1. DNA Damage of Lung Cells from Immature Cadmium-Ingested Mice
- Author
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Xue-feng Yang, Hai-tang Zhang, Guo-ying Fan, Dong-yang Liu, Ya-ming Ge, Jin-qing Jiang, and Zi-liang Wang
- Subjects
Cadmium ,Comet assay ,DNA damage ,Immature mice ,Lung cell ,Animal culture ,SF1-1100 ,Veterinary medicine ,SF600-1100 - Abstract
The objective of this study was to investigate the effects of cadmium on DNA damage of lung cells in immature animals. Seventy-two immature mice were randomly divided into twelve cadmium-ingested groups including low dose (1/100 LD50, 1.87 mg/kg BW), middle dose (1/50 LD50, 3.74 mg/kg BW), high dose (1/25 LD50, 7.48 mg/kg BW) and control group, and exposed to cadmium chloride for 10, 20 and 30 days, respectively. Mice were sacrificed after cadmium exposure for different time, and lung cells were collected to investigate DNA damage by comet assay. The results showed that comet tailing ratio, tail length, comet length, tail moment, Olive tail moment and damaged grade of lung cells from immature mice increased along with increasing of cadmium exposure dose and time. In low dose group treated for 30 days, there was significance (P
- Published
- 2014