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Your search keyword '"Cropek, Donald M."' showing total 13 results
Start Over Author "Cropek, Donald M." Publisher wiley
13 results on '"Cropek, Donald M."'

1. A 3D Interconnected Microchannel Network Formed in Gelatin by Sacrificial Shellac Microfibers

Author

Koch Institute for Integrative Cancer Research at MIT, Bellan, Leon M., Pearsall, Matthew, Cropek, Donald M., Langer, Robert S, Koch Institute for Integrative Cancer Research at MIT, Bellan, Leon M., Pearsall, Matthew, Cropek, Donald M., and Langer, Robert S

Abstract

3D microfluidic networks are fabricated in a gelatin hydrogel using sacrificial melt-spun microfibers made from a material with pH-dependent solubility. The fibers, after being embedded within the gel, can be removed by changing the gel pH to induce dissolution. This process is performed in an entirely aqueous environment, avoiding extreme temperatures, low pressures, and toxic organic solvents. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Published
2022

2. A 3D Interconnected Microchannel Network Formed in Gelatin by Sacrificial Shellac Microfibers

Author

Bellan, Leon M, Pearsall, Matthew, Cropek, Donald M, Langer, Robert, Bellan, Leon M, Pearsall, Matthew, Cropek, Donald M, and Langer, Robert

Abstract

3D microfluidic networks are fabricated in a gelatin hydrogel using sacrificial melt-spun microfibers made from a material with pH-dependent solubility. The fibers, after being embedded within the gel, can be removed by changing the gel pH to induce dissolution. This process is performed in an entirely aqueous environment, avoiding extreme temperatures, low pressures, and toxic organic solvents. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Published
2021

3. Molecular and Topographical Organization: Influence on Cicada Wing Wettability and Bactericidal Properties

Author

Román‐Kustas, Jessica, primary, Hoffman, Jacob B., additional, Reed, Julian H., additional, Gonsalves, Andrew E., additional, Oh, Junho, additional, Li, Longnan, additional, Hong, Sungmin, additional, Jo, Kyoo D., additional, Dana, Catherine E., additional, Miljkovic, Nenad, additional, Cropek, Donald M., additional, and Alleyne, Marianne, additional

Published
2020
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13. High affinity peptides for the recognition of the heart disease biomarker troponin I identified using phage display.

Author

Park JP, Cropek DM, and Banta S

Subjects
Amino Acid Sequence, Animals, Culture Media metabolism, Enzyme-Linked Immunosorbent Assay, Humans, Peptides analysis, Protein Binding, Rats, Sequence Analysis, Protein, Serum metabolism, Troponin I metabolism, Heart Diseases diagnosis, Peptide Library, Peptides metabolism, Troponin I analysis
Abstract

Troponin I is a specific and sensitive clinical biomarker for myocardial injury. In this study we have used polyvalent phage display to isolate unique linear peptide motifs which recognize both the human and rat homologs of troponin I. The peptide specific for human troponin I has a sequence of FYSHSFHENWPS and the peptide specific for the rat troponin I has a sequence of FHSSWPVNGSTI. Enzyme-linked immunosorbent assays (ELISAs) were used to evaluate the binding interactions, and the two phage-displayed peptides exhibited some cross-reactivity, but they were both more specific for the troponin I homolog they were selected against. The binding affinities of the phage-displayed peptides were decreased by the presence of complex tissue culture media (MEM), and the addition of 10% calf serum further interfered with the binding of the target proteins. Kinetic indirect phage ELISAs revealed that both troponin I binding peptides were found to have nanomolar affinities for the troponin proteins while attached to the phage particles. To our knowledge, this is the first example of isolation and characterization of troponin I binders using phage display technology. These new peptides may have potential utility in the development of new clinical assays for cardiac injury as well as in monitoring of cardiac cells grown in culture., ((c) 2009 Wiley Periodicals, Inc.)

Published
2010
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