44 results on '"Takahashi, Jun"'
Search Results
2. Perinatal outcome in case of maternal death for cerebrovascular acute disorders: a nationwide study in Japan.
- Author
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Katsuragi, Shinji, Tanaka, Hiroaki, Hasegawa, Junichi, Kurasaki, Akiko, Nakamura, Masamitsu, Kanayama, Naohiro, Nakata, Masahiko, Hayata, Eijiro, Murakoshi, Takeshi, Yoshimatsu, Jun, Osato, Kazuhiro, Tanaka, Kayo, Oda, Tomoaki, Sekizawa, Akihiko, Shimaoka, Takako, Ishiwata, Isamu, Takahashi, Jun, Ikeda, Tomoaki, and Maternal Death Exploratory Committee in Japan and Japan Association of Obstetricians and Gynecologists
- Subjects
CEREBROVASCULAR disease ,PERINATAL death ,QUESTIONNAIRES ,ASPHYXIA neonatorum ,MATERNAL mortality ,ASPHYXIA ,DISEASE complications - Abstract
Objective: The goal of this study is to find clues to improve perinatal outcomes in the case of cerebrovascular acute disorders during pregnancy.Study Design: We analyzed 35 cases of cerebrovascular diseases related to maternal deaths in Japan those that occurred during pregnancy and reported to the Committee of the Ministry of Health, Labor, and Welfare from 2010 to 2018.Results: Cerebrovascular acute disorders occurred at 34.6 ± 6.6 gestational weeks. There were seven intrauterine fetal deaths (IUFD), and eight cases showed neonatal asphyxia with umbilical arterial pH between 6.7 and 7.0 (asphyxia cases, n = 15). In two of eight newborns, brain hypothermia therapy was given, and all survived without neurological sequelae. Maternal dyspnea was significantly related to severe prolonged decelerations of the fetus (p < .05), and asphyxia cases (p < .005). Median time from maternal onset to delivery (OD time) was significantly longer in asphyxia cases than in the non-asphyxia cases (84 vs 29 min, p < .05). OD time over 30 min was significantly related to the antepartum occurrence, cervical dilatation <5 cm (p < .05), onset outside of the hospital (p < .001), and maternal transfer before delivery (p < .001).Conclusion: More than 40% of cases experienced fetal asphyxia, and 20% ended in IUFD in maternal deaths related to cerebrovascular acute disorders. Maternal respiratory support and rapid delivery would be the keys to improve perinatal outcomes in case of cerebrovascular acute disorders during pregnancy. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
3. Stroke during pregnancy and puerperium among Japanese women: a single-center registry.
- Author
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Katsuragi, Shinji, Suzuki, Rieko, Toyoda, Kazunori, Neki, Reiko, Miyamoto, Susumu, Iihara, Koji, Takahashi, Jun, Ikeda, Tomoaki, and Yoshimatsu, Jun
- Subjects
STROKE ,JAPANESE women ,HEMORRHAGIC stroke ,ISCHEMIC stroke ,MISCARRIAGE ,PUERPERIUM - Abstract
Stroke is one of the major causes of maternal death. This study aimed to analyze the maternal and fetal outcomes of stroke occurred during pregnancy and puerperium. We conducted a retrospective analysis of patients admitted to our perinatology center between 1982 and 2012 with a diagnosis of acute cerebral stroke during pregnancy or within 6 weeks postpartum. Thirty-four patients were registered and all the patients had never been diagnosed as stroke nor detected cerebrovascular abnormalities before the current pregnancies. They were divided into 8 ischemic strokes (ISs) and 26 intracranial hemorrhage group. In the hemorrhage group, there was a spontaneous abortion and two patients chose artificial abortions to avoid rehemorrhage, and there were another three intrauterine fetal deaths (IUFDs) in the acute stage of maternal stroke. More patients in hemorrhage group delivered in preterm than in IS group for the treatment of stroke, 10/23 (43%) versus 0/8 (0%), p <.05. More patients in hemorrhage group had low Glasgow Coma Scale (GCS) (3–8) than in IS group at the onset of the stroke, 12/26 (46%) versus 0/8 (0%), p <.05. There were three maternal deaths and 6/23 (26%) were neurologically dependent in hemorrhage group in the chronic stage, whereas 87% were independent in IS group, p <.05. Hemorrhagic stroke was more common etiology of stroke related to pregnancy than IS in this study. Intensive and multidisciplinary care was needed especially in hemorrhagic stroke related to pregnancy as in the hemorrhagic stroke the fetal survival rate was lower, and maternal conscious levels at the onset of the stroke and neurological outcomes in the chronic stage were worse than IS. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
4. STOCHASTICITY MODELING AND ANALYSIS OF ELASTIC MODULUS OF RANDOMLY ORIENTED STRANDS.
- Author
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Wan, Yi, Zhang, Xin, Sato, Yu, and Takahashi, Jun
- Subjects
ELASTIC analysis (Engineering) ,ELASTIC modulus ,ELASTICITY ,STATISTICS ,MULTISCALE modeling - Abstract
Randomly oriented strands (ROS) exhibit multiscale inhomogeneous and significant stochastic variations even in elastic properties because of their complex internal geometries. To quantify the variations and expand the applicability of ROS in industrial fields, a laminate analogy analytical model was developed in combination with Monte Carlo simulation-based statistical analysis to compute the mean and variance of the elastic modulus of ROS. Two semi-empirical fitting equations were formulated to calculate the mean and variance of the elastic modulus, thus reducing the need for onerous experiments. The effects of strand morphology on the stochasticity of the elastic properties are discussed in detail for the first time. The results of the analytical model showed significant applicability and good agreement with the experimental data under various conditions, and the formulated equations were able to provide satisfactory values for the mean and variance of the elastic modulus with different strand properties and strand/specimen morphologies. The role of the strand morphology (thickness, width, and length) was analyzed from a statistical perspective and the thickness ratio of the strand to the specimen was found to be the most effective factor for reducing the stochasticity of ROS. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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- View/download PDF
5. Complete mitochondrial DNA sequence of the Japanese endemic catfish Silurus lithophilus (Siluriformes: Siluridae).
- Author
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Kishimoto, Yuu, Okuyama, Hisashi, and Takahashi, Jun-ichi
- Subjects
NUCLEOTIDE sequence ,DNA sequencing ,MITOCHONDRIAL DNA ,TRANSFER RNA ,STOP codons ,CATFISHES - Abstract
The Japanese endemic catfish Silurus lithophilus is distributed only in Lake Biwa, Lake Yogo, and their river drainages. There are four species of the genus Silurus in Japan, of which S. lithophilus has a limited distribution. This catfish needs to be collected for DNA data owing to the lack of information related to its conservation. Here, the complete mitochondrial genome of the S. lithophilus from Lake Biwa in Japan was analyzed using next-generation sequencing. The mitochondrial genome of S. lithophilus was identified as a 16,524 bp circular molecule containing 13 protein-coding genes (PCGs), 22 tRNA genes, and 2 rRNA genes, along with one A + T-rich control region. The AT content was 55.89%. The heavy (H)-strand was predicted to have 12 PCGs and 15 tRNA, and 2 rRNA genes, whereas the light (L)-strand was predicted to contain one PCG and seven tRNA genes. The start codons ATG and GTG were found in 13 PCGs. The stop codons TAA, TAG, and AGA were observed in all PCGs, except CytB. All tRNA genes formed typical cloverleaf secondary structures. The molecular phylogenetic relationship, inferred using 13 PCGs (based on the maximum likelihood), was consistent with that reported in previous studies, which predicted a sister relationship between S. lithophilus and S. asotus. The results also clearly indicated that S. lithophilus is a valid species. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
