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Novianty R, Antika B, Saryono, Awaluddin A, Pratiwi NW, Juliantari E. 2022. Naphthalene degradation by Pseudomonas sp. LBKURCC419 strain with addition of glucose as cosubstrate. Biodiversitas 23: 5654-5661. Naphthalene is one of the pollutants that cause carcinogens. The biodegradation of carcinogenic naphthalene toxic compounds needs to be done to control pollution. Pseudomonas is one of several bacterial species that can degrading naphthalene. This study aimed to test the effectiveness of Pseudomonas sp. isolated from the land area of ??the Joint Operations Agency of PT. Siak Bumi Pusako-Pertamina Hulu (BOB BSP-PHE) in degrading naphthalene with glucose as a cosubstrate. The potency of three isolates of Pseudomonas sp. LBKURCC148, LBKURCC149, and LBKURCC150 was tested in Minimal Media (MM) containing 0.2 mM naphthalene and incubated for seven days. The Optical Density (OD), pH, decreased concentration of naphthalene and degradation ability parameters were analyzed using UV-Vis spectrophotometer. The results showed that Pseudomonas sp. LBKURCC149 was able to degrade naphthalene by 13.95%. Naphthalene analysis using HPLC resulted a decrease in the peak area of ??the sample as compared to the control. Based on this findings, Pseudomonas sp. LBKURCC149 could be recommended to use to clean up naphthalene pollution.

Novianty R, Saryono, Awaluddin A, Pratiwi NW, Hidayah A, Juliantari E. 2021. The diversity of fungi consortium isolated from polluted soil for degrading petroleum hydrocarbon. Biodiversitas 22: 5077-5084. One of the major problems in the petroleum industry nowadays is crude oil spills. Riau Province, Sumatra is one of the largest oil producers in Indonesia. Accidental releases of petroleum products are of particular concern to the environment. The process of drilling and refining petroleum generates a large amount of oil sludge. One of the effective technologies used in the waste degradation process is bioremediation using certain microorganisms. The prime objective of the current research was to evaluate the efficiency of fungi consortiums in crude oil degradation in Bumi Siak Pusako-Pertamina Hulu, Indonesia. There are three potential fungi isolates as petroleum hydrocarbon degradation agents with four consortium variations. The parameter values of Optical Density (OD), pH, and diluted CO2 were measured on 0, 4, 8, and 16 days. To evaluate the fungal biodegradation activity using Gas Chromatography-Mass Spectrometry (GC-MS). The result showed that consortium II (KF II) has the highest potential to degrade petroleum hydrocarbon (50.61%). The visual GC-MS examination confirmed a decrease in the peak area for eight hydrocarbon compounds, indicating the efficiency of the fungi in the oil decomposition and dismantling of hydrocarbons. Our findings may provide new information on native fungal resources from chronically contaminated terrestrial environments, and will be useful for petroleum-contaminated bioremediation and other industrial applications.

Permana I, Awaluddin A, Saryono. 2019. Methylene blue decolorization fungi from crude oil-contaminated soils. Biodiversitas 20: 2693-2697. One of the world problems is the treatment to remove dyes in textile wastewater. Nowadays, the most effective and compatible method to the environment is decolorization by microorganisms. Therefore, the objective of this study is to find out the best fungi to decolorize synthetic dye methylene blue (MB). Total eight fungi that isolated from crude oil-contaminated soil were analyzed to study the effect of various parameters such as pH, the concentration of dyes, contact times and agitation on their rate of reaction. The initial screening showed that Penicillium sp. FTM7 had greater potential in biodegradation the MB. The optimum decolorization of MB by Penicillium sp. was found after 8 days incubation with agitation 150 rpm and concentration of MB is 40 ppm and unadjusted pH (95.45%). The decolorization of MB was found to follow first-order reactions.