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The development of novel biocompatible and cost effective cryogel membrane which shows enhanced antimicrobial properties in order to use for several approaches such as wound dressing, scaffold or food packaging was aimed in this study. A super macro porous lysozyme imprinted cryogel membranes showing antibacterial effect against both Gram-positive and Gram-negative bacteria were prepared by using molecular imprinting technique. N-methacryloyl-(L)-histidine methyl ester (MAH) was used as the pseudo specific ligand and complexed with Cu ++ in order to provide metal ion coordination between MAH and template molecule (lysozyme). Comparing the antibacterial activity of different lysozyme concentrations, cryogel membranes were prepared in three different concentrations. To synthesize Poly (hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methylester) P(HEMA-MAH) cryogel membrane, free radical polymerization initiated by N, N, N', N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) was carried out at -12 °C. The characterization of the lysozyme imprinted cryogel membrane was accomplished by using scanning electron microscopy (SEM), swelling degree measurements and Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) spectroscopy. The cytotoxicity test of produced membrane was performed by using mouse fibroblast cell line L929. The antibacterial activity of P(HEMA-MAH) lysozyme molecular imprinted [P(HEMA-MAH) Lyz-MIP] cryogel membranes against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ) were determined by Kirby-Bauer membranes diffusion and viable cell counting methods. When the antibacterial effect of P(HEMA-MAH) Lyz-MIP cryogel membranes were evaluated, it was found that P(HEMA-MAH) Lyz-MIP cryogel membranes had stronger antibacterial effects against Gram-negative E. coli bacteria even in low lysozyme concentrations. In addition, 100% bacterial inhibition was detected for both of two bacteria at increasing lysozyme concentrations.

The aim of this work is to develop drug carrier system with high loading capacity and controlled drug release profile for antibiotic release. For this purpose, composite molecularly imprinted nanofibers were prepared via in-situ graft polymerization of methacrylic acid as a monomer, N,N'-methylene bisacrylamide as a crosslinker and gentamicin sulfate as a template molecule onto surface-modified bacterial cellulose nanofibers. Gentamicin imprinted microparticles were fabricated onto bacterial cellulose nanofibers resulting in the formation of composite BC nanofibers. Thus, the composite nanofibers incorporated with gentamicin imprinted microparticles were achieved to fabricated. The in-vitro drug release tests were performed to evaluate the release performance of the resultant composite nanofibers at 37 °C. Also, kinetic models were applied to the drug release data. It was determined that the drug release from the composite molecularly imprinted nanofibers fit well in the Korsmeyer-Peppas model.