6. The study of microstructure characterization: Cu modified Cu-Ni-graphite composite.
- Author
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Wang, Yiran, Gao, Yimin, Takahashi, Jun, Wan, Yi, He, Xiangdong, Zhang, Yunqian, Xiao, Bing, and Zhang, Chao
- Subjects
COMPOSITE coating ,POWDER metallurgy ,MICROSTRUCTURE ,GRAPHITE ,INTERFACIAL bonding ,PARTICULATE matter - Abstract
Cu-coated graphite was applied to modify the interface of Cu-Ni-graphite composite used as switch slide baseplate. Up to now, interfacial characterization and modification mechanisms of Cu modified Cu-Ni-graphite composite are seldom researched. In this study, Cu-coated graphite was deposited by chemical plating, and Cu modified Cu-Ni-graphite composite was prepared by powder metallurgy. Both the microstructure characterizations of Cu-coated graphite and interfacial morphologies of the composite were investigated. Then, the comparison of mechanical properties of unmodified and Cu modified Cu-Ni-graphite composite was discussed. The results showed that the Cu coating was made of fine particles with uniform distribution and with the average thickness of 1.05 μm. The mutual diffusion was about 2.54 μm width at the interface. The interfacial modification mechanism of Cu coating in the composite was that the decreasing of diffusion activity both in the graphite and matrix; and the increase of diffusion driving force at the interface. The interfacial bonding method in the transition from mechanical bonding to the combination of mechanical bonding and diffusion bonding. Compared with the unmodified composite, the flexural strength raised 24.4%, and hardness increased by 9%. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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- View/download PDF
7. Stroke during pregnancy and puerperium among Japanese women: a single-center registry.
- Author
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Katsuragi, Shinji, Suzuki, Rieko, Toyoda, Kazunori, Neki, Reiko, Miyamoto, Susumu, Iihara, Koji, Takahashi, Jun, Ikeda, Tomoaki, and Yoshimatsu, Jun
- Abstract
Background: Stroke is one of the major causes of maternal death. This study aimed to analyze the maternal and fetal outcomes of stroke occurred during pregnancy and puerperium.Methods: We conducted a retrospective analysis of patients admitted to our perinatology center between 1982 and 2012 with a diagnosis of acute cerebral stroke during pregnancy or within 6 weeks postpartum.Results: Thirty-four patients were registered and all the patients had never been diagnosed as stroke nor detected cerebrovascular abnormalities before the current pregnancies. They were divided into 8 ischemic strokes (ISs) and 26 intracranial hemorrhage group. In the hemorrhage group, there was a spontaneous abortion and two patients chose artificial abortions to avoid rehemorrhage, and there were another three intrauterine fetal deaths (IUFDs) in the acute stage of maternal stroke. More patients in hemorrhage group delivered in preterm than in IS group for the treatment of stroke, 10/23 (43%) versus 0/8 (0%), p < .05. More patients in hemorrhage group had low Glasgow Coma Scale (GCS) (3-8) than in IS group at the onset of the stroke, 12/26 (46%) versus 0/8 (0%), p < .05. There were three maternal deaths and 6/23 (26%) were neurologically dependent in hemorrhage group in the chronic stage, whereas 87% were independent in IS group, p < .05.Conclusions: Hemorrhagic stroke was more common etiology of stroke related to pregnancy than IS in this study. Intensive and multidisciplinary care was needed especially in hemorrhagic stroke related to pregnancy as in the hemorrhagic stroke the fetal survival rate was lower, and maternal conscious levels at the onset of the stroke and neurological outcomes in the chronic stage were worse than IS. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
8. L-shaped structures made from ultrathin chopped carbon fiber tape reinforced thermoplastics: delamination behavior and optimization.
- Author
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Guo, Qitao, Ohsawa, Isamu, and Takahashi, Jun
- Subjects
CARBON fibers ,THERMOPLASTICS ,TENSILE strength ,STRUCTURAL optimization ,ADHESIVE tape ,BEND testing ,DELAMINATION of composite materials - Abstract
We examined L-shaped structures prepared from ultrathin chopped carbon fiber tape reinforced thermoplastics (UT-CTT), attempting to mitigate their delamination and minimize interlaminar out-of-plane tensile stress using four-point bending tests and finite element modeling. The reasonable agreement between experimental and simulated results was achieved, and interlaminar tensile strength was obtained by finite element modeling and analytical methods. Additionally, free-shape optimization was utilized. The structural optimization problem was formulated using interlaminar tensile stress as the objective function (with constraints placed on structural stiffness and weight), and thickness variations of the inner parts of the L-shaped structure were used as design variables. Optimization reduced the interlaminar tensile stress of the L-shaped structure from 53.77 to 37.29 MPa while maintaining its original stiffness and weight, suggesting that the described approach is generally applicable to determining the interlaminar tensile strength of UT-CTT and can be used to increase the delamination initiation load. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
9. Comparative analysis of mitochondrial genomes of the honey bee subspecies A. m. caucasica and A. m. carpathica and refinement of their evolutionary lineages.
- Author
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Ilyasov, Rustem, Nikolenko, Alexei, Tuktarov, Varis, Goto, Kenji, Takahashi, Jun-Ichi, and Kwon, Hyung Wook
- Published
- 2019
- Full Text
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10. Low mitochondrial DNA variation in the endangered bumble bee Bombus cryptarum florilegus.
- Author
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Takeuchi, Tsuyoshi, Sasaki, Takuma, Mitsuhata, Masahiro, Kiyoshi, Takuya, Nishimoto, Mana, Nomura, Tetsuro, and Takahashi, Jun-ichi
- Published
- 2019
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- View/download PDF
11. Influence of small amount of glass fibers on mechanical properties of discontinuous recycled carbon fiber-reinforced thermoplastics.
