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Fumonisin B1 (FB1 ) is a mycotoxin produced by several Fusarium species and is a very common contaminant of maize-based food and feed throughout the world. The selection and use of FB1 -degrading microorganisms appears as a promising alternative to cope with the problem of toxicity towards humans and livestock. High moisture maize grain silage, which is based on natural maize fermentation, could be an interesting reservoir of such microorganisms.; Results: Using an in vitro simulated silage model with FB1 naturally contaminated grains, we demonstrated a significant raw decrease in FB1 during ensiling process ascribed to biodegradation mechanisms. A panel of 98 bacteria and yeasts were isolated from this matrix and selected for their ability to use FB1 as the sole source of C and N. For nine of them, the ability to degrade FB1 in vitro was evidenced. Notably, two bacteria identified as Lactobacillus sp. were highlighted for their efficient FB1 -degrading capacity and production of hydrolysed FB1 as intermediate degradation metabolite.; Conclusion: Fermentation of high moisture maize grain contaminated with FB1 leads to a significant reduction of the toxin and allows the isolation of FB1 -degrading microorganisms that could further be used as FB1 decontaminating agents. © 2016 Society of Chemical Industry.; © 2016 Society of Chemical Industry.