1. Microarray profile of micro-ribonucleic acid in tumor tissue from cervical squamous cell carcinoma without human papillomavirus
- Author
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Yuanshuai Huang, Yi-Bing Yin, Yanliang Zhang, Yong Dai, Min Tang, Liguo Ma, and Cheng-Xiao Hu
- Subjects
Adult ,Pathology ,medicine.medical_specialty ,Microarray ,Gene Expression Profiling ,Cellular differentiation ,Cell ,Uterine Cervical Neoplasms ,Obstetrics and Gynecology ,Middle Aged ,Biology ,Blotting, Northern ,Non-coding RNA ,Pathogenesis ,MicroRNAs ,medicine.anatomical_structure ,microRNA ,Carcinoma, Squamous Cell ,Gene chip analysis ,medicine ,Humans ,Female ,Northern blot ,Biomarkers ,Oligonucleotide Array Sequence Analysis - Abstract
Aims: Micro-ribonucleic acid (miRNA) are noncoding RNA molecules of 21 to 24 nt that regulate the expression of target genes in a post-transcriptional manner. Evidence indicates that miRNA play essential roles in embryogenesis, cell differentiation and pathogenesis of human diseases. This study describes a comparison between the microRNA profile of human-papillomavirus-negative cervical squamous cell carcinoma patients and controls, in order to develop further understanding of the pathogenesis of cervical squamous cell carcinomas. Methods: MiRNA were isolated from tumor tissues of five human-papillomavirus-negative cervical squamous cell carcinoma patients and five healthy controls in order to perform miRNA microarray chip analysis. The chip results were then confirmed by northern blot analysis. Results: A total of 27 miRNA differentially expressed between the squamous cell carcinoma patients and the healthy controls were identified. Conclusion: This work indicates that these miRNA may be potential diagnosis biomarkers and probable factors involved in the pathogenesis of cervical squamous cell carcinomas.
- Published
- 2009
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