- Author
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Wei, Haowen, Nagatsuka, Wataru, Ohsawa, Isamu, Sumimoto, Ken, and Takahashi, Jun
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GLASS fibers ,CARBON fibers ,THERMOPLASTICS ,FLEXURAL modulus ,FLEXURAL strength ,CAUSATION (Philosophy) - Abstract
The effect of residual contamination, such as glass fibers (GFs), on the application of discontinuous recycled carbon fibers (rCFs) is still unclear. In this study, GFs are used to substitute rCF at volume fractions (Vfs) of 0%, 1%, 2%, and 5% in carbon fiber paper-reinforced thermoplastics fabricated through a papermaking process. After an investigation with the three-point bending tests, the flexural modulus will not decrease when the GF content increased. Unlike the flexural modulus, the flexural strength decreased with an increase in the GF volume. In order to illustrate the effect of small amount of GF on flexural modulus, a modified rule of mixture (ROM) is used to derive a coefficient, Factor c
1 , depicting the effect caused by discontinuous and randomly distributed reinforcement fibers. Furthermore, modified Halpin–Tsai model with Factor c1 illustrated that the experiment results of flexural modulus is not decreased as fast as predicted results. Therefore, small amount of GF will not affect the application of rCF. [ABSTRACT FROM AUTHOR]- Published
- 2019
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12. Complete mitochondrial genome sequence of the broad-winged damselfly, Mnais pruinosa Selys, 1853 (Odonata: Calopterygidae).
- Author
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Okuyama, Hisashi, Kiyoshi, Takuya, Takahashi, Jun-Ichi, and Tsubaki, Yoshitaka
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NUCLEOTIDE sequencing ,TRANSFER RNA ,STOP codons ,ODONATA ,NUCLEOTIDES ,GENOMES - Abstract
In this study, we analyzed the complete mitochondrial genome of the Mnais pruinosa Selys, 1853 from Saga Prefecture, Japan. The mitochondrial genome of M. pruinosa was identified as a circular molecule of 15,494 bp, and was found to be similar to that of other damselfly species. It was predicted to contain 13 protein-coding (PCG), 22 tRNA, and two rRNA genes, as well as one A + T-rich control region. The genes ATP8 and ATP6 shared seven nucleotides, ATP6 and COIII shared one nucleotide, ND4 and ND4L shared seven nucleotides, and ND6 and Cytb shared one nucleotide. The initiation codon ATG was found in eight genes, ATC in four, and ATT in one; the termination codons TAA, TAG, incomplete TA, and single T were observed in seven, one, two, and three genes, respectively. All the tRNA genes possessed a cloverleaf secondary structure, except for tRNA-His that lacks the TΨC loop. The average AT content of mitochondrial genome was 66.18%. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
13. Analysis of the complete mitochondrial genomes of two dwarf honeybee species, <italic>Apis florea</italic> and <italic>Apis andreniformis</italic> (Insecta: Hymenoptera: Apidae), in Thailand.
- Author
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Takahashi, Jun-ichi, Deowanish, Sureerat, and Okuyama, Hisashi
- Subjects
HONEYBEE genetics ,INSECTS ,MITOCHONDRIAL DNA ,NUCLEOTIDE sequence ,TRANSFER RNA - Abstract
The dwarf honeybees
Apis florea andApis andreniformis inhabit the bush and forests of continental Asia and north Africa and some islands of Sundaland and the Philippines. We analysed, for the first time, the complete mitochondrial genomes of two dwarf honeybee species from Thailand using next-generation sequencing. Each mitochondrial genome was a circular and approximately 17 kbp molecule that included 13 protein-coding genes (PCGs), 22 tRNA genes, and two ribosomal RNA genes, along with one A + T-rich control region, besides three tRNA-Ser (AGN ) repeats. The AT content values of the mitochondrial genomes ofA. florea andA. andreniformis were 86.28% and 85.73%, respectively. The 1150 mutation sites in 13 PCGs differing betweenA. florea andA. andreniformis in Thailand were evenly distributed throughout their mitochondrial genomes. The phylogenetic relationship, inferred using 13 PCGs, was consistent with that reported in previous studies, which predicted a sister relationship betweenA. florea andA. andreniformis . [ABSTRACT FROM AUTHOR]- Published
- 2018
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- View/download PDF
14. Mechanical properties of carbon fiber paper reinforced thermoplastics using mixed discontinuous recycled carbon fibers.
- Author
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Wei, Haowen, Nagatsuka, Wataru, Lee, Hooseok, Ohsawa, Isamu, Sumimoto, Ken, Wan, Yi, and Takahashi, Jun
- Subjects
CARBON fiber-reinforced plastics ,FIBER-reinforced plastics ,COMPRESSION molding ,FLEXURAL strength ,FLEXURAL modulus - Abstract
Market demand for carbon fiber (CF) increases annually. Therefore, owing to the growing amount of carbon fiber-reinforced plastic (CFRP) waste, CFRP manufacturers are attempting to develop and design materials and products by paying greater attention to ecological and economic factors. In this report, recycled CF (rCF) varieties with differing mechanical properties are mixed to determine the possibility of rCF application in future structural materials. 1, 3, 5, 8 and 10 MPa are investigated for proper compression molding pressure. The range of 5–8 MPa is recommended. Mixing different rCFs can control the mechanical properties of the material, which is valuable for current rCF applications. A modified rule of mixture (ROM) is used to discuss flexural modulus and flexural strength. The coefficients for ROM are given for different rCF. [ABSTRACT FROM PUBLISHER]
- Published
- 2018
- Full Text
- View/download PDF
15. Impact of pregnancy on the size of small cerebral aneurysm.
- Author
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Tanaka, Hiroaki, Katsuragi, Shinji, Tanaka, Kayo, Iwanaga, Naoko, Yoshimatsu, Jun, Takahashi, Jun C., and Ikeda, Tomoaki
- Subjects
INTRACRANIAL aneurysms ,PREGNANCY ,MAGNETIC resonance angiography ,SUBARACHNOID hemorrhage ,PUERPERIUM ,ANEURYSMS ,CARDIOVASCULAR diseases in pregnancy ,EVALUATION of medical care ,RETROSPECTIVE studies ,DISEASE complications - Abstract
Objective: Because the management of cerebral aneurysms during pregnancy remains controversial, the objective of this study was to perform a retrospective review of pregnant women with cerebral aneurysms to determine whether pregnancy is a risk factor for aneurysm rupture.Methods: The change in size of aneurysms during pregnancy was examined by performing repeat magnetic resonance angiography in five pregnant women with cerebral aneurysms between 2005 and 2013. The outcome examined was development of subarachnoid hemorrhage.Results: Among the five pregnancies, there were eight cerebral aneurysms. Subarachnoid hemorrhage did not developed during any pregnancy. In four of the pregnancies, the cerebral aneurysms did not change in size during pregnancy, remaining 2-5 mm. Although in one pregnancy, the aneurysm increased from 6 to 7 mm during the third trimester, it returned to its original size in the postpartum period.Conclusions: We suggest that if the cerebral aneurysm is small (≤ 5 mm), it is likely to remain unchanged despite the increase in circulating blood volume during pregnancy. Cerebral aneurysms greater than 5 mm but without blebs, irregular shape, high-risk location, or increased aspect ratio are also at low risk of rupture and are not likely to change during pregnancy. [ABSTRACT FROM AUTHOR]- Published
- 2017
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16. Preparing for first human trial of induced pluripotent stem cell-derived cells for Parkinson's disease: an interview with Jun Takahashi.
