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Plantlets of sweet potato ( Ipomoea batatas L. ) were cultured in vitro under three different ambient conditions including a standard culture room - PS, a culture room inside a glasshouse with natural light but controlled temperature - TH, and a standard glasshouse with natural light (natural fluctuations of temperature) - NP. Plantlets from three treatments were compared in terms of pathogen rate, growth, survival plant at the end of the in vitro stage and at the ex vitro acclimatization. This result showed that, after 28 days of culture, sweet potato plants were cultured in vitro TH conditions have reduced entirely due to susceptibility to fungal disease causing outside air. After 14 days of ex vitro acclimatization, plants originally grow in vitro under the TH condition had ability to adapt about field survival and growth rates better than the other two treatments.