Search

The high incidence of breast cancer cases in the world requires the use of applicative methods. The 7,12-dimethylbenz(a)anthracene (DMBA) induced breast cancer animal model is a widely used chemical-induced animal models for research on breast cancer. However, the molecular mechanism related to DMBA induction remains unclear. Good understanding on DMBA-induced animal models is crucial for studies related to future breast cancer treatments as animal models will provide a deeper understanding of anticancer medication, specifically those aimed for treating breast cancer. The aim of this study was to develop an DMBA-induced animal model for breast cancer. This study used female Wistar rats injected subcutaneously with DMBA as a carcinogen-induced agent (20 mg/kg) to induce tumor. Rat tumors were then evaluated and breast appearance was observed weekly, starting from day 28th after DMBA injection. Breast cancer tissue was then sampled and stored at -80°C until it was used for western blot and histological study. This study indicated that DMBA induced cancer in female Wistar rat’s breasts, and cytoplastic cells and lung metastatic was identified macroscopically and histopathologically. The metabolic sign was observed in the lung and breast sections. Interestingly, the DMBA induction in this study does not only induce organ cancers but also induces estrogen receptors and stimulates signaling of estrogen receptors α (ERα), ERβ, and Akt.

Exercise could delay the degeneration process by increasing Klotho, which is an endogen antioxidant. Klotho protects against oxidative damages, regulates hippocampal synaptic plasticity, and enhances GluN2B expression. This study aimed to analyze the effect of 14-day and 8-week moderate-intensity exercise on Klotho and GluN2B mRNA expressions in rat hippocampus. This study was an animal experimental study using Wistar rats that was performed from June to September 2019 at the Central Laboratory, Universitas Padjadjaran, Indonesia. Rats were divided into 4 groups: control (14-day control and 8-week control) and exercise group (14-day exercise and 8-week exercise). The exercise groups ran at a moderate intensity (20m/min) for 30min/day, 5x/week. Hippocampus was then subjected to klotho and GluN2B gene expression analysis. The exercise groups showed a higher Klotho expression, both in 14 days (0.844±0.124 vs. 1.057±0.013) and 8 weeks (0.897±0.072 vs. 1.380±0.168) although statistically insignificant (p=0.24). No difference in GluN2B gene expression was observed between control and exercise groups on 14 days (1±0.09 vs. 1.22±0.09) and 8 weeks (1.24±0.03 vs. 1.38±0.05; p=0.11). In conclusion, 14-day and 8-week moderate-intensity exercise did not alter Klotho and GluN2B expressions in rat hippocampus.

A myokine is one of the proteins that are produced and released by myocytes in response to muscular contractions when doing physical exercise. One protein that is thought to function as myokine is FGF23.The purpose of this study was to determine the effect of acute physical exercise with moderate intensity on the expression of FGF23 gene in Wistar rat heart. This was an animal experimental study using 24 male Wistar rats that were divided into 4 groups:treatment groups that performed 30 minute acutephysical exercise with moderate intensity (20 m/min) for 3 days, 6 days, and 15 days and a control group without physical exercise. The study was conducted in the Animal Laboratory and Central Laboratory of Universitas Padjadjaran during the period of February to July 2019. Data observed were the FGF23 gene expressions in Wistar rats heart. Data were analyzed using Kruskal-Wallis and Mann-Whitney tests. The results from the Kruskal-Wallis test showed that acute physical exercise with moderate intensity did not increase the FGF23 gene expression in Wistar rat heart (p>0.05), and the average of relative ratios of FGF23/GAPDH gene expression were as follows: control (0.970±0,03), 3 days (0.992±0.03), 6 days (1.014±0.05), and 15 days (1.056±0.02). GAPDH was used in this study as a housekeeping gene since its expression is very constant. This study proves that FGF23 is more likely to take a role in the cardiac remodeling process, especially those associated with cardiac hypertrophy after chronic exercise with no effect observed after acute physical exercise with moderate intensity in Wistar rat heart.

Hypertension is a condition of persistently high blood pressure. It is currently a big health issue as its prevalence is high in Indonesia and its complications are numerous and deadly. Salt intake is one of the modifiable factors of hypertension. According to a study by Indonesian Ministry of Health, salt consumption in Indonesia is almost two times greater than the recommended salt diet from WHO. Sodium reabsorption in kidney plays a role in regulating blood pressure. Epithelial sodium channel (ENaC) is one of the structures that function in sodium reabsorption in kidney tubules. This study was conducted at the Central Laboratory and Animal Laboratory of the Faculty of Medicine Universitas Padjadjaran from June to Desember 2018. The aim of this study was to analyze the effect of high sodium diet on the expression of ENaC gene in kidney tubules of rats. Twelve Rattus norvegicus wistar rats were divided into two groups of control and treatment. Treatment group was given daily 2 mL NaCl solution treatment using gavage for 8 weeks. The expression of ENaC α, β, and γ was obtained by running tissue samples from kidney cortex and medulla in polymerase chain reaction and electrophoresis. The result showed that there was an insignificant decrease in ENaC α, β, and γ gene expressions in both kidney cortex and medulla of the treatment group when compared to control groupThis study concludes that ENaC gene expression is not significantly affected by high sodium diet.

Aerobic physical activity is known to induce skeletal muscle adaptation. Some genes, including MyoD, are known to have a major role in the process of muscle adaptation. Several studies has stated that the expression of MyoD increases during the aerobic activity process; however, the studies were carried out in a single bout. Studies on the effects of acute phase exercise (

Fitness becomes an important benchmark for public health in every age group. Activities with poor adverse fitness are at risk of injury. Good fitness is gained from a balance in everyday physical activity with conditions that are free of metabolic syndrome. This study investigated the relationship of physical activity, energy balance, and metabolic syndrome to the fitness of 166 professors of Universitas Padjadjaran who underwent a 1-month fitness checkup at BAPELKES Bandung in the period of 2017. Based on the regression analysis, the results showed a significant relationship between physical activity, energy balance, and metabolic syndrome on the physical fitness of the elderly group (F = 25.9290> F table = 2.427) with normal data distribution (Kolmogorov-Smirnov test). The results also showed a moderate relationship (R = 0.569 and R2 = 0.324). It also partially showed significant influence between physical activity and fitness (t = 8.541> t table = 1.975), significant influence between energy balance and physical fitness (t = 2.248> t table = 1.975), and no significant partial influence between metabolic syndrome and physical fitness of the elderly group (t = -0.029 Ftable=2,427) dengan distribusi data normal (tes Kolmogorov-Smirnov). Hasil juga menunjukkan hubungan yang moderat (R= 0,569 dan R2= 0,324). Secara parsial juga menunjukkan pengaruh yang signfikan aktivitas fisik dengan kebugaran (t= 8.541> t tabel= 1.975), pengaruh signifikan keseimbangan energi dengan kebugaran fisik (t= 2,248> t tabel= 1,975), dan tidak ada pengaruh signifikan secara parsial terhadap sindrom metabolik dengan kebugaran fisik kelompok usia lanjut (t= -0,029 < t tabel= 1,975). Berdasar atas hasil penelitian dapat disimpulkan hubungan = aktivitas fisik, keseimbangan energi, dan sindrom metabolik dengan kebugaran fisik guru besar Universitas Padjadjaran. Kata kunci: Kebugaran, keseimbangan energi, sindrom metabolik