1. Chemical Constituents of the Culture Broth of Phellinus linteus and Their Antioxidant Activity
- Author
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Myeong-Seok Lee, Byung Soon Hwang, Bong-Sik Yun, Geon-Sik Seo, and In-Kyoung Lee
- Subjects
0301 basic medicine ,Antioxidant ,Inotilone ,medicine.medical_treatment ,Ethyl acetate ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Column chromatography ,Caffeic acid ,medicine ,Chromatography ,biology ,Traditional medicine ,Medicinal fungus ,030108 mycology & parasitology ,biology.organism_classification ,Phellinus linteus ,030104 developmental biology ,Infectious Diseases ,chemistry ,Sephadex ,Chemical constituents ,Research Article - Abstract
The medicinal fungus Phellinus linteus, in the family Hymenochaetaceae, has been used as a traditional medicine for the treatment of various diseases. In this study, the chemical constituents of the culture broth of P. linteus were investigated. P. linteus was cultured in potato dextrose broth medium, and the culture broth was extracted with ethyl acetate. The ethyl acetate-soluble portion was concentrated and subjected to ODS column chromatography, followed by Sephadex LH-20 column chromatography. Six compounds (1~6) were purified by preparative reversed-phase high-performance liquid chromatography. Spectroscopic methods identified their structures as caffeic acid (1), inotilone (2), 4-(3,4-dihydroxyphenyl)-3-buten-2-one (3), phellilane H (4), (2E,4E)-(+)-4'-hydroxy-γ-ionylideneacetic acid (5), and (2E,4E)-γ-ionylideneacetic acid (6). Compounds 1, 2, and 3 exhibited potent dose-dependent antioxidant activity.
- Published
- 2015