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The effectiveness of breeding is largely depends on the presence of the gene variability in the initial breeding material. The genetic variability assessment helps to evaluate the source material during a fundamentally new forms with economically valuable traits creating. In this regard, the assessment of the genetic polymorphism of the cereal grasses genotypes which are of interest both as a source of breeding material and as a variety candidate was carried out by DNA markers. Seventy-three loci were identified for the Festulolium and Lolium L. genotypes using the SCoT marker system, 69 of markers were polymorphic. This marker system revealed a high level of polymorphism in the studied genotypes of Festulolium and Lolium L. - 94.52%. For the genotypes of intergeneric hybrids of the Agropyron L. genus and their parent forms 90 loci were identified - 46 SCoT markers and 44 SRAP markers. From the total pool of markers 73 markers were polymorphic. On average, the level of polymorphism was 81.1%. For the genotypes of the Alopecurus L. genus interspecific hybrids and their parent forms 157 loci were identified - 52 for RAPD-PCR and 105 - for ISSR-PCR. Of the total pool of markers, 104 were polymorphic According to the results of DNA genotyping the genetic passports of cereal grasses were compiled. [ABSTRACT FROM AUTHOR]