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We present a genome assembly from an individual male acalyptrate fly Dryomyza anilis (Arthropoda; Insecta; Diptera; Dryomyzidae). The genome sequence has a total length of 656.60 megabases. Most of the assembly is scaffolded into 7 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 16.48 kilobases in length.

We present a genome assembly from an individual female Palloptera scutellata (the flutter-wing fly; Arthropoda; Insecta; Diptera; Pallopteridae). The genome sequence is 415.6 megabases in span. Most of the assembly is scaffolded into 5 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 15.93 kilobases in length.

We present a genome assembly from an individual female Syritta pipiens (the Thick-legged Hoverfly; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 318.5 megabases in span. Most of the assembly is scaffolded into 5 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 15.76 kilobases in length. Gene annotation of this assembly on Ensembl identified 18,405 protein coding genes.

We present a genome assembly from an individual male Cheilosia variabilis (the Figwort Cheilosia; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 414.7 megabases in span. Most of the assembly is scaffolded into 7 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 16.77 kilobases in length.

We present a genome assembly from an individual female Myopa tessellatipennis (Arthropoda; Insecta; Diptera; Conopidae). The genome sequence is 249.3 megabases in span. Most of the assembly is scaffolded into four chromosomal pseudomolecules, including the assembled X sex chromosome. The mitochondrial genome has also been assembled and is 18.3 kilobases in length.