1. Heat inactivation of the <scp>M</scp> iddle <scp>E</scp> ast respiratory syndrome coronavirus
- Author
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India Leclercq, Ana Maria Burguiere, Jean Claude Manuguerra, Christophe Batéjat, Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats (CIBU), Institut Pasteur [Paris] (IP), The research leading to these results has received funding from the European Union's Seventh Framework Programme for research, technological development and demonstration under Grant Agreement N° 278433-PREDEMICS. We are also grateful to BNP-Paribas and its foundation (http://www.mecenat.bnpparibas.com/) for their financial support., We also thank Gilberte Coralie, Frederic Fichenick and Claudine Rousseaux for helping., and European Project: 278433,EC:FP7:HEALTH,FP7-HEALTH-2011-two-stage,PREDEMICS(2011)
- Subjects
Pulmonary and Respiratory Medicine ,Hot Temperature ,Epidemiology ,Middle East respiratory syndrome coronavirus ,[SDV]Life Sciences [q-bio] ,viruses ,Biology ,medicine.disease_cause ,Microbiology ,MERS-CoV ,03 medical and health sciences ,Biosafety ,medicine ,Humans ,inactivation ,030304 developmental biology ,Infectivity ,0303 health sciences ,030306 microbiology ,Public Health, Environmental and Occupational Health ,virus diseases ,Short Articles ,Heat ,Virology ,Disinfection ,Heat inactivation ,Titer ,Infectious Diseases ,Middle East Respiratory Syndrome Coronavirus ,Vero cell ,Cell culture supernatant ,Coronavirus Infections - Abstract
International audience; The culture supernatants of the emerging Middle East respiratory syndrome coronavirus (MERS-CoV) were submitted to three temperatures over time and tested for infectivity by TCID50 method on Vero E6 cells. At 56°C, almost 25 minutes were necessary to reduce the initial titre by 4 log10 . Increasing temperature to 65°C had a strong negative effect on viral infectivity as virucidy dropped significantly to 1 minute. On the contrary, no significant decrease in titre was observed after 2 hours at 25°C. These data might be useful in establishing biosafety measures in laboratories against MERS-CoV.
- Published
- 2014
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