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Submitted by Bruno Matos Nascimento (brunomatos@uefs.br) on 2020-06-10T21:33:05Z No. of bitstreams: 1 Alan Ara?jo de Jesus - Tese.pdf: 6633416 bytes, checksum: b185bad02b72978e730c00a1cadef65c (MD5) Made available in DSpace on 2020-06-10T21:33:05Z (GMT). No. of bitstreams: 1 Alan Ara?jo de Jesus - Tese.pdf: 6633416 bytes, checksum: b185bad02b72978e730c00a1cadef65c (MD5) Previous issue date: 2010-11-07 Although the bone tissue has a good regenerative capacity, there are defects which need bone grafting in order to properly recover the damaged tissue. Autogenous bone graft, the ?gold standard? procedure, requires a second operation, with increased complexity and morbidity. In this study we investigated the osteogenic potential of stem cells obtained from the pulp of deciduous teeth (DSC) associated with biomaterials. We evaluated the effects of three biomaterials [Bone Ceramic (BC), Bonefill and a polyurethane derived from Ricinus communis (RC)] on in vitro cultures of DSC on cell proliferation, osteogenic differentiation (after staining with alizarin red), and cell morphology (by optical microscopy) and the three biomaterials were analyzed by scanning electron microscopy (SEM), and chemical composition [by electron dispersive spectroscopy (EDS)]. The DSC were easily obtained and showed good proliferative capacity. The BC stimulated cell proliferation and none of the biomaterials inhibited osteogenic differentiation. In the in vivo study, rats were divided into three groups and bone defects were prepared in the calvaria. The control group was filled with clot and the others were filled with the RC or the RC+DSC. After 15, 30 and 60 days they were sacrificed and the defect area was analyzed by digital radiography, optical microscopy, SEM, and EDS. There was no spontaneous regeneration of the defect. The group RC+DSC showed highest radiopacity at 15 days. The biomaterial worked as a mechanic filling and showed biocompatibility, evidence of osseointegration and no particles re-absorption. Only sparse ossified areas were identified which in the group RC+DSC were larger and more mature. Although the osteoinductive or osteogenic potential of RC+DSC was not clearly demonstrated, the association of cell therapy with biomaterials is promising and should be further investigated. O tecido ?sseo tem boa capacidade de regenera??o, por?m existem defeitos onde ? necess?ria a enxertia ?ssea. O enxerto aut?geno ? o padr?o ouro, mas causa uma segunda ferida cir?rgica e maior complexidade e morbidade. Neste trabalho investigamos o potencial osteog?nico de c?lulas-tronco da polpa de dentes dec?duos (CTD) associadas a biomateriais. Foi avaliada in vitro a influ?ncia dos biomateriais Boneceramic (BC), Bonefill e Composto ?sseo de r?cinus (COR) sobre culturas de CTD, em rela??o ? prolifera??o celular e ? diferencia??o osteog?nica, corando-se com alizarina vermelha, al?m da an?lise morfol?gica por microscopia ?tica e eletr?nica de varredura (MEV) e an?lise de elementos qu?micos, por espectroscopia de dispers?o de el?trons (EDS). As CTD foram facilmente obtidas e apresentaram boa capacidade proliferativa. O BC estimulou a prolifera??o celular e nenhum dos biomateriais inibiu a diferencia??o osteog?nica. No estudo in vivo, ratos foram divididos em tr?s grupos e submetidos ? indu??o de defeito na calv?ria. O grupo controle foi preenchido com co?gulo e os demais receberam o COR, ou o COR+CTD. Ap?s 15, 30 e 60 dias foram feitas an?lises por radiografias digitais, microscopia ?tica, MEV e EDS. N?o ocorreu regenera??o espont?nea do defeito. O grupo COR+CTD exibiu maior radiopacidade aos 15 dias. O biomaterial n?o apresentou reabsor??o, foi biocompat?vel, com ind?cios de osseointegra??o, e funcionou como preenchimento mec?nico. Somente foram identificadas ?reas ossificadas esparsas, que no grupo COR+CTD foram maiores e mais maturadas. Apesar de n?o ter sido comprovada uma a??o osteoindutora ou osteog?nica do COR+CTD, a associa??o da terapia celular a biomateriais ? promissora e deve ser melhor investigada.

