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As manufacturing and service integrate, service manufacturing has been a new trend in the revolution of manufacturing enterprise. Based on value evolution mechanism, this essay puts main efforts in analyzing the elements, structures and development trend of service manufacturing, and advances a three-layer value chain model based on service production activities, productive service activities and customers' all way participation. Lastly, this essay establishes a three-layer value-added model of service manufacturing value chain, and puts forward six key factors to realize value-added of service manufacturing value chain.

Aiming to the difficulty of washing-hand for a one-armed man, functional requirements of washing-hand for a one-armed man is analyzed, and TRIZ theory is described in detail. A novel conceptual assisted washing-hand device for a one-armed man is put forward in this paper. Innovative problems of the assisted washing-hand device design were solved based on contradiction conflict matrix theory and material field theory of TRIZ. The whole three-dimensional model of assisted washing-hand device is built. TRIZ theory can provide the reference basis for structural design of assisted washing-hand device for a one-armed man.

Activated ozone as the carrier，crylic acid as the monomer and glutaraldehyde as the cross-linking agent were used to research the immobilization of transglutaminase on polypropylene microporous membranes. The effect of ozone activation time，grafting time，grafting temperature，monomer concentration and ammonium ferrous sulfate concentration on grafting degree were studied, the Immobilization conditions were also studied. We found the optimal reaction conditions as following: consistency of hexamethylendiamine was 15%，at 50°C for 120min and consistency，at 30°C for 45minites，consistency of enzyme was 15mg/mL at 4°C. So the immobilized amount of protein could come to 30.23mg/g membrane and the activity of immobilized MTG come to 16.9U/g membrane.

Laser induced fluorescence (LIF) detection is one of the main means of Capillary Electrophoresis (CE) chip detection, in which the confocal detecting device is commonly used for its higher sensitivity and signal-to-noise ratio (SNR). Based on confocal LIF detection principle, the confocal laser induced fluorescence detecting system, which could realize the auto focusing, and auto tracking was presented, and it contained the confocal optical system, the microprocessor control system and the computer process system. This device can acquire the fluorescence data by PMT or the chip images by CCD, and 3-dimensional electric moving stage could be controlled to accomplish the auto focusing and auto tracking by image process. The device could detect or observe the CE chip data in real time.