1. miR-203 regulates progenitor cell proliferation during adult zebrafish retina regeneration
- Author
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Kamya Rajaram, James G. Patton, Rachel L. Harding, and David R. Hyde
- Subjects
Microinjections ,Blotting, Western ,Pax6b ,Biology ,Real-Time Polymerase Chain Reaction ,Article ,Retina ,Statistics, Nonparametric ,Morpholinos ,Asymmetric cell division ,medicine ,Animals ,Regeneration ,Progenitor cell ,Cloning, Molecular ,Zebrafish ,Molecular Biology ,Progenitor ,Retinal regeneration ,Cell Proliferation ,Reverse Transcriptase Polymerase Chain Reaction ,Regeneration (biology) ,Stem Cells ,Retina regeneration ,Cell Biology ,miR-203 ,biology.organism_classification ,Flow Cytometry ,Immunohistochemistry ,Cell biology ,Endothelial stem cell ,MicroRNAs ,medicine.anatomical_structure ,Electroporation ,Gene Expression Regulation ,Progenitor cell proliferation ,Muller glia ,Developmental Biology - Abstract
Damage of the zebrafish retina triggers a spontaneous regeneration response that is initiated by Müller Glia (MG) dedifferentiation and asymmetric cell division to produce multipotent progenitor cells. Subsequent expansion of the progenitor pool by proliferation is critical for retina regeneration. Pax6b expression in the progenitor cells is necessary for their proliferation, but exact regulation of its expression is unclear. Here, we show that miR-203 is downregulated during regeneration in proliferating progenitor cells. Elevated miR-203 levels inhibit progenitor cell expansion without affecting MG dedifferentiation or progenitor cell generation. Using GFP-reporter assays and gain and loss of function experiments in the retina, we show that miR-203 expression must be suppressed to allow pax6b expression and subsequent progenitor cell proliferation.
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