1. Co-metabolic degradation of iomeprol by a Pseudomonas sp. and its application in biological aerated filter systems.
- Author
-
Xu, Bingjie, Xu, Bin, Shan, Shiwei, Xue, Gang, Wang, Tianfeng, Qiu, Xiuwen, and Zhan, Changchao
- Subjects
RADIOGRAPHIC contrast media ,PSEUDOMONAS ,BIODEGRADATION ,SEWAGE purification ,BACTERIAL metabolism ,ELECTRON transport ,MICHAELIS-Menten equation ,GLUCOSE - Abstract
The non-ionic water-soluble X-ray contrast agent iomeprol (IOM) enters the water supply through sewage treatment plants, which can cause considerable environmental harm. In this study,Pseudomonassp. I-24 (I-24) was tested for its ability to remove IOM from water via co-metabolic pathways. The optimum removal rate of IOM by I-24 was 38.43% ± 3.70% when starch served as the source of external carbon, and its co-metabolism of IOM conformed to the first-order kinetics. The highest activity of intracellular enzyme (degrading enzyme) extracted from I-24 was 0.143 ± 0.005 mU in starch condition. The Michaelis constant of the degrading enzyme was found to be 91.08 μmol L−1. However, glucose and maltose showed the best promotive effects on the growth and electron transport activity of I-24, indicating that overgrowth may result in competitive inhibition and a reduced degradation rate of IOM. Adding I-24 and degrading enzymes to biological aerated filters increased IOM removal rates without affecting CODMnremoval. [ABSTRACT FROM PUBLISHER]
- Published
- 2018
- Full Text
- View/download PDF