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Auf Basis von Real-Time Location Systemen (RTLS) können in Echtzeit Produktionsdaten digitalisiert, analysiert und dargestellt werden. Diese Daten und Informationen können dann im Rahmen von Lean Management Methoden verwendet werden, um Optimierungspotenziale von Produktionsprozessen aufzudecken und zu realisieren. Dieser Beitrag zeigt den Einsatz von RTLS mit einer Microsoft Excel-basierten Anwendung zur Prozessoptimierung in einem manuellen Produktionsumfeld und die damit verbundenen Vorteile.

Alkylglycerol monooxygenase (AGMO; EC 1.14.16.5) is the only enzyme known to cleave the O-alkyl ether bond of alkylglycerols in humans. It is an integral membrane protein with nine predicted transmembrane domains. We attempted to express and purify full-length and truncated forms of AGMO in Escherichia coli. Full-length AGMO could not be expressed in three different E. coli expression strains, three different expression vectors and several induction systems. We succeeded, however, in expression of three N-terminally strep-tagged truncated forms, named active sites 1, 2 and 3, with 205, 134 and 61 amino acids, respectively. Active site 1 fragment, containing two predicted transmembrane regions, a membrane associated region and all known amino acid residues important for catalytic activity, was not fully soluble even in 8 M urea. Active site 2 containing only one predicted membrane associated domain required 8 M urea for solubilisation and eluted in gel filtration in 1 M urea as a trimer. Active site 3 with no hydrophobic domain eluted in gel filtration in 1 M urea as monomer and dimer. These results show that even truncated forms of AGMO are barely soluble when expressed in E. coli and show a high tendency for aggregation.