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Whole cell Leptospirosis vaccines have been developed and used from the 20's of last century up today, most of them are not adjuvated and do not confer a long-lasting immunity. These vaccines are mainly against different serogroups of Leptospira interrogans contained in the preparation. Though several attempts to obtain a vaccinal formulation more effective, purer, with wider spectrum and longer protection than the bacterines of inactivated whole cells have been made no vaccine with these characteristics have been registered yet. This paper deals with the obtainment of outer membrane antigens from a Cuban autochthonous strain (Strain 87, L. interrogans serovar Canicola), by a modification of the technology for the production of outer membrane vesicles, patented by researchers from Finlay institute These antigens with nanoproteoliposomic structure were formulated/adjuvated by different strategies, obtaining five preparations with cochlear structures which are nanoparticles of approximately 100 to 150 nm of length and from 15 to 30 nm of diameter. Two doses of the immunogens are inoculated in the biomodel Mesocrisetus aureatus, with an interval of six weeks. The challenge was carried out with 100.000 DL50. Results showed that the new vaccinal formulations confer protection against the homologous challenge and were able to eliminate the carrier status. This, together with the robutness of the preparation method, the higher level of purity, compared to the bacterines, and the no need of aluminium hydroxide make the formulations an alternative of interest for continuing their development. [ABSTRACT FROM AUTHOR]

Combined vaccines have a higher level of acceptance in the population because fewer immunisations are required for protection against infectious diseases. Nevertheless, new challenges have appeared due to the report of some interference among the antigens included in such vaccines. The aim of this paper was to evaluate whole-cell Pertussis (wP) interference in potency tests with other antigens like tetanus, Haemophilus influenzae type b (Hib) and hepatitis B. For that purpose, we evaluated combined vaccines and compared them with the respective monovalent vaccines. Likewise, we prepared experimental formulations with different antigen combinations to evaluate the extent and nature of the interference caused by wP. We obtained a significant increase of the tetanus and Hib biological activities when both antigens are combined with wP, although it depended on the animal model and the experimental design used. On the other hand, wP decreased both the in vivo and in vitro potency of HB in a non-significant way. [ABSTRACT FROM AUTHOR]

Atherosclerosis, is the most common process subordinated to cardiovascular morbidity and mortality. The isquemic cardiopatic as consequence of the atherosclerotic process, has in Cuba the first place among non violent dysfunctions. The participation of the immune system in the atherosclerosis has studied in the last years. In this paper we proposed to obtain an passive experimental model of atherosclerosis induced by immune complex in BALB/CBJ Hepatitis B surface antigen (HbsAg) transgenic (Tg) mice. We obtain antibodies against the HBsAg (anti-HBs) developing ascitis rich in these antibodies in BALB/CBJ isogenic mice. We employed two groups of BALB/CBJ female mice transgenic to HBsAg of six weeks of age under a regimen of water and food at libitum. We quantified the concentration of HBsAg in the animals by means of an immunoenzimatic method. Later we performance seven inoculations by the caudal vein with an interval of five days between them, utilizing the anti-HBs that was obtained previously in the study group with a dose that duplicated the HBsAg concentration of each animal, in the control group we used ascites free of these antibodies with the same procedure. A week after these immunizations we took blood samples of the animals to determine the circulates immune complex in plasma. The animals were sacrificed by cervical dislocation and the presence of atherosclerotic lesions in the aorta was observed by macroscopic and microscopic manner. In the study group we observed the presence of atheroma plaques but not in the controls in both groups we observed the fatty streaks. The circulates immune complex were detected in both groups. With these results we concluded that we obtained an atherosclerosis model in BALB/CBJ isogenic mice by passive way of immune complex formation. [ABSTRACT FROM AUTHOR]

Atherosclerosis, is the most common process subordinated to cardiovascular morbidity and mortality. The isquemic cardiopatic as consequence of the atherosclerotic process has in Cuba the first place among non violent dysfunctions. The participation of the immune system in the atherosclerosis has studied in the last years. In this paper we proposed to obtain an active experimental model of atherosclerosis induced by immune complex in BALB/CBJ isogenic mice. We employed male mice of six weeks of age under a regimen of water and food at libitum. These animals were divided in two groups, one of them received a scheme of 0-1-4 weeks intraperitoneal via with the cuban anti Hepatitis B vaccine Heberbiovac HB. The other group was the control and was immunized the same way employing the excipient of the vaccine without the Hepatitis B surface antigen (HBsAg). When the scheme finalized we took blood samples of the animals and we quantified the antibodies against the HBsAg (anti-HBs) by means of an immunoenzimatic method. Later we performance seven immunizations by the caudal vein with an interval of five days between them, utilizing as immunogen recombinant HBsAg in the study group with a dose that duplicated the anti-HBs concentration of each animal, in the control group we used saline solution with the same procedure. A week after these immunizations we took blood samples of the animals to determine the circulates immune complex in plasma. During the experiment the animals of the study group inhaled cigarette smoke. The animals were sacrificed by cervical dislocation and the presence of atherosclerotic lesions in the aorta was observed by macroscopic and microscopic manner. In the study group we observed the presence of atheroma plaques but not in the controls in both groups we observed the fatty streaks. The circulates immune complex were detected in none groups. With these results we concluded that we obtained an atherosclerosis model in BALB/CBJ isogenic mice by active way of immune complex formation. [ABSTRACT FROM AUTHOR]

Vaccination against cholera continues being the main way to eradicate the disease. Development of oral inactivates vaccines has recaptured validity in the last years. In this paper, Vibrio cholerae strain C7258 was inactivated by heat, and the presence of antigen like LPS, MSHA and TCP was demonstrated by Dot Blot technique. Inactivated strain was inoculated at 0 and 14 days by intraduodenal route to 4 white New Zeland rabbits. Blood samples at day 0 and 7, 14, 21, 28, 35 and 42 post inoculation was taken in order to determine vibriocidal antibodies titer and IgG anti LPS antibodies by ELISA. Evaluated antigens in the suspensions of inactivated cells were present. By the other hand, high vibriocidal titers and IgG anti LPS were obtained. These ones were similar to induced response, reported to live V. cholerae attenuated strain 638, oral vaccine candidate. [ABSTRACT FROM AUTHOR]

The control of the tuberculosis is based in the early diagnose, treatment of the cases and vaccination with BCG. However, the methods of diagnose in use possess a relatively low sensibility, due to this development of methods of quick diagnose, with a high sensibility it constitutes a priority at the present time. Between the methods more attractiveness for these goals are the inmunoenzimatics methods of detection of antibodies, directed against proteins of Micobacterium tuberculosis. In this paper it was study the answer of antibodies serics IgG directed against the protein of 38 kd of Micobacterium tuberculosis in a group of healthy individuals of different groups vaccinated with BCG, with the purpose of determining the pattern of the normal population's answer in front of this antigen and the possible interference of antibodies of crossed reaction induced by the vaccination, this will be useful for the development of serologic diagnose methods based on the use of this protein. [ABSTRACT FROM AUTHOR]