10 results on '"G, Ito"'
Search Results
2. Electromyographic examination of jaw muscles in relation to symptoms and occlusion of patients with temporomandibular joint disorders
- Author
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G. Ito, Zi Jun Liu, K. Yamagata, and Yasuo Kasahara
- Subjects
Adult ,Male ,medicine.medical_specialty ,Refractory Period, Electrophysiological ,Movement ,Muscle Relaxation ,Pain ,Temporal Muscle ,Electromyography ,Temporal muscle ,Bite Force ,Dental Occlusion ,Masseter muscle ,Physical medicine and rehabilitation ,Reaction Time ,medicine ,Humans ,General Dentistry ,medicine.diagnostic_test ,Muscle fatigue ,Masseter Muscle ,Dental occlusion ,Vertical Dimension ,Temporomandibular Joint Disorders ,Adaptation, Physiological ,Temporomandibular joint ,stomatognathic diseases ,medicine.anatomical_structure ,Muscle relaxation ,Muscle Tonus ,Masticatory Muscles ,Muscle Fatigue ,Physical therapy ,Female ,Silent period ,Psychology ,Muscle Contraction - Abstract
Clinical/occlusal scores and jaw-muscle EMGs were recorded in 24 TMD symptomatic (group S) and 20 normal (group N) subjects to evaluate the significance of EMG parameters and their clinical associations. Results indicated: (1) integrated EMG activity (IEMG) was larger at the rest position (RP) in anterior temporalis (Ta) but smaller at maximal voluntary clenching (MVC) in masseter (Ma) and Ta, and the ratios of IEMG at 70%MVC to the corresponding bite force (70%BF) were greater in group S; (2) mean power frequency (MPF) were almost the same in both groups but its shift was more rapid in group S; (3) silent period duration (SPD) was longer in group S; (4) asymmetry indices for SPD and silent period latency (SPL) were larger in group S; (5) muscle pain was associated negatively with IEMG at MVC and 70%BF but positively with IEMGs at RP and 70%MVC, and impaired jaw movements were associated negatively with the above EMG values; (6) muscle pain was positively associated with SPD in Ma, while joint pain and sound showed positive and negative associations with SPD, respectively; (7) associations between occlusion and EMG parameters were found more in group N. These findings verify: (1) jaw elevators in TMD may have hyper-tonic activities and a weak functional efficiency; (2) jaw muscles in TMD may become easily fatigued following a functional effort, and less relaxed following a muscle twitch; (3) the severity of pain could not be reflected in EMG activities, but impaired jaw movement may increase tonic activity and decrease functional effort; (4) TMD symptoms may alter the functional adaptation of jaw-muscle activities and occlusion.
- Published
- 1999
3. Trypsin-like protease of mites: purification and characterization of trypsin-like protease from mite faecal extract Dermatophagoides farinae. Relationship between trypsin-like protease and Der f III
- Author
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M. Iwaki, S. Ikeda, A Miyahara, R. Homma, Toru Ando, Michiko Haida, H Kimura, G Ito, H. Yamakawa, Matsunobu Suko, T. Yanagihara, Yasushi Okumura, Y. Ino, and Hirokazu Okudaira
- Subjects
Male ,medicine.medical_treatment ,Guanidines ,Substrate Specificity ,Feces ,chemistry.chemical_compound ,Immunology and Allergy ,Aprotinin ,Amino Acids ,Peptide sequence ,chemistry.chemical_classification ,Mites ,biology ,Serine Endopeptidases ,Temperature ,Hydrogen-Ion Concentration ,Middle Aged ,Chromatography, Ion Exchange ,Amino acid ,Biochemistry ,Chromatography, Gel ,Female ,Trypsin Inhibitors ,medicine.drug ,Adult ,Serine Proteinase Inhibitors ,Adolescent ,Iodoacetic acid ,Trypsin inhibitor ,Molecular Sequence Data ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Arthropod Proteins ,medicine ,Animals ,Humans ,Amino Acid Sequence ,Aged ,Serine protease ,Protease ,Sequence Homology, Amino Acid ,Allergens ,Antigens, Plant ,Benzamidines ,Molecular Weight ,Enzyme ,chemistry ,biology.protein ,Digestive System ,Sequence Alignment - Abstract
Summary A serine protease from mite faecal extract, Dermatophagoides farinae, was purified using DEAE-Sephacel anion exchange chromatography and Supcrdex 75 pg gel chromato-graphy. The molecular weight of this protease was 34 kD on SDS-PAGE under reducing conditions. The optimal pH and temperature of the protease were 8-0 and 47 C, respectively. In addition, this protease cleaved arginyl or lysyl residue containing substrates selectively and was only inhibited by aprotinin, PUT-175, tind soy bean trypsin inhibitor and not by chymostatin, E-64 and iodoacetic acid. These results show that our purified serine protease belongs to the trypsin-type. Purified trypsin-like protease was shown to be allergenic by enzyme-linked immunosorbent assay. Antigeni-city of trypsin-like protease was completely different from those of Der f I and Der f II. Both, 20 N-terminal amino acid sequence and amino acid compositions of the purified protease were very similar to those of Der f III. Good similarities were found between trypsin-like protease and Der f III concerning physicochemical properties such as molecular weight on SDS-PAGE and ammonium sulphate solubility. Summarizing the above data, it can be concluded that a trypsin-like protease from mite faecal extract is actually the Der f III allergen and that it may be involved in the digestive process of the mite as it was found not in mite body but in mite faeces.
