CGRP monoclonal antibodies are effective drugs for the prophylaxis and treatment of episodic migraine. Therefore, for the first time, we classified and concluded 10 treatment regimens according to the different doses, drugs, routes of administration, and courses of treatment, so as to provide a reference for further clinical studies. We studied relevant randomized controlled trials (RCTs) published before August 2020 on PubMed, Embase, Ovid MEDLINE, Web of Science, and the Cochrane Central Register of Controlled Trials. Eleven RCTs involving 6397 patients were included in our analysis. Network meta-analysis results suggested that in the comparison of the average migraine days per month, Erenumab (140 mg), Galcanezumab (120 mg, 240 mg), Fremanezumab (225 mg, 675 mg) were superior to placebo, Erenumab(7 mg), and the difference was statistically significant; Fremanezumab (225 mg, 675 mg) was superior to Erenumab (21 mg, 70 mg), and the difference was statistically significant; in the comparison of average medication days of acute migraine-specific drug per month, Erenumab (70 mg, 140 mg), Galcanezumab (120 mg, 240 mg), Fremanezumab (225 mg, 675 mg) was superior to placebo, and Erenumab (140 mg) and Galcanezumab (120 mg, 240 mg) were superior to Erenumab (70 mg), and the difference was statistically significant; there was no statistically significant difference in the average migraine days in the last month or in the medication days of acute migraine-specific drug. Fremanezumab (225 mg) and Galcanezumab (120 mg) may be the best clinical protocol after a comprehensive assessment. [ABSTRACT FROM AUTHOR]

Sodium glucose co-transporter 1 (SGLT1) triggers low glucose-induced glucagon-like peptide-1 (GLP-1) secretion. We reported that a two-week administration of 3-deoxyglucosone (3DG), an independent factor associated with the development of pre-diabetes, reduces basal GLP-1 secretion in rats. This study investigated the effects of 3DG on GLP-1 secretion and SGLT1 pathway under low-glucose conditions in STC-1 cells. STC-1 cells were incubated with phloridzin or 3DG at 5.6 mM glucose. SGLT1 expression (by western blotting), GLP-1 and cyclic adenosine monophosphate (cAMP) levels (by ELISA), and intracellular Ca2+ concentration (by Fluo-3/AM) were measured. Phloridzin inhibited GLP-1 secretion. SGLT1 protein expression in STC-1 cells cultured in 5.6 mM glucose is higher than that in 25 mM glucose. Exposure to 3DG for 6 h reduced GLP-1 secretion, SGLT1 protein expression, and intracellular concentrations of cAMP and Ca2+. 3DG reduces low glucose-induced GLP-1 secretion in part through reduction of SGLT1 expression. [ABSTRACT FROM AUTHOR]

While there has been a great deal of marketing research focusing on the impact of customer satisfaction on financial performance, limited attention is directed to examine the possible quadratic relationship between these variables. This paper aims to fill this gap. Utilizing the time series data of Marriott International from 1997 to 2016 and the appropriate time series econometric techniques that deal with small sample size, this study shows a U-shaped relationship linking customer satisfaction and firm's financial performance. This finding is important as it suggests the inappropriateness to assume implicitly a linear association between customer satisfaction and financial performance. A practical implication to the marketing practitioners is that satisfaction level needs to be enhanced beyond a threshold can the positive impact on financial performance be observed in the longer term. [ABSTRACT FROM AUTHOR]

Background: This study aimed to develop nomograms predicting the overall survival (OS) of patients younger than 50 years old with esophageal cancer. Methods: We selected patients included 2004–2015 in the Surveillance, Epidemiology, and End Results (SEER) database. Nomograms were constructed using significant variables from multivariable Cox analyses. The discrimination and calibration power of the models were evaluated using concordance indexes (C-indexes) and calibration curves. Decision curve analysis was used to assess the clinical net benefits of the nomograms. Results: Of 1,997 selected patients, 53.2% had advanced-stage tumor. Race, grade, T stage, N stage, and treatment were independent factors affecting OS in early-stage patients. The C-indexes of the corresponding nomogram were 0.710 (95% CI = 0.684–0.736) and 0.681 (95% CI = 0.640–0.722) in training and validation sets, respectively. Grade, marital status, and treatment were independent factors affecting OS in advanced-stage patients. The C-indexes of the corresponding nomogram were 0.677 (95% CI = 0.653–0.701) and 0.675 (95% CI = 0.638–0.712) in training and validation sets, respectively. Calibration curves demonstrated high consistency between predicted and actual survival. Conclusion: We constructed and verified nomograms that could accurately predict the survival rate of esophageal cancer in patients younger than 50 years old. This may help clinicians better understand prognostic factors. [ABSTRACT FROM AUTHOR]