- Author
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Takahashi, Jun
- Abstract
Professor Jun Takahashi received his MD from Kyoto University in 1986 and his PhD in neurosurgery and neurobiology from Kyoto University Graduate School of Medicine in 1993 (both Kyoto, Japan). He then did a postdoctoral fellowship at the Salk Institute (Dr Fred Gage's Laboratory, CA, USA) where he started research work on neural stem cells. Since returning to Kyoto University Hospital in 1997, he has been investigating functional neurosurgery including deep brain stimulation and cell-based therapies for Parkinson's disease. He has been at the Center for iPS Cell Research and Application at Kyoto University since 2010 and is currently a professor in the Department of Clinical Application. His research interest is the development of cell replacement therapy using induced pluripotent stem cells for intractable neurological diseases and is currently leading the first clinical trial to investigate induced pluripotent stem cell-derived cells for PD, which started recruiting on 1 August 2018. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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- View/download PDF
17. Drug discovery toward successful cell transplantation therapy for Parkinson's disease using human pluripotent stem cells.
- Author
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Nishimura, Kaneyasu and Takahashi, Jun
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- 2016
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18. Complete mitochondrial DNA sequence of the Japanese endemic catfish Silurus biwaensis (Siluriformes: Siluridae) from Lake Biwa.
- Author
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Kishimoto, Yuu, Okuyama, Hisashi, and Takahashi, Jun-ichi
- Subjects
NUCLEOTIDE sequence ,DNA sequencing ,MITOCHONDRIAL DNA ,TRANSFER RNA ,STOP codons ,CATFISHES - Abstract
The Japanese endemic catfish Silurus biwaensis is distributed only in Lake Biwa and Yodo river drainages. There are four species of the genus Silurus in Japan, of which S. biwaensis has a most limited distribution. This catfish needs to be collected for DNA data owing to the lack of information related to its phylogenetic relationship. Here, the complete mitochondrial genome of the S. biwaensis from Lake Biwa in Japan was analyzed using next-generation sequencing. The mitochondrial genome of S. biwaensis was identified as a 16,531 bp circular molecule containing 13 protein-coding genes (PCGs), 22 tRNA genes, and 2 rRNA genes, along with one A + T-rich control region. The AT content was 55.83%. The heavy (H)-strand was predicted to have 12 PCGs, 14 tRNA, and 2 rRNA genes, whereas the light (L)-strand was predicted to contain one PCG and eight tRNA genes. The start codons ATG, ATC, and GTG were found in 13 PCGs. The stop codons TAA, TAG, and AGA were observed in all PCGs, except CytB and COX3. All tRNA genes formed typical cloverleaf secondary structures. The molecular phylogenetic relationships estimated using 13 PCGs (maximum-likelihood method) indicated that S. biwaensis is genetically distinct from the sympatric species S. asotus and S. lithophilus. This result clearly indicated that S. biwaensis is a valid species. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
19. The complete mitochondrial genome of the bumblebee, <italic>Bombus hypocrita sapporensis</italic> (Insecta: Hymenoptera: Apidae) from Hokkaido Island, Japan.
- Author
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Nishimoto, Mana, Umezawa, Misuzu, Okuyama, Hisashi, Kumano, Norikuni, Nomura, Tetsuro, and Takahashi, Jun-ichi
- Subjects
BUMBLEBEES ,INSECTS ,MITOCHONDRIAL DNA ,RIBOSOMAL RNA ,TRANSFER RNA - Abstract
The complete is mitochondrial genome of the Japanese bumblebee
Bombus hypocrita sapporensis from Hokkaido Island, Japan is analysed using next generation sequencing. The mitochondrial genome ofB. h. sapporensis was observed to be a circular molecule of 15,835 bp. The average AT content in theB. h. sapporensis mitochondrial genome was 85.53%. It was predicted to contain 13 protein-coding genes (PCGs), 22 tRNA genes, and two rRNA genes, along with one A + T-rich control region. The PCGs had ATA, ATG, or ATT as the initiation codon and were terminated by the typical stop codon TAA, except forCytb . All the tRNA genes typically formed a cloverleaf secondary structure, except fortrnE ,trnF , andtrnS1 . The molecular phylogenetic analysis indicated that theB. h. sapporensis from Hokkaido Island population was most similar to that of the geographically isolatedB. h. sapporensis from Rebun Island. [ABSTRACT FROM AUTHOR]- Published
- 2018
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- View/download PDF
20. Complete mitochondrial DNA sequence of the alien hornet <italic>Vespa velutina</italic> (Insecta: Hymenoptera) invading Kyushu Island, Japan.
- Author
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Takahashi, Ryoichi, Okuyama, Hisashi, Minoshima, Yûsuke N., and Takahashi, Jun-Ichi
- Subjects
VESPA (Genus) ,INSECTS ,MITOCHONDRIAL DNA ,NUCLEOTIDE sequence ,RIBOSOMAL RNA - Abstract
We analyzed the complete mitochondrial genome of the invasive Asian hornet
Vespa velutina from Kyushu Island, Japan. The mitochondrial genome ofV. velutina was identified as a circular molecule of 16,388 bp. We predicted that the genome contains 13 protein-coding genes (PCGs), 22 tRNA genes, and 2 rRNA genes, along with one A + T-rich control region. The average AT content is 81.68%. Molecular phylogenetic analysis using the 13 mitochondrial PCGs from 11 closely related taxa of Vespidae indicated that theV. velutina invading the Japanese Islands of Kyushu and Tsushima have a common origin. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
21. Investigation about the fracture behavior and strength in a curved section of CF/PP composite by a thin-curved beam specimen.
- Author
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Matsuo, Tsuyoshi, Goto, Takeshi, and Takahashi, Jun
- Subjects
DIAGNOSIS of bone fractures ,COMPOSITE materials ,TENSILE strength ,COMPRESSIVE strength ,DEFORMATIONS (Mechanics) - Abstract
A curved beam specimen with a curved section bent at a right angle and straight arms is an efficient experimental method to evaluate the interlaminar tensile strength of a composite laminate. This paper describes a technique to apply such a test method to determining the fracture behavior and strength in a curved section of a thin laminate. The proposed technique is capable of estimating appropriately the compressive or tensile strength as well as the interlaminar tensile strength at failure in a thin-curved section, especially targeting thermoplastic composite materials. The significant point of the proposed technique is a modified calculating method of the stress distribution in a curved section by taking into account the large deformation of the specimen because of thin laminate. [ABSTRACT FROM PUBLISHER]
- Published
- 2015
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- View/download PDF
22. Quantitative analysis by surface plasmon resonance of CD28 interaction with cytoplasmic adaptor molecules Grb2, Gads and p85 PI3K.
