1. Expression of Bcl-x, Bcl-2, Bax, and Bak in endarterectomy and atherectomy specimens
- Author
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Anurag Saxena, Dorothy Thomson, and J. D. McMeekin
- Subjects
Atherectomy, Coronary ,Pathology ,medicine.medical_specialty ,Arteriosclerosis ,bcl-X Protein ,Apoptosis ,Bcl-xL ,Pathology and Forensic Medicine ,Renal Artery ,Bcl-2-associated X protein ,Western blot ,Proto-Oncogene Proteins ,In Situ Nick-End Labeling ,medicine ,Humans ,bcl-2-Associated X Protein ,Endarterectomy, Carotid ,TUNEL assay ,biology ,medicine.diagnostic_test ,Karyorrhexis ,Membrane Proteins ,Muscle, Smooth ,Coronary Vessels ,Carotid Arteries ,bcl-2 Homologous Antagonist-Killer Protein ,Proto-Oncogene Proteins c-bcl-2 ,biology.protein ,Biomarkers ,Bcl-2 Homologous Antagonist-Killer Protein ,Pyknosis - Abstract
The regulation of apoptosis in atherosclerosis is not completely defined. The aim of this study was to determine the expression of Bcl-2, Bcl-x, Bax, and Bak in relation to apoptosis in advanced atherosclerotic lesions. In atherectomy (15), endarterectomy (10), and control non-atherosclerotic segments of renal (2) and of coronary and carotid (5) arteries, the extent of apoptosis was determined using TdT dUTP nick end labelling (TUNEL) and nuclear morphology (karyorrhexis/pyknosis) and expression of apoptosis regulators by immunohistochemistry and western blot analysis on paraffin-embedded material. In all specimens, the atherosclerotic involvement was advanced: grade V (n=18) and grade VI (n=7). The apoptotic index was high (mean 30%) in advanced lesions compared with controls (
- Published
- 2002
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