Submitted by Bruno Matos Nascimento (brunomatos@uefs.br) on 2021-11-08T22:27:03Z No. of bitstreams: 1 Maros_Capa e Prefaciais[23.08.08] 1-mesclado.pdf: 683810 bytes, checksum: 1600dd66ca1579f0d955ddeb3b22fcc8 (MD5) Made available in DSpace on 2021-11-08T22:27:04Z (GMT). No. of bitstreams: 1 Maros_Capa e Prefaciais[23.08.08] 1-mesclado.pdf: 683810 bytes, checksum: 1600dd66ca1579f0d955ddeb3b22fcc8 (MD5) Previous issue date: 2008-10-09 Addresses cell therapy for transplant autologo of stem cells from bone marrow in patients with parenchymal disease chronic liver. To evaluate the safety and feasibility of bone marrow cell (BMC) transplantation in patients with chronic liver disease on the waiting list for liver transplantation. Ten patients (eight males) with chronic liver disease were enrolled to receive infusion of autologous bone marrow-derived cells. Seven patients were classified as Child-Pugh B and three as Child-Pugh C. Baseline assessment included complete clinical and laboratory evaluation and abdominal MRI. Approximately 50 mL of bone marrow aspirate was prepared by centrifugation in a ficoll-hypaque gradient. At least of 100 millions of mononuclear-enriched BMCs were infused into the hepatic artery using the routine technique for arterial chemoembolization for liver tumors. Patients were followed up for adverse events up to 4 mo. The results of median age of the patients was 52 years (range 24-70 years). All patients were discharged 48 h after BMC infusion. Two patients complained of mild pain at the bone marrow needle puncture site. No other complications or specific side effects related to the procedure were observed. Bilirubin levels were lower at 1 (2.19 ? 0.9) and 4 mo (2.10 ? 1.0) after cell transplantation that baseline levels (2.78 ? 1.2). Albumin levels 4 mo after BMC infusion (3.73 ? 0.5) were higher than baseline levels (3.47 ? 0.5). International normalized ratio (INR) decreased from 1.48 (SD = 0.23) to 1.43 (SD = 0.23) one month after cell transplantation. Concluded that BMC infusion into hepatic artery of patients with advanced chronic liver disease is safe and feasible. In addition, a decrease in mean serum bilirubin and INR levels and an increase in albumin levels are observed. Our data warrant further studies in order to evaluate the effect of BMC transplantation in patients with advanced chronic liver disease. Este estudo aborda a terapia celular pelo transplante aut?logo de c?lulas-tronco de medula ?ssea em pacientes com doen?a parenquimatosa cr?nica de f?gado. Tem por objetivo avaliar a seguran?a e exeq?ibilidade do transplante de c?lulas mononucleares da medula ?ssea (CMO) em pacientes com doen?a cr?nica de f?gado em lista de transplante hep?tico. Dez pacientes (oito masculinos) com doen?a cr?nica de f?gado foram inclu?dos no estudo e receberam infus?o de c?lulas aut?logas provenientes da medula ?ssea. Sete pacientes foram classificados como Chil-Pugh B e tr?s como Child-Pugh C. A confec??o da linha de base incluiu avalia??o clinica e laboratorial completa e resson?ncia magn?tica de abdome. Aproximadamente 50 ml aspirado da medula ?ssea foram preparados com centrifuga??o em gradiente de Ficoll-hypaque. No m?nimo 100 milh?es de c?lulas mononucleares da medula ?ssea foram infundidas na art?ria hep?tica usando a t?cnica rotineira de quimioemboliza??o para tumores hep?ticos. Os pacientes foram acompanhados quanto a efeitos adversos por quatro meses. Os resultados indicaram que a m?dia de idade dos pacientes foi de 52 anos (varia??o entre 24 e 70 anos). Todos os pacientes obtiveram alta com 48 horas ap?s a infus?o de c?lulas da medula ?ssea. Dois pacientes se queixaram de dor moderada em sitio de pun??o da medula ?ssea. Nenhuma outra complica??o ou efeito adverso relacionados ao procedimento foram observados, os n?veis de bilirrubina foram menores em um m?s (2.19 ? 