1. Nanoengineering hybrid supramolecular multilayered biomaterials using polysaccharides and self-assembling peptide amphiphiles
- Author
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Goksu Cinar, Maria P. Sousa, João Borges, João F. Mano, Mustafa O. Guler, Sofia G. Caridade, and Güler, Mustafa O.
- Subjects
Nanofibers ,02 engineering and technology ,Optical microscopy ,01 natural sciences ,Molecular weight ,Morphological properties ,Biopolymers ,Tissue engineering ,Biomimetics ,Electrochemistry ,Peptide amphiphiles ,Supramolecular nanostructured multilayered biomaterials ,Self-assembly ,Self assembly ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Layer-by-layer assemblies ,3. Good health ,Electronic, Optical and Magnetic Materials ,Electrostatically driven ,Drug delivery ,0210 nano-technology ,Self-assembling peptide ,Materials science ,Supramolecular chemistry ,Nanotechnology ,Nanoengineering ,010402 general chemistry ,Biomaterials ,Low-molecular-weight peptides ,Multi-layered ,Polysaccharides ,Deposition ,Ions ,Biotechnological applications ,Layer-by-layer assembly ,Self-assembling peptides ,Alginate ,technology, industry, and agriculture ,Cell adhesion ,Quartz crystal microbalance ,Amphiphiles ,Molecules ,0104 chemical sciences ,Nanostructures ,Nanofiber ,Cell culture ,Peptides - Abstract
Developing complex supramolecular biomaterials through highly dynamic and reversible noncovalent interactions has attracted great attention from the scientific community aiming key biomedical and biotechnological applica-tions, including tissue engineering, regenerative medicine, or drug delivery. In this study, the authors report the fabrication of hybrid supramolecular multilayered biomaterials, comprising high-molecular-weight biopolymers and oppositely charged low-molecular-weight peptide amphiphiles (PAs), through combination of self-assembly and electrostatically driven layer-by-layer (LbL) assembly approach. Alginate, an anionic polysaccharide, is used to trigger the self-assembling capability of positively charged PA and formation of 1D nanofiber networks. The LbL technology is further used to fabricate supramolecular multilayered biomaterials by repeating the alternate deposi-tion of both molecules. The fabrication process is monitored by quartz crystal microbalance, revealing that both materials can be successfully combined to conceive stable supramolecular systems. The morphological properties of the systems are studied by advanced microscopy techniques, revealing the nano-structured dimensions and 1D nanofibrous network of the assembly formed by the two molecules. Enhanced C2C12 cell adhesion, proliferation, and differentiation are observed on nanostructures having PA as outermost layer. Such supramolecular biomaterials demonstrate to be innovative matrices for cell culture and hold great potential to be used in the near future as prom-ising biomimetic supramolecular nanoplatforms for practical applications.
- Published
- 2017