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Since the first discovery of dinosaur eggs announced by the American Museum of Natural History (central Asiatic expeditions to Mongolia) in 1923, these fossils became widely recognised from Mesozoic strata in Asia and elsewhere. In the 1920s, and actually before 1923, dinosaur eggs had been collected from another part of Asia, specifically Liaoning Province in northeastern China. From 1921 to the late 1920s, five eggs were collected from the Cenomanian Quantou Formation during construction, and thus represent an important historic collection as some of the earliest dinosaur eggs discovered in Asia. Here, we describe one egg from this collection and assign it to Paraspheroolithus irenensis, an egg taxon that likely belonged to hadrosaur dinosaurs. The egg is approximately 7 × 8 cm in diameter, with eggshell that displays typical spheroolithid microstructure and is just over a millimetre in thickness. Paraspheroolithus irenensis is shown to be the most widespread spheroolithid oospecies in China, and our comparative work extends its geographic range to northeastern most China. The Quantou Formation thus has yielded at least three spheroolithid oospecies, indicating the potential for a diversity of hadrosaur or related ornithopod species in the region at that time. [ABSTRACT FROM AUTHOR]

Context: We recently reported that F2, an oligomer procyanidin fraction isolated from grape seeds, triggered an original form of cell death in U-87 human glioblastoma cells with a phenotype resembling morphological characteristics of paraptosis. However, the specific death mode induced by F2 and the mechanism of its action have not been assessed so far. Objective: In the present work, we therefore further investigated the death mode of human glioblastma cells induced by F2 and gained insight into the nature of the signaling pathways activated by F2 in glioblastoma cells. Materials and methods: Cell viability assay using MTT, (AO/EB) double staining, Western blot analysis, and Ca2+ assay using fura-2. Results: Morphology studies revealed extensive cytoplasmic vacuolization in dying cells and no apoptotic body formation, membrane bleb formation, or nuclear fragmentation, though some was accompanied by MAPK activation and new protein synthesis, and was independent of caspase activation. Moreover, we demonstrated the involvement of calcium mobilization in F2-induced U-87 cell signaling. Discussion and conclusion: Altogether we showed that F2 induced a kind of cell death resembling paraptosis in U-87 cells. The current report complements previous studies on the characterization of F2-induced U-87 cell death, enhances our understanding of the action mechanism of F2 on glioma, and helps in the development of novel antitumor therapeutics. [ABSTRACT FROM AUTHOR]