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First larval stage (L1) of Oestrus ovis was recovered by flushing of the nasal cavity during rhinoscopy in an urban living dog. The dog was taken to the Small animal clinic after an acute onset of sneezing and bilateral nasal discharge. In Europe, there are sporadic reports of nasal myiasis in dogs caused by sheep bot flies, and the overall prevalence of O. ovis is high in Mediterranean countries. Because of its habitat expansion due to climate change, it should be considered as a differential diagnosis when an animal patient presents with signs of rhinitis in areas bordering the Mediterranean climate. This is the first report of a dog infested by sheep nasal bot fly in Slovenia. [ABSTRACT FROM AUTHOR]

Giardiosis is a parasitic disease prevalent worldwide that affects humans, domestic and wild animals and can be responsible for chronic diarrhoea. Diarrhoea started in the time of wintering at the stables of the Institute for the Health Care of Pigs. Two sows (Krsko polje breed), and four piglets aged two months (the Krsko polje breed) were affected. Rectal smears were sent for bacteriological examination. Faecal samples were examined by microscopy using the SAF method for concentrating protozoa and by direct immunofluorescence (DIF). No pathogenic bacteria were isolated at the first bacteriological examination but the second examination confirmed the presence of Campylobacter coli. Giardia sp. was detected by SAF and DIF at the first test. The pigs were treated with Monil®5% (Pliva d.d., Croatia) with 20 mg/kg albendazole, per os, once daily, for 3 days. After 3 days therapy the diarrhoea stopped and the pigs were clinically healthy and remained without diarrhoea for the next four months. Seven days, ten days and eleven days after the treatment all tested samples were negative by SAF and DIF. [ABSTRACT FROM AUTHOR]

A 3-month-old dog originating from a Slovenian animal shelter presented with acute bloody, soft, foamy and malodorous diarrhoea. The clinical examination, haematology and serum biochemistry were unremarkable. Ultrasonography of the abdomen showed prominent mesenteric lymph nodes and the presence of echogenic content within the small intestine. Light microscopy of a native smear and a wet mount darkfield microscopy examination of the faecal material showed motile trichomonad-like organisms with a particular circular motion. The flotation and SAF (Sodium acetate - acetic acid - formalin solution) method using light microscopy revealed eggs of nematode Toxocara canis and protozoan oocysts of Isospora spp. Trichomonad-like organisms were successfully isolated and cultivated in axenic culture. Light microscopy of Giemsa-stained trichomonads showed the presence of five flagella, and Pentatrichomonas hominis (P. hominis) was presumptively diagnosed. The diagnosis was confirmed by the Polymerase Chain Reaction (PCR) followed by DNA sequencing and the Scanning Electron Microscopy (SEM) of cultured trichomonad isolates. The PCR and sequencing results confirmed a 99% homology of the P. hominis isolates with isolates from other studies, originating both from humans and animals, which suggests that P. hominis could have zoonotic potential and have been transmitted from animals to people via the per-oral route. This is also the first report on P. hominis involvement in clinical diseases in dogs in Slovenia. [ABSTRACT FROM AUTHOR]