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Abstract The organophosphate (OP)‐hydrolyzing enzyme phosphotriesterase (PTE, variant L7ep‐3a) immobilized within a partially oxidized mesoporous silicon nanoparticle cage is synthesized and the catalytic performance of the enzyme@nanoparticle construct for hydrolysis of a simulant, dimethyl p‐nitrophenyl phosphate (DMNP), and the live nerve agent VX is benchmarked against the free enzyme. In a neutral aqueous buffer, the optimized construct shows a ≈2‐fold increase in the rate of DMNP turnover relative to the free enzyme. Enzyme@nanoparticles with more hydrophobic surface chemistry in the interior of the pores show lower catalytic activity, suggesting the importance of hydration of the pore interior on performance. The enzyme@nanoparticle construct is readily separated from the neutralized agent; the nanoparticle is found to retain DMNP hydrolysis activity through seven decontamination/recovery cycles. The nanoparticle cage stabilizes the enzyme against thermal denaturing and enzymatic (trypsin) degradation conditions relative to free enzyme. When incorporated into a topical gel formulation, the PTE‐loaded nanoparticles show high activity toward the nerve agent VX in an ex vivo rabbit skin model. In vitro acetylcholinesterase (AChE) assays in human blood show that the enzyme@nanoparticle construct decontaminates VX, preserving the biological function of AChE when exposed to an otherwise incapacitating dose.

Abstract Hunting has important social, cultural, economic, and conservation benefits. Information on hunters’ motivations and expectations for a hunting experience can help support decision‐making by state and federal wildlife managers. We surveyed deer (Odocoileus spp.) and elk (Cervus canadensis) hunters participating in controlled hunts in eastern Oregon to describe hunter characteristics and experiences, including the importance of hunting motivational factors and specific setting attributes (2017–2019; n = 501). We compared differences between hunters’ ratings of importance and satisfaction and identified factors of particular importance for hunters with different characteristics. Survey response rates were high, ranging from 97% in 2017 to 81% in 2019. We found that the intangible benefits of hunting were most important to hunters, with enjoyment and spending time with friends and family emerging as the top‐ranked hunting motivations by importance. Motivations differed by gender, age and hunting avidity. Women rated getting meat for themselves and family higher than did men ( x ¯ = 4.67 vs. 4.26). Frequent hunters ranked many experiential elements of a hunt higher in importance than less frequent hunters (e.g., Seeing animals in the wild, x ¯ = 4.52 vs. 4.19; Fun and enjoyment, x ¯ = 4.50 vs. 4.21; Thrill of the chase, x ¯ = 4.34 vs. 3.97). Older hunters considered learning experiences less important than younger hunters (New experience, developing new skills, x ¯ = 2.72 vs. 3.52; Gaining knowledge about the outdoors, x ¯ = 3.14 vs. 3.83). We also found that the attributes of the specific hunt setting were less important than the broad factors that motivate hunting participation. Our results provide context for hunting retention and reactivation efforts and can be used to identify opportunities to recruit new hunters whose diversity reflects that of background populations in the U.S.

Abstract Background This study aimed to determine how patients trade‐off the benefits and risks of two different types of procedures used to treat heart valve disease (HVD). It also aimed to determine patients' preferences for HVD treatments (predicted uptake) and the relative importance of each treatment attribute. Methods A discrete choice experiment (DCE) was conducted in Australia and Japan with patients who required a heart valve procedure. Patients were stratified into three categories: no prior procedure experience, minimally invasive procedure experience and invasive procedure experience. DCE attributes included risk of mortality; risk of stroke; needing dialysis; needing a new pacemaker; valve durability; independence 1 month after surgery; and out‐of‐pocket expenses. Participants chose between two hypothetical labelled approaches to therapy (‘invasive procedure’ and ‘minimally invasive procedure’), with a separate opt‐out included. A mixed multinomial logit model was used to analyse preferences. Results The DCE was completed by 143 Australian and 206 Japanese patients. Both populations demonstrated an overall preference for the minimally invasive procedure over the invasive procedure. All attributes tested significantly predicted choice and were important to patient decision‐making. However, patients' choices were most influenced by the durability of the valve and the likelihood of independence postprocedure, irrespective of their prior procedure experience. Differences in preference were observed between Australian and Japanese patients; valve durability was the most important attribute among Australian patients, while Japanese patients emphasised regaining independence postsurgery. Risk of mortality was less important relative to other key attributes in Japan; however, it remained significant to the model. Conclusions HVD patients prefer a minimally invasive procedure over an invasive procedure, irrespective of prior treatment experience. Key attributes contributing to treatment preferences are valve durability and faster recovery. These results can be used to help inform healthcare decision‐makers about what features of heart valve procedures patients value most. Patient and Public Contribution People with lived experience of HVD were included in multiple stages of the design phase of this research. First, patients and doctors were consulted by taking part in qualitative interviews. The qualitative interviews helped inform which treatment attributes to include in the DCE based on what was important to those with lived experience and those who help make treatment decisions on behalf of patients. Qualitative interview participants also assisted with the framing of questions in the online survey to ensure the terminology was patient‐friendly and relevant to those with lived experience. Following qualitative interviews, the DCE attribute list was agreed on in expert consultation with a steering committee, which included patient representatives and treating physicians (interventional cardiologists, cardiothoracic surgeons). The survey was also pilot tested with a small sample of patients and minor adjustments were made to the wording to ensure it was appropriate and meaningful to those with lived experience of HVD.