- Author
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Higo, Kunitake, Oda, Masayuki, Morii, Hisayuki, Takahashi, Jun, Harada, Yohsuke, Ogawa, Shuhei, and Abe, Ryo
- Subjects
SURFACE plasmon resonance ,CYTOPLASM ,T cells ,BIOSENSORS ,N-terminal residues ,C-terminal residues - Abstract
CD28 surface receptors provide co-stimulatory signals that are required for full T cell activation. The CD28 cytoplasmic region has one YMNM and two PXXP motifs as a functional motif. Upon CD28 ligation, Grb2, Gads, and the p85 subunit of PI3 kinase are recruited to the CD28 cytoplasmic region. Here, the interactions between these adaptor proteins and CD28 cytoplasmic domains were analyzed using a Biacore surface plasmon resonance biosensor. For all three adaptor proteins, entire molecules bound more tightly to CD28 than did their isolated SH2 domains. For each adaptor, different outcomes of mutation of CD28's PXXP motifs on binding affinity indicated that only the SH3 domain of Grb2 bound directly. Regarding binding of SH2s to CD28, the SH2 domains of p85 bound more strongly than those of both Grb2 and Gads. Since intact p85 had a 50-fold higher binding affinity than its fragments, and yet the p85-CD28 interaction does not involve SH3-PXXP binding, binding of both N-terminal and C-terminal SH2s to YMNM may create an 'avidity' effect. In contrast, when Grb2 and Gads interact with CD28, binding of their SH3 domains may be important. These results suggest that all these interactions are multivalent, through both SH2 and SH3 domains. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
23. Chaos computing: a unified view.
- Author
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Munakata, Toshinori, Takahashi, Jun, Sekikawa, Munehisa, and Aihara, Kazuyuki
- Subjects
- *
DISTRIBUTED computing , *COMPUTER systems , *COMPUTER networks , *LOCAL area networks , *CLIENT/SERVER computing - Abstract
Chaos computing is a non-traditional new paradigm that exploits the extreme non-linearity of chaotic systems. This article presents a unified theoretical view of chaos computing. It introduces the fundamental concept and the unique features that are characteristics of chaos computing, and discusses various implementation approaches. Basic aspects of digital chaos computing to realise logical gates are introduced, followed by two specific techniques: (1) direct utilisation of the threshold mechanisms; (2) an application of the chaos neuron model. After presenting these approaches, we discuss general characteristics of digital chaos computing. Other digital, analog and digital/analog hybrid forms of chaos computing are also considered. Potential advantages of chaos computing include: high speed, low power and low cost, a general-purpose form of computing, re-configurable or dynamic logical architecture, implementation of continuous logic, robustness against noise, and parallel and distributed computing. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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24. Stem cell therapy for Parkinson's disease.
- Author
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Takahashi, Jun
- Subjects
PARKINSON'S disease ,BRAIN diseases ,CELLULAR therapy ,TRANSPLANTATION of organs, tissues, etc. ,EMBRYONIC stem cells ,IMMUNOREGULATION ,CYTOKINES - Abstract
The aim of stem cell therapy for Parkinson's disease is to reconstruct nigro-striatal neuronal pathways using endogenous neural stem/precursor cells or grafted dopaminergic neurons. As an alternative, transplantation of stem cell-derived dopaminergic neurons into the striatum has been attempted, with the aim of stimulating local synapse formation and/or release of dopamine and cytokines from grafted cells. Candidate stem cells include neural stem/precursor cells, embryonic stem cells and other stem/precursor cells. Among these, embryonic stem cells are pluripotent cells that proliferate extensively, making them a good potential donor source for transplantation. However, tumor formation and ethical issues present major problems for embryonic stem cell therapy. This review describes the current status of stem cell therapy for Parkinson's disease, as well as future research approaches from a clinical perspective. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
25. Complete mitochondrial DNA sequence of the parasitic honey bee mite Varroa destructor (Mesostigmata: Varroidae).
- Author
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Harada, Reona, Yoshioka, Masato, Okuyama, Hisashi, Kato, Manabu, Martin, Stephen J., and Takahashi, Jun-ichi
- Subjects
VARROA destructor ,PERCIFORMES ,HONEYBEES ,NUCLEOTIDE sequence ,MITOCHONDRIAL DNA ,PARASITIFORMES ,TRANSFER RNA - Abstract
Varroa destructor is a parasite mite of the eastern honey bee Apis cerana, which is native to Asia. The European honey bee Apis mellifera was imported to Asia from Europe and the USA for apiculture in the 19th century. In a short period of time, V. destructor parasitized the artificially introduced honey bees. Varroa destructor was estimated to have spread around the world with A. mellifera when it was exported from Asia to locations worldwide about 50 years ago. The mitochondrial DNA of the parasitic honey bee mite V. destructor was analyzed using next-generation sequencing. The complete mitochondrial genome of V. destructor was identified as a 16,476-bp circular molecule containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and one AT-rich control region. The heavy strand was predicted to have nine PCGs and 13 tRNA genes, whereas the light strand was predicted to contain four PCGs, nine tRNA genes, and two rRNA genes. All PCGs began with ATA as the start codon, except COIII and CytB, which had ATG as the start codon. Stop codons were of two types: TAA for eight genes and TAG for five genes. Molecular phylogenetic analysis revealed that V. destructor from Japan was genetically distant from that of France. A high base substitution rate of 2.82% was also confirmed between the complete mitochondrial DNA sequences of V. destructor from Japan and the USA, suggesting that one Varroa mite strain found in the USA is not from Japan. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
26. A comparison of complete mitochondrial DNA sequences of Mnais costalis Selys, 1869 (Odonata: Calopterygidae) from three different populations (one allopatric and two sympatric).
- Author
-
Okuyama, Hisashi, Kiyoshi, Takuya, Takahashi, Jun-Ichi, and Tsubaki, Yoshitaka
- Subjects
NUCLEOTIDE sequence ,MITOCHONDRIAL DNA ,ODONATA ,TRANSFER RNA ,STOP codons ,BODY size - Abstract
In Japan, two closely-related damselflies, Mnais costalis Selys, 1869 (Odonata: Calopterygidae) and M. pruinosa Selys-Longchamps (Odonata: Calopterygidae), 1853, coexist, and they exhibit geographic variations in wing color, body size, and habitat preference. In this study, we analyzed the complete mitochondrial genome of M. costalis from Saga Prefecture, Japan (sympatric populations that exhibit wing color polymorphism), and compared the genome with M. costalis that exhibit monomorphic orange wing color. The mitochondrial genome of M. costalis from Saga Prefecture was identified as a circular molecule of 15,488 bp, similar to that found in other M. costalis populations. It was predicted to contain 13 protein-coding (PCG), 22 tRNA, and two rRNA genes, along with one A + T-rich control region. Among the PCGs, ATP8 and ATP6, ATP6 and COIII, ND4 and ND4L, and ND6 and Cytb shared seven, one, seven, and one nucleotides, respectively. The initiation codon ATG was found in eight genes, ATC in four, and ATT in one, while the termination codons TAA, TAG, TA, and T were observed in seven, one, two, and three genes, respectively. All the tRNA genes possessed a cloverleaf secondary structure, except for tRNA-His that lacks the TΨC loop. The average AT content of mitochondrial genome was 66.06%. From a phylogenetic analysis, the loss of wing color polymorphism in monomorphic sympatric populations is likely to occur with the coexistence of two Mnais species. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
