1. In vivo quantification of optical contrast agent dynamics in rat tumors by use of diffuse optical spectroscopy with magnetic resonance imaging coregistration
- Author
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Jun Wang, Bruce J. Tromberg, Gultekin Gulsen, Frédéric Bevilacqua, Sean Merritt, David J. Cuccia, Anthony J. Durkin, Hon Yu, and Orhan Nalcioglu
- Subjects
Indocyanine Green ,Optics and Photonics ,Skin Neoplasms ,genetic structures ,Materials Science (miscellaneous) ,media_common.quotation_subject ,Contrast Media ,Vascular permeability ,Adenocarcinoma ,Industrial and Manufacturing Engineering ,chemistry.chemical_compound ,Nuclear magnetic resonance ,In vivo ,medicine ,Extracellular ,Animals ,Contrast (vision) ,Business and International Management ,Coloring Agents ,media_common ,medicine.diagnostic_test ,Chemistry ,Magnetic resonance imaging ,Blood flow ,Magnetic Resonance Imaging ,Rats, Inbred F344 ,eye diseases ,Diffuse optical imaging ,Rats ,Methylene Blue ,Indocyanine green ,Neoplasm Transplantation - Abstract
We present a study of the dynamics of optical contrast agents indocyanine green (ICG) and methylene blue (MB) in an adenocarcinoma rat tumor model. Measurements are conducted with a combined frequency-domain and steady-state optical technique that facilitates rapid measurement of tissue absorption in the 650–1000-nm spectral region. Tumors were also imaged by use of contrast-enhanced magnetic resonance imaging (MRI) and coregistered with the location of the optical probe. The absolute concentrations of contrast agent, oxyhemoglobin, deoxyhemoglobin, and water are measured simultaneously each second for approximately 10 min. The differing tissue uptake kinetics of ICG and MB in these late-stage tumors arise from differences in their effective molecular weights. ICG, because of its binding to plasma proteins, behaves as a macromolecular contrast agent with a low vascular permeability. A compartmental model describing ICG dynamics is used to quantify physiologic parameters related to capillary permeability. In contrast, MB behaves as a small-molecular-weight contrast agent that leaks rapidly from the vasculature into the extravascular, extracellular space, and is sensitive to blood flow and the arterial input function. © 2003 Optical Society of America.
- Published
- 2003