Background Prehospital activation of the cardiac catheter laboratory is associated with significant improvements in ST‐segment–elevation myocardial infarction (STEMI) performance measures. However, there are equivocal data, particularly within Australia, regarding its influence on mortality. We assessed the association of prehospital activation on performance measures and mortality in patients with STEMI treated with primary percutaneous coronary intervention from the Queensland Cardiac Outcomes Registry (QCOR). Methods and Results Consecutive ambulance‐transported patients with STEMI treated with primary percutaneous coronary intervention were analyzed from January 1, 2017 to December 31, 2020 from the QCOR. The total and direct effects of prehospital activation on the primary outcomes (30‐day and 1‐year cardiovascular mortality) were estimated using logistic regression analyses. Secondary outcomes were STEMI performance measures. Among 2498 patients (mean age: 62.2±12.4 years; 79.2% male), 73% underwent prehospital activation. Median door‐to‐balloon time (34 minutes [26–46] versus 86 minutes [68–113]; P

Core Ideas Further research is required to evaluate and develop management practices that may sequester C in turfgrass soils. Hidden C costs, which are energy‐based inputs from turf maintenance, should be factored into soil C sequestration calculations. A higher‐input management regime in turf will not increase net C sequestration compared with a low management input regime. Zoysiagrass golf course fairway turf had an average gross C sequestration rate of 1.01 Mg C ha–1 yr–1. Carbon dioxide (CO2) is an important greenhouse gas (GHG) implicated in climate change. Turfgrass covers an estimated 12.8 to 20 million ha in the United States and has the capacity to sequester or emit significant amounts of CO2 from/into the atmosphere. Our objective was to evaluate irrigation and N fertilization management practices that may increase sequestration of atmospheric CO2 in turf soils. The rate of change in soil organic carbon (SOC) at 0 to 30 cm was investigated under two management regimes in ‘Meyer’ zoysiagrass (Zoysia japonica Steud.). A high management input (HMI) (urea + medium irrigation) and low management input regime (LMI) (unfertilized [no N fertilizer] + low irrigation) were implemented. Hidden carbon costs (HCC) of maintenance practices and nitrous oxide emissions (another GHG) were estimated to account for energy expended in Mg of carbon equivalents (CE) ha–1 yr–1. Prior to subtracting HCC, average gross C sequestration rates were not statistically different at 1.046 and 0.976 Mg C ha–1 yr–1 in HMI and LMI, respectively. Once total estimated HCC was included, the average net sequestration rate was 0.412 and 0.616 Mg C ha–1 yr–1 in HMI and LMI, respectively, with no statistical differences. Results indicate that under the conditions of this study, a higher‐input management regime will not increase net C sequestration compared with a low management input regime. Further research is required over additional turfgrass species and management regimes to develop management practices that increase C sequestration.

Core Ideas Canopy temperature via measurements in turfgrass via drones have not been assessed. Rises in canopy temperature were detected before visible drought symptoms. This early detection was similar to top spectral parameters on companion flights. Canopy temperature was closely correlated with spectral data. Recent advances in aerial platforms and thermal imaging provide opportunities to improve water management in turfgrass, but research on this topic is limited. Our objectives were to: (i) evaluate the ability of canopy temperature (Tc) imaging from small unmanned aircraft systems (sUAS) to detect drought stress early in turfgrass; (ii) compare early drought‐stress detection ability of Tc measurements with that of sUAS‐mounted and handheld optical sensors; and (iii) evaluate thermal data's relationship to spectral reflectance from sUAS‐mounted and handheld sensors, soil volumetric water content (VWC), soil temperature, turfgrass visual quality (VQ), and percentage green cover (PGC). The study was conducted during summer 2017 on creeping bentgrass (Agrostis stolonifera L.) irrigated with 15 to 100% evapotranspiration (ET) replacements to impose a gradient of drought stress. Airborne spectral reflectance measurements included three bands (near infrared [NIR, 680–780 nm], green and blue bands [overlapped, 400–580 nm]) and eight derived vegetation indices. Results indicated Tc measurements via the sUAS detected rises of Tc in 15 and 30% compared with 100% ET plots, corresponding with declines in VWC, before drought stress became visible. This was comparable to the best spectral parameters on companion flights, and Tc was closely correlated with spectral data from sUAS‐mounted (|r| = 0.52–0.69) and handheld sensors (|r| = 0.75–0.82). Thermal data were more strongly correlated with turfgrass VQ (r = −0.60 to −0.77) and PGC (r = −0.58 to −0.78) than with VWC (r = −0.43 to −0.63) and soil temperature (|r| = 0.27–0.41).

