1. DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron depletion
- Author
-
Long, Maeve, Sanchez-Martinez, Alvaro, Longo, Marianna, Suomi, Fumi, Stenlund, Hans, Johansson, Annika I, Ehsan, Homa, Salo, Veijo T, Montava-Garriga, Lambert, Naddafi, Seyedehshima, Ikonen, Elina, Ganley, Ian G, Whitworth, Alexander J, McWilliams, Thomas G, STEMM - Stem Cells and Metabolism Research Program, Faculty of Medicine, Medicum, University of Helsinki, Research Programs Unit, Department of Anatomy, Lipid Trafficking Lab, Long, Maeve [0000-0001-5146-0750], Sanchez-Martinez, Alvaro [0000-0002-2728-6251], Suomi, Fumi [0000-0001-8883-3338], Stenlund, Hans [0000-0001-9943-296X], Johansson, Annika I [0000-0001-5000-1288], Ehsan, Homa [0000-0001-5252-4015], Salo, Veijo T [0000-0002-6991-2142], Montava-Garriga, Lambert [0000-0003-0025-5700], Naddafi, Seyedehshima [0000-0001-6187-1967], Ikonen, Elina [0000-0001-8382-1135], Ganley, Ian G [0000-0003-1481-9407], Whitworth, Alexander J [0000-0002-1154-6629], McWilliams, Thomas G [0000-0002-9570-4152], and Apollo - University of Cambridge Repository
- Subjects
Cellbiologi ,Ubiquitin-Protein Ligases ,GLYCOLYSIS ,ENDOPLASMIC-RETICULUM ,lipid droplet ,General Biochemistry, Genetics and Molecular Biology ,iron ,MITOCHONDRIA ,Animals ,OXIDATIVE STRESS ,Molecular Biology ,ULK1 ,DGAT1 ,Mammals ,General Immunology and Microbiology ,General Neuroscience ,OPHTHALMIC ACID ,Cell Biology ,mitophagy ,MITO-QC ,LIPID DROPLETS ,1182 Biochemistry, cell and molecular biology ,AUTOPHAGY ,FATTY-ACIDS ,Lysosomes ,Protein Kinases ,metabolism - Abstract
Funder: Novo Nordisk Foundation/Novo Nordisk Fonden; Id: http://dx.doi.org/10.13039/501100011747, Funder: Helsingin Yliopisto (HY); Id: http://dx.doi.org/10.13039/100007797, Funder: Sydäntutkimussäätiö (Finnish Foundation for Cardiovascular Research); Id: http://dx.doi.org/10.13039/501100005633, Funder: Jane and Aatos Erkko Foundation; Id: http://dx.doi.org/10.13039/501100004012, Mitophagy removes defective mitochondria via lysosomal elimination. Increased mitophagy coincides with metabolic reprogramming, yet it remains unknown whether mitophagy is a cause or consequence of such state changes. The signalling pathways that integrate with mitophagy to sustain cell and tissue integrity also remain poorly defined. We performed temporal metabolomics on mammalian cells treated with deferiprone, a therapeutic iron chelator that stimulates PINK1/PARKIN-independent mitophagy. Iron depletion profoundly rewired the metabolome, hallmarked by remodelling of lipid metabolism within minutes of treatment. DGAT1-dependent lipid droplet biosynthesis occurred several hours before mitochondrial clearance, with lipid droplets bordering mitochondria upon iron chelation. We demonstrate that DGAT1 inhibition restricts mitophagy in vitro, with impaired lysosomal homeostasis and cell viability. Importantly, genetic depletion of DGAT1 in vivo significantly impaired neuronal mitophagy and locomotor function in Drosophila. Our data define iron depletion as a potent signal that rapidly reshapes metabolism and establishes an unexpected synergy between lipid homeostasis and mitophagy that safeguards cell and tissue integrity.
- Published
- 2022