27. The influence of thermal shock on inter-laminar shearing strength of woven C/C composites.
- Author
-
Fukuda, Hiroshi, Itohiya, Go, Watanabe, Jun, Takahashi, Jun, and Kemmochi, Kiyoshi
- Subjects
FIBROUS composites ,CARBON fibers ,SHEAR (Mechanics) ,MECHANICAL shock - Abstract
Although the mechanical properties of carbon fiber reinforced carbon (C/C) composites under high temperature environments have been widely studied to date, the influence of thermal shocks on C/C composites have scarcely been investigated due to the lack of high-speed heating equipment. Our equipment based on a high frequency induction heating method provided a solution to this problem. In the present study, we examined the influence of thermal shocks on the inter-laminar shearing strength (ILSS), which is one of the most important subjects of designing a plain woven C/C composite with a three point bending test. By the change of the span-to-height ratio (l/h), the failure mode shift of the inter-laminar shearing to the bending was observed. By a series of tests, we proposed an appropriate l/h ratio to obtain a reliable inter-laminar shearing strength of C/C composites, which would be higher than that for the conventional CFRPs. The observation of the failure specimens was done by scanning electron microscopy (SEM) and optical microscopy. Not only debondings at 0°/90° interface but also propagation of transverse cracks were observed in the thermal shocked specimens. The 0°/90° interfacial debondings were suggested as the main source of the inter-laminar shearing strength deterioration. [ABSTRACT FROM AUTHOR]
- Published
- 2000
- Full Text
- View/download PDF
28. Complete mitochondrial DNA sequence of the small hive beetle Aethina tumida (Insecta: Coleoptera) from Hawaii.
- Author
-
Takahashi, Jun-ichi, Okuyama, Hisashi, and Martin, Stephen J.
- Subjects
TRANSFER RNA ,EPINEPHELUS ,PERCIFORMES ,NUCLEOTIDE sequence ,MITOCHONDRIAL DNA ,INSECTS ,BEETLES ,GENETIC distance - Abstract
We analyzed the complete mitochondrial genome of the small hive beetle Aethina tumida, a major honeybee pest that was accidentally transported to the Hawaii Islands, USA. The mitochondrial genome of A. tumida was identified as a 15,198 bp circular molecule containing 13 protein-coding genes, 22 tRNA genes, and two rRNA genes, along with one A + T-rich control region. The average AT content was found to be 76.2%. The heavy strand was predicted to have nine protein-coding and 14 tRNA genes; while the light strand was predicted to contain four protein-coding, eight tRNA, and two rRNA genes. Among the 13 PCGs, the initiation codon ATT was found in five, ATA in three, ATG in four, and TTG in one gene. Six PCGs used TAA as the stop codon, whereas three PCGs used TAG as the stop codon. Incomplete stop codon TA (COIII, ND5, and ND4) and T (COII) were identified. We found that the genetic distance between A. tumida from Hawaii and the beetles' natural home South Africa, was small, indicating that the Hawaii population may have originated for the South Africa region. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
29. Complete mitochondrial DNA sequence of the three-keeled pond turtle Mauremys reevesii (Reptilia: Testudines).
- Author
-
Asami, Mari, Okuyama, Hisashi, and Takahashi, Jun-ichi
- Subjects
TRANSFER RNA ,EMYDIDAE ,MITOCHONDRIAL DNA ,NUCLEOTIDE sequence ,TURTLES ,REPTILES - Abstract
The complete mitochondrial genome of the three-keeled pond turtle Mauremys reevesii from Japan was analyzed using next-generation sequencing. The mitochondrial genome of M. reevesii was observed to be a circular molecule of 15,528 bp containing 13 protein-coding genes, 22 tRNA genes, and two rRNA genes, along with one A + T-rich control region. The average AT was found to be 61.11%. One extra base was present at the 174th position in the ND3 gene. Molecular phylogenetic analysis revealed that M. reevesii from Japan was few genetically distant from the geographically isolated M. reevesii from South Korea. This turtle might have been exported to Japan from the Korean Peninsula after the Middle Ages. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
30. Complete mitochondrial DNA sequence of the endemic Japanese pond turtle Mauremys japonica (Reptilia: Testudines).
- Author
-
Asami, Mari, Okuyama, Hisashi, and Takahashi, Jun-ichi
- Subjects
TRANSFER RNA ,RIBOSOMAL RNA ,EMYDIDAE ,MITOCHONDRIAL DNA ,NUCLEOTIDE sequence ,TURTLES ,REPTILES - Abstract
The complete mitochondrial genome of the Japanese pond turtle Mauremys japonica from Japan was analyzed using next-generation sequencing. The mitochondrial genome of M. japonica was identified as a 16,443 bp circular molecule containing 13 protein-coding genes (PCGs), 22 tRNA genes, and 2 rRNA genes, along with 1 A + T-rich control region; the average AT content was 62.82%. One extra base was present at the 174th position in the ND3 gene. The phylogenetic analyses of the complete mitochondrial DNA genes strongly supported the result obtained from the phylogenetic analysis of partial DNA sequences, grouping the monophyletic species within the genus Mauremys. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
31. The complete mitochondrial genome of the dusky brown-gray-colored honeybee, Apis mellifera (insecta: Hymenoptera: Apidae) of New Zealand.
- Author
-
Maeda, Mito, Nakagawa, Ikumi, Chikano, Mao, Okuyama, Hisashi, Murray, Robert, and Takahashi, Jun-Ichi
- Subjects
MITOCHONDRIAL DNA ,HONEYBEES ,HYMENOPTERA ,RIBOSOMAL RNA ,TRANSFER RNA - Abstract
We analyzed the complete mitochondrial genome of the dusky brown-gray-colored honeybee Apis mellifera, collected from North Island, New Zealand. We determined that the mitochondrial genome was a 16,336 bp and predicted 13 protein-coding genes (PCGs), 22 tRNA genes, and two rRNA genes. The start codon ATA was found in two genes, ATG in four genes, ATT in six genes, and ATC in one gene, whereas the termination codon TAA was observed in all PCGs. The non-coding regions of tRNA-Leu and COII were consistent with the C haplotype of A. mellifera carnica. Phylogenetic analysis suggests a close relationship with the European A. mellifera. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
32. The complete mitochondrial genome of a Buckfast bee, <italic>Apis mellifera</italic> (Insecta: Hymenoptera: Apidae) in Northern Ireland.