PbZrxTi1-xO3 (PZT) films were fabricated on FTO substrates by using precursor solutions containing polyvinylpyrrolidone additive and spinning-coating/annealing process. The correlation of the formation, ferroelectric, dielectric and optical reflection features of the layered PZT films with Zr/Ti atomic ration had been studied. It was found that each PZT film was fully crystallized and displayed a polycrystalline phase. The PZT films with Zr content ∼ 0.4–0.6 showed clearly distinguishable layered structures, and exhibited excellent ferroelectric and dielectric properties, together with good optical performance as dielectric mirrors. These PZT films possessed high reflectivity bands, the band widths were all over 50 nm and the peak reflectivities were more than 70%. They also had dielectric constants >550 Fm−1 at 10 KHz, remnant polarizations >24 μC/cm2 at 100 V polarizing voltage, rendering their potential application in photonic band gap engineering and integrated optoelectronics. [ABSTRACT FROM AUTHOR]

In this paper, the consensus-based approach for the economic dispatch problem in microgrid via distributed event-triggered control is considered. Its basic principle is to utilise the consensus of the multi-agent systems consisting of generators and loads, which will work cooperatively such that the optimal incremental cost and optimal power output could be obtained to solve the economic dispatch problem. The consensus analysis of the proposed systems focused on the following two physical scenarios: (i) containing the power balance constraint but without generation limit; (ii) the power balance constraint and with generation limit. The corresponding effective distributed event-triggered controller is designed respectively to avoid the continuous communication between the agents, the state updating of each agent depending on its own state and its neighbouring agents at their last triggering time. Compared with the centralised event-triggered mechanism, the proposed distributed scheme could reduce the frequency of information exchange between the agent and its neighbours and save the bandwidth and energy. Simulation indicates the effectiveness of the theoretical results. [ABSTRACT FROM AUTHOR]

Fetal arrhythmias are common, and they may resolve spontaneously in majority of the cases. Sustained fetal arrhythmias associated with major structural heart disorders, hydrops fetalis, and fetal heart failure warrant intrauterine pharmaceutical conversion of heart rhythm or early pacemaker implant in order to avoid fetal demise. Fetal atrial flutter (AF) and supraventricular tachycardia (SVT) resemble in terms of the effects of intrauterine therapies. Digoxin is more suitable for rhythm conversion of fetal AF and SVT in fetuses free of hydrops fetalis, while sotalol shows better effects for those with hydrops fetalis. In fetal cases of atrioventricular blocks, an etiological treatment for the maternal antibody exposure by steroids could be an alternative remedy. In this article, the clinical diagnosis and treatment of fetal arrhythmias are presented, and advantages and disadvantages of antiarrhythmic agents for fetal arrhythmias are compared. [ABSTRACT FROM AUTHOR]

Background: Chlorogenic acid (CGA) has anti-oxidant and anti-inflammatory effects, but the study on its role in Alzheimer's disease (AD) models remains rare. Here, the effects of CGA on β-amyloid protein (Aβ)-induced cell models were investigated, aiming to provide a direction for Aβ-induced AD. Material and methods: Hippocampal neurons were separated from newborn Sprague-Dawley (SD) rats and identified by immumofluorescence method. Hippocampal neurons were processed with Aβ25-35 after pre-treatment CGA. MTT assay was used for detecting viability of treated cells. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and lactate dehydrogenase (LDH) of treated hippocampal neurons were determined by corresponding kits. Flow cytometry analysis assessed the apoptosis and mitochondrial membrane potential (MMP) in hippocampal neurons after treatment. The expressions of proteins related to apoptosis and endoplasmic reticulum stress (ERS) were measured by western blot (WB) analysis. Results: Immumofluorescence method showed that the Aβ25-35 induction models were successfully constructed. CGA increased the viability and decreased the apoptosis rate of Aβ25-35-induced hippocampal neurons. Decreasing activities of LDH and MDA, and raised contents of SOD and GSH-Px were appeared in Aβ25-35-induced cells that pre-treated with CGA. Moreover, CGA also enhanced MMP intensity of hippocampal neurons induced by Aβ25-35. In WB analysis, CGA reversed the promoting effect of Aβ25-35 on the expressions of proteins related to pro-ERS and pro-apoptosis. Conclusion: CGA restrained the apoptosis of Aβ25-35-induced hippocampal neurons via improving the anti-oxidant capacity, mitochondrial injury and ERS state of cells, which may provide a direction for AD. [ABSTRACT FROM AUTHOR]

