1. Mechanism of Proton Relaxation for Enzyme‐Manipulated, Multicomponent Gold–Magnetic Nanoparticle Chains
- Author
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Aneesh K. Ramaswamy, Michael Kellogg, Andrew Wager, Katherine Hudson, Tyler A. Smith, Ashley N. Vacchiano, Richard L. Gieseck, Kimberly E. Raver, Albena Ivanisevic, and Hamsa Jaganathan
- Subjects
Magnetic Resonance Spectroscopy ,Chemistry ,Relaxation (NMR) ,Spin–lattice relaxation ,Oxide ,Analytical chemistry ,Contrast Media ,Nanoparticle ,DNA ,DNA Restriction Enzymes ,Nuclear magnetic resonance spectroscopy ,Atomic and Molecular Physics, and Optics ,Spin–spin relaxation ,Magnetics ,chemistry.chemical_compound ,Crystallography ,Bacterial Proteins ,Proton NMR ,Humans ,Nanoparticles ,Gold ,Protons ,Physical and Theoretical Chemistry ,Superparamagnetism - Abstract
Longitudinal and transverse relaxation times of multicomponent nanoparticle (NP) chains are investigated for their potential use as multifunctional imaging agents in magnetic resonance imaging (MRI). Gold NPs (ca. 5 nm) are arranged linearly along double-stranded DNA, creating gold NP chains. After cutting gold NP chains with restriction enzymes (EcoRI or BamHI), multicomponent NP chains are formed through a ligation reaction with enzyme-cut, superparamagnetic NP chains. We evaluate the changes in relaxation times for different constructs of gold-iron oxide NP chains and gold-cobalt iron oxide NP chains using 300 MHz (1)H NMR. In addition, the mechanism of proton relaxation for multicomponent NP chains is examined. The results indicate that relaxation times are dependent on the one-dimensional structure and the amount of superparamagnetic NP chains present in the multicomponent constructs. Multicomponent NP chains arranged on double-stranded DNA provide a feasible method for fabrication of multifunctional imaging agents that improve relaxation times effectively for MRI applications.
- Published
- 2010
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