1. Transcriptome analysis of Zi geese (Anser cygnoides) prehierarchical follicles and verification of related genes in arginine metabolic pathway.
- Author
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Yongfeng Sun, Lulu Liu, Chang Liu, Sello, Cornelius Tlotliso, Huiyan Wu, Hongtao Lu, Chenguang Xu, and Yujian Sui
- Subjects
ARGININE metabolism ,TRANSCRIPTOMES ,SWAN goose ,GENE expression ,GENE ontology - Abstract
Arginine metabolism plays a pivotal role in the mammalian reproductive system by controlling various physiological processes of the reproductive organs including follicular development, ovulatory mechanisms and egg production. However little is known about the molecular mechanisms regulating arginine metabolism at early stages of folliculogenesis. The objective of the current study was to determine the arginine metabolic pathways in prehierarchical follicles of Zi geese through the Illumina Hiseq 2500 platform and their transcripts through the de novo assembly. In this study, five different stages of the geese prehierarchical follicles transcriptome were sequenced. A total of 31722729162 bp from 224929214 high quality clean sequences was obtained. There were 74.75% transcripts annotated pathways, and 106 unigenes were mapped in the Arginine and proline metabolism pathway. Combined with Gene Ontology (GO) functions, ArgE, NOS and ODC were selected as the candidate genes to explore the arginine metabolic pathway and determine their relative expression in the prehierarchical follicles of geese. The results showed that the three genes were expressed in all five prehierarchical stages of geese follicles, with the predominant expression in primary follicles (p < 0.05), indicating promotion of follicular development. This study provides useful inclusive gene expression profiling information that would certainly provide basic theory for future research studies on follicular development in Zi geese. [ABSTRACT FROM AUTHOR] more...
- Published
- 2019