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For the determination of creatine kinase isoenzyme catalytic concentrations in serum two methods based on immunological reactions are presented: One method uses inhibiting antibodies, which selectively block the activity of creatine kinase M subunits ("Inhibition Test"). This test is used for routine measurements of creatine kinase MB catalytic concentration; Another method uses precipitating antibodies, which allows a quantitative differentiation of creatine kinase isoenzymes MM, MB and BB ("Precipitation Test"). This test is used as a control for the Inhibition Test for the possible presence of creatine kinase BB activities in doubtful cases. Procedures, specificity, correlation and application of these methods are discussed.

We have established several mouse hybridoma cell lines which secrete monoclonal antibodies with creatine kinase M subunit inhibiting capacity. Some of these antibodies are useful tools for the determination of the creatine kinase-B subunit activity (CK-MB test). The monoclonal mouse anti-bodies and the conventional polyclonal goat antibodies were compared.