- Published
- 1993
4. Comparison of the Effectiveness of Baloxavir and Oseltamivir in Outpatients With Influenza B.
- Author
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Takazono T, Ito G, Hosogaya N, Iwanaga N, Komeda T, Kobayashi M, Kitanishi Y, Ogura E, and Mukae H
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- Humans, Male, Retrospective Studies, Female, Middle Aged, Adult, Aged, Young Adult, Adolescent, Hospitalization statistics & numerical data, Child, Pyridines therapeutic use, Japan epidemiology, Child, Preschool, Treatment Outcome, Infant, Aged, 80 and over, Influenza, Human drug therapy, Dibenzothiepins therapeutic use, Oseltamivir therapeutic use, Antiviral Agents therapeutic use, Pyridones therapeutic use, Morpholines therapeutic use, Triazines therapeutic use, Outpatients, Influenza B virus drug effects
- Abstract
This retrospective cohort study analyzed data from a Japanese health insurance database to assess the effectiveness of baloxavir (n = 4822) for preventing severe events compared with oseltamivir (n = 10,523) in patients with influenza B. The primary endpoint was hospitalization incidence (Days 2-14). The secondary endpoints included intravenous antibacterial drug use, pneumonia hospitalization, heart failure hospitalization, inhalational oxygen requirement, and use of other anti-influenza drugs. The hospitalization incidence was significantly lower with baloxavir (0.15% vs. 0.37%; risk ratio: 2.48, 95% confidence interval: 1.13-5.43). Pneumonia and additional anti-influenza therapy were also less frequent with baloxavir, thus supporting its use. Trial Registration: UMIN Clinical Trials Registry Study ID: UMIN000051382., (© 2024 The Author(s). Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.)
- Published
- 2024
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5. Participatory approaches for developing a practical handbook integrating health information for supporting individuals with mild cognitive impairment and their families.
- Author
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Kuroda Y, Goto A, Sugimoto T, Fujita K, Uchida K, Matsumoto N, Shimada H, Ohtsuka R, Yamada M, Fujiwara Y, Seike A, Hattori M, Ito G, Arai H, and Sakurai T
- Subjects
- Humans, Female, Male, Aged, Life Style, Family psychology, Interviews as Topic, Qualitative Research, Middle Aged, Aged, 80 and over, Cognitive Dysfunction therapy, Health Literacy
- Abstract
Aim: This study aimed to develop a patient-centred handbook that integrates information on lifestyle modifications and psychological support strategies for individuals with mild cognitive impairment (MCI). This article provides a comprehensive record of the development process., Methods: We adopted a participatory research model for the methodology, which comprised five phases and involved an interdisciplinary team specializing in dementia and health literacy. Data were initially collected via interviews conducted among patients with MCI (n = 5) and their families (n = 5). Given the study's preliminary nature, depth and richness of the qualitative data were the key concerns for determining the sample size, rather than broad generalizability. We ensured the inclusion of diverse experiences and perspectives by facilitating the creation of patient questions (PQs) that merged scientific evidence with patient perspectives. To enhance the handbook's accessibility and utility, we continuously evaluated the same using patient interviews, health literacy tool assessments and team discussions. This comprehensive approach harmonized scientific knowledge and patient experience, leading to the development of a personalized MCI management guide., Results: The handbook comprises nine domains, encompassing 38 selected PQs: MCI, lifestyle, lifestyle-related diseases, exercise, nutrition, social participation, cognitive training, psychological care and family support. The health literacy handbook was evaluated based on Clear Communication Index scores. The results revealed that 73.7% of the PQs were deemed difficult prerevision, whereas only 5.3% remained challenging postrevision. The formative evaluation underscored the need for more detailed explanations prerevision, whereas postrevision comments focused primarily on editorial suggestions., Conclusion: The inclusion of patients' perspectives right from the outset ensured that the handbook met their specific needs. The final version, which reflects all stakeholders' inputs, is now slated for imminent publication., Patient or Public Contribution: Patients and the public participated extensively throughout the project, from initial interviews to material evaluation and refinement., (© 2023 The Authors. Health Expectations published by John Wiley & Sons Ltd.)
