1. Cholecalciferol Supplementation Did Not Change Interleukin-7 and B cell-Activating Factor Levels and CD95 Expression in B lymphocytes in Patients on Dialysis: A Randomized Pilot-Controlled Trial.
- Author
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de Carvalho JTG, Schneider M, Cuppari L, Grabulosa CC, Cendoroglo M, and Dalboni MA
- Subjects
- Adult, Aged, B-Cell Activating Factor metabolism, B-Lymphocytes metabolism, Dietary Supplements, Double-Blind Method, Female, Humans, Interleukin-7 metabolism, Kidney Failure, Chronic blood, Kidney Failure, Chronic complications, Lymphopenia blood, Lymphopenia complications, Male, Middle Aged, Pilot Projects, Uremia blood, Uremia metabolism, Vitamin D blood, Vitamin D Deficiency blood, Vitamin D Deficiency complications, Vitamins administration & dosage, fas Receptor metabolism, B-Lymphocytes drug effects, Cholecalciferol administration & dosage, Kidney Failure, Chronic therapy, Renal Dialysis, Vitamin D Deficiency drug therapy
- Abstract
Background: End-stage renal disease results in B cell lymphopenia and low levels of vitamin D. Since the link between vitamin D deficiency and B lymphocytes dysfunction are not clear in patients with end-stage renal disease, we suggest that vitamin D adequacy and factors related to the homeostasis of these cells should be investigated. B lymphocytes homeostasis is a process mainly regulated signals of grown and death as interleukin (IL)-7, B cell-activating factor (BAFF)/BAFF-receptor and CD95 expression., Objective: As vitamin D serum levels were reduced in patients with end stage renal disease and it is associated with human B homeostasis, we evaluated the effect of cholecalciferol supplementation on dialysis., Design: Randomized, double blind clinical trial in dialysis patients with 25OH Vitamin D deficiency for a period of 12 weeks., Main Outcome Measure: In a pilot study, we investigated the effect of cholecalciferol supplementation (100,000 UI once per week or placebo. In vitro, peripheral blood mononuclear cells isolated by Ficoll-Hypaque centrifugation from 12 healthy volunteers were incubated with healthy or uremic serum in the presence or absence of 25 (OH)DC with 5% CO., Results: There was an increase in the serum 25(OH)D level in the cholecalciferol group. No differences were found in BAFF and IL7 levels and CD95 and BAFF-R expression in B lymphocytes from patients on dialysis after cholecalciferol supplementation. Uremic serum induced an increase in the IL-7, BAFF, BAFF-R and CD95 expression compared with the control. However, we observed no effect of incubation of 25(OH)D3 and 1,25(OH)2D3 on the expression of IL-7, BAFF, BAFF-R and CD95 when incubated in the presence of normal or uremic serum., Conclusion: Our results suggest that vitamin D is not involved in mechanisms of regulation of differentiation and survival in B lymphocytes. In conclusion, further studies are needed to explore the effects of vitamin D on B lymphocytes to better evaluate the possible impact of vitamin D on humoral response in the CKD population., (Published by Elsevier Inc.)
- Published
- 2019
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