- Author
-
Okuyama, Hisashi, Hill, John, Martin, Stephen John, and Takahashi, Jun-ichi
- Subjects
HONEYBEE genetics ,INSECTS ,MITOCHONDRIAL DNA ,RIBOSOMAL RNA ,TRANSFER RNA - Abstract
We analyzed the complete mitochondrial genome of the ‘Buckfast bee’,
Apis mellifera, collected from North Ireland, UK. It consisted of a circular molecule of 16,353 bp. The genome contained 13 protein-coding, 22 tRNA, and 2 rRNA genes, along with one A + T-rich control region. The average AT content was 84.9%. The genes ATP8 and ATP6 shared 19 nucleotides. A phylogenetic analysis, suggested that the matriline ‘Buckfast bee’ has remained most closely related to theA. mellifera ligustica race from which it originated in 1917, despite being cross-bred with many otherA. mellifera races over the past 100 years. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
33. The complete mitochondrial genome of the yellow coloured honeybee <italic>Apis mellifera</italic> (Insecta: Hymenoptera: Apidae) of New Zealand.
- Author
-
Nakagawa, Ikumi, Maeda, Mito, Chikano, Mao, Okuyama, Hisashi, Murray, Robert, and Takahashi, Jun-Ichi
- Subjects
HONEYBEES ,INSECTS ,MITOCHONDRIAL DNA ,INSECT genomes ,TRANSFER RNA - Abstract
The complete mitochondrial genome of the yellow coloured honeybee
Apis mellifera from North Island, New Zealand was analyzed using next-generation sequencing. The mitochondrial genome was a 16,349bp circular molecule and was predicted to contain 13 protein-coding genes (PCGs), 22 tRNA genes and two rRNA genes. The initiation codon ATA was found in two genes, ATG in four genes, ATT in six genes, and ATC in one gene, while the termination codon TAA was observed in all the PCGs. Phylogenetic analysis using the sequence of 23 closely related taxa suggested a sister relationship with the Italian strainA. mellifera ligustica . [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
34. Complete mitochondrial DNA sequence of the tropical hornet Vespa affinis (Insecta, Hymenoptera).
- Author
-
Okuyama, Hisashi, Martin, Stephen J., and Takahashi, Jun-Ichi
- Subjects
MITOCHONDRIA ,NUCLEOTIDE sequencing ,HYMENOPTERA ,TRANSFER RNA - Abstract
We analyzed the complete mitochondrial genome of the Asian tropical hornetVespa affinisfrom Ishigaki Island, Japan. It consisted of a circular molecule with 19,109 bp, which is larger to other hornet species e.g.V. velutina. We predicted the genome contained 13 protein-coding, 22 tRNA, and two rRNA genes, along with one A + T-rich control region. The repetitive sequences were confirmed at multiple positions in the non-coding genes. The initiation codons ATA was found in one, ATG in seven, and ATT in five genes, while the termination codons TAA and TAG were observed 11 and two genes, respectively. The average AT content of the genome was 78.4%. [ABSTRACT FROM PUBLISHER]
- Published
- 2017
- Full Text
- View/download PDF
35. The complete mitochondrial DNA sequence of endemic honeybee Apis nuluensis (Insecta: Hymenoptera: Apidae) inhabiting Mount Kinabalu in Sabah Province, Borneo Island.
- Author
-
Takahashi, Jun-Ichi, Tingek, Salim, and Okuyama, Hisashi
- Subjects
APIS (Insects) ,INSECTS ,MITOCHONDRIAL DNA ,NUCLEOTIDE sequencing ,HONEYBEE genetics ,INSECT genomes - Abstract
The cavity-nesting honeybeeApis nuluensisinhabits only the highlands of Mount Kinabalu of Sabah, Borneo Island. The mitochondrial genome is a circular molecule of approximately 1.6 kb that includes 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one AT-rich control region. The average AT content was 84.5%. The start codons ATC, ATG, and ATT were found in one, three, and nine genes, respectively, whereas the stop codon TAA was observed in all genes. The phylogenetic relationship, inferred using 13 PCGs, was consistent with that reported in previous studies that predicted a sister taxon relationship betweenA. nuluensisandA. cerana. [ABSTRACT FROM PUBLISHER]
- Published
- 2017
- Full Text
- View/download PDF
36. The complete mitochondrial genome of the cavity-nesting honeybee, Apis cerana (Insecta: Hymenoptera: Apidae) from Borneo.
- Author
-
Okuyama, Hisashi, Tingek, Salim, and Takahashi, Jun-ichi
- Subjects
MITOCHONDRIAL DNA ,APIS cerana ,NUCLEOTIDE sequencing ,HYMENOPTERA ,GENETIC distance ,TRANSFER RNA - Abstract
The complete mitochondrial genome of the cavity-nesting honeybeeApis ceranafrom Sabah on Borneo Island was analyzed using next-generation sequencing. The mitochondrial genome ofA. ceranawas a circular molecule of 15,884 bp and was similar to that of the other cavity-nesting honeybee species. The average AT content in theA. ceranamitochondrial genome was 84.4%. It was predicted to contain 13 protein-coding, 22 tRNA, and two rRNA genes, along with one A + T-rich control region. [ABSTRACT FROM PUBLISHER]
- Published
- 2017
- Full Text
- View/download PDF
37. A stochastic modelling framework for predicting flexural properties of ultra-thin randomly oriented strands.
- Author
-
Xu, Xiaodong, Jesus, André, Wan, Yi, and Takahashi, Jun
- Abstract
A stochastic modelling framework is developed to predict the flexural properties of high-strength sheet moulding compounds made of randomly oriented ultra-thin carbon fibre-reinforced thermoplastic prepreg tapes. The model enables reliable designs using ultra-thin randomly oriented strands with less testing, leading to potential applications in automotive primary structures. The stochastic model is based on a Monte Carlo simulation. The flexural modulus is predicted using classical laminate theory, while the flexural strength is predicted by following a Weibull distribution. Fibre discontinuities are considered through stress concentrations introduced by tape overlaps. The results are validated against new 3-point bending and previously reported 4-point bending experimental results. A scaling effect on strength and scatter is predicted, and its implications for structural applications are also discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
38. Complete mitochondrial DNA sequence of the invasive hornet Vespa velutina (Insecta, Hymenoptera) found in Japan.
- Author
-
Takahashi, Ryoichi, Okuyama, Hisashi, Kiyoshi, Takuya, and Takahashi, Jun-ichi
- Subjects
INSECTS ,MITOCHONDRIAL DNA ,VESPA (Genus) ,INTRODUCED insects ,TRANSFER RNA - Abstract
In this study, we analyzed the complete mitochondrial genome of the invasive Asian hornet Vespa velutina from Japan. The mitochondrial genome of V. velutina was identified as a circular molecule of 16,765 bp, similar to that in other hornet species. It was predicted to contain 13 protein-coding, 20 tRNA, and two rRNA genes, along with one A + T-rich control region. The initiation codons ATC was found in one, ATG in four, ATT in five, and ATA in three genes, while TAA was the termination codon in all these genes. The average AT content of 13 protein-coding was 82%. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