Abstract Aberrant mitochondrial energy transfer underlies prevalent chronic health conditions, including cancer, cardiovascular, and neurodegenerative diseases. Mitochondrial transplantation represents an innovative strategy aimed at restoring favorable metabolic phenotypes in cells with dysfunctional energy metabolism. While promising, significant barriers to in vivo translation of this approach abound, including limited cellular uptake and recognition of mitochondria as foreign. The objective is to functionalize isolated mitochondria with a biocompatible polymer to enhance cellular transplantation and eventual in vivo applications. Herein, it is demonstrated that grafting of a polymer conjugate composed of dextran with triphenylphosphonium onto isolated mitochondria protects the organelles and facilitates cellular internalization compared with uncoated mitochondria. Importantly, mitochondrial transplantation into cancer and cardiovascular cells has profound effects on respiration, mediating a shift toward improved oxidative phosphorylation, and reduced glycolysis. These findings represent the first demonstration of polymer functionalization of isolated mitochondria, highlighting a viable strategy for enabling clinical applications of mitochondrial transplantation.

BackgroundLimited information exists on the role of B‐cell‐dependent mechanisms in the progression of heart failure (HF). However, in failing human myocardium, there is evidence of deposition of activated complement components as well as anticardiac antibodies. We aimed to determine the contribution of B‐cells in HF progression using a nonsurgical mouse model of nonischemic cardiomyopathy (CMP). Methods and ResultsCMP protocol involved the use of l‐NAME and NaCl in the drinking water and angiotensin‐II infusion for 35 days. At day 35, mice were analyzed by cardiac magnetic resonance imaging, gene expression, and histology. Mice (12 weeks old) were divided into 4 groups, all in C57BL/6 background: wild‐type (WT) CMP; severe combined immunodeficiency (SCID) CMP (T‐ and B‐cell deficient); CD22− CMP (B‐cell depleted); and Nude CMP (T‐cell deficient), with their respective controls. We performed B‐cell depletion and reconstitution protocols. The protective effect of B‐cell depletion was demonstrated by a significant reduction of cell hypertrophy and collagen deposition and a preserved ejection fraction in the CD22− CMP group compared to WT CMP. Once SCID mice underwent B‐cell reconstitution with isolated CMP B‐cells, the CMP phenotype was restored. Furthermore, deposition of IgG3 and apoptosis in the myocardium follows the development of CMP; in addition, in vitro studies demonstrated that activated B‐cells stimulate collagen production by cardiac fibroblasts. ConclusionsThe absence of B‐cells in this model of HF resulted in less hypertrophy and collagen deposition, preservation of left ventricular function, and, in association with these changes, a reduction in expression of proinflammatory cytokines, immunoglobulin G deposition, and apoptosis in the myocardium. Taken together, these data suggest that B‐cells play a contributory role in an angiotensin‐II‐induced HF model.

Abstract Introduction and Objectives Patient‐centred (PC) and holistic care improves patient satisfaction and health outcomes. We sought to investigate the benefit of utilising a PC pathology report in patients undergoing radical prostatectomy (RP) for prostate cancer (PCa). Our study aimed to evaluate and compare patient understanding of their PCa diagnosis after RP, upon receiving either a standard histopathology report or a personalised and PC report (PCR). Moreover, we evaluated knowledge retention at 4 weeks after the initial consultation. Methods We invited patients undergoing RP at three metropolitan Urology clinics to participate in our randomised controlled study. Patients were randomised to receive either a PCR or standard pathology report. Patient satisfaction questionnaires (Perceived Efficacy in Patient–Physician Interactions [PEPPI], Consultation and Relational Empathy [CARE] and Communication Assessment Tool [CAT]) and a knowledge test were conducted within 72 h of the initial appointment and again at 4 weeks. Accurate recollection of Gleason grade group (GGG) and extracapsular extension (ECE) were classified as ‘correct’. Baseline demographic data included age, education, marital and employment status, pre‐op prostate specific antigen (PSA) and clinical stage. Baseline data were tested for differences between groups using the Student's t test, chi‐squared test or Fisher's exact test depending on whether data were continuous, categorical or sparse. Comparison of correctly answered ‘knowledge’ questions was analysed using chi‐squared test. A significance level of p ≤ 0.05 was used. Results Data from 62 patients were analysed (30 standard vs. 32 PCR). No significant differences in baseline demographics were found between groups. Both groups reported high levels of satisfaction with their healthcare experiences in all domains of patient–physician rapport, empathy and communication. There were no significant differences between groups in PEPPI (p = 0.68), CAT (p = 0.39) and CARE (p = 0.66) scores, at baseline and 4 weeks. Ninety‐three per cent of patients who received the PCR understood the report while 90% felt the report added to their understanding of their PCa. Regarding patient knowledge, the PCR group had significantly more correct answers on GGG and ECE as compared with the standard report group at baseline and 4 weeks (p