0.9) e quatro meses (2.10 ? 1.0) ap?s o transplante de c?lulas em rela??o aos n?veis de base (2.78 ? 1.2). Os n?veis de albumina quatro meses ap?s a infus?o de c?lulas da medula ?ssea (3.73 ? 0.5) foram maiores de que os n?veis basais (3.47 ? 0.5). Rela??o de Normalidade Internacional (RNI) decresceu de 1.48 (DP = 0.23) para 1.43 (DP = 0.23) um m?s ap?s o transplante celular. Concluiu-se que a infus?o de c?lulas da medula ?ssea na art?ria hep?tica de pacientes com doen?a cr?nica avan?ada de f?gado ? segura e exeq??vel. Adicionalmente, houve um decr?scimo na media dos n?veis de bilirrubina s?rica e RNI e um aumento nos n?veis de albumina. Os dados aqui apresentados permitem que outros estudos possam vir a avaliar a efic?cia do transplante de c?lulas da medula ?ssea em pacientes com doen?a cr?nica de f?gado.

Submitted by Bruno Matos Nascimento (brunomatos@uefs.br) on 2021-05-20T21:35:34Z No. of bitstreams: 1 Juliana Fraga Vasconcelos - Disserta??o.pdf: 1671955 bytes, checksum: c802fb62f37ddb9e7f310ede6c6df750 (MD5) Made available in DSpace on 2021-05-20T21:35:35Z (GMT). No. of bitstreams: 1 Juliana Fraga Vasconcelos - Disserta??o.pdf: 1671955 bytes, checksum: c802fb62f37ddb9e7f310ede6c6df750 (MD5) Previous issue date: 2007-07-26 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES Asthma is a chronic inflammation of airways associated with a Th2 immune response, involving eosinophils, mast cells, IgE and several soluble mediators. The available drugs are not very efficient, being the corticosteroids the most used, despite their side effects. In this work we investigated the efficacy of four pure substances, isolated from plants found in the brazilian semi-arid region, in the treatment of bronchic asthma in BALB/c mice immunized with ovalbumin. Treatment with physalins B or F did not revert the inflammatory response. In contrast, the administration of lupeol or umbeliferona caused the reduction of eosinophils and other inflammatory cells in the lung and of the production of mucus and pro-inflammatory cytokines (IL-4, IL-5 and IL-13) similar to the effect of dexamethasone. Our findings reinforce the importance of the brazilian flora as a rich source of biomolecules. The investigation of the mechanisms of action of these molecules may contribute for the development of new drugs for the treatment of this pathology. A asma ? uma inflama??o cr?nica das vias a?reas que est? associada a resposta imune Th2, com participa??o de eosin?filos, mast?citos, IgE e v?rios mediadores sol?veis. Os f?rmacos dispon?veis n?o possuem uma alta efic?cia, sendo os cortic?ides os mais utilizados, apesar de possu?rem efeitos colaterais indesej?veis. Neste trabalho foi investigada a efic?cia de quatro mol?culas purificadas a partir de plantas do semi-?rido brasileiro no tratamento da asma br?nquica em camundongos BALB/c imunizados com ovalbumina. O tratamento com as fisalinas B e F (2 mg/animal) n?o reverteu o quadro inflamat?rio. J? a administra??o de umbeliferona ou de lupeol (2 mg/animal) causou uma redu??o de eosin?filos e outras c?lulas inflamat?rias no pulm?o e da produ??o de muco e de citocinas pr?-inflamat?rias (IL-4, IL-5 e IL-13), semelhante a dexametasona. Esses achados enfatizam a import?ncia da flora brasileira como fonte rica de biomol?culas. A investiga??o dos mecanismos de a??o dessas mol?culas poder? guiar o desenvolvimento de novos f?rmacos que intervenham nessa patologia.