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- 2024
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6. P2X7 receptor in the trigeminal sensory nuclear complex contributes to tactile allodynia/hyperalgesia following trigeminal nerve injury.
- Author
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Ito G, Suekawa Y, Watanabe M, Takahashi K, Inubushi T, Murasaki K, Hirose N, Hiyama S, Uchida T, and Tanne K
- Subjects
- Animals, Behavior, Animal physiology, Blotting, Western, Brain Stem chemistry, Brain Stem metabolism, Constriction, Pathologic, Facial Pain etiology, Facial Pain physiopathology, Immunohistochemistry, Injections, Spinal, Male, Microglia metabolism, Peripheral Nerves drug effects, Purinergic P2X Receptor Agonists pharmacology, Purinergic P2X Receptor Antagonists pharmacology, Rats, Rats, Sprague-Dawley, Real-Time Polymerase Chain Reaction, Receptors, Purinergic P2X7 drug effects, Receptors, Purinergic P2X7 genetics, Tumor Necrosis Factor-alpha biosynthesis, Up-Regulation drug effects, p38 Mitogen-Activated Protein Kinases metabolism, Hyperalgesia physiopathology, Receptors, Purinergic P2X7 physiology, Trigeminal Nerve Injuries physiopathology, Trigeminal Nuclei physiopathology
- Abstract
Background: The present study directly addresses the roles of the P2X(7) receptor (P2X(7)R), an ionotropic adenosine triphosphate (ATP) receptor, and cytokines in the induction of orofacial pain following chronic constriction injury (CCI) of the infraorbital nerve (IoN)., Methods: Rats were anesthetized, and ligatures of 4-0 chromic gut were tied around the IoN. A438079, a P2X(7)R antagonist or SB203580, a phosphorylated (p)-p38 mitogen-activated protein kinase (MAPK) inhibitor, was infused intrathecally into CCI-treated rats. In another group of rats, 3'-O-(4-benzoylbenzoyl) adenosine 5'-triphosphate (BzATP), a P2X(7) R agonist, was infused intrathecally with A438079, SB203580 or etanercept, a tumor necrosis factor (TNF)-α receptor-binding recombinant drug., Results: CCI of the IoN induced tactile allodynia/hyperalgesia and up-regulation of P2X(7)R, membrane-bound TNF-α (mTNF-α) and soluble TNF-α (sTNF-α) in the trigeminal sensory nuclear complex (TNC). Tactile allodynia/hyperalgesia or up-regulation of mTNF-α and sTNF-α in the TNC following CCI of the IoN was inhibited by A438079. SB203580 also attenuated tactile allodynia/hyperalgesia or up-regulation of mTNF-α, but not the up-regulation of sTNF-α in the TNC. Treatment of rats with BzATP induced tactile allodynia/hyperalgesia and up-regulation of sTNF-α and p-p38 in the TNC. Tactile allodynia/hyperalgesia or up-regulation of sTNF-α following BzATP treatment was inhibited by SB203580 and etanercept., Conclusions: Based on these findings, phosphorylation of p38 MAPK via P2X(7)R may induce tactile allodynia/hyperalgesia, which is most likely mediated by sTNF-α released by microglia., (© 2012 European Federation of International Association for the Study of Pain Chapters.)
