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Your search keyword '"Rahmati, Mehdi"' showing total 2 results
Start Over Author "Rahmati, Mehdi" Publisher tehran university of medical sciences
2 results on '"Rahmati, Mehdi"'

1. Resistance to Antimicrobial Agents, Chemical Disinfectants and Distribution of Effectors Proteins of Type Three Secretion System in Pseudomonas aeruginosa Isolated From Burn and Hospital Environments in South East of Iran.

Author

Rahmati, Mehdi, Mansouri, Shahla, and Sharifi, Hossein

Subjects
*DRUG resistance in microorganisms, *ANTI-infective agents, *DISINFECTION & disinfectants, *PSEUDOMONAS aeruginosa, *POLYMYXIN B, *PSEUDOMONADACEAE, *PSEUDOMONAS syringae
Abstract

Pseudomonas aeruginosa (P. aeruginosa) is a common bacteria associated with burn infections and resistance to a wide range of disinfectants and antimicrobial agents which is able to produce different virulence factors. In this study, the susceptibility of P. aeruginosa isolates from the burn (burn=57) and hospital environment (HE=19) to antimicrobial agents and chemical disinfectants was determined by disc and well diffusion agar method, respectively. The results showed 100% sensitivity to polymyxin B, while sensitivity to other agents was low and ranged from 40.8% for imipenem and amikacin to 6.6% for ceftizoxime. Among the disinfectant used, the mean diameter of inhibition zones (DIZ) was higher for Deconex, while nitrofurazone had the lowest DIZ. In most cases, the HE isolates were significantly more susceptible to disinfectants and antimicrobial agents compared to burn isolates (P=0.01). The genes for the exoenzyme T, Y, U, and S were detected in 100%, 89.8%, 43.4%, and 48.7% of the isolates, respectively. The prevalence of exo U and exoY was significantly higher in the burn isolates compared to HE isolates (P=0.001). The results of this study indicate a significantly higher level of resistance against the majority of the antimicrobial agents in the burn isolates compared to HE isolates, which was significantly higher than the environmental isolates. The prevalence of T3SS effectors proteins and their pattern were also different in the burn, and the HE isolates, indicating a divergence in pathogenicity of the burn isolates from those of the environmental isolates. [ABSTRACT FROM AUTHOR]

Published
2020

2. Study on imipenem resistance and prevalence of blaVIM1 and blaVIM2 metallo-beta lactamases among clinical isolates of Pseudomonas aeruginosa from Mashhad, Northeast of Iran

Author

Mirbagheri, Seyedeh Zohreh, Meshkat, Zahra, Naderinasab, Mahboubeh, Rostami, Sina, Nabavinia, Maryam Sadat, and Rahmati, Mehdi

Subjects
Imipenem, Pseudomonas aeruginosa, lcsh:QR1-502, Original Article, Metallobeta-lactamase genes, lcsh:Microbiology
Abstract

Background and Objectives: The main cause of serious nosocomial infections is a Gram-negative pathogen known as Pseudomonas aeruginosa (P. aeruginosa). Carbapenems are widely used as an appropriate treatment for these infections, however resistance to these agents has been observed and is increasing. Metallo beta-lactamase (MBLs) enzyme is one of the main causes of resistance to carbapenem. In the current study the frequency and production of VIM1 and VIM2 by imipenem-resistant P. aeruginosa isolates of patients hospitalized in Imam Reza hospital were evaluated. Materials and Methods: In this study, 131 clinical samples were collected from patients hospitalized in Imam Reza hospital in Mashhad during a 15-month period from May 2011 to November 2012. After verification of P. aeruginosa isolates, antibiotic resistance patterns of isolates were determined for 14 antibiotics by Kirby-Bauer standard disk diffusion according to the CLSI guidelines. Combined-disk test was used for phenotypic determination of MBLs-producing isolates and after DNA extraction, genotypic determination of VIM1 and VIM2 metallo beta-lactamase genes was carried out using Multiplex- PCR. Results: Of 63 imipenem-resistant isolates (48.5%), 56 (88.8%) were MBL-producing in phenotypic assessments. Also amongst imipenem-resistant isolates, the frequency of VIM1 and VIM2 genes were 58.7 and 3.17%, respectively. Conclusion: The results of the current study along with the results of the other conducted studies in Iran in recent years demonstrate that the average resistance to imipenem in P. aeruginosa isolates was 51.3% which has increased in comparison with the results in 2006 (32.9%). It was also determined that the frequency of VIM1 gene was more than VIM2 gene. In phenotypic assessment by using CD method, 49.6% of isolates were determined as MBLs-producing. The sensitivity and specificity of this method were verified in comparison with the results of PCR test.

Published
2015

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