1. PIKEPERCH MILT PRESERVATION ON SHORT AND MEDIUM PERIODS.
- Author
-
Ada, Telea, Grozea, A., Dunea, I. Banatean, Korbuly, B., and Dumitrescu, Gabi
- Subjects
FISH spermatozoa ,VERTEBRATES ,AQUATIC animals ,SPERMATOZOA ,GERM cells ,GAMETES - Abstract
The pikeperch (Sander lucioperca) is a Eurasian piscivoroas fish. It is breaded in numerous farms in Europe mainly for two of its characteristics: boneless meat and on the other hand for the predation of wild fish that penetrates fish ponds and become food competitors for farmed cyprinids. The aim of our paper was to establish if the pikeperch milt can be preserved for short or medium periods of time with viability satisfactory for fertilization. For the activity of preserving on short or medium term the pikeperch milt there was collected semen from males by catheter. The seminal material was transferred to sterile and dry flasks. The samples were introduced in refrigerator at 4°C. For preserving the milt three working variant were tested with 2 ml of semen/variant. 1. V
1 . The seminal material was transferred into sterile and dry flasks and maintained to refrigerator at 4°C. 2. V2 . The seminal material was transferred into sterile and dry flasks and maintained to refrigerator at 4°C. At 24 hours the air in the flask was fleshed for oxygenation. 3. V3 . The seminal material was transferred into sterile and dry flasks and maintained to refrigerator at 4°C. At 24 hours the semen was aired by bubbling air. The evaluation of the viability was performed by activation of the sperm with water and the observation of the motility at microscope with immersion objective 100X. Intermediary and final time were recorded: intermediary time (Ti) = the time when 50% of the sperm in the field was mobile; final time (Tf) -- the time when there were no mobile sperm in the microscope field. At 7 days from the recovery, for V1 there was an intermediary time (Ti) of 2'50" and a final time (Tf) of 4'3", for the V2 at 7 days from recovery there was recorded Ti = 50" and Tf = 1'5". There can be noticed that the best results were obtained in V1 , and the poorest results were obtained in V3 . The short and medium time preservation of the semen from pikeperch at 4° C can be performed with success for 7 days the motility for the sperm being satisfactory for fecundation (V1 Tf= 4'3"); [ABSTRACT FROM AUTHOR]- Published
- 2008