- Published
- 2013
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7. IL-1beta in the trigeminal subnucleus caudalis contributes to extra-territorial allodynia/hyperalgesia following a trigeminal nerve injury.
- Author
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Takahashi K, Watanabe M, Suekawa Y, Ito G, Inubushi T, Hirose N, Murasaki K, Hiyama S, Uchida T, and Tanne K
- Subjects
- Animals, Antirheumatic Agents pharmacology, Astrocytes pathology, Disease Models, Animal, Dizocilpine Maleate pharmacology, Excitatory Amino Acid Antagonists pharmacology, Hyperalgesia drug therapy, Hyperalgesia pathology, Injections, Spinal, Interleukin 1 Receptor Antagonist Protein pharmacology, Mandible innervation, Microglia pathology, Rats, Trigeminal Caudal Nucleus pathology, Up-Regulation physiology, Vibrissae innervation, Astrocytes metabolism, Hyperalgesia metabolism, Interleukin-1beta metabolism, Microglia metabolism, Trigeminal Caudal Nucleus metabolism, Trigeminal Nerve Injuries
- Abstract
It has been reported that the whisker pad (WP) area, which is innervated by the second branch of the trigeminal nerve, shows allodynia/hyperalgesia following transection of the mental nerve (MN: the third branch of the trigeminal nerve). However, the mechanisms of this extra-territorial pain induction still remain unclear. Glia and cytokines are known to facilitate perception of noxious input, raising a possibility that these non-neuronal elements are involved in the induction and spread of allodynia/hyperalgesia at non-injured skin territory. One day after MN transection, tactile allodynia/hyperalgesia developed on the ipsilateral WP area, which is in the non-injured skin territory. The tactile allodynia/hyperalgesia lasted for more than 56 days. In response to MN transection, astrocytes and microglia appeared to be in an activated state, and interleukin (IL)-1beta was up-regulated in astrocytes in the trigeminal subnucleus caudalis (Vc). Allodynia/hyperalgesia at WP area induced by MN transection was attenuated dose-dependently by IL-1 receptor antagonist IL-1ra (i.t., 0.05, 0.5, and 5 pg/rat). Fos-like immunoreactive (Fos-Li) neurons were observed in the Vc after non-noxious mechanical stimulation of the WP area in the rats with MN transection. Administration of IL-1ra also attenuated the number of Fos-Li neurons dose-dependently. Administration of a noncompetitive antagonist of NMDA receptors MK-801 (i.t., 5 μg/rat) reversed allodynia/hyperalgesia. IL-1 receptor type I (IL-1RI) was localized in Fos- and phospho NR1-immunoreactive neurons. These results suggest that IL-1beta in the Vc plays an important role in the development of extra-territorial tactile allodynia/hyperalgesia after MN transection., (Copyright © 2010 European Federation of International Association for the Study of Pain Chapters. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2011
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8. p53 apoptotic pathway molecules are frequently and simultaneously altered in nonsmall cell lung carcinoma.