Hb Bronovo [α103(G10)His→Leu, HBA2: c.311A>T] is an α-globin variant that interferes with and decreases binding efficiency to α hemoglobin (Hb) stabilizing protein (AHSP), a chaperone molecule. The histidine residue at position 103 is integral to the AHSP hydrogen bond formation where disruption results in an increased quantity of cytotoxic free α-globin chains, thereby creating a similar pathophysiology as β-thalassemia (β-thal). We report a family with Hb Bronovo, including a homozygous proband, which resulted from maternal uniparental disomy (UPD). Although not detected by routine studies in previous reports, the variant protein is visible by intact mass spectrometry (MS). [ABSTRACT FROM AUTHOR]

We conducted a cross-sectional survey of 1,540 researchers concerning their experiences with and attitudes toward the ethics of equal contribution (EC) designations in publications. Over half the respondents (58.3%) said they had been designated as an EC at least once. Although most respondents agreed that EC designations can be a useful way of promoting collaborations (81.7%) or resolving disagreements about authorship order (63.3%), a substantial proportion of respondents (38.1%) regarded these designations as useful but ethically questionable. 31.7% of respondents said EC designations are ethically questionable because ECs are difficult to define or measure and 25.9% said they are ethically questionable because people rarely contribute equally. Most respondents (71.8%) agreed that it is unfair to name two people as ECs when they have not contributed equally and that journals (73.4%), research teams (69.5%), and research institutions (63%) should develop policies concerning EC designations. Views concerning the ethics and policies of EC designations were influenced by the race/ethnicity and position of respondents but not by gender. Researchers who had been designated as ECs were less likely to regard this practice as ethically questionable than those who had not. [ABSTRACT FROM AUTHOR]

Patients with prolonged isolated thrombocytopenia (PT) after allogeneic stem cell transplantation (allo-SCT) are known to have a poor prognosis. However, there is no standard treatment for PT. The present study aimed to investigate the potential effect of a novel recombinant human thrombopoietin (rhTPO) in a cohort of patients with PT following allo-SCT. A total of 24 patients with PT (including delayed platelet engraftment and secondary failure of platelet recovery) were treated with rhTPO from July 1, 2016 to May 31, 2017. rhTPO injections were administered at 300 IU/kg/d for 28 consecutive days or until platelet counts were ≥ 50 × 109/L, independent of platelet transfusion. Response was defined as platelet recovery to ≥ 20 × 109/L for seven consecutive days without transfusion support during or within 7 days of the end of rhTPO treatment. All patients completed the 28 days of treatment, and none were withdrawn due to adverse effects. The overall response was 45.8%, which was significantly higher than historical data (12.2%, p < 0.001). The median response time was 12 (7–25) days from the initiation of rhTPO treatment. A response to rhTPO was associated with megakaryocytes in the bone marrow (positive vs. negative, 81.8 vs. 22.2%; p = 0.022). Among 11 patients exhibiting a response to rhTPO, the median number of megakaryocytes in bone marrow was increased significantly (10 vs. 2; p = 0.036) after rhTPO treatment. In conclusion, the results of this preliminary study suggest that rhTPO may represent a therapeutic option, with a response of 45.8% for patients with PT after allo-SCT, and especially for those with megakaryocytes in the bone marrow. However, this should be further confirmed in randomized prospective clinical trials. [ABSTRACT FROM AUTHOR]

We prepared octreotide (OCT)-modified curcumin plus docetaxel micelles to enhance active targeting and inhibit tumor metastasis by destroying vasculogenic mimicry (VM) channels. Soluplus was applied as an amphiphilic material to form micelles via film dispersion. The cytotoxic effects, active cellular targeting, and inhibitory effects on metastasis were systematically evaluated in vitro using A549 cells, and in vivo antitumor effects were evaluated using xenograft tumor-bearing mice. In vitro assays indicated that the OCT-modified curcumin plus docetaxel micelles showed robust cytotoxicity on A549 cells and effectively inhibited VM channels and tumor metastasis. Studying the mechanism of action indicated that OCT-modified curcumin plus docetaxel micelles downregulated MMP-2 and HIF-1α. In vivo assays indicated that OCT-modified curcumin plus docetaxel micelles increased drug accumulation at tumor sites and showed obvious antitumor efficacy. The developed OCT-modified curcumin plus docetaxel micelles may offer a promising treatment strategy for non-small-cell lung cancer. [ABSTRACT FROM AUTHOR]

Solanum betacea is a sub-tropical tree, and the fruit has high nutritional value, unique flavor and color. It is used as a functional ingredient in health care, food, cosmetic and pharmaceutical applications. The complete chloroplast(Cp) genome of S. betacea has been assembled and annotated in this paper. Its length was 155,937 bp, containing a large single-copy region of 86,731 bp, a small single-copy region of 18,450 bp, and a pair of IR regions of 25,378 bp in each. The complete chloroplast genome of S. betacea contained 134 genes, including 90 protein-coding genes, 36 transfer RNA genes (tRNAs), and 8 ribosome RNA genes (rRNAs). The overall GC content was 37.7% and the GC contents of the LSC, SSC, and IR regions were 35.7%, 31.8%, and 43.1%, respectively. Phylogenetic analysis with the reported chloroplast genomes revealed that S. betacea has been most closely related to Solanum torvum. These findings will provide useful information for further investigation of chloroplast genome evolution in Solanum betacea. [ABSTRACT FROM AUTHOR]

Prior research that examines the relationship between customer satisfaction and financial performance (FP) in the hotel sector assumes that changes in customer satisfaction lead to changes in the FP of hotels. This research note aims to bridge the gap by looking into the possibility that FP of hotels may lead to increase in customer satisfaction with the annual data of Marriott from 1995 to 2016. To study the existence of long-run interactions between customer satisfaction and FP with firm size that is proxied by number of employees as the control variable under small sample size condition, the presence of cointegration among these variables with different appropriate dependent variable is investigated with the bounds testing approach. The obtained results suggest that there is a positive long-run causality from FP to customer satisfaction, and customer satisfaction has no effect on FP in the long-run. This study also finds that firm size has no impact on customer satisfaction. Explanation on each of these findings is provided in the conclusion. The selected sample and availability of data limit the generalisability of the findings of this study. Different hotel brands, measurements and analysis techniques will further the understanding in this field. [ABSTRACT FROM AUTHOR]

Resistance to chemotherapy drugs, such as adriamycin (ADR), is a common problem in acute myeloid leukemia (AML) patients. We hypothesized that the natural compound resveratrol (Res) may reverse AML drug resistance through the PI3K/Akt/Nrf2 pathway. We investigated the in vitro effect of Res using human promyelocytic leukemia cells (HL-60) and the ADR-resistant cell line (HL-60/ADR) and treated with either Res or ADR + Res. Cellular proliferation inhibition rate, auto-fluorescence intensity of ADR in HL-60/ADR cells and HL-60 cells, mRNA expression of Nrf2 and the drug-resistant gene MRP1, and protein expression of PI3K, Akt, p-Akt, Nrf2, and MRP1 were measured. Results showed ADR + Res had a more significant inhibitory effect than ADR alone on HL-60/ADR cells. Auto-fluorescence intensity of ADR in HL-60/ADR cells treated with ADR + Res significantly increased. No difference of the auto-fluorescence intensity of ADR was observed in HL-60 cells treated with ADR and ADR + Res. mRNA expression of Nrf2 and MRP1 significantly decreased in HL-60/ADR cells treated with both Res and ADR + Res; protein expression of PI3K, p-Akt, Nrf2, and MRP1 significantly decreased in HL-60/ADR cells treated with PI3K inhibitor, Res and ADR + Res. In conclusion, Res reverses the drug resistance of AML HL-60/ADR cells through regulation of the PI3K/Akt/Nrf2 signaling pathway and MRP1 expression. [ABSTRACT FROM AUTHOR]

Salvia miltiorrhiza (Danshen in Chinese), is a well-known traditional Chinese medicinal plant, which is used as not only human medicine but also health-promotion food. Danshen has been extensively used for the treatment of various cardiovascular and cerebrovascular diseases. As a major group of bioactive constituents from S. miltiorrhiza, water-soluble phenolic acids such as salvianolic acid B possessed good bioactivities including antioxidant, anti-inflammatory, anti-cancer and other health-promoting activities. It is of significance to improve the production of phenolic acids by modern biotechnology approaches to meet the increasing market demand. Significant progresses have been made in understanding the biosynthetic pathway and regulation mechanism of phenolic acids in S.miltiorrhiza, which will facilitate the process of targeted metabolic engineering or synthetic biology. Furthermore, multiple biotechnology methods such as in vitro culture, elicitation, hairy roots, endophytic fungi and bioreactors have been also used to obtain pharmaceutically active phenolic acids from S. miltiorrhiza. In this review, recent advances in bioactivities, biosynthetic pathway and biotechnological production of phenolic acid ingredients were summarized and future prospective was also discussed. [ABSTRACT FROM AUTHOR]

In this paper, the leader-following consensus problem of the fractional-order nonlinear multi-agent systems via event-triggered control is considered. An effective event-triggered controller is designed and then generalised exponential consensus of the controlled multi-agent systems is studied in the sense of Mittag-Leffler stability of fractional-order systems. The event-triggering function design is dependent on the parameter of the system structure and the minimum inter-event interval can be flexibly adjusted with different fractional-order α. With the event-triggered control scheme, the consensus condition is obtained and the convergence rate of the system is estimated. Numerical simulation indicates the effectiveness of the theoretical results. [ABSTRACT FROM AUTHOR]

Data sharing is crucial to the advancement of science because it facilitates collaboration, transparency, reproducibility, criticism, and re-analysis. Publishers are well-positioned to promote sharing of research data by implementing data sharing policies. While there is an increasing trend toward requiring data sharing, not all journals mandate that data be shared at the time of publication. In this study, we extended previous work to analyze the data sharing policies of 447 journals across several scientific disciplines, including biology, clinical sciences, mathematics, physics, and social sciences. Our results showed that only a small percentage of journals require data sharing as a condition of publication, and that this varies across disciplines and Impact Factors. Both Impact Factors and discipline are associated with the presence of a data sharing policy. Our results suggest that journals with higher Impact Factors are more likely to have data sharing policies; use shared data in peer review; require deposit of specific data types into publicly available data banks; and refer to reproducibility as a rationale for sharing data. Biological science journals are more likely than social science and mathematics journals to require data sharing. [ABSTRACT FROM AUTHOR]

The consensus problem of a class of fractional-order linear multi-agent systems with distributed control containing input time-delay is investigated. By adopting the frequency domain analysis and generalised Nyquist criterion for the fractional-order time-delay systems, the delay-dependent consensus condition is obtained. Then, we are interested in finding the maximum time-delay tolerance and give an exact formula for calculating the maximum time-delay to maintain the system is consensusable. As illustrated by the numerical examples, the proposed theoretical results work effectively and accurately. [ABSTRACT FROM AUTHOR]

One of the most significant changes to the Common Rule is the requirement that institutions use a single Institutional Review Board (IRB) for cooperative research in the United States, unless more than one IRB is required by state, local, or tribal law, or a signatory federal agency decides an exception is warranted. We surveyed Human Research Protection Program (HRPP) officials at the top U.S. research institutions to understand their knowledge and opinion of the mandate, what steps their institutions are taking, and difficulties their institutions are facing. One-hundred seven institutions (56.9%) responded to the survey. While support for the single-IRB mandate was positive overall, most respondents acknowledged that their institution is likely to face some difficulties complying with it. Regulatory agencies can help institutions to comply with the mandate by providing guidance concerning such issues as exceptions to the mandate, local context review, oversight, and implementation of reliance agreements, and development of policies, procedures, and best practices. [ABSTRACT FROM AUTHOR]

Trichopria drosophilae (Hymenoptera: Diapriidae) is an important pupal endoparasitoid of Drosophila species, which has been found to be an ideal biocontrol agent to D. suzukii. In this study, the complete mitochondrial genome of T. drosophilae (GeneBank accession number: MN966974) was sequenced using Illumina HiSeq X Ten system. The mitochondrial genome is 16,375 bp long and comprises 13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. Among them, 24 genes are in majority strand, while the others are in minority strand. The nucleotide composition of A, G, C, T is 44.9%, 6.4%, 5.6%, 43.2% respectively. We also performed a phylogenetic analysis with other known mitochondrial genomes within four families that have been shown to parasitize drosophilid species. The result shows that T. drosophilae is closely related to Ismarus sp. [ABSTRACT FROM AUTHOR]

Asobara japonica is an important larval-pupal endoparasitoid of Drosophila melanogaster and some other fruit fly species, such as Drosophila suzukii, and is an invasive and economic pest. In this study, the complete mitochondrial genome of A. japonica (GeneBank accession number: MN882556) was sequenced using Illumina HiSeq X Ten system. The mitochondrial genome is 15,519 bp long and comprises 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, of which 12 genes are in majority stand, and the remaining 25 genes are in minority strand. The nucleotide composition of A, G, C, T is 40.6, 8.2, 6.0, and 45.3% respectively. We also performed a phylogenetic analysis with other known mitochondrial genomes of Braconidae. The results show that A. japonica is closely related to Diachasmimorpha longicaudata. [ABSTRACT FROM AUTHOR]

The t(11;14)(q13;32)-positive plasma cell neoplasms (PCNs) are common. In light of their lymphoplasmacytoid features and CD20 expression, we speculated that they may share laboratory features with B-cell lymphomas with plasmacytic differentiation (BCL-PCDs). We investigated flow cytometric CD19 and CD45 expression, DNA ploidy index and M-protein subtype in 416 t(11;14)-positive PCNs, as well as control groups (88 BCL-PCDs and 81 t(11;14)-negative PCNs). The plasma cells from the t(11;14)-positive PCNs were largely CD19−/CD45−, similar to the t(11;14)-negative PCNs and unlike the BCL-PCD plasma cells (p < .0001). 79% of the t(11;14)-positive PCNs were diploid, which was significantly more than in t(11;14)-negative PCNs (p < .0001) and significantly less than in the BCL-PCDs (p < .001). Although IgM secretion was common in BCL-PCDs (80%) and rare in PCNs (3%), most IgM PCNs (92%) were t(11;14)-positive. These findings may be helpful in evaluating specimens with clonal plasma cells in the reference laboratory setting. [ABSTRACT FROM AUTHOR]

The first example of autocatalytic hydrolyzation of 4-aryl-1-tosyl-1,2,3-triazoles induced by p-toluenesulfonic acid was reported, providing an effective and metal-free synthetic approach to deliver a broad range of new 4-aryl-2H-1,2,3-triazoles in good yields. The kinetic profile of this hydrolyzation suggested that this reaction has exponential autocatalytic behavior. [ABSTRACT FROM AUTHOR]

Prediction of uplift pile response is an interesting area of research in the field of geotechnical engineering. In the present approach, a hyperbolic model is adopted to simulate the relationship between pile displacement and unit skin friction developed at the pile–soil interface. Based on the hyperbolic model, a simple analytical approach is proposed to analyse the nonlinear response of a single pile subjected to tension load. Furthermore, comparisons of the results derived from the present method, other analytical methods and well-documented field experimental pile loading cases are made to demonstrate the effectiveness and accuracy of the proposed analytical methods. The proposed simple analytical approach is economical and efficient, and suitable for the analysis of a tension pile embedded into layered soils using a hyperbolic model. [ABSTRACT FROM AUTHOR]

In recent years, marketing researchers have studied the effect of customer satisfaction on firm performance, which is measured by either a firm's stock price or valuation (Aksoy, Cooil, Groening, Keiningham, & Yalcin, [1]). Such relationship exists because greater customer satisfaction improves customer loyalty and customer retention, which subsequently leads to improved financial performance, and thus, betters firm performance (Williams & Naumann, [5]). Second, the existence of elastic relationship from customer satisfaction to firm performance and the inelastic relationship from firm performance to customer satisfaction indicate that the effect of one variable on another is not the same. [Extracted from the article]

A letter to the editor is presented related to the article studies the normal natural Killer (NK)-cells as a characteristic of T-cell large granular lymphocytic leukemia.

Enhancer of zeste homolog 2 (EZH2), an epigenetic regulator and H3k27-specific histone methyltransferase, is important for transcriptional regulation. EZH2 has been found to be overexpressed in B-cell lymphomas, as well as some T-cell lymphomas. Here we investigated the expression of EZH2 by immunohistochemical staining in a wide range of T-cell neoplasms. We found that EZH2 is highly expressed in all categories of T-cell neoplasia studied, and its expression strongly correlates with a high proliferation rate. Although up-regulation of EZH2 has been reported to be modulated by the pSTAT3-MYC pathway, our data indicate that EZH2 expression is correlated with MYC and/or pSTAT3 expression in only a subset of T-cell lymphomas, and that other mechanisms may control the overexpression of EZH2 in many T-cell lymphomas. The high level of EZH2 expression in T cell lymphomas suggest that these neoplasms may benefit from targeted treatment with a small molecule inhibitor of EZH2 currently in use in clinical trials. [ABSTRACT FROM AUTHOR]

We propose a novel market-based approach to optimum inventory control in a doubly stochastic jump-diffusion economy by modelling a commodity distributor's inventory investment as a portfolio of forward commitments with explicit accounting of the jump-diffusion dynamics of demands, costs, and prices in open markets. We apply the robust real-asset martingale valuation methodology to derive a closed-form solution for the inventory value and a simple and intuitive optimality condition. Numerical analysis verifies this condition and demonstrates that the resulting optimum policy has robust properties in relation to the stylized effects. [ABSTRACT FROM AUTHOR]

Aim: To explore the relationship between plasma total homocysteine concentration and diabetic macular edema in patients with type 2 diabetes. Methods: Patients with type 2 diabetes ( n = 176) were enrolled in a cross-sectional hospital-based study. Diabetic macular edema status was documented by fundus photographs. Plasma total homocysteine concentration was measured using fluorescence polarization immunoassay. Normal control plasma homocysteine was established in 115 healthy subjects. Risk factors for diabetic macular edema were obtained from fasting blood samples and interviewer questionnaire. Results: Diabetic patients had increased plasma homocysteine compared with normal control. Plasma homocysteine levels were higher in subjects with diabetic macular edema than without ((11.4 ± 2.7) versus (8.5 ± 1.9) (μmol/l), p = 0.000). The association of homocysteine with diabetic macular edema was independent of major risk factors for diabetic macular edema (duration of diabetes, HbA1c, lipid) and determinants of higher homocysteine concentration (age, gender, serum folate and vitamin B12, renal status, and biguanide use) (OR: 1.63 (1.02-2.14), p = 0.018). Furthermore, per increase of 5.0 μmol/l plasma homocysteine was related to macular edema, after controlling for per unit increase of other factors (OR: 1.64 (1.04-2.16), p = 0.019). Conclusions: Plasma total homocysteine concentration is independently associated with the occurrence of macular edema in type 2 diabetes. Future prospective studies are warranted to clarify the relationship. [ABSTRACT FROM AUTHOR]

Transcription factor decoy oligodeoxynucleotides (ODN) represent a novel tool for targeted inhibition of the STAT3 signaling pathway. To investigate its therapeutic potential in ovarian cancer, a double-stranded decoy ODN mimicking STAT3-specific cis-elements was transfected into two ovarian cancer cell lines OVCAR3 and SKOV3. The STAT3 decoy ODN treatment specifically blocked STAT3 signaling, and inhibited cell proliferation by inducing apoptosis and cell cycle arrest. These results suggest that targeted blockade of the STAT3 signaling pathway with a decoy ODN may represent a potential therapeutic approach in the treatment of ovarian cancer. [ABSTRACT FROM AUTHOR]

Crateva unilocularis is naturally distributed in Southern China, which is an elite natural tree with high edible and medicinal value. In this study, whole chloroplast (cp) genome of Crateva unilocularis was assembled and characterized on the basis of Illumina pair-end sequencing data. The complete cp genome was 156,417 bp in length, containing a large single-copy region (LSC) of 85,607 bp and a small single-copy region (SSC) of 18,164 bp, which were separated by a pair of 26,323 bp inverted repeat regions (IRs). The genome contained 128 genes, including 85 protein-coding genes, 35 tRNA genes, and 8 rRNA genes. The overall GC content is 36.32%, while the corresponding values of the LSC, SSC, and IR regions were 33.98, 29.45, and 42.48%, respectively. The maximum-likelihood phylogenetic analysis showed a strong sister relationship with Crateva tapia. These findings provide a foundation for further investigation of cp genome evolution in Crateva unilocularis and other higher plants. [ABSTRACT FROM AUTHOR]

In this study, the mitochondrial genome (mitogenome) sequence of Pieris napi is determined using next-generation sequencing. The entire mitogenome genome is determined to be 15,178 bp in length. It contains 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and an adenine (A) + thymine (T)-rich region. The overall GC content of the genome is 19.9%. A phylogenetic tree reconstructed by 22 mitogenomes reveals that P. napi is most closely related to Pieris melete. [ABSTRACT FROM AUTHOR]

In this study, the mitochondrial genome (mitogenome) sequence of Papilio memno is determined using next-generation sequencing. The entire mitogenome genome is determined to be 15,262 bp in length. It contains 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and an adenine (A) + thymine (T)-rich region. The overall GC content of the genome is 20.3%. A phylogenetic tree reconstructed by 39 mitochondrial genomes reveals that P. domestica is most closely related to Prunus salicina. [ABSTRACT FROM AUTHOR]

Fraxinus malacophylla is one of the commonly used ecological restoration tree species in rocky desertification areas. It has high medicinal and timber value. And has high marketization prospects. The complete chloroplast genome sequence of F. malacophylla was generated by de novo assembly using whole-genome next generation sequencing. The complete chloroplast genome of F. malacophylla was 155621 bp in total sequence length and divided into four distinct regions: large single copy region (86404 bp), small single copy region (17821 bp), and a pair of inverted repeat regions (25698 bp). The F. malacophylla chloroplast genome annotation predicted a total of 131 genes, consisting of 35 tRNA genes, 8 rRNA genes, and 88 protein-coding genes. Phylogenetic analysis with the reported chloroplast genomes revealed that F. malacophylla has most closely related to F. excelsior. [ABSTRACT FROM AUTHOR]

Irradiation of the recipient site has been associated with postoperative complications and an increased rate of flap failure in facial reconstruction. The aim of this study was to report the outcomes of our use of four different types of flaps (two free and two pedicled) for the reconstruction of patients with nasopharyngeal carcinoma (NPC) treated with radiotherapy, who subsequently received salvage surgery after the development of recurrence or secondary primary tumours. The records of 12 NPC patients who underwent salvage surgery and reconstruction from 2002-2007 were retrospectively reviewed. There were no intraoperative or significant postoperative complications. All flaps survived. The average cosmetic outcome was 2.7 (1 = poor, 4 = excellent), all patients could tolerate a liquid to normal diet, and nine patients had normal or intelligible speech, while three exhibited slurred speech. These results indicate that the effects of irradiation on the recipient site do not appear to adversely affect successful flap transfer or outcomes. [ABSTRACT FROM AUTHOR]

Objectives: The aim of this article is to make an evaluation on the clinical features of patients with Kawasaki disease who require a cardiac surgical procedure including coronary artery bypass grafting, coronary arterial aneurysmorrhaphy or heart transplantation. Methods: English literature of Kawasaki disease for cardiac surgery (1990-2011) was retrieved in the Pubmed database. The clinical features of the patient setting from the representative articles were collected and analyzed. Results: Patients with Kawasaki disease were very young, with some requiring a cardiac surgical procedure at a very early age. The interval between the onset and the surgical operation was 9.5 ± 9.4 years. The prevalence of myocardial infarction and re-infarction was high. Giant aneurysm, critical stenosis with calcification and thrombus formation of the coronary arteries often warrant coronary artery bypass, heart transplantation or coronary arterial aneurysm plication. The left internal mammary artery to the left anterior descending coronary artery was the most commonly used graft in coronary artery bypass. Graft patency rate was 82.4% at 21.4 ± 32.3 (range 0.1-252) month follow-up. The early and late mortalities of this patient setting were 0.6 and 3.0%, respectively. Conclusions: Patients with Kawasaki disease may develop coronary artery lesions prone to aneurysmal formation with calcification and thrombus and may require coronary artery bypass at a very early age. With the left internal mammary artery as the first choice of bypass graft, the long-term patency and patient survival was satisfactory. [ABSTRACT FROM AUTHOR]

Conclusion: The radial forearm free flap (RFFF) is one of the optimal choices for hypopharyngeal reconstruction. Our series demonstrates that the technique of an indicator flap for the monitoring of the buried flap is simple, reliable, and inexpensive. The condition of the indicator flap can be easily interpreted by the physicians and the nursing staff. Therefore, the success rate of this microsurgical reconstruction may be improved. Objectives: The RFFF is increasingly applied in reconstruction of the hypopharynx after radical resection for advanced hypopharyngeal cancer. However, postoperative monitoring of the buried free flap is extremely difficult. We designed a small external component as an indicator flap to monitor the perfusion of the buried vascular pedicle. Methods: Eight consecutive patients with hypopharyngeal cancer underwent radical surgery and hypopharyngeal reconstruction using RFFF at the Sun Yat-sen University Cancer Center between January 2005 and January 2007. The indicator flap was sutured to the surface of the neck for postoperative monitoring. Results: All of the indicator flaps remained viable. One patient experienced vascular compromise and was successfully salvaged. The success rate of the buried flaps was 100%. Pharyngocutaneous fistula occurred in one patient. All patients resumed an oral diet eventually. [ABSTRACT FROM AUTHOR]