39. Complete mitochondrial genome of the Japanese bumblebee, Bombus hypocrita hypocrita (Insecta: Hymenoptera: Apidae).
- Author
-
Nishimoto, Mana, Okuyama, Hisashi, Kiyoshi, Takuya, Nomura, Tetsuro, and Takahashi, Jun-ichi
- Subjects
INSECTS ,MITOCHONDRIAL DNA ,BUMBLEBEES ,APIDAE ,MOLECULAR structure ,RECOMBINANT DNA - Abstract
In the present report, we describe the complete mitochondrial genome of the common bumblebee, Bombus hypocrita hypocrita, from the Otome Plateau, in Yamanashi Prefecture, Japan. The mitochondrial genome of B. h. hypocrita is a circular molecule of 15,795 bp. It contains 13 protein-coding, 22 tRNA and two rDNA genes. The protein-coding genes had ATA, ATG or ATT as the initiation codon and were terminated by the typical stop codon TAA, except for ND4 and Cytb. All the tRNA genes typically formed a cloverleaf secondary structure, except for trnE and trnS1. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
40. The complete mitochondrial genome of the cavity-nesting honeybee, Apis koschevnikovi (Insecta: Hymenoptera: Apidae).
- Author
-
Wakamiya, Takeshi, Tingek, Salim, Okuyama, Hisashi, Kiyoshi, Takuya, and Takahashi, Jun-ichi
- Subjects
INSECTS ,MITOCHONDRIAL DNA ,HONEYBEES ,HYMENOPTERA ,NEST building ,REPEATED sequence (Genetics) - Abstract
In this study, we analyzed the complete mitochondrial genome of the cavity-nesting honeybee, A. koschevnikovi. The mitochondrial genome of A. koschevnikovi was observed to be a circular molecule of 15,278 bp and was similar to that of the other cavity-nesting honeybee species. The average AT content in the A. koschevnikovi mitochondrial genome was 84%. It was predicted to contain 13 protein-coding, 24 tRNA and two rRNA genes, along with one A + T-rich control region, besides three tRNA-Met repeats. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
41. Complete mitochondrial genome of the Japanese bumblebee, Bombus hypocrita sapporensis (Insecta: Hymenoptera: Apidae).
- Author
-
Takahashi, Jun-ichi, Nishimoto, Mana, Wakamiya, Takeshi, Takahashi, Moe, Kiyoshi, Takuya, Tsuchida, Koji, and Nomura, Tetsuro
- Subjects
BUMBLEBEES ,INSECTS ,MITOCHONDRIAL DNA ,INSECT phylogeny ,TRANSFER RNA ,CLASSIFICATION of insects - Abstract
In the present study, we describe the complete mitochondrial genome of the bumblebee,Bombus hypocrita sapporensisfrom the Rebun Island, in Hokkaido, Japan. The mitochondrial genome ofB. hypocrita sapporensisincludes a circular molecule of 15 700 bp. It contains 13 protein-coding genes, 22 tRNA genes, two rDNA genes and an A + T-rich control region. All protein-coding genes are initiated by ATA, ATG, and ATT codons and are terminated by the typical stop codon TAA or T, except forND4L, which ends with TA. All tRNA genes typically form a cloverleaf secondary structure. [ABSTRACT FROM PUBLISHER]
- Published
- 2016
- Full Text
- View/download PDF
42. The complete mitochondrial genome of the Japanese honeybee, Apis cerana japonica (Insecta: Hymenoptera: Apidae).
- Author
-
Takahashi, Jun-ichi, Wakamiya, Takeshi, Kiyoshi, Takuya, Uchiyama, Hironobu, Yajima, Shunsuke, Kimura, Kiyoshi, and Nomura, Tetsuro
- Subjects
HONEYBEE genetics ,MITOCHONDRIAL DNA ,APIDAE ,INSECT phylogeny ,TRANSFER RNA ,STOP codons ,MOLECULAR structure of RNA ,INSECTS - Abstract
In this study, we analyzed the complete mitochondrial genome of the Japanese honeybeeApis cerana japonica. The mitochondrial genome ofA. c. japonicais a circular molecule of 15 917 bp and is similar to that ofA. c. cerana. It contains 13 protein-coding genes, 22 tRNA genes, two rRNA genes and one A + T-rich control region. All protein-coding genes are initiated by ATT and ATG codons and are terminated by the typical stop codon TAA or TAG, except for the start codon ofATP8which ends with C. All tRNA genes typically form a cloverleaf secondary structure, except fortRNA-Ser(AGN). [ABSTRACT FROM PUBLISHER]
- Published
- 2016
- Full Text
- View/download PDF
43. Risk of Tumorigenesis and Patient Hope.
- Author
-
Fujita, Misao, Hatta, Taichi, Sawai, Tsutomu, and Takahashi, Jun
- Subjects
PARKINSON'S disease treatment ,TUMOR risk factors ,HEMATOPOIETIC stem cell transplantation ,HOPE ,PATIENT education - Abstract
The article focuses on the risk of tumorigenesis for patients with transplanted cells. Discussed is the strategy for therapeutic misconception in an informed consent process. Explored is the clinical use of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs) of patients with Parkinson's disease (PD).
- Published
- 2015
- Full Text
- View/download PDF
44. A case of adult-onset Grisel’s syndrome.
- Author
-
Nakai, Ami, Uehara, Masashi, Miyaoka, Yoshinari, Oba, Hiroki, Ikegami, Shota, Takizawa, Takashi, Munakata, Ryo, Hatakenaka, Terue, Kamanaka, Takayuki, Kurogochi, Daisuke, Fukuzawa, Takuma, and Takahashi, Jun
- Abstract
Abstract
Aim: Grisel’s syndrome is a non-traumatic subluxation of the atlanto-axial joint that occurs after infection or inflammation in the otolaryngological area, primarily in children.Method: This report describes the clinical characteristics of an extremely rare case of adult-onset Grisel’s syndrome.Result: A 77-year-old woman presented with neck and bilateral shoulder pain and stiffness. Her temperature was 37.6 °C. Blood testing revealed a mildly elevated inflammatory response, although blood cultures were negative. Computed tomography (CT) showed atlanto-axial subluxation and joint destruction. T2-weighted magnetic resonance imaging (MRI) displayed high signals in the soft tissues in the anterior space of the atlas and axis, posterior wall of the pharynx, and interspinous ligament, indicating spinal cord compression at the C1 level. Differential diagnoses of inflammation and diseases causing atlanto-axial subluxation included rheumatoid arthritis, amyloidosis, pyogenic spondylitis due to posterior pharyngeal abscess, and crowned dens syndrome. After the systematic elimination of each condition, we considered Grisel’s syndrome and began non-surgical treatment with intravenous antibiotics and a Philadelphia neck collar. Her inflammatory response and neck pain gradually decreased. Six months later, there was no progression of instability. She was able to walk unaided and live normally with the use of a neck collar as needed.Conclusion: Grisel’s syndrome occurs predominantly in children, but can also afflict adults. Since early diagnosis and treatment can improve symptoms in some cases and prevent progressive atlanto-axial instability, prompt evaluation of the atlanto-axial joint using CT or MRI is advised in patients with neck pain and limited range of motion. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
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