- Author
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Mori S, Ito G, Usami N, Yoshioka H, Ueda Y, Kodama Y, Takahashi M, Fong KM, Shimokata K, and Sekido Y
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- Adult, Aged, Aged, 80 and over, Female, Humans, Immunohistochemistry, Male, Middle Aged, Neoplasm Proteins, Nuclear Proteins, Polymerase Chain Reaction, Polymorphism, Single-Stranded Conformational, Proto-Oncogene Proteins metabolism, Proto-Oncogene Proteins pharmacology, Proto-Oncogene Proteins c-mdm2, Tumor Suppressor Protein p14ARF metabolism, Tumor Suppressor Protein p14ARF pharmacology, Tumor Suppressor Protein p53 metabolism, Tumor Suppressor Protein p53 pharmacology, Zinc Fingers, Apoptosis, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung physiopathology, Gene Expression Regulation, Neoplastic, Lung Neoplasms genetics, Lung Neoplasms physiopathology, Proto-Oncogene Proteins genetics, Tumor Suppressor Protein p14ARF genetics, Tumor Suppressor Protein p53 genetics
- Abstract
Background: Lung carcinomas show frequent inactivation of the p53 tumor suppressor, which regulates an apoptotic pathway. The objective of the current study was to assess how the p53 apoptotic pathway is altered in nonsmall cell lung carcinoma (NSCLC), especially in tumors without p53 alterations., Methods: p53, its upstream regulators (p14(ARF) and HDM2), and downstream effectors of the apoptotic pathway (BAX and BCL2) were studied in 118 NSCLC specimens. p53 was analyzed by single-stranded conformation polymorphism analysis covering exons 2-11 and by immunohistochemistry (IHC). p14(ARF) was analyzed by methylation-specific polymerase chain reaction and IHC. HDM2 was analyzed using Southern blot analysis and IHC. BAX and BCL2 were analyzed by IHC. Two other upstream regulators that regulate the stability of HDM2, PTEN and HAUSP, also were studied., Results: Of 118 NSCLC specimens that were analyzed, p53 alterations were detected in 74 tumors (63%), p14(ARF) inactivation was detected in 53 tumors (45%), and overexpression of HDM2 was found in 31 tumors (26%), including 6 tumors with gene amplification. Although p53 inactivation and HDM2 overexpression were detected simultaneously, HDM2 gene amplification was observed only in tumor specimens without p53 mutations. IHC revealed PTEN down-regulation in 22 of 88 tumors (25%). HAUSP Northern blot analysis demonstrated several-fold differences in gene expression that did not correlate with p53 alterations. Of 118 NSCLC specimens, expression of BAX and BCL2 expression were detected in 46 tumors (39%) and 17 tumors (14%), respectively. Finally, ASPP1 and ASPP2, molecules involved in mediating the transcription function of p53, were not found to be aberrantly expressed when tested by Northern blot analysis., Conclusions: Overall, two or more p53 pathway components were found to be frequently altered in patients with NSCLC. Greater than 90% of the alterations were due to abnormalities of p53, p14(ARF), or HDM2. Therefore, the inactivation of one or more components of the p53 pathway appears to be a prerequisite for the development of most NSCLCs., (Copyright 2004 American Cancer Society.)
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- 2004
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9. The influence of bioglass on the growth of fibroblasts.
- Author
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Matsuda T, Yamauchi K, and Ito G
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- Animals, Biocompatible Materials, Cell Division, Mice, Microscopy, Electron, Scanning, Ceramics, L Cells cytology
- Abstract
The influence of Bioglass (BG) on the growth of L cells was studied by means of tissue culture, to get an idea on its bone-bonding mechanism. Growth rate of L cells on BG was lower than that on the control silica glass (SG). L cells on BG diminished in size and took up long and slender polygonal shapes with radially spread long pseudopods, which probably meant that the cell cycle was detained at the synthetic period. From these findings, BG is thought to have an inhibitory effect on the growth of L cells, which might arrest the fibrous tissue encapsulation and allow bonding with bone.
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- 1987
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10. A histological comparison of the tissue interface of bioglass and silica glass.
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Ito G, Matsuda T, Inoue N, and Kamegai T
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- Animals, Biocompatible Materials, Bone Cements, Dental Implantation, Foreign-Body Reaction etiology, Glass, Male, Microscopy, Electron, Scanning, Rabbits, Silicon Dioxide, Bone and Bones pathology, Ceramics, Prostheses and Implants adverse effects
- Abstract
The objectives of the present article are to confirm the bone bonding phenomenon of Bioglass (BG) developed by Hench et al., and to observe the singularity of tissue reaction to it. BG and nonreactive silica glass (SG) were implanted in the femurs of rabbits and rats. Histological examination revealed that a relatively acellular zone with little inflammation was formed on BG surface at 1 day after implantation. Neither fibrous tissue nor a distinct boundary was observed between BG and bone after 7 days. On the contrary, a moderate postoperative inflammatory reaction was observed on SG at 1 day, and fibrous tissue was observed between SG and bone after 7 days. From these findings, it was confirmed that BG bonded directly with bone. As the relatively acellular zone observed on BG surface at 1 day was replaced by bone after 7 days, the formation of this zone might play an important role in bone-bonding process. Further research should be focused on the mechanism and biological meaning of bone bonding, for this phenomenon can not be explained by the conventional pathological theory of foreign body encapsulation.
- Published
